Restriction map for
pF1KSDA1206
(Linear) (Six-base) MAPSORT of: KIAA1206.seq Check: 4738 from: 1 to: 5727
KIAA1206.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 390 1484 5727
Size: 390 1094 4243
Fragments arranged by size:
4243 1094 390
AccI GT'mk_AC
Cuts at: 0 4676 5727
Size: 4676 1051
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 5727
Size: 1192 4535
AfeI AGC'GCT
Cuts at: 0 4232 5727
Size: 4232 1495
AflIII A'CryG_T
Cuts at: 0 2109 5727
Size: 2109 3618
AgeI A'CCGG_T
Cuts at: 0 2530 5727
Size: 2530 3197
AhdI GACnn_n'nnGTC
Cuts at: 0 5258 5727
Size: 5258 469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4618 5044 5727
Size: 4618 426 683
Fragments arranged by size:
4618 683 426
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4519 4551 5727
Size: 4519 32 1176
Fragments arranged by size:
4519 1176 32
AlwNI CAG_nnn'CTG
Cuts at: 0 357 384 1152 1167 1545 1571 1590 1919
Size: 357 27 768 15 378 26 19 329
Cuts at: 1919 2322 2390 3849 4545 4899 5664 5727
Size: 403 68 1459 696 354 765 63
Fragments arranged by size:
1459 768 765 696 403 378 357 354
329 68 63 27 26 19 15
ApaI G_GGCC'C
Cuts at: 0 132 759 765 919 1021 1727 2462 2714
Size: 132 627 6 154 102 706 735 252
Cuts at: 2714 2812 3189 3712 4571 5727
Size: 98 377 523 859 1156
Fragments arranged by size:
1156 859 735 706 627 523 377 252
154 132 102 98 6
ApaLI G'TGCA_C
Cuts at: 0 3562 4303 5727
Size: 3562 741 1424
Fragments arranged by size:
3562 1424 741
AvaI C'yCGr_G
Cuts at: 0 235 247 504 758 885 1093 1177 4570
Size: 235 12 257 254 127 208 84 3393
Cuts at: 4570 4619 5438 5727
Size: 49 819 289
Fragments arranged by size:
3393 819 289 257 254 235 208 127
84 49 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 5364 5397 5727
Size: 5364 33 330
Fragments arranged by size:
5364 330 33
BamHI G'GATC_C
Cuts at: 0 3980 5727
Size: 3980 1747
BanI G'GyrC_C
Cuts at: 0 177 428 531 597 889 925 967 1761
Size: 177 251 103 66 292 36 42 794
Cuts at: 1761 1934 2076 2701 2824 3109 3450 4684 4910
Size: 173 142 625 123 285 341 1234 226
Cuts at: 4910 4973 5727
Size: 63 754
Fragments arranged by size:
1234 794 754 625 341 292 285 251
226 177 173 142 123 103 66 63
42 36
BanII G_rGCy'C
Cuts at: 0 132 759 765 919 1021 1727 2282 2462
Size: 132 627 6 154 102 706 555 180
Cuts at: 2462 2525 2572 2645 2714 2812 3065 3189 3228
Size: 63 47 73 69 98 253 124 39
Cuts at: 3228 3331 3351 3712 4571 4859 5500 5543 5727
Size: 103 20 361 859 288 641 43 184
Fragments arranged by size:
859 706 641 627 555 361 288 253
184 180 154 132 124 103 102 98
73 69 63 47 43 39 20 6
BbeI G_GCGC'C
Cuts at: 0 432 5727
Size: 432 5295
BbsI GAAGACnn'nnnn_
Cuts at: 0 3357 3598 4778 5257 5727
Size: 3357 241 1180 479 470
Fragments arranged by size:
3357 1180 479 470 241
BbvCI CC'TCA_GC
Cuts at: 0 464 3457 3955 4865 5727
Size: 464 2993 498 910 862
Fragments arranged by size:
2993 910 862 498 464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2994 3028 3454 3488 3610 3644 5727
Size: 2994 34 426 34 122 34 2083
Fragments arranged by size:
2994 2083 426 122 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1397 3262 5727
Size: 1397 1865 2465
Fragments arranged by size:
2465 1865 1397
BclI T'GATC_A
Cuts at: 0 5619 5727
Size: 5619 108
BglI GCCn_nnn'nGGC
Cuts at: 0 578 923 1025 1873 1882 1979 4065 5727
Size: 578 345 102 848 9 97 2086 1662
Fragments arranged by size:
2086 1662 848 578 345 102 97 9
BlpI GC'TnA_GC
Cuts at: 0 229 1326 2788 5537 5727
Size: 229 1097 1462 2749 190
Fragments arranged by size:
2749 1462 1097 229 190
Bme1580I G_kGCm'C
Cuts at: 0 132 299 322 536 759 765 892 919
Size: 132 167 23 214 223 6 127 27
Cuts at: 919 928 1021 1223 1727 1802 1939 2291 2462
Size: 9 93 202 504 75 137 352 171
Cuts at: 2462 2470 2714 2812 2827 3112 3189 3566 3712
Size: 8 244 98 15 285 77 377 146
Cuts at: 3712 4307 4571 4687 4915 5104 5637 5727
Size: 595 264 116 228 189 533 90
Fragments arranged by size:
595 533 504 377 352 285 264 244
228 223 214 202 189 171 167 146
137 132 127 116 98 93 90 77
75 27 23 15 9 8 6
BmgBI CAC'GTC
Cuts at: 0 1061 1791 1824 2660 3123 4530 5727
Size: 1061 730 33 836 463 1407 1197
Fragments arranged by size:
1407 1197 1061 836 730 463 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 1419 2278 2717 3059 5410 5727
Size: 1419 859 439 342 2351 317
Fragments arranged by size:
2351 1419 859 439 342 317
BmtI G_CTAG'C
Cuts at: 0 4231 5206 5727
Size: 4231 975 521
Fragments arranged by size:
4231 975 521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 211 795 4752 5541 5727
Size: 211 584 3957 789 186
Fragments arranged by size:
3957 789 584 211 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1174 1192 1304 1435 1471 1760 2185
Size: 627 547 18 112 131 36 289 425
Cuts at: 2185 2195 2224 2839 2929 3175 3901 3910 3988
Size: 10 29 615 90 246 726 9 78
Cuts at: 3988 4189 4267 4960 5596 5659 5727
Size: 201 78 693 636 63 68
Fragments arranged by size:
726 693 636 627 615 547 425 289
246 201 131 112 90 78 78 68
63 36 29 18 10 9
Bpu10I CC'TnA_GC
Cuts at: 0 464 2639 3457 3955 4737 4865 5727
Size: 464 2175 818 498 782 128 862
Fragments arranged by size:
2175 862 818 782 498 464 128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2736 3475 4747 5727
Size: 2736 739 1272 980
Fragments arranged by size:
2736 1272 980 739
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 635 3886 3899 4333 5727
Size: 13 622 3251 13 434 1394
Fragments arranged by size:
3251 1394 622 434 13 13
BsaAI yAC'GTr
Cuts at: 0 786 828 1731 4302 5727
Size: 786 42 903 2571 1425
Fragments arranged by size:
2571 1425 903 786 42
BsaHI Gr'CG_yC
Cuts at: 0 429 719 1055 4472 5339 5727
Size: 429 290 336 3417 867 388
Fragments arranged by size:
3417 867 429 388 336 290
BsaWI w'CCGG_w
Cuts at: 0 1400 2530 2922 5064 5727
Size: 1400 1130 392 2142 663
Fragments arranged by size:
2142 1400 1130 663 392
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 709 739 805 835 2476 2506 2902 2932
Size: 709 30 66 30 1641 30 396 30
Cuts at: 2932 3204 3234 4648 4678 4765 4795 4835 4865
Size: 272 30 1414 30 87 30 40 30
Cuts at: 4865 5547 5577 5727
Size: 682 30 150
Fragments arranged by size:
1641 1414 709 682 396 272 150 87
66 40 30 30 30 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 1573 2221 3228 3304 3565 3799 3802
Size: 76 1497 648 1007 76 261 234 3
Cuts at: 3802 4138 4186 4966 5008 5656 5727
Size: 336 48 780 42 648 71
Fragments arranged by size:
1497 1007 780 648 648 336 261 234
76 76 71 48 42 3
BseYI C'CCAG_C
Cuts at: 0 309 329 963 1021 1126 1304 2087 2270
Size: 309 20 634 58 105 178 783 183
Cuts at: 2270 2503 2804 2828 2999 3044 3188 4180 4278
Size: 233 301 24 171 45 144 992 98
Cuts at: 4278 5727
Size: 1449
Fragments arranged by size:
1449 992 783 634 309 301 233 183
178 171 144 105 98 58 45 24
20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 1561 2620 3148 3193 3700 3715 3736
Size: 16 1545 1059 528 45 507 15 21
Cuts at: 3736 3895 4855 5040 5224 5727
Size: 159 960 185 184 503
Fragments arranged by size:
1545 1059 960 528 507 503 185 184
159 45 21 16 15
BsiEI CG_ry'CG
Cuts at: 0 546 1014 3573 5727
Size: 546 468 2559 2154
Fragments arranged by size:
2559 2154 546 468
BsiHKAI G_wGCw'C
Cuts at: 0 1121 1970 2282 3283 3566 3809 4307 4628
Size: 1121 849 312 1001 283 243 498 321
Cuts at: 4628 5500 5543 5727
Size: 872 43 184
Fragments arranged by size:
1121 1001 872 849 498 321 312 283
243 184 43
BsmI GAATG_Cn'
Cuts at: 0 5289 5727
Size: 5289 438
Bsp1286I G_dGCh'C
Cuts at: 0 132 299 322 536 759 765 892 919
Size: 132 167 23 214 223 6 127 27
Cuts at: 919 928 1021 1121 1223 1727 1802 1939 1970
Size: 9 93 100 102 504 75 137 31
Cuts at: 1970 2282 2291 2462 2470 2525 2572 2645 2714
Size: 312 9 171 8 55 47 73 69
Cuts at: 2714 2812 2827 3065 3112 3189 3228 3283 3331
Size: 98 15 238 47 77 39 55 48
Cuts at: 3331 3351 3566 3712 3809 4307 4571 4628 4687
Size: 20 215 146 97 498 264 57 59
Cuts at: 4687 4859 4915 5104 5500 5543 5637 5727
Size: 172 56 189 396 43 94 90
Fragments arranged by size:
504 498 396 312 264 238 223 215
214 189 172 171 167 146 137 132
127 102 100 98 97 94 93 90
77 75 73 69 59 57 56 55
55 48 47 47 43 39 31 27
23 20 15 9 9 8 6
BspEI T'CCGG_A
Cuts at: 0 2922 5064 5727
Size: 2922 2142 663
Fragments arranged by size:
2922 2142 663
BspHI T'CATG_A
Cuts at: 0 3935 4265 5727
Size: 3935 330 1462
Fragments arranged by size:
3935 1462 330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 390 1352 1484 2053 4133 5679 5727
Size: 390 962 132 569 2080 1546 48
Fragments arranged by size:
2080 1546 962 569 390 132 48
BsrBI CCG'CTC
Cuts at: 0 725 779 5727
Size: 725 54 4948
Fragments arranged by size:
4948 725 54
BsrDI GCAATG_nn'
Cuts at: 0 1133 3696 5727
Size: 1133 2563 2031
Fragments arranged by size:
2563 2031 1133
BsrFI r'CCGG_y
Cuts at: 0 1185 2193 2530 2624 3112 3895 5153 5727
Size: 1185 1008 337 94 488 783 1258 574
Fragments arranged by size:
1258 1185 1008 783 574 488 337 94
BsrGI T'GTAC_A
Cuts at: 0 1259 3824 4421 5727
Size: 1259 2565 597 1306
Fragments arranged by size:
2565 1306 1259 597
BssHII G'CGCG_C
Cuts at: 0 4014 5727
Size: 4014 1713
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2390 3849 4611 5727
Size: 2390 1459 762 1116
Fragments arranged by size:
2390 1459 1116 762
BstEII G'GTnAC_C
Cuts at: 0 4282 4750 5727
Size: 4282 468 977
Fragments arranged by size:
4282 977 468
BstXI CCAn_nnnn'nTGG
Cuts at: 0 573 1216 2026 2863 3262 5727
Size: 573 643 810 837 399 2465
Fragments arranged by size:
2465 837 810 643 573 399
BstYI r'GATC_y
Cuts at: 0 3980 5444 5727
Size: 3980 1464 283
Fragments arranged by size:
3980 1464 283
Bsu36I CC'TnA_GG
Cuts at: 0 2540 4061 4396 5727
Size: 2540 1521 335 1331
Fragments arranged by size:
2540 1521 1331 335
BtgI C'CryG_G
Cuts at: 0 254 750 911 953 1043 1196 1275 2366
Size: 254 496 161 42 90 153 79 1091
Cuts at: 2366 3573 3752 4032 4343 4466 4875 5727
Size: 1207 179 280 311 123 409 852
Fragments arranged by size:
1207 1091 852 496 409 311 280 254
179 161 153 123 90 79 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 118 5151 5340 5727
Size: 118 5033 189 387
Fragments arranged by size:
5033 387 189 118
BtsI GCAGTG_nn'
Cuts at: 0 112 215 1365 1569 1869 1950 1968 2154
Size: 112 103 1150 204 300 81 18 186
Cuts at: 2154 2235 2388 3007 3283 3794 5727
Size: 81 153 619 276 511 1933
Fragments arranged by size:
1933 1150 619 511 300 276 204 186
153 112 103 81 81 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 101 136 5727
Size: 101 35 5591
Fragments arranged by size:
5591 101 35
DraIII CAC_nnn'GTG
Cuts at: 0 1395 1956 2076 4980 5727
Size: 1395 561 120 2904 747
Fragments arranged by size:
2904 1395 747 561 120
EaeI y'GGCC_r
Cuts at: 0 183 443 543 563 608 623 1011 1140
Size: 183 260 100 20 45 15 388 129
Cuts at: 1140 2195 2399 2436 2618 2723 2889 2991 3570
Size: 1055 204 37 182 105 166 102 579
Cuts at: 3570 4029 4080 5727
Size: 459 51 1647
Fragments arranged by size:
1647 1055 579 459 388 260 204 183
182 166 129 105 102 100 51 45
37 20 15
EagI C'GGCC_G
Cuts at: 0 543 1011 3570 5727
Size: 543 468 2559 2157
Fragments arranged by size:
2559 2157 543 468
EarI CTCTTCn'nnn_
Cuts at: 0 226 4147 4435 4798 5727
Size: 226 3921 288 363 929
Fragments arranged by size:
3921 929 363 288 226
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 604 717 733 3073 5727
Size: 604 113 16 2340 2654
Fragments arranged by size:
2654 2340 604 113 16
EcoICRI GAG'CTC
Cuts at: 0 2280 5498 5541 5727
Size: 2280 3218 43 186
Fragments arranged by size:
3218 2280 186 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1174 1192 1304 1435 1471 1760 2185
Size: 627 547 18 112 131 36 289 425
Cuts at: 2185 2195 2224 2839 2929 3175 3901 3910 3988
Size: 10 29 615 90 246 726 9 78
Cuts at: 3988 4189 4267 4960 5596 5659 5727
Size: 201 78 693 636 63 68
Fragments arranged by size:
726 693 636 627 615 547 425 289
246 201 131 112 90 78 78 68
63 36 29 18 10 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 185 865 2543 4395 4399 5641 5686 5727
Size: 185 680 1678 1852 4 1242 45 41
Fragments arranged by size:
1852 1678 1242 680 185 45 41 4
EcoO109I rG'GnC_Cy
Cuts at: 0 128 468 549 651 707 755 834 858
Size: 128 340 81 102 56 48 79 24
Cuts at: 858 1017 1018 1315 1461 1723 1724 2065 2459
Size: 159 1 297 146 262 1 341 394
Cuts at: 2459 2775 2808 2809 2948 3185 3198 3469 3525
Size: 316 33 1 139 237 13 271 56
Cuts at: 3525 3709 3846 4104 4271 4462 4567 4703 4833
Size: 184 137 258 167 191 105 136 130
Cuts at: 4833 5226 5234 5646 5727
Size: 393 8 412 81
Fragments arranged by size:
412 394 393 341 340 316 297 271
262 258 237 191 184 167 159 146
139 137 136 130 128 105 102 81
81 79 56 56 48 33 24 13
8 1 1 1
FspI TGC'GCA
Cuts at: 0 3632 5727
Size: 3632 2095
HaeII r_GCGC'y
Cuts at: 0 432 940 1196 2003 4234 4737 5727
Size: 432 508 256 807 2231 503 990
Fragments arranged by size:
2231 990 807 508 503 432 256
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 709 741 2781 2813 4765 4797 5244 5276
Size: 709 32 2040 32 1952 32 447 32
Cuts at: 5276 5727
Size: 451
Fragments arranged by size:
2040 1952 709 451 447 32 32 32
32
HincII GTy'rAC
Cuts at: 0 3300 5727
Size: 3300 2427
KasI G'GCGC_C
Cuts at: 0 428 5727
Size: 428 5299
MluI A'CGCG_T
Cuts at: 0 2109 5727
Size: 2109 3618
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 2597 4145 4646 5306 5727
Size: 707 1890 1548 501 660 421
Fragments arranged by size:
1890 1548 707 660 501 421
MscI TGG'CCA
Cuts at: 0 565 610 2620 2725 2891 2993 4031 5727
Size: 565 45 2010 105 166 102 1038 1696
Fragments arranged by size:
2010 1696 1038 565 166 105 102 45
MslI CAynn'nnrTG
Cuts at: 0 1373 2020 3820 5727
Size: 1373 647 1800 1907
Fragments arranged by size:
1907 1800 1373 647
MspA1I CmG'CkG
Cuts at: 0 354 621 752 913 1025 1257 1445 1467
Size: 354 267 131 161 112 232 188 22
Cuts at: 1467 1533 2274 2974 3132 3360 3575 3699 3738
Size: 66 741 700 158 228 215 124 39
Cuts at: 3738 3879 3977 4047 4869 5670 5727
Size: 141 98 70 822 801 57
Fragments arranged by size:
822 801 741 700 354 267 232 228
215 188 161 158 141 131 124 112
98 70 66 57 39 22
NaeI GCC'GGC
Cuts at: 0 2195 2626 5727
Size: 2195 431 3101
Fragments arranged by size:
3101 2195 431
NarI GG'CG_CC
Cuts at: 0 429 5727
Size: 429 5298
NcoI C'CATG_G
Cuts at: 0 953 2366 4032 4343 5727
Size: 953 1413 1666 311 1384
Fragments arranged by size:
1666 1413 1384 953 311
NgoMIV G'CCGG_C
Cuts at: 0 2193 2624 5727
Size: 2193 431 3103
Fragments arranged by size:
3103 2193 431
NheI G'CTAG_C
Cuts at: 0 4227 5202 5727
Size: 4227 975 525
Fragments arranged by size:
4227 975 525
NspI r_CATG'y
Cuts at: 0 4428 4717 5727
Size: 4428 289 1010
Fragments arranged by size:
4428 1010 289
PasI CC'CwG_GG
Cuts at: 0 1248 1284 2032 2304 3705 5230 5727
Size: 1248 36 748 272 1401 1525 497
Fragments arranged by size:
1525 1401 1248 748 497 272 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 384 1152 1311 1395 1878 2010 2322
Size: 42 342 768 159 84 483 132 312
Cuts at: 2322 3507 4167 4899 5574 5727
Size: 1185 660 732 675 153
Fragments arranged by size:
1185 768 732 675 660 483 342 312
159 153 132 84 42
PfoI T'CCnGG_A
Cuts at: 0 1658 2906 3506 3989 5545 5727
Size: 1658 1248 600 483 1556 182
Fragments arranged by size:
1658 1556 1248 600 483 182
PmlI CAC'GTG
Cuts at: 0 1731 5727
Size: 1731 3996
PpuMI rG'GwC_Cy
Cuts at: 0 468 549 834 1461 2065 2775 3198 4271
Size: 468 81 285 627 604 710 423 1073
Cuts at: 4271 4833 5226 5234 5646 5727
Size: 562 393 8 412 81
Fragments arranged by size:
1073 710 627 604 562 468 423 412
393 285 81 81 8
PspOMI G'GGCC_C
Cuts at: 0 128 755 761 915 1017 1723 2458 2710
Size: 128 627 6 154 102 706 735 252
Cuts at: 2710 2808 3185 3708 4567 5727
Size: 98 377 523 859 1160
Fragments arranged by size:
1160 859 735 706 627 523 377 252
154 128 102 98 6
PspXI vC'TCGA_Gb
Cuts at: 0 247 5727
Size: 247 5480
PstI C_TGCA'G
Cuts at: 0 245 1864 2386 3278 3789 4052 5111 5669
Size: 245 1619 522 892 511 263 1059 558
Cuts at: 5669 5687 5727
Size: 18 40
Fragments arranged by size:
1619 1059 892 558 522 511 263 245
40 18
PvuII CAG'CTG
Cuts at: 0 354 621 1257 1445 1533 3699 3879 4869
Size: 354 267 636 188 88 2166 180 990
Cuts at: 4869 5670 5727
Size: 801 57
Fragments arranged by size:
2166 990 801 636 354 267 188 180
88 57
SacI G_AGCT'C
Cuts at: 0 2282 5500 5543 5727
Size: 2282 3218 43 184
Fragments arranged by size:
3218 2282 184 43
SacII CC_GC'GG
Cuts at: 0 753 914 3576 5727
Size: 753 161 2662 2151
Fragments arranged by size:
2662 2151 753 161
SanDI GG'GwC_CC
Cuts at: 0 5234 5727
Size: 5234 493
SapI GCTCTTCn'nnn_
Cuts at: 0 4147 4435 5727
Size: 4147 288 1292
Fragments arranged by size:
4147 1292 288
SexAI A'CCwGG_T
Cuts at: 0 1391 1757 2072 4448 4976 5727
Size: 1391 366 315 2376 528 751
Fragments arranged by size:
2376 1391 751 528 366 315
SfcI C'TryA_G
Cuts at: 0 241 1860 1995 2382 3274 3785 4048 5107
Size: 241 1619 135 387 892 511 263 1059
Cuts at: 5107 5665 5683 5727
Size: 558 18 44
Fragments arranged by size:
1619 1059 892 558 511 387 263 241
135 44 18
SfoI GGC'GCC
Cuts at: 0 430 5727
Size: 430 5297
SmaI CCC'GGG
Cuts at: 0 506 760 887 4572 4621 5440 5727
Size: 506 254 127 3685 49 819 287
Fragments arranged by size:
3685 819 506 287 254 127 49
SmlI C'TyrA_G
Cuts at: 0 247 2751 3490 4726 5727
Size: 247 2504 739 1236 1001
Fragments arranged by size:
2504 1236 1001 739 247
SphI G_CATG'C
Cuts at: 0 4717 5727
Size: 4717 1010
SrfI GCCC'GGGC
Cuts at: 0 760 5727
Size: 760 4967
StuI AGG'CCT
Cuts at: 0 572 1876 2057 2138 2228 4742 5727
Size: 572 1304 181 81 90 2514 985
Fragments arranged by size:
2514 1304 985 572 181 90 81
StyI C'CwwG_G
Cuts at: 0 432 919 953 1877 2366 3208 4032 4343
Size: 432 487 34 924 489 842 824 311
Cuts at: 4343 4916 5009 5054 5727
Size: 573 93 45 673
Fragments arranged by size:
924 842 824 673 573 489 487 432
311 93 45 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 943 955 1261 2317 2347 2842 2875 3622
Size: 943 12 306 1056 30 495 33 747
Cuts at: 3622 3769 3964 4270 4660 5268 5727
Size: 147 195 306 390 608 459
Fragments arranged by size:
1056 943 747 608 495 459 390 306
306 195 147 33 30 12
TatI w'GTAC_w
Cuts at: 0 818 1259 3614 3824 4421 5727
Size: 818 441 2355 210 597 1306
Fragments arranged by size:
2355 1306 818 597 441 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1453 1485 2476 2508 2661 2693 2902 2934
Size: 1453 32 991 32 153 32 209 32
Cuts at: 2934 3204 3236 4835 4867 5547 5579 5727
Size: 270 32 1599 32 680 32 148
Fragments arranged by size:
1599 1453 991 680 270 209 153 148
32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1789 2779 4672 5727
Size: 1789 990 1893 1055
Fragments arranged by size:
1893 1789 1055 990
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 618 651 960 2409 2880 3444 5727
Size: 618 33 309 1449 471 564 2283
Fragments arranged by size:
2283 1449 618 564 471 309 33
XhoI C'TCGA_G
Cuts at: 0 247 5727
Size: 247 5480
XmaI C'CCGG_G
Cuts at: 0 504 758 885 4570 4619 5438 5727
Size: 504 254 127 3685 49 819 289
Fragments arranged by size:
3685 819 504 289 254 127 49
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HincII KasI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII
SacI SacII SanDI SapI SexAI SfcI SfoI SmaI
SmlI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AleI ApoI AscI AseI
AsiSI AvrII BfrBI BglII BsaBI BsiWI BsmBI BssSI
BstBI BstZ17I ClaI DraI DrdI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KpnI MfeI NdeI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PsrI PvuI RsrII SalI SbfI ScaI SfiI SgfI
SgrAI SnaBI SpeI SspI SwaI XbaI XmnI ZraI