Restriction map for
pF1KA1204
(Linear) (Six-base) MAPSORT of: KIAA1204.seq Check: 9383 from: 1 to: 4332
KIAA1204.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 594 2355 4085 4332
Size: 594 1761 1730 247
Fragments arranged by size:
1761 1730 594 247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 459 1315 1712 2050 2485 2639 2785
Size: 389 70 856 397 338 435 154 146
Cuts at: 2785 2944 3003 4332
Size: 159 59 1329
Fragments arranged by size:
1329 856 435 397 389 338 159 154
146 70 59
AgeI A'CCGG_T
Cuts at: 0 1108 4332
Size: 1108 3224
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 83 115 4332
Size: 83 32 4217
Fragments arranged by size:
4217 83 32
AlwNI CAG_nnn'CTG
Cuts at: 0 27 1060 1648 1690 1977 2232 2921 3492
Size: 27 1033 588 42 287 255 689 571
Cuts at: 3492 3538 3645 3684 4332
Size: 46 107 39 648
Fragments arranged by size:
1033 689 648 588 571 287 255 107
46 42 39 27
ApaI G_GGCC'C
Cuts at: 0 2075 3290 4332
Size: 2075 1215 1042
Fragments arranged by size:
2075 1215 1042
ApaLI G'TGCA_C
Cuts at: 0 656 4332
Size: 656 3676
ApoI r'AATT_y
Cuts at: 0 127 340 1086 2541 2946 4332
Size: 127 213 746 1455 405 1386
Fragments arranged by size:
1455 1386 746 405 213 127
AvaI C'yCGr_G
Cuts at: 0 87 1213 2056 2899 4332
Size: 87 1126 843 843 1433
Fragments arranged by size:
1433 1126 843 843 87
BamHI G'GATC_C
Cuts at: 0 1317 2160 4332
Size: 1317 843 2172
Fragments arranged by size:
2172 1317 843
BanI G'GyrC_C
Cuts at: 0 522 841 1150 1741 2970 4332
Size: 522 319 309 591 1229 1362
Fragments arranged by size:
1362 1229 591 522 319 309
BanII G_rGCy'C
Cuts at: 0 277 332 1424 1466 1786 1888 2006 2075
Size: 277 55 1092 42 320 102 118 69
Cuts at: 2075 3290 3661 4332
Size: 1215 371 671
Fragments arranged by size:
1215 1092 671 371 320 277 118 102
69 55 42
BbeI G_GCGC'C
Cuts at: 0 526 1745 4332
Size: 526 1219 2587
Fragments arranged by size:
2587 1219 526
BbsI GAAGACnn'nnnn_
Cuts at: 0 1386 2038 2426 4032 4332
Size: 1386 652 388 1606 300
Fragments arranged by size:
1606 1386 652 388 300
BbvCI CC'TCA_GC
Cuts at: 0 1220 4283 4332
Size: 1220 3063 49
Fragments arranged by size:
3063 1220 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1450 1484 1527 1561 4332
Size: 1450 34 43 34 2771
Fragments arranged by size:
2771 1450 43 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2254 4332
Size: 2254 2078
BglII A'GATC_T
Cuts at: 0 231 2445 2486 4332
Size: 231 2214 41 1846
Fragments arranged by size:
2214 1846 231 41
BlpI GC'TnA_GC
Cuts at: 0 17 4332
Size: 17 4315
Bme1580I G_kGCm'C
Cuts at: 0 660 844 2075 3155 3290 4332
Size: 660 184 1231 1080 135 1042
Fragments arranged by size:
1231 1080 1042 660 184 135
BmrI ACTGGGnnnn_n'
Cuts at: 0 2727 3595 4332
Size: 2727 868 737
Fragments arranged by size:
2727 868 737
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 231 2022 2628 2876 2925 4332
Size: 231 1791 606 248 49 1407
Fragments arranged by size:
1791 1407 606 248 231 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 691 876 1243 1771 2111 2164 2369
Size: 238 453 185 367 528 340 53 205
Cuts at: 2369 2392 2725 2898 3263 3483 3706 3851 4332
Size: 23 333 173 365 220 223 145 481
Fragments arranged by size:
528 481 453 367 365 340 333 238
223 220 205 185 173 145 53 23
Bpu10I CC'TnA_GC
Cuts at: 0 199 1220 1268 2222 3660 4283 4332
Size: 199 1021 48 954 1438 623 49
Fragments arranged by size:
1438 1021 954 623 199 49 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 769 883 1690 1800 4332
Size: 743 26 114 807 110 2532
Fragments arranged by size:
2532 807 743 114 110 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 671 3405 3407 4249 4332
Size: 671 2734 2 842 83
Fragments arranged by size:
2734 842 671 83 2
BsaHI Gr'CG_yC
Cuts at: 0 523 1742 4332
Size: 523 1219 2590
Fragments arranged by size:
2590 1219 523
BsaWI w'CCGG_w
Cuts at: 0 1108 1284 1320 1821 4332
Size: 1108 176 36 501 2511
Fragments arranged by size:
2511 1108 501 176 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 340 370 1534 1564 3849 3877 3879 3907
Size: 340 30 1164 30 2285 28 2 28
Cuts at: 3907 4332
Size: 425
Fragments arranged by size:
2285 1164 425 340 30 30 28 28
2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 523 1768 1852 2011 2014 2249 2324 2342
Size: 523 1245 84 159 3 235 75 18
Cuts at: 2342 2357 2389 2524 2638 2901 3044 3705 3747
Size: 15 32 135 114 263 143 661 42
Cuts at: 3747 4332
Size: 585
Fragments arranged by size:
1245 661 585 523 263 235 159 143
135 114 84 75 42 32 18 15
3
BseYI C'CCAG_C
Cuts at: 0 1623 2693 2839 3093 3217 3706 4332
Size: 1623 1070 146 254 124 489 626
Fragments arranged by size:
1623 1070 626 489 254 146 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 1545 2218 3244 3818 4332
Size: 143 1402 673 1026 574 514
Fragments arranged by size:
1402 1026 673 574 514 143
BsiHKAI G_wGCw'C
Cuts at: 0 332 660 1786 2006 2179 3251 3277 3661
Size: 332 328 1126 220 173 1072 26 384
Cuts at: 3661 4332
Size: 671
Fragments arranged by size:
1126 1072 671 384 332 328 220 173
26
BsmI GAATG_Cn'
Cuts at: 0 1717 2556 3398 4332
Size: 1717 839 842 934
Fragments arranged by size:
1717 934 842 839
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2616 4332
Size: 2616 1716
Bsp1286I G_dGCh'C
Cuts at: 0 277 332 660 844 1424 1466 1786 1888
Size: 277 55 328 184 580 42 320 102
Cuts at: 1888 2006 2075 2179 3155 3251 3277 3290 3661
Size: 118 69 104 976 96 26 13 371
Cuts at: 3661 4332
Size: 671
Fragments arranged by size:
976 671 580 371 328 320 277 184
118 104 102 96 69 55 42 26
13
BspEI T'CCGG_A
Cuts at: 0 1320 1821 4332
Size: 1320 501 2511
Fragments arranged by size:
2511 1320 501
BspHI T'CATG_A
Cuts at: 0 695 4332
Size: 695 3637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 594 1654 2355 3387 3744 3896 4085 4332
Size: 594 1060 701 1032 357 152 189 247
Fragments arranged by size:
1060 1032 701 594 357 247 189 152
BsrBI CCG'CTC
Cuts at: 0 1485 1595 4332
Size: 1485 110 2737
Fragments arranged by size:
2737 1485 110
BsrDI GCAATG_nn'
Cuts at: 0 360 573 2687 3341 3922 4332
Size: 360 213 2114 654 581 410
Fragments arranged by size:
2114 654 581 410 360 213
BsrFI r'CCGG_y
Cuts at: 0 686 1108 1147 1153 4332
Size: 686 422 39 6 3179
Fragments arranged by size:
3179 686 422 39 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1523 2232 4332
Size: 1523 709 2100
Fragments arranged by size:
2100 1523 709
BstBI TT'CG_AA
Cuts at: 0 1369 4332
Size: 1369 2963
BstEII G'GTnAC_C
Cuts at: 0 2806 4332
Size: 2806 1526
BstXI CCAn_nnnn'nTGG
Cuts at: 0 738 2142 4332
Size: 738 1404 2190
Fragments arranged by size:
2190 1404 738
BstYI r'GATC_y
Cuts at: 0 231 1317 2160 2445 2486 3632 3795 4332
Size: 231 1086 843 285 41 1146 163 537
Fragments arranged by size:
1146 1086 843 537 285 231 163 41
Bsu36I CC'TnA_GG
Cuts at: 0 535 3010 4332
Size: 535 2475 1322
Fragments arranged by size:
2475 1322 535
BtgI C'CryG_G
Cuts at: 0 836 2876 4332
Size: 836 2040 1456
Fragments arranged by size:
2040 1456 836
BtsI GCAGTG_nn'
Cuts at: 0 359 1248 3830 4332
Size: 359 889 2582 502
Fragments arranged by size:
2582 889 502 359
DraI TTT'AAA
Cuts at: 0 1796 4332
Size: 1796 2536
DraIII CAC_nnn'GTG
Cuts at: 0 270 1523 3281 4332
Size: 270 1253 1758 1051
Fragments arranged by size:
1758 1253 1051 270
DrdI GACnn_nn'nnGTC
Cuts at: 0 3421 4332
Size: 3421 911
EaeI y'GGCC_r
Cuts at: 0 773 1603 1940 4242 4332
Size: 773 830 337 2302 90
Fragments arranged by size:
2302 830 773 337 90
EarI CTCTTCn'nnn_
Cuts at: 0 109 694 853 1414 1562 1607 1804 2615
Size: 109 585 159 561 148 45 197 811
Cuts at: 2615 2815 2963 3358 4332
Size: 200 148 395 974
Fragments arranged by size:
974 811 585 561 395 200 197 159
148 148 109 45
EcoICRI GAG'CTC
Cuts at: 0 330 1784 2004 3659 4332
Size: 330 1454 220 1655 673
Fragments arranged by size:
1655 1454 673 330 220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 389 459 691 876 1243 1315 1712
Size: 238 151 70 232 185 367 72 397
Cuts at: 1712 1771 2050 2111 2164 2369 2392 2485 2639
Size: 59 279 61 53 205 23 93 154
Cuts at: 2639 2725 2785 2898 2944 3003 3263 3483 3706
Size: 86 60 113 46 59 260 220 223
Cuts at: 3706 3851 4332
Size: 145 481
Fragments arranged by size:
481 397 367 279 260 238 232 223
220 205 185 154 151 145 113 93
86 72 70 61 60 59 59 53
46 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 371 1064 1729 2500 3121 4332
Size: 371 693 665 771 621 1211
Fragments arranged by size:
1211 771 693 665 621 371
EcoO109I rG'GnC_Cy
Cuts at: 0 431 2071 2072 2229 2796 3014 3287 3480
Size: 431 1640 1 157 567 218 273 193
Cuts at: 3480 3489 3703 3853 4110 4332
Size: 9 214 150 257 222
Fragments arranged by size:
1640 567 431 273 257 222 218 214
193 157 150 9 1
EcoRI G'AATT_C
Cuts at: 0 2946 4332
Size: 2946 1386
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 309 1927 4332
Size: 309 1618 2405
Fragments arranged by size:
2405 1618 309
HaeII r_GCGC'y
Cuts at: 0 526 1268 1450 1745 4332
Size: 526 742 182 295 2587
Fragments arranged by size:
2587 742 526 295 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 60 92 340 372 816 848 937 969
Size: 60 32 248 32 444 32 89 32
Cuts at: 969 983 1015 1230 1262 1534 1566 1803 1835
Size: 14 32 215 32 272 32 237 32
Cuts at: 1835 1849 1881 1993 2025 2145 2177 2272 2304
Size: 14 32 112 32 120 32 95 32
Cuts at: 2304 2430 2462 3739 3771 3970 4002 4332
Size: 126 32 1277 32 199 32 330
Fragments arranged by size:
1277 444 330 272 248 237 215 199
126 120 112 95 89 60 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 14 14
HincII GTy'rAC
Cuts at: 0 2381 4332
Size: 2381 1951
KasI G'GCGC_C
Cuts at: 0 522 1741 4332
Size: 522 1219 2591
Fragments arranged by size:
2591 1219 522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1047 2386 2795 4332
Size: 1047 1339 409 1537
Fragments arranged by size:
1537 1339 1047 409
MscI TGG'CCA
Cuts at: 0 775 1605 1942 4244 4332
Size: 775 830 337 2302 88
Fragments arranged by size:
2302 830 775 337 88
MslI CAynn'nnrTG
Cuts at: 0 1796 3430 4332
Size: 1796 1634 902
Fragments arranged by size:
1796 1634 902
MspA1I CmG'CkG
Cuts at: 0 1293 1623 2463 2921 3553 4268 4287 4332
Size: 1293 330 840 458 632 715 19 45
Fragments arranged by size:
1293 840 715 632 458 330 45 19
NarI GG'CG_CC
Cuts at: 0 523 1742 4332
Size: 523 1219 2590
Fragments arranged by size:
2590 1219 523
NcoI C'CATG_G
Cuts at: 0 836 4332
Size: 836 3496
NspI r_CATG'y
Cuts at: 0 495 3846 3933 4332
Size: 495 3351 87 399
Fragments arranged by size:
3351 495 399 87
PasI CC'CwG_GG
Cuts at: 0 1889 2720 4332
Size: 1889 831 1612
Fragments arranged by size:
1889 1612 831
PflMI CCAn_nnn'nTGG
Cuts at: 0 270 606 1923 3492 4332
Size: 270 336 1317 1569 840
Fragments arranged by size:
1569 1317 840 336 270
PfoI T'CCnGG_A
Cuts at: 0 254 404 2428 3798 4332
Size: 254 150 2024 1370 534
Fragments arranged by size:
2024 1370 534 254 150
PpuMI rG'GwC_Cy
Cuts at: 0 431 3014 3480 3489 3703 3853 4110 4332
Size: 431 2583 466 9 214 150 257 222
Fragments arranged by size:
2583 466 431 257 222 214 150 9
PspOMI G'GGCC_C
Cuts at: 0 2071 3286 4332
Size: 2071 1215 1046
Fragments arranged by size:
2071 1215 1046
PstI C_TGCA'G
Cuts at: 0 1550 1644 1880 4332
Size: 1550 94 236 2452
Fragments arranged by size:
2452 1550 236 94
PvuII CAG'CTG
Cuts at: 0 1293 2463 2921 3553 4268 4332
Size: 1293 1170 458 632 715 64
Fragments arranged by size:
1293 1170 715 632 458 64
SacI G_AGCT'C
Cuts at: 0 332 1786 2006 3661 4332
Size: 332 1454 220 1655 671
Fragments arranged by size:
1655 1454 671 332 220
SanDI GG'GwC_CC
Cuts at: 0 3703 4332
Size: 3703 629
SapI GCTCTTCn'nnn_
Cuts at: 0 109 694 4332
Size: 109 585 3638
Fragments arranged by size:
3638 585 109
SbfI CC_TGCA'GG
Cuts at: 0 1880 4332
Size: 1880 2452
SfcI C'TryA_G
Cuts at: 0 426 1546 1640 1876 4332
Size: 426 1120 94 236 2456
Fragments arranged by size:
2456 1120 426 236 94
SfoI GGC'GCC
Cuts at: 0 524 1743 4332
Size: 524 1219 2589
Fragments arranged by size:
2589 1219 524
SgrAI Cr'CCGG_yG
Cuts at: 0 1108 4332
Size: 1108 3224
SmaI CCC'GGG
Cuts at: 0 1215 4332
Size: 1215 3117
SmlI C'TyrA_G
Cuts at: 0 748 758 862 1669 1779 2056 2899 4332
Size: 748 10 104 807 110 277 843 1433
Fragments arranged by size:
1433 843 807 748 277 110 104 10
SspI AAT'ATT
Cuts at: 0 920 4332
Size: 920 3412
StuI AGG'CCT
Cuts at: 0 1724 3309 4332
Size: 1724 1585 1023
Fragments arranged by size:
1724 1585 1023
StyI C'CwwG_G
Cuts at: 0 434 836 1079 1679 4106 4332
Size: 434 402 243 600 2427 226
Fragments arranged by size:
2427 600 434 402 243 226
SwaI ATTT'AAAT
Cuts at: 0 1796 4332
Size: 1796 2536
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2252 2727 2988 3231 3643 3913 4153 4332
Size: 2252 475 261 243 412 270 240 179
Fragments arranged by size:
2252 475 412 270 261 243 240 179
TatI w'GTAC_w
Cuts at: 0 2643 3623 4332
Size: 2643 980 709
Fragments arranged by size:
2643 980 709
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 768 800 2559 2591 2719 2751 4332
Size: 768 32 1759 32 128 32 1581
Fragments arranged by size:
1759 1581 768 128 32 32 32
XbaI T'CTAG_A
Cuts at: 0 2862 4332
Size: 2862 1470
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3425 4332
Size: 3425 907
XhoI C'TCGA_G
Cuts at: 0 2056 2899 4332
Size: 2056 843 1433
Fragments arranged by size:
2056 1433 843
XmaI C'CCGG_G
Cuts at: 0 1213 4332
Size: 1213 3119
XmnI GAAnn'nnTTC
Cuts at: 0 1300 2270 4332
Size: 1300 970 2062
Fragments arranged by size:
2062 1300 970
Enzymes that do cut:
AarI AcuI AgeI AloI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NarI NcoI NspI
PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI
SanDI SapI SbfI SfcI SfoI SgrAI SmaI SmlI
SspI StuI StyI SwaI TaqII TatI TstI XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AleI AlfI AscI AseI AsiSI AvrII BaeI BciVI
BclI BfrBI BmgBI BmtI BsaAI BsaBI BsiEI BsiWI
BsrGI BssHII BssSI BstZ17I BtgZI ClaI CspCI EagI
EciI EcoRV FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI ScaI SexAI
SfiI SgfI SnaBI SpeI SphI SrfI Tth111I ZraI