Restriction map for pF1KA1238
 (Linear) (Six-base) MAPSORT of: KIAA1238.seq  Check: 581  from: 1  to: 1158



KIAA1238.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     464    1158 
   Size:        464     694
 
AflIII A'CryG_T

Cuts at:      0     612    1158 
   Size:        612     546
 
AhdI GACnn_n'nnGTC

Cuts at:      0      41    1158 
   Size:         41    1117
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     808    1158 
   Size:        808     350
 
ApoI r'AATT_y

Cuts at:      0     417    1158 
   Size:        417     741
 
AvaI C'yCGr_G

Cuts at:      0     512    1158 
   Size:        512     646
 
BanI G'GyrC_C

Cuts at:      0     643    1158 
   Size:        643     515
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      62    1158 
   Size:         62    1096
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     403     437    1158 
   Size:        403      34     721

  Fragments arranged by size: 

                 721     403      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     384    1158 
   Size:        384     774
 
BmtI G_CTAG'C

Cuts at:      0     745    1132    1158 
   Size:        745     387      26

  Fragments arranged by size: 

                 745     387      26
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1102    1158 
   Size:       1102      56
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472    1158 
   Size:        472     686
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302    1158 
   Size:        302     856
 
BsaAI yAC'GTr

Cuts at:      0     613    1158 
   Size:        613     545
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     620     650    1158 
   Size:        620      30     508

  Fragments arranged by size: 

                 620     508      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     121    1158 
   Size:        121    1037
 
BseYI C'CCAG_C

Cuts at:      0     996    1005    1158 
   Size:        996       9     153

  Fragments arranged by size: 

                 996     153       9
 
BsiHKAI G_wGCw'C

Cuts at:      0     718    1158 
   Size:        718     440
 
BsmI GAATG_Cn'

Cuts at:      0     680    1158 
   Size:        680     478
 
Bsp1286I G_dGCh'C

Cuts at:      0     718    1158 
   Size:        718     440
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     800    1158 
   Size:        800     358
 
BsrFI r'CCGG_y

Cuts at:      0     929    1158 
   Size:        929     229
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     904    1158 
   Size:        904     254
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     242    1158 
   Size:        242     916
 
BtgI C'CryG_G

Cuts at:      0     372     959    1158 
   Size:        372     587     199

  Fragments arranged by size: 

                 587     372     199
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     837    1158 
   Size:        837     321
 
BtsI GCAGTG_nn'

Cuts at:      0     200     968    1013    1158 
   Size:        200     768      45     145

  Fragments arranged by size: 

                 768     200     145      45
 
EaeI y'GGCC_r

Cuts at:      0     369     408     927    1158 
   Size:        369      39     519     231

  Fragments arranged by size: 

                 519     369     231      39
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     464     472    1158 
   Size:        464       8     686

  Fragments arranged by size: 

                 686     464       8
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1000    1104    1158 
   Size:       1000     104      54

  Fragments arranged by size: 

                1000     104      54
 
HaeII r_GCGC'y

Cuts at:      0     565    1158 
   Size:        565     593
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27      59     182     214     282     314     382     414 
   Size:         27      32     123      32      68      32      68      32

Cuts at:    414     796     828    1158 
   Size:        382      32     330

  Fragments arranged by size: 

                 382     330     123      68      68      32      32      32
                  32      32      27
 
HincII GTy'rAC

Cuts at:      0    1023    1053    1158 
   Size:       1023      30     105

  Fragments arranged by size: 

                1023     105      30
 
MscI TGG'CCA

Cuts at:      0     371     410    1158 
   Size:        371      39     748

  Fragments arranged by size: 

                 748     371      39
 
MslI CAynn'nnrTG

Cuts at:      0     622    1158 
   Size:        622     536
 
MspA1I CmG'CkG

Cuts at:      0    1125    1158 
   Size:       1125      33
 
NaeI GCC'GGC

Cuts at:      0     931    1158 
   Size:        931     227
 
NcoI C'CATG_G

Cuts at:      0     372    1158 
   Size:        372     786
 
NgoMIV G'CCGG_C

Cuts at:      0     929    1158 
   Size:        929     229
 
NheI G'CTAG_C

Cuts at:      0     741    1128    1158 
   Size:        741     387      30

  Fragments arranged by size: 

                 741     387      30
 
PasI CC'CwG_GG

Cuts at:      0    1099    1158 
   Size:       1099      59
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      26    1158 
   Size:         26    1132
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1023    1055    1158 
   Size:       1023      32     103

  Fragments arranged by size: 

                1023     103      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1000    1158 
   Size:       1000     158
 
PvuII CAG'CTG

Cuts at:      0    1125    1158 
   Size:       1125      33
 
SanDI GG'GwC_CC

Cuts at:      0    1000    1158 
   Size:       1000     158
 
SmlI C'TyrA_G

Cuts at:      0     317     512    1158 
   Size:        317     195     646

  Fragments arranged by size: 

                 646     317     195
 
SpeI A'CTAG_T

Cuts at:      0     982    1158 
   Size:        982     176
 
StyI C'CwwG_G

Cuts at:      0     241     372    1158 
   Size:        241     131     786

  Fragments arranged by size: 

                 786     241     131
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     234    1158 
   Size:        234     924
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     620     652    1158 
   Size:        620      32     506

  Fragments arranged by size: 

                 620     506      32
 
XbaI T'CTAG_A

Cuts at:      0     282     876    1158 
   Size:        282     594     282

  Fragments arranged by size: 

                 594     282     282
 
XhoI C'TCGA_G

Cuts at:      0     512    1158 
   Size:        512     646
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AlfI     ApoI     AvaI     BanI     BbsI
     BcgI    BciVI     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaXI
    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrFI   BstAPI
    BstXI     BtgI    BtgZI     BtsI     EaeI  Eco57MI EcoO109I    HaeII
    Hin4I   HincII     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
     NheI     PasI    PflMI     PpiI    PpuMI    PvuII    SanDI     SmlI
     SpeI     StyI    TaqII     TstI     XbaI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI   Bpu10I     BsaI
    BsaBI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NarI     NdeI     NotI     NruI     NsiI     NspI
     PacI     PciI     PfoI     PmeI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     StuI     SwaI
     TatI  Tth111I     XcmI     XmaI     XmnI     ZraI
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