Restriction map for
pF1KSDA1227
(Linear) (Six-base) MAPSORT of: KIAA1227.seq Check: 446 from: 1 to: 3198
KIAA1227.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2305 2534 2678 2873 3198
Size: 2305 229 144 195 325
Fragments arranged by size:
2305 325 229 195 144
AflII C'TTAA_G
Cuts at: 0 1654 3198
Size: 1654 1544
AflIII A'CryG_T
Cuts at: 0 382 567 1697 2534 2819 2876 3198
Size: 382 185 1130 837 285 57 322
Fragments arranged by size:
1130 837 382 322 285 185 57
AhdI GACnn_n'nnGTC
Cuts at: 0 1778 3198
Size: 1778 1420
AleI CACnn'nnGTG
Cuts at: 0 2450 3198
Size: 2450 748
AlwNI CAG_nnn'CTG
Cuts at: 0 1366 1579 2369 2994 3198
Size: 1366 213 790 625 204
Fragments arranged by size:
1366 790 625 213 204
ApoI r'AATT_y
Cuts at: 0 1597 1633 1849 3198
Size: 1597 36 216 1349
Fragments arranged by size:
1597 1349 216 36
AvaI C'yCGr_G
Cuts at: 0 1991 2278 2332 2671 3198
Size: 1991 287 54 339 527
Fragments arranged by size:
1991 527 339 287 54
BanI G'GyrC_C
Cuts at: 0 2375 2409 2667 3198
Size: 2375 34 258 531
Fragments arranged by size:
2375 531 258 34
BanII G_rGCy'C
Cuts at: 0 129 1298 2495 3198
Size: 129 1169 1197 703
Fragments arranged by size:
1197 1169 703 129
BbsI GAAGACnn'nnnn_
Cuts at: 0 1672 2581 2603 3198
Size: 1672 909 22 595
Fragments arranged by size:
1672 909 595 22
BclI T'GATC_A
Cuts at: 0 1904 3198
Size: 1904 1294
BfrBI ATG'CAT
Cuts at: 0 2073 3198
Size: 2073 1125
BglI GCCn_nnn'nGGC
Cuts at: 0 3014 3198
Size: 3014 184
BglII A'GATC_T
Cuts at: 0 1658 1987 3198
Size: 1658 329 1211
Fragments arranged by size:
1658 1211 329
BlpI GC'TnA_GC
Cuts at: 0 2186 3198
Size: 2186 1012
Bme1580I G_kGCm'C
Cuts at: 0 57 2926 3062 3198
Size: 57 2869 136 136
Fragments arranged by size:
2869 136 136 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1557 3198
Size: 1557 1641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2791 2877 3016 3020 3198
Size: 2791 86 139 4 178
Fragments arranged by size:
2791 178 139 86 4
Bpu10I CC'TnA_GC
Cuts at: 0 2489 3198
Size: 2489 709
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 679 1181 1527 2004 3198
Size: 350 329 502 346 477 1194
Fragments arranged by size:
1194 502 477 350 346 329
BsaI GGTCTCn'nnnn_
Cuts at: 0 104 813 1385 1514 1614 3198
Size: 104 709 572 129 100 1584
Fragments arranged by size:
1584 709 572 129 104 100
BsaAI yAC'GTr
Cuts at: 0 2820 3198
Size: 2820 378
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2776 2806 2925 2955 3198
Size: 2776 30 119 30 243
Fragments arranged by size:
2776 243 119 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 1006 2120 2653 2665 3198
Size: 79 927 1114 533 12 533
Fragments arranged by size:
1114 927 533 533 79 12
BseYI C'CCAG_C
Cuts at: 0 1297 1440 2483 3198
Size: 1297 143 1043 715
Fragments arranged by size:
1297 1043 715 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 3198
Size: 14 3184
BsiEI CG_ry'CG
Cuts at: 0 2243 3198
Size: 2243 955
BsiHKAI G_wGCw'C
Cuts at: 0 129 1505 2797 3198
Size: 129 1376 1292 401
Fragments arranged by size:
1376 1292 401 129
BsmI GAATG_Cn'
Cuts at: 0 663 3198
Size: 663 2535
BsmBI CGTCTCn'nnnn_
Cuts at: 0 76 3198
Size: 76 3122
Bsp1286I G_dGCh'C
Cuts at: 0 57 129 1298 1505 2495 2797 2926 3062
Size: 57 72 1169 207 990 302 129 136
Cuts at: 3062 3198
Size: 136
Fragments arranged by size:
1169 990 302 207 136 136 129 72
57
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1666 2335 3198
Size: 1666 669 863
Fragments arranged by size:
1666 863 669
BsrBI CCG'CTC
Cuts at: 0 2223 3198
Size: 2223 975
BsrDI GCAATG_nn'
Cuts at: 0 536 1647 3198
Size: 536 1111 1551
Fragments arranged by size:
1551 1111 536
BssSI C'ACGA_G
Cuts at: 0 2218 2296 3198
Size: 2218 78 902
Fragments arranged by size:
2218 902 78
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1649 3198
Size: 1649 1549
BstYI r'GATC_y
Cuts at: 0 670 961 1658 1987 2984 3198
Size: 670 291 697 329 997 214
Fragments arranged by size:
997 697 670 329 291 214
Bsu36I CC'TnA_GG
Cuts at: 0 1219 2263 3198
Size: 1219 1044 935
Fragments arranged by size:
1219 1044 935
BtgI C'CryG_G
Cuts at: 0 823 3198
Size: 823 2375
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1198 3198
Size: 1198 2000
BtsI GCAGTG_nn'
Cuts at: 0 722 2974 3198
Size: 722 2252 224
Fragments arranged by size:
2252 722 224
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 818 853 3198
Size: 818 35 2345
Fragments arranged by size:
2345 818 35
DraI TTT'AAA
Cuts at: 0 251 1157 1638 3117 3198
Size: 251 906 481 1479 81
Fragments arranged by size:
1479 906 481 251 81
DrdI GACnn_nn'nnGTC
Cuts at: 0 2858 3198
Size: 2858 340
EaeI y'GGCC_r
Cuts at: 0 622 826 2240 3198
Size: 622 204 1414 958
Fragments arranged by size:
1414 958 622 204
EagI C'GGCC_G
Cuts at: 0 2240 3198
Size: 2240 958
EarI CTCTTCn'nnn_
Cuts at: 0 280 811 2199 2657 3198
Size: 280 531 1388 458 541
Fragments arranged by size:
1388 541 531 458 280
EcoICRI GAG'CTC
Cuts at: 0 127 3198
Size: 127 3071
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2305 2534 2678 2791 2873 2877 3016 3020
Size: 2305 229 144 113 82 4 139 4
Cuts at: 3020 3198
Size: 178
Fragments arranged by size:
2305 229 178 144 139 113 82 4
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 61 3198
Size: 61 3137
EcoO109I rG'GnC_Cy
Cuts at: 0 1223 1899 2474 2701 3198
Size: 1223 676 575 227 497
Fragments arranged by size:
1223 676 575 497 227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 533 957 1998 3198
Size: 533 424 1041 1200
Fragments arranged by size:
1200 1041 533 424
FspI TGC'GCA
Cuts at: 0 1282 2344 3198
Size: 1282 1062 854
Fragments arranged by size:
1282 1062 854
FspAI rTGC'GCAy
Cuts at: 0 1282 2344 3198
Size: 1282 1062 854
Fragments arranged by size:
1282 1062 854
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 852 884 1063 1095 1492 1524 1764 1796
Size: 852 32 179 32 397 32 240 32
Cuts at: 1796 2976 3008 3198
Size: 1180 32 190
Fragments arranged by size:
1180 852 397 240 190 179 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1244 1695 2562 3198
Size: 1244 451 867 636
Fragments arranged by size:
1244 867 636 451
HindIII A'AGCT_T
Cuts at: 0 2831 3198
Size: 2831 367
HpaI GTT'AAC
Cuts at: 0 1695 2562 3198
Size: 1695 867 636
Fragments arranged by size:
1695 867 636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 403 1292 1589 3198
Size: 87 316 889 297 1609
Fragments arranged by size:
1609 889 316 297 87
MslI CAynn'nnrTG
Cuts at: 0 1688 2450 3198
Size: 1688 762 748
Fragments arranged by size:
1688 762 748
MspA1I CmG'CkG
Cuts at: 0 84 1308 1366 2769 3198
Size: 84 1224 58 1403 429
Fragments arranged by size:
1403 1224 429 84 58
NsiI A_TGCA'T
Cuts at: 0 2075 3198
Size: 2075 1123
NspI r_CATG'y
Cuts at: 0 571 1701 1834 2128 2343 2880 3198
Size: 571 1130 133 294 215 537 318
Fragments arranged by size:
1130 571 537 318 294 215 133
PasI CC'CwG_GG
Cuts at: 0 1086 3198
Size: 1086 2112
PciI A'CATG_T
Cuts at: 0 567 1697 2876 3198
Size: 567 1130 1179 322
Fragments arranged by size:
1179 1130 567 322
PflMI CCAn_nnn'nTGG
Cuts at: 0 958 2723 2994 3198
Size: 958 1765 271 204
Fragments arranged by size:
1765 958 271 204
PfoI T'CCnGG_A
Cuts at: 0 2076 3198
Size: 2076 1122
PmlI CAC'GTG
Cuts at: 0 2820 3198
Size: 2820 378
PpuMI rG'GwC_Cy
Cuts at: 0 1223 2701 3198
Size: 1223 1478 497
Fragments arranged by size:
1478 1223 497
PshAI GACnn'nnGTC
Cuts at: 0 1736 2603 3198
Size: 1736 867 595
Fragments arranged by size:
1736 867 595
PspXI vC'TCGA_Gb
Cuts at: 0 2332 3198
Size: 2332 866
PstI C_TGCA'G
Cuts at: 0 2260 3198
Size: 2260 938
PvuII CAG'CTG
Cuts at: 0 84 1366 2769 3198
Size: 84 1282 1403 429
Fragments arranged by size:
1403 1282 429 84
RsrII CG'GwC_CG
Cuts at: 0 1464 3198
Size: 1464 1734
SacI G_AGCT'C
Cuts at: 0 129 3198
Size: 129 3069
SapI GCTCTTCn'nnn_
Cuts at: 0 280 3198
Size: 280 2918
SexAI A'CCwGG_T
Cuts at: 0 765 2378 3198
Size: 765 1613 820
Fragments arranged by size:
1613 820 765
SfcI C'TryA_G
Cuts at: 0 318 2256 3198
Size: 318 1938 942
Fragments arranged by size:
1938 942 318
SmlI C'TyrA_G
Cuts at: 0 365 658 1196 1542 1654 1983 1991 2332
Size: 365 293 538 346 112 329 8 341
Cuts at: 2332 3198
Size: 866
Fragments arranged by size:
866 538 365 346 341 329 293 112
8
SphI G_CATG'C
Cuts at: 0 2128 2343 3198
Size: 2128 215 855
Fragments arranged by size:
2128 855 215
StuI AGG'CCT
Cuts at: 0 1194 3017 3198
Size: 1194 1823 181
Fragments arranged by size:
1823 1194 181
StyI C'CwwG_G
Cuts at: 0 2091 3198
Size: 2091 1107
SwaI ATTT'AAAT
Cuts at: 0 1638 3198
Size: 1638 1560
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1371 1481 2427 3198
Size: 1371 110 946 771
Fragments arranged by size:
1371 946 771 110
TatI w'GTAC_w
Cuts at: 0 923 2014 3198
Size: 923 1091 1184
Fragments arranged by size:
1184 1091 923
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2776 2808 2925 2957 3198
Size: 2776 32 117 32 241
Fragments arranged by size:
2776 241 117 32 32
XbaI T'CTAG_A
Cuts at: 0 801 3198
Size: 801 2397
XhoI C'TCGA_G
Cuts at: 0 1991 2332 3198
Size: 1991 341 866
Fragments arranged by size:
1991 866 341
XmnI GAAnn'nnTTC
Cuts at: 0 161 590 2749 3198
Size: 161 429 2159 449
Fragments arranged by size:
2159 449 429 161
Enzymes that do cut:
AcuI AflII AflIII AhdI AleI AlwNI ApoI AvaI
BanI BanII BbsI BclI BfrBI BglI BglII BlpI
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BssSI BstAPI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI Hin4I
HincII HindIII HpaI MmeI MslI MspA1I NsiI NspI
PasI PciI PflMI PfoI PmlI PpuMI PshAI PspXI
PstI PvuII RsrII SacI SapI SexAI SfcI SmlI
SphI StuI StyI SwaI TaqII TatI TstI XbaI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BmgBI BmrI BmtI
BsaBI BsaHI BsaWI BsiWI BspEI BspHI BsrFI BsrGI
BssHII BstBI BstEII BstXI BstZ17I ClaI DraIII EciI
EcoRI EcoRV FseI HaeII KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PmeI PpiI PsiI PspOMI PsrI PvuI
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI Tth111I XcmI
XmaI ZraI