Restriction map for
pF1KA1231
(Linear) (Six-base) MAPSORT of: KIAA1231.seq Check: 6795 from: 1 to: 3183
KIAA1231.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1196 1600 3075 3183
Size: 1196 404 1475 108
Fragments arranged by size:
1475 1196 404 108
AccI GT'mk_AC
Cuts at: 0 948 3183
Size: 948 2235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 724 1279 2137 3183
Size: 275 449 555 858 1046
Fragments arranged by size:
1046 858 555 449 275
AflIII A'CryG_T
Cuts at: 0 1641 2837 3183
Size: 1641 1196 346
Fragments arranged by size:
1641 1196 346
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3050 3181 3183
Size: 3050 131 2
Fragments arranged by size:
3050 131 2
AlwNI CAG_nnn'CTG
Cuts at: 0 63 633 702 1302 2793 3183
Size: 63 570 69 600 1491 390
Fragments arranged by size:
1491 600 570 390 69 63
ApaLI G'TGCA_C
Cuts at: 0 1677 3183
Size: 1677 1506
ApoI r'AATT_y
Cuts at: 0 749 970 2510 2820 2859 3183
Size: 749 221 1540 310 39 324
Fragments arranged by size:
1540 749 324 310 221 39
AvaI C'yCGr_G
Cuts at: 0 347 3183
Size: 347 2836
BanI G'GyrC_C
Cuts at: 0 1215 2013 2221 3183
Size: 1215 798 208 962
Fragments arranged by size:
1215 962 798 208
BanII G_rGCy'C
Cuts at: 0 472 692 2141 2747 3183
Size: 472 220 1449 606 436
Fragments arranged by size:
1449 606 472 436 220
BbsI GAAGACnn'nnnn_
Cuts at: 0 197 554 1142 1504 3183
Size: 197 357 588 362 1679
Fragments arranged by size:
1679 588 362 357 197
BbvCI CC'TCA_GC
Cuts at: 0 1127 2558 2654 2721 3183
Size: 1127 1431 96 67 462
Fragments arranged by size:
1431 1127 462 96 67
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2415 2449 3049 3083 3183
Size: 2415 34 600 34 100
Fragments arranged by size:
2415 600 100 34 34
BclI T'GATC_A
Cuts at: 0 2366 3183
Size: 2366 817
BfrBI ATG'CAT
Cuts at: 0 1824 3183
Size: 1824 1359
BglI GCCn_nnn'nGGC
Cuts at: 0 2731 3183
Size: 2731 452
BglII A'GATC_T
Cuts at: 0 1667 3183
Size: 1667 1516
BlpI GC'TnA_GC
Cuts at: 0 2197 2735 2746 3183
Size: 2197 538 11 437
Fragments arranged by size:
2197 538 437 11
Bme1580I G_kGCm'C
Cuts at: 0 1220 1681 2224 3183
Size: 1220 461 543 959
Fragments arranged by size:
1220 959 543 461
BmgBI CAC'GTC
Cuts at: 0 1895 3183
Size: 1895 1288
BmrI ACTGGGnnnn_n'
Cuts at: 0 228 429 1140 2843 3183
Size: 228 201 711 1703 340
Fragments arranged by size:
1703 711 340 228 201
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2155 3183
Size: 2155 1028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 643 2198 2429 2533 2930 3183
Size: 151 492 1555 231 104 397 253
Fragments arranged by size:
1555 492 397 253 231 151 104
Bpu10I CC'TnA_GC
Cuts at: 0 1127 2558 2654 2721 3183
Size: 1127 1431 96 67 462
Fragments arranged by size:
1431 1127 462 96 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 3183
Size: 484 2699
BsaAI yAC'GTr
Cuts at: 0 1644 2840 3183
Size: 1644 1196 343
Fragments arranged by size:
1644 1196 343
BsaHI Gr'CG_yC
Cuts at: 0 960 2539 3183
Size: 960 1579 644
Fragments arranged by size:
1579 960 644
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1706 1736 2402 2432 2889 2919 3041 3064
Size: 1706 30 666 30 457 30 122 23
Cuts at: 3064 3071 3094 3183
Size: 7 23 89
Fragments arranged by size:
1706 666 457 122 89 30 30 30
23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 3183
Size: 283 2900
BseYI C'CCAG_C
Cuts at: 0 2210 2324 2379 2666 2726 3183
Size: 2210 114 55 287 60 457
Fragments arranged by size:
2210 457 287 114 60 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1168 2704 2851 3053 3183
Size: 1168 1536 147 202 130
Fragments arranged by size:
1536 1168 202 147 130
BsiEI CG_ry'CG
Cuts at: 0 337 955 1857 3183
Size: 337 618 902 1326
Fragments arranged by size:
1326 902 618 337
BsiHKAI G_wGCw'C
Cuts at: 0 344 377 1231 1681 2141 2203 3183
Size: 344 33 854 450 460 62 980
Fragments arranged by size:
980 854 460 450 344 62 33
BsmI GAATG_Cn'
Cuts at: 0 1290 1737 2150 3183
Size: 1290 447 413 1033
Fragments arranged by size:
1290 1033 447 413
BsmBI CGTCTCn'nnnn_
Cuts at: 0 568 3183
Size: 568 2615
Bsp1286I G_dGCh'C
Cuts at: 0 344 377 472 692 1220 1231 1681 2141
Size: 344 33 95 220 528 11 450 460
Cuts at: 2141 2203 2224 2747 3183
Size: 62 21 523 436
Fragments arranged by size:
528 523 460 450 436 344 220 95
62 33 21 11
BspHI T'CATG_A
Cuts at: 0 753 870 3183
Size: 753 117 2313
Fragments arranged by size:
2313 753 117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1196 1600 2441 2852 3075 3183
Size: 1196 404 841 411 223 108
Fragments arranged by size:
1196 841 411 404 223 108
BsrBI CCG'CTC
Cuts at: 0 1868 3183
Size: 1868 1315
BsrDI GCAATG_nn'
Cuts at: 0 40 2453 3183
Size: 40 2413 730
Fragments arranged by size:
2413 730 40
BsrFI r'CCGG_y
Cuts at: 0 332 336 3183
Size: 332 4 2847
Fragments arranged by size:
2847 332 4
BstAPI GCAn_nnn'nTGC
Cuts at: 0 727 1517 1821 2793 3183
Size: 727 790 304 972 390
Fragments arranged by size:
972 790 727 390 304
BstBI TT'CG_AA
Cuts at: 0 2301 3183
Size: 2301 882
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2701 3183
Size: 2701 482
BstYI r'GATC_y
Cuts at: 0 207 1667 3183
Size: 207 1460 1516
Fragments arranged by size:
1516 1460 207
BtgI C'CryG_G
Cuts at: 0 446 1395 2868 3183
Size: 446 949 1473 315
Fragments arranged by size:
1473 949 446 315
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2211 3183
Size: 2211 972
BtsI GCAGTG_nn'
Cuts at: 0 1348 2253 3183
Size: 1348 905 930
Fragments arranged by size:
1348 930 905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 239 274 3183
Size: 239 35 2909
Fragments arranged by size:
2909 239 35
DraI TTT'AAA
Cuts at: 0 1332 3183
Size: 1332 1851
DraIII CAC_nnn'GTG
Cuts at: 0 660 1685 3076 3183
Size: 660 1025 1391 107
Fragments arranged by size:
1391 1025 660 107
DrdI GACnn_nn'nnGTC
Cuts at: 0 2171 2457 3183
Size: 2171 286 726
Fragments arranged by size:
2171 726 286
EaeI y'GGCC_r
Cuts at: 0 334 604 651 952 1562 2594 2637 2958
Size: 334 270 47 301 610 1032 43 321
Cuts at: 2958 3183
Size: 225
Fragments arranged by size:
1032 610 334 321 301 270 225 47
43
EagI C'GGCC_G
Cuts at: 0 334 952 3183
Size: 334 618 2231
Fragments arranged by size:
2231 618 334
EarI CTCTTCn'nnn_
Cuts at: 0 140 1199 1298 1978 2003 3183
Size: 140 1059 99 680 25 1180
Fragments arranged by size:
1180 1059 680 140 99 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 967 3183
Size: 967 2216
EcoICRI GAG'CTC
Cuts at: 0 2139 3183
Size: 2139 1044
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 275 643 724 1279 2137 2198 2429
Size: 151 124 368 81 555 858 61 231
Cuts at: 2429 2533 2930 3183
Size: 104 397 253
Fragments arranged by size:
858 555 397 368 253 231 151 124
104 81 61
EcoO109I rG'GnC_Cy
Cuts at: 0 454 3183
Size: 454 2729
EcoRI G'AATT_C
Cuts at: 0 2510 2820 2859 3183
Size: 2510 310 39 324
Fragments arranged by size:
2510 324 310 39
FspI TGC'GCA
Cuts at: 0 1252 1648 3183
Size: 1252 396 1535
Fragments arranged by size:
1535 1252 396
HaeII r_GCGC'y
Cuts at: 0 2405 3183
Size: 2405 778
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1413 1445 1812 1844 2889 2921 3041 3073
Size: 1413 32 367 32 1045 32 120 32
Cuts at: 3073 3183
Size: 110
Fragments arranged by size:
1413 1045 367 120 110 32 32 32
32
HincII GTy'rAC
Cuts at: 0 949 2024 3183
Size: 949 1075 1159
Fragments arranged by size:
1159 1075 949
HpaI GTT'AAC
Cuts at: 0 2024 3183
Size: 2024 1159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 2187 2357 3183
Size: 116 2071 170 826
Fragments arranged by size:
2071 826 170 116
MscI TGG'CCA
Cuts at: 0 653 1564 2639 2960 3183
Size: 653 911 1075 321 223
Fragments arranged by size:
1075 911 653 321 223
MslI CAynn'nnrTG
Cuts at: 0 1574 2524 2680 3183
Size: 1574 950 156 503
Fragments arranged by size:
1574 950 503 156
MspA1I CmG'CkG
Cuts at: 0 1131 2170 2214 2362 2383 2571 3183
Size: 1131 1039 44 148 21 188 612
Fragments arranged by size:
1131 1039 612 188 148 44 21
NcoI C'CATG_G
Cuts at: 0 1395 3183
Size: 1395 1788
NsiI A_TGCA'T
Cuts at: 0 1826 3183
Size: 1826 1357
NspI r_CATG'y
Cuts at: 0 289 1251 1731 1815 1965 3183
Size: 289 962 480 84 150 1218
Fragments arranged by size:
1218 962 480 289 150 84
PflMI CCAn_nnn'nTGG
Cuts at: 0 2855 3183
Size: 2855 328
PfoI T'CCnGG_A
Cuts at: 0 1358 2175 3183
Size: 1358 817 1008
Fragments arranged by size:
1358 1008 817
PmlI CAC'GTG
Cuts at: 0 1644 3183
Size: 1644 1539
PsiI TTA'TAA
Cuts at: 0 835 2084 3183
Size: 835 1249 1099
Fragments arranged by size:
1249 1099 835
PstI C_TGCA'G
Cuts at: 0 113 1193 1283 2798 3183
Size: 113 1080 90 1515 385
Fragments arranged by size:
1515 1080 385 113 90
PvuII CAG'CTG
Cuts at: 0 1131 2214 2362 2571 3183
Size: 1131 1083 148 209 612
Fragments arranged by size:
1131 1083 612 209 148
RsrII CG'GwC_CG
Cuts at: 0 1743 3183
Size: 1743 1440
SacI G_AGCT'C
Cuts at: 0 2141 3183
Size: 2141 1042
SalI G'TCGA_C
Cuts at: 0 947 3183
Size: 947 2236
SapI GCTCTTCn'nnn_
Cuts at: 0 1298 3183
Size: 1298 1885
SexAI A'CCwGG_T
Cuts at: 0 56 632 3183
Size: 56 576 2551
Fragments arranged by size:
2551 576 56
SfcI C'TryA_G
Cuts at: 0 109 1189 1279 2393 2794 3183
Size: 109 1080 90 1114 401 389
Fragments arranged by size:
1114 1080 401 389 109 90
SmaI CCC'GGG
Cuts at: 0 349 3183
Size: 349 2834
SmlI C'TyrA_G
Cuts at: 0 499 3183
Size: 499 2684
SphI G_CATG'C
Cuts at: 0 289 1965 3183
Size: 289 1676 1218
Fragments arranged by size:
1676 1218 289
StuI AGG'CCT
Cuts at: 0 37 1354 1696 3183
Size: 37 1317 342 1487
Fragments arranged by size:
1487 1317 342 37
StyI C'CwwG_G
Cuts at: 0 1395 1463 3183
Size: 1395 68 1720
Fragments arranged by size:
1720 1395 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 448 928 1237 2006 2141 2224 2455 3183
Size: 448 480 309 769 135 83 231 728
Fragments arranged by size:
769 728 480 448 309 231 135 83
TatI w'GTAC_w
Cuts at: 0 2267 2516 3122 3183
Size: 2267 249 606 61
Fragments arranged by size:
2267 606 249 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2402 2434 3064 3096 3183
Size: 2402 32 630 32 87
Fragments arranged by size:
2402 630 87 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 257 681 3183
Size: 257 424 2502
Fragments arranged by size:
2502 424 257
XmaI C'CCGG_G
Cuts at: 0 347 3183
Size: 347 2836
XmnI GAAnn'nnTTC
Cuts at: 0 1318 1354 1612 2863 3183
Size: 1318 36 258 1251 320
Fragments arranged by size:
1318 1251 320 258 36
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApaLI ApoI
AvaI BanI BanII BbsI BbvCI BcgI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HpaI MmeI MscI MslI MspA1I NcoI NsiI
NspI PflMI PfoI PmlI PsiI PstI PvuII RsrII
SacI SalI SapI SexAI SfcI SmaI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BciVI BmtI BsaI BsaBI BsaWI BsiWI BspEI
BsrGI BssHII BssSI BstEII BstZ17I Bsu36I ClaI EcoNI
EcoRV FalI FseI FspAI HindIII KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PmeI PpiI PpuMI PshAI PspOMI
PspXI PsrI PvuI SacII SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XhoI ZraI