Restriction map for
pF1KSDA1225
(Linear) (Six-base) MAPSORT of: KIAA1225.seq Check: 3431 from: 1 to: 4113
KIAA1225.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AccI GT'mk_AC
Cuts at: 0 48 4113
Size: 48 4065
Acc65I G'GTAC_C
Cuts at: 0 33 4113
Size: 33 4080
AclI AA'CG_TT
Cuts at: 0 247 4113
Size: 247 3866
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 778 1712 1814 2579 3320 3980 4113
Size: 620 158 934 102 765 741 660 133
Fragments arranged by size:
934 765 741 660 620 158 133 102
AflII C'TTAA_G
Cuts at: 0 969 1471 4113
Size: 969 502 2642
Fragments arranged by size:
2642 969 502
AflIII A'CryG_T
Cuts at: 0 751 1274 4113
Size: 751 523 2839
Fragments arranged by size:
2839 751 523
AleI CACnn'nnGTG
Cuts at: 0 592 4113
Size: 592 3521
AlwNI CAG_nnn'CTG
Cuts at: 0 1726 3253 4113
Size: 1726 1527 860
Fragments arranged by size:
1726 1527 860
ApoI r'AATT_y
Cuts at: 0 465 583 743 1143 1434 2100 3084 4113
Size: 465 118 160 400 291 666 984 1029
Fragments arranged by size:
1029 984 666 465 400 291 160 118
AseI AT'TA_AT
Cuts at: 0 275 1118 1846 2072 2393 3446 4113
Size: 275 843 728 226 321 1053 667
Fragments arranged by size:
1053 843 728 667 321 275 226
BamHI G'GATC_C
Cuts at: 0 3861 4113
Size: 3861 252
BanI G'GyrC_C
Cuts at: 0 33 1416 4113
Size: 33 1383 2697
Fragments arranged by size:
2697 1383 33
BanII G_rGCy'C
Cuts at: 0 1374 3291 4113
Size: 1374 1917 822
Fragments arranged by size:
1917 1374 822
BbsI GAAGACnn'nnnn_
Cuts at: 0 1403 1541 1606 4113
Size: 1403 138 65 2507
Fragments arranged by size:
2507 1403 138 65
BbvCI CC'TCA_GC
Cuts at: 0 2675 3020 4113
Size: 2675 345 1093
Fragments arranged by size:
2675 1093 345
BciVI GTATCCnnnnn_n'
Cuts at: 0 2447 4113
Size: 2447 1666
BclI T'GATC_A
Cuts at: 0 987 2760 4113
Size: 987 1773 1353
Fragments arranged by size:
1773 1353 987
BfrBI ATG'CAT
Cuts at: 0 3415 4113
Size: 3415 698
BglI GCCn_nnn'nGGC
Cuts at: 0 3669 4113
Size: 3669 444
BglII A'GATC_T
Cuts at: 0 2562 4113
Size: 2562 1551
BmrI ACTGGGnnnn_n'
Cuts at: 0 417 2484 4113
Size: 417 2067 1629
Fragments arranged by size:
2067 1629 417
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3348 4113
Size: 3348 765
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2030 3542 3606 4113
Size: 2030 1512 64 507
Fragments arranged by size:
2030 1512 507 64
Bpu10I CC'TnA_GC
Cuts at: 0 2675 3020 4113
Size: 2675 345 1093
Fragments arranged by size:
2675 1093 345
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 628 1090 1358 4113
Size: 469 159 462 268 2755
Fragments arranged by size:
2755 469 462 268 159
BsaI GGTCTCn'nnnn_
Cuts at: 0 58 1240 1519 3366 4113
Size: 58 1182 279 1847 747
Fragments arranged by size:
1847 1182 747 279 58
BsaBI GATnn'nnATC
Cuts at: 0 164 2843 4113
Size: 164 2679 1270
Fragments arranged by size:
2679 1270 164
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 3008 3338 4113
Size: 76 2932 330 775
Fragments arranged by size:
2932 775 330 76
BseYI C'CCAG_C
Cuts at: 0 551 3147 4113
Size: 551 2596 966
Fragments arranged by size:
2596 966 551
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2885 3368 4113
Size: 2885 483 745
Fragments arranged by size:
2885 745 483
BsiEI CG_ry'CG
Cuts at: 0 3189 4113
Size: 3189 924
BsiHKAI G_wGCw'C
Cuts at: 0 2417 3046 3435 4113
Size: 2417 629 389 678
Fragments arranged by size:
2417 678 629 389
BsmI GAATG_Cn'
Cuts at: 0 3540 4113
Size: 3540 573
Bsp1286I G_dGCh'C
Cuts at: 0 1374 2417 3046 3291 3435 4113
Size: 1374 1043 629 245 144 678
Fragments arranged by size:
1374 1043 678 629 245 144
BspHI T'CATG_A
Cuts at: 0 2166 4113
Size: 2166 1947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2763 4113
Size: 2763 1350
BsrDI GCAATG_nn'
Cuts at: 0 90 260 2767 4113
Size: 90 170 2507 1346
Fragments arranged by size:
2507 1346 170 90
BsrGI T'GTAC_A
Cuts at: 0 1503 1671 4113
Size: 1503 168 2442
Fragments arranged by size:
2442 1503 168
BssSI C'ACGA_G
Cuts at: 0 3578 4113
Size: 3578 535
BstAPI GCAn_nnn'nTGC
Cuts at: 0 262 4113
Size: 262 3851
BstYI r'GATC_y
Cuts at: 0 2562 2593 3552 3861 4113
Size: 2562 31 959 309 252
Fragments arranged by size:
2562 959 309 252 31
Bsu36I CC'TnA_GG
Cuts at: 0 3674 3707 4113
Size: 3674 33 406
Fragments arranged by size:
3674 406 33
BtsI GCAGTG_nn'
Cuts at: 0 2955 4055 4113
Size: 2955 1100 58
Fragments arranged by size:
2955 1100 58
DraI TTT'AAA
Cuts at: 0 1439 2135 4113
Size: 1439 696 1978
Fragments arranged by size:
1978 1439 696
DraIII CAC_nnn'GTG
Cuts at: 0 2972 4113
Size: 2972 1141
EarI CTCTTCn'nnn_
Cuts at: 0 53 170 1229 1517 1539 1808 2207 2852
Size: 53 117 1059 288 22 269 399 645
Cuts at: 2852 4113
Size: 1261
Fragments arranged by size:
1261 1059 645 399 288 269 117 53
22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1843 4113
Size: 1843 2270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 778 1712 1814 2030 2579 3320 3542
Size: 620 158 934 102 216 549 741 222
Cuts at: 3542 3606 3980 4113
Size: 64 374 133
Fragments arranged by size:
934 741 620 549 374 222 216 158
133 102 64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3812 4113
Size: 3812 301
EcoO109I rG'GnC_Cy
Cuts at: 0 2878 4113
Size: 2878 1235
EcoRI G'AATT_C
Cuts at: 0 583 4113
Size: 583 3530
EcoRV GAT'ATC
Cuts at: 0 1447 4113
Size: 1447 2666
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1189 1221 1912 1944 2794 2826 3259 3291
Size: 1189 32 691 32 850 32 433 32
Cuts at: 3291 3298 3330 4113
Size: 7 32 783
Fragments arranged by size:
1189 850 783 691 433 32 32 32
32 32 7
HincII GTy'rAC
Cuts at: 0 1767 2244 2535 4113
Size: 1767 477 291 1578
Fragments arranged by size:
1767 1578 477 291
HindIII A'AGCT_T
Cuts at: 0 935 2379 4113
Size: 935 1444 1734
Fragments arranged by size:
1734 1444 935
HpaI GTT'AAC
Cuts at: 0 1767 4113
Size: 1767 2346
KpnI G_GTAC'C
Cuts at: 0 37 4113
Size: 37 4076
MfeI C'AATT_G
Cuts at: 0 353 1174 1968 4113
Size: 353 821 794 2145
Fragments arranged by size:
2145 821 794 353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1474 2571 4113
Size: 1474 1097 1542
Fragments arranged by size:
1542 1474 1097
MslI CAynn'nnrTG
Cuts at: 0 592 1386 1775 2519 3101 4113
Size: 592 794 389 744 582 1012
Fragments arranged by size:
1012 794 744 592 582 389
MspA1I CmG'CkG
Cuts at: 0 408 555 1368 2260 3337 4113
Size: 408 147 813 892 1077 776
Fragments arranged by size:
1077 892 813 776 408 147
NsiI A_TGCA'T
Cuts at: 0 3417 4113
Size: 3417 696
NspI r_CATG'y
Cuts at: 0 755 1278 4113
Size: 755 523 2835
Fragments arranged by size:
2835 755 523
PasI CC'CwG_GG
Cuts at: 0 1424 4113
Size: 1424 2689
PciI A'CATG_T
Cuts at: 0 751 1274 4113
Size: 751 523 2839
Fragments arranged by size:
2839 751 523
PflMI CCAn_nnn'nTGG
Cuts at: 0 1021 1257 3825 3871 4113
Size: 1021 236 2568 46 242
Fragments arranged by size:
2568 1021 242 236 46
PfoI T'CCnGG_A
Cuts at: 0 3558 4113
Size: 3558 555
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2140 2172 4113
Size: 2140 32 1941
Fragments arranged by size:
2140 1941 32
PsiI TTA'TAA
Cuts at: 0 2311 4027 4113
Size: 2311 1716 86
Fragments arranged by size:
2311 1716 86
PstI C_TGCA'G
Cuts at: 0 2958 3204 3986 4113
Size: 2958 246 782 127
Fragments arranged by size:
2958 782 246 127
PvuII CAG'CTG
Cuts at: 0 408 555 2260 4113
Size: 408 147 1705 1853
Fragments arranged by size:
1853 1705 408 147
SapI GCTCTTCn'nnn_
Cuts at: 0 170 2207 2852 4113
Size: 170 2037 645 1261
Fragments arranged by size:
2037 1261 645 170
ScaI AGT'ACT
Cuts at: 0 605 1614 3599 4113
Size: 605 1009 1985 514
Fragments arranged by size:
1985 1009 605 514
SfcI C'TryA_G
Cuts at: 0 881 1168 1619 2954 3200 3982 4017 4113
Size: 881 287 451 1335 246 782 35 96
Fragments arranged by size:
1335 881 782 451 287 246 96 35
SmlI C'TyrA_G
Cuts at: 0 448 607 969 1069 1373 1471 4113
Size: 448 159 362 100 304 98 2642
Fragments arranged by size:
2642 448 362 304 159 100 98
SpeI A'CTAG_T
Cuts at: 0 3499 4113
Size: 3499 614
SspI AAT'ATT
Cuts at: 0 494 723 4032 4113
Size: 494 229 3309 81
Fragments arranged by size:
3309 494 229 81
StuI AGG'CCT
Cuts at: 0 3672 4113
Size: 3672 441
StyI C'CwwG_G
Cuts at: 0 140 1291 4113
Size: 140 1151 2822
Fragments arranged by size:
2822 1151 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1081 1582 3203 4113
Size: 1081 501 1621 910
Fragments arranged by size:
1621 1081 910 501
TatI w'GTAC_w
Cuts at: 0 603 1503 1612 1671 2686 3214 3597 4113
Size: 603 900 109 59 1015 528 383 516
Fragments arranged by size:
1015 900 603 528 516 383 109 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2585 2617 4034 4066 4113
Size: 2585 32 1417 32 47
Fragments arranged by size:
2585 1417 47 32 32
XbaI T'CTAG_A
Cuts at: 0 3733 4113
Size: 3733 380
XmnI GAAnn'nnTTC
Cuts at: 0 107 179 761 4113
Size: 107 72 582 3352
Fragments arranged by size:
3352 582 107 72
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlwNI
ApoI AseI BamHI BanI BanII BbsI BbvCI BciVI
BclI BfrBI BglI BglII BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstAPI
BstYI Bsu36I BtsI DraI DraIII EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV Hin4I HincII HindIII HpaI
KpnI MfeI MmeI MslI MspA1I NsiI NspI PasI
PciI PflMI PfoI PpiI PsiI PstI PvuII SapI
ScaI SfcI SmlI SpeI SspI StuI StyI TaqII
TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AlfI AloI ApaI
ApaLI AscI AsiSI AvaI AvrII BaeI BbeI BcgI
BlpI Bme1580I BmgBI BmtI BsaAI BsaHI BsaWI BsaXI
BsiWI BsmBI BspEI BsrBI BsrFI BssHII BstBI BstEII
BstXI BstZ17I BtgI BtgZI ClaI CspCI DrdI EaeI
EagI EcoICRI FalI FseI FspI FspAI HaeII KasI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PmeI PmlI PpuMI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SwaI Tth111I XcmI XhoI XmaI ZraI