(Linear) (Six-base) MAPSORT of: KIAA1238.seq Check: 581 from: 1 to: 1158 KIAA1238.orf With 182 enzymes: SgfI * October 24, 2006 15:08 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 464 1158 Size: 464 694 AflIII A'CryG_T Cuts at: 0 612 1158 Size: 612 546 AhdI GACnn_n'nnGTC Cuts at: 0 41 1158 Size: 41 1117 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 808 1158 Size: 808 350 ApoI r'AATT_y Cuts at: 0 417 1158 Size: 417 741 AvaI C'yCGr_G Cuts at: 0 512 1158 Size: 512 646 BanI G'GyrC_C Cuts at: 0 643 1158 Size: 643 515 BbsI GAAGACnn'nnnn_ Cuts at: 0 62 1158 Size: 62 1096 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 403 437 1158 Size: 403 34 721 Fragments arranged by size: 721 403 34 BciVI GTATCCnnnnn_n' Cuts at: 0 384 1158 Size: 384 774 BmtI G_CTAG'C Cuts at: 0 745 1132 1158 Size: 745 387 26 Fragments arranged by size: 745 387 26 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1102 1158 Size: 1102 56 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 1158 Size: 472 686 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 302 1158 Size: 302 856 BsaAI yAC'GTr Cuts at: 0 613 1158 Size: 613 545 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 620 650 1158 Size: 620 30 508 Fragments arranged by size: 620 508 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 121 1158 Size: 121 1037 BseYI C'CCAG_C Cuts at: 0 996 1005 1158 Size: 996 9 153 Fragments arranged by size: 996 153 9 BsiHKAI G_wGCw'C Cuts at: 0 718 1158 Size: 718 440 BsmI GAATG_Cn' Cuts at: 0 680 1158 Size: 680 478 Bsp1286I G_dGCh'C Cuts at: 0 718 1158 Size: 718 440 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 800 1158 Size: 800 358 BsrFI r'CCGG_y Cuts at: 0 929 1158 Size: 929 229 BstAPI GCAn_nnn'nTGC Cuts at: 0 904 1158 Size: 904 254 BstXI CCAn_nnnn'nTGG Cuts at: 0 242 1158 Size: 242 916 BtgI C'CryG_G Cuts at: 0 372 959 1158 Size: 372 587 199 Fragments arranged by size: 587 372 199 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 837 1158 Size: 837 321 BtsI GCAGTG_nn' Cuts at: 0 200 968 1013 1158 Size: 200 768 45 145 Fragments arranged by size: 768 200 145 45 EaeI y'GGCC_r Cuts at: 0 369 408 927 1158 Size: 369 39 519 231 Fragments arranged by size: 519 369 231 39 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 464 472 1158 Size: 464 8 686 Fragments arranged by size: 686 464 8 EcoO109I rG'GnC_Cy Cuts at: 0 1000 1104 1158 Size: 1000 104 54 Fragments arranged by size: 1000 104 54 HaeII r_GCGC'y Cuts at: 0 565 1158 Size: 565 593 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 27 59 182 214 282 314 382 414 Size: 27 32 123 32 68 32 68 32 Cuts at: 414 796 828 1158 Size: 382 32 330 Fragments arranged by size: 382 330 123 68 68 32 32 32 32 32 27 HincII GTy'rAC Cuts at: 0 1023 1053 1158 Size: 1023 30 105 Fragments arranged by size: 1023 105 30 MscI TGG'CCA Cuts at: 0 371 410 1158 Size: 371 39 748 Fragments arranged by size: 748 371 39 MslI CAynn'nnrTG Cuts at: 0 622 1158 Size: 622 536 MspA1I CmG'CkG Cuts at: 0 1125 1158 Size: 1125 33 NaeI GCC'GGC Cuts at: 0 931 1158 Size: 931 227 NcoI C'CATG_G Cuts at: 0 372 1158 Size: 372 786 NgoMIV G'CCGG_C Cuts at: 0 929 1158 Size: 929 229 NheI G'CTAG_C Cuts at: 0 741 1128 1158 Size: 741 387 30 Fragments arranged by size: 741 387 30 PasI CC'CwG_GG Cuts at: 0 1099 1158 Size: 1099 59 PflMI CCAn_nnn'nTGG Cuts at: 0 26 1158 Size: 26 1132 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1023 1055 1158 Size: 1023 32 103 Fragments arranged by size: 1023 103 32 PpuMI rG'GwC_Cy Cuts at: 0 1000 1158 Size: 1000 158 PvuII CAG'CTG Cuts at: 0 1125 1158 Size: 1125 33 SanDI GG'GwC_CC Cuts at: 0 1000 1158 Size: 1000 158 SmlI C'TyrA_G Cuts at: 0 317 512 1158 Size: 317 195 646 Fragments arranged by size: 646 317 195 SpeI A'CTAG_T Cuts at: 0 982 1158 Size: 982 176 StyI C'CwwG_G Cuts at: 0 241 372 1158 Size: 241 131 786 Fragments arranged by size: 786 241 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 234 1158 Size: 234 924 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 620 652 1158 Size: 620 32 506 Fragments arranged by size: 620 506 32 XbaI T'CTAG_A Cuts at: 0 282 876 1158 Size: 282 594 282 Fragments arranged by size: 594 282 282 XhoI C'TCGA_G Cuts at: 0 512 1158 Size: 512 646 Enzymes that do cut: AcuI AflIII AhdI AlfI ApoI AvaI BanI BbsI BcgI BciVI BmtI BplI BpmI BpuEI BsaAI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrFI BstAPI BstXI BtgI BtgZI BtsI EaeI Eco57MI EcoO109I HaeII Hin4I HincII MscI MslI MspA1I NaeI NcoI NgoMIV NheI PasI PflMI PpiI PpuMI PvuII SanDI SmlI SpeI StyI TaqII TstI XbaI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI Bpu10I BsaI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI SwaI TatI Tth111I XcmI XmaI XmnI ZraI