Restriction map for
pF1KSDA1234
(Linear) (Six-base) MAPSORT of: KIAA1234.seq Check: 1213 from: 1 to: 2157
KIAA1234.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2008 2157
Size: 2008 149
AleI CACnn'nnGTG
Cuts at: 0 2055 2157
Size: 2055 102
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1483 2157
Size: 1483 674
AlwNI CAG_nnn'CTG
Cuts at: 0 331 2157
Size: 331 1826
ApaI G_GGCC'C
Cuts at: 0 618 1119 2157
Size: 618 501 1038
Fragments arranged by size:
1038 618 501
ApaLI G'TGCA_C
Cuts at: 0 30 1492 1970 2142 2157
Size: 30 1462 478 172 15
Fragments arranged by size:
1462 478 172 30 15
ApoI r'AATT_y
Cuts at: 0 799 2157
Size: 799 1358
AvaI C'yCGr_G
Cuts at: 0 708 722 760 2086 2157
Size: 708 14 38 1326 71
Fragments arranged by size:
1326 708 71 38 14
BanI G'GyrC_C
Cuts at: 0 292 825 876 1297 2082 2157
Size: 292 533 51 421 785 75
Fragments arranged by size:
785 533 421 292 75 51
BanII G_rGCy'C
Cuts at: 0 274 618 1119 1174 1429 1557 1848 1928
Size: 274 344 501 55 255 128 291 80
Cuts at: 1928 2042 2157
Size: 114 115
Fragments arranged by size:
501 344 291 274 255 128 115 114
80 55
BbeI G_GCGC'C
Cuts at: 0 296 829 2157
Size: 296 533 1328
Fragments arranged by size:
1328 533 296
BbvCI CC'TCA_GC
Cuts at: 0 1544 2012 2157
Size: 1544 468 145
Fragments arranged by size:
1544 468 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1629 1663 2157
Size: 1629 34 494
Fragments arranged by size:
1629 494 34
BfrBI ATG'CAT
Cuts at: 0 415 2157
Size: 415 1742
BglI GCCn_nnn'nGGC
Cuts at: 0 88 2157
Size: 88 2069
BlpI GC'TnA_GC
Cuts at: 0 1207 1556 1847 2157
Size: 1207 349 291 310
Fragments arranged by size:
1207 349 310 291
Bme1580I G_kGCm'C
Cuts at: 0 34 618 631 1101 1119 1393 1496 1715
Size: 34 584 13 470 18 274 103 219
Cuts at: 1715 1832 1974 2087 2146 2157
Size: 117 142 113 59 11
Fragments arranged by size:
584 470 274 219 142 117 113 103
59 34 18 13 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 537 1430 1938 2049 2157
Size: 537 893 508 111 108
Fragments arranged by size:
893 537 508 111 108
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 288 859 1259 1695 1812 2157
Size: 288 571 400 436 117 345
Fragments arranged by size:
571 436 400 345 288 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 1229 1241 1431 1571 1942 2157
Size: 361 868 12 190 140 371 215
Fragments arranged by size:
868 371 361 215 190 140 12
Bpu10I CC'TnA_GC
Cuts at: 0 610 1069 1173 1544 2012 2157
Size: 610 459 104 371 468 145
Fragments arranged by size:
610 468 459 371 145 104
BsaAI yAC'GTr
Cuts at: 0 35 492 2157
Size: 35 457 1665
Fragments arranged by size:
1665 457 35
BsaHI Gr'CG_yC
Cuts at: 0 293 826 2157
Size: 293 533 1331
Fragments arranged by size:
1331 533 293
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 169 199 869 899 1321 1351
Size: 19 30 120 30 670 30 422 30
Cuts at: 1351 2157
Size: 806
Fragments arranged by size:
806 670 422 120 30 30 30 30
19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 69 877 930 1201 1204 1536 2157
Size: 69 808 53 271 3 332 621
Fragments arranged by size:
808 621 332 271 69 53 3
BseYI C'CCAG_C
Cuts at: 0 1318 1714 1728 1752 1870 2157
Size: 1318 396 14 24 118 287
Fragments arranged by size:
1318 396 287 118 24 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 413 1889 1980 2157
Size: 280 133 1476 91 177
Fragments arranged by size:
1476 280 177 133 91
BsiEI CG_ry'CG
Cuts at: 0 290 428 501 2157
Size: 290 138 73 1656
Fragments arranged by size:
1656 290 138 73
BsiHKAI G_wGCw'C
Cuts at: 0 34 274 1070 1496 1557 1974 2146 2157
Size: 34 240 796 426 61 417 172 11
Fragments arranged by size:
796 426 417 240 172 61 34 11
BsiWI C'GTAC_G
Cuts at: 0 35 2157
Size: 35 2122
Bsp1286I G_dGCh'C
Cuts at: 0 34 274 618 631 1070 1101 1119 1174
Size: 34 240 344 13 439 31 18 55
Cuts at: 1174 1393 1429 1496 1557 1715 1832 1848 1928
Size: 219 36 67 61 158 117 16 80
Cuts at: 1928 1974 2042 2087 2146 2157
Size: 46 68 45 59 11
Fragments arranged by size:
439 344 240 219 158 117 80 68
67 61 59 55 46 45 36 34
31 18 16 13 11
BspHI T'CATG_A
Cuts at: 0 770 2157
Size: 770 1387
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2008 2157
Size: 2008 149
BsrBI CCG'CTC
Cuts at: 0 1055 2157
Size: 1055 1102
BsrDI GCAATG_nn'
Cuts at: 0 869 2157
Size: 869 1288
BsrFI r'CCGG_y
Cuts at: 0 289 515 2157
Size: 289 226 1642
Fragments arranged by size:
1642 289 226
BsrGI T'GTAC_A
Cuts at: 0 1811 2157
Size: 1811 346
BssHII G'CGCG_C
Cuts at: 0 920 2157
Size: 920 1237
BssSI C'ACGA_G
Cuts at: 0 1569 2157
Size: 1569 588
BstAPI GCAn_nnn'nTGC
Cuts at: 0 331 1203 1836 1912 2157
Size: 331 872 633 76 245
Fragments arranged by size:
872 633 331 245 76
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1668 2157
Size: 1668 489
Bsu36I CC'TnA_GG
Cuts at: 0 928 2157
Size: 928 1229
BtgI C'CryG_G
Cuts at: 0 83 533 850 962 1356 1583 1854 2157
Size: 83 450 317 112 394 227 271 303
Fragments arranged by size:
450 394 317 303 271 227 112 83
BtsI GCAGTG_nn'
Cuts at: 0 1865 2157
Size: 1865 292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 410 445 2157
Size: 410 35 1712
Fragments arranged by size:
1712 410 35
DraIII CAC_nnn'GTG
Cuts at: 0 1836 1912 2157
Size: 1836 76 245
Fragments arranged by size:
1836 245 76
EaeI y'GGCC_r
Cuts at: 0 152 287 1089 2111 2157
Size: 152 135 802 1022 46
Fragments arranged by size:
1022 802 152 135 46
EagI C'GGCC_G
Cuts at: 0 287 2157
Size: 287 1870
EarI CTCTTCn'nnn_
Cuts at: 0 235 2157
Size: 235 1922
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1248 2157
Size: 1248 909
EcoICRI GAG'CTC
Cuts at: 0 272 1555 2157
Size: 272 1283 602
Fragments arranged by size:
1283 602 272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 1229 1241 1431 1571 1942 2157
Size: 361 868 12 190 140 371 215
Fragments arranged by size:
868 371 361 215 190 140 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 323 2157
Size: 323 1834
EcoO109I rG'GnC_Cy
Cuts at: 0 59 564 614 1115 1116 1214 1249 1626
Size: 59 505 50 501 1 98 35 377
Cuts at: 1626 2157
Size: 531
Fragments arranged by size:
531 505 501 377 98 59 50 35
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 264 2157
Size: 264 1893
HaeII r_GCGC'y
Cuts at: 0 296 829 2157
Size: 296 533 1328
Fragments arranged by size:
1328 533 296
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 169 201 691 723 1321 1353 1563 1595
Size: 169 32 490 32 598 32 210 32
Cuts at: 1595 2157
Size: 562
Fragments arranged by size:
598 562 490 210 169 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 199 2132 2157
Size: 199 1933 25
Fragments arranged by size:
1933 199 25
KasI G'GCGC_C
Cuts at: 0 292 825 2157
Size: 292 533 1332
Fragments arranged by size:
1332 533 292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 125 338 1663 1928 2157
Size: 122 3 213 1325 265 229
Fragments arranged by size:
1325 265 229 213 122 3
MscI TGG'CCA
Cuts at: 0 154 2113 2157
Size: 154 1959 44
Fragments arranged by size:
1959 154 44
MslI CAynn'nnrTG
Cuts at: 0 490 775 1125 1666 2055 2157
Size: 490 285 350 541 389 102
Fragments arranged by size:
541 490 389 350 285 102
MspA1I CmG'CkG
Cuts at: 0 314 668 744 852 901 964 1276 1358
Size: 314 354 76 108 49 63 312 82
Cuts at: 1358 2157
Size: 799
Fragments arranged by size:
799 354 314 312 108 82 76 63
49
NaeI GCC'GGC
Cuts at: 0 291 517 2157
Size: 291 226 1640
Fragments arranged by size:
1640 291 226
NarI GG'CG_CC
Cuts at: 0 293 826 2157
Size: 293 533 1331
Fragments arranged by size:
1331 533 293
NcoI C'CATG_G
Cuts at: 0 533 1583 1854 2157
Size: 533 1050 271 303
Fragments arranged by size:
1050 533 303 271
NgoMIV G'CCGG_C
Cuts at: 0 289 515 2157
Size: 289 226 1642
Fragments arranged by size:
1642 289 226
NsiI A_TGCA'T
Cuts at: 0 417 2157
Size: 417 1740
PflMI CCAn_nnn'nTGG
Cuts at: 0 2079 2157
Size: 2079 78
PfoI T'CCnGG_A
Cuts at: 0 1245 2157
Size: 1245 912
PmlI CAC'GTG
Cuts at: 0 492 2157
Size: 492 1665
PpuMI rG'GwC_Cy
Cuts at: 0 1249 1626 2157
Size: 1249 377 531
Fragments arranged by size:
1249 531 377
PshAI GACnn'nnGTC
Cuts at: 0 314 2157
Size: 314 1843
PspOMI G'GGCC_C
Cuts at: 0 614 1115 2157
Size: 614 501 1042
Fragments arranged by size:
1042 614 501
PstI C_TGCA'G
Cuts at: 0 68 1316 1532 1701 2005 2157
Size: 68 1248 216 169 304 152
Fragments arranged by size:
1248 304 216 169 152 68
PvuI CG_AT'CG
Cuts at: 0 428 2157
Size: 428 1729
PvuII CAG'CTG
Cuts at: 0 314 744 2157
Size: 314 430 1413
Fragments arranged by size:
1413 430 314
SacI G_AGCT'C
Cuts at: 0 274 1557 2157
Size: 274 1283 600
Fragments arranged by size:
1283 600 274
SacII CC_GC'GG
Cuts at: 0 853 965 1359 2157
Size: 853 112 394 798
Fragments arranged by size:
853 798 394 112
SapI GCTCTTCn'nnn_
Cuts at: 0 235 2157
Size: 235 1922
ScaI AGT'ACT
Cuts at: 0 649 2157
Size: 649 1508
SexAI A'CCwGG_T
Cuts at: 0 1487 2157
Size: 1487 670
SfcI C'TryA_G
Cuts at: 0 64 1312 1528 1697 2001 2157
Size: 64 1248 216 169 304 156
Fragments arranged by size:
1248 304 216 169 156 64
SfoI GGC'GCC
Cuts at: 0 294 827 2157
Size: 294 533 1330
Fragments arranged by size:
1330 533 294
SmaI CCC'GGG
Cuts at: 0 724 2088 2157
Size: 724 1364 69
Fragments arranged by size:
1364 724 69
StyI C'CwwG_G
Cuts at: 0 533 572 1163 1583 1854 2042 2108 2157
Size: 533 39 591 420 271 188 66 49
Fragments arranged by size:
591 533 420 271 188 66 49 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1673 1766 2157
Size: 1673 93 391
Fragments arranged by size:
1673 391 93
TatI w'GTAC_w
Cuts at: 0 647 1811 2157
Size: 647 1164 346
Fragments arranged by size:
1164 647 346
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 869 901 1669 1701 2157
Size: 869 32 768 32 456
Fragments arranged by size:
869 768 456 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1150 2157
Size: 1150 1007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 553 2106 2157
Size: 553 1553 51
Fragments arranged by size:
1553 553 51
XmaI C'CCGG_G
Cuts at: 0 722 2086 2157
Size: 722 1364 71
Fragments arranged by size:
1364 722 71
XmnI GAAnn'nnTTC
Cuts at: 0 244 2157
Size: 244 1913
Enzymes that do cut:
AarI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI
BanI BanII BbeI BbvCI BcgI BfrBI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstXI Bsu36I BtgI BtsI CspCI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HindIII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI PflMI PfoI PmlI PpuMI
PshAI PspOMI PstI PvuI PvuII SacI SacII SapI
ScaI SexAI SfcI SfoI SmaI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BciVI BclI BglII BmgBI BmtI BpuEI
BsaI BsaBI BsaWI BsmI BsmBI BspEI BstBI BstEII
BstYI BstZ17I BtgZI ClaI DraI DrdI EcoRI EcoRV
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NspI PacI PasI PciI
PmeI PpiI PsiI PspXI PsrI RsrII SalI SanDI
SbfI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XhoI ZraI