Restriction map for
pF1KSDA1242
(Linear) (Six-base) MAPSORT of: KIAA1242.seq Check: 4099 from: 1 to: 1479
KIAA1242.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 628 1479
Size: 628 851
AfeI AGC'GCT
Cuts at: 0 406 1479
Size: 406 1073
AflIII A'CryG_T
Cuts at: 0 66 816 1479
Size: 66 750 663
Fragments arranged by size:
750 663 66
AhdI GACnn_n'nnGTC
Cuts at: 0 585 1479
Size: 585 894
AleI CACnn'nnGTG
Cuts at: 0 622 1179 1479
Size: 622 557 300
Fragments arranged by size:
622 557 300
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 895 1479
Size: 895 584
AlwNI CAG_nnn'CTG
Cuts at: 0 477 1479
Size: 477 1002
AvaI C'yCGr_G
Cuts at: 0 544 987 1026 1116 1479
Size: 544 443 39 90 363
Fragments arranged by size:
544 443 363 90 39
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1125 1158 1479
Size: 1125 33 321
Fragments arranged by size:
1125 321 33
BamHI G'GATC_C
Cuts at: 0 1472 1479
Size: 1472 7
BanI G'GyrC_C
Cuts at: 0 20 301 616 1479
Size: 20 281 315 863
Fragments arranged by size:
863 315 281 20
BanII G_rGCy'C
Cuts at: 0 956 1039 1198 1479
Size: 956 83 159 281
Fragments arranged by size:
956 281 159 83
BbeI G_GCGC'C
Cuts at: 0 24 1479
Size: 24 1455
BciVI GTATCCnnnnn_n'
Cuts at: 0 1153 1479
Size: 1153 326
BglI GCCn_nnn'nGGC
Cuts at: 0 1075 1479
Size: 1075 404
BglII A'GATC_T
Cuts at: 0 509 761 1479
Size: 509 252 718
Fragments arranged by size:
718 509 252
BlpI GC'TnA_GC
Cuts at: 0 601 842 1479
Size: 601 241 637
Fragments arranged by size:
637 601 241
Bme1580I G_kGCm'C
Cuts at: 0 742 1325 1479
Size: 742 583 154
Fragments arranged by size:
742 583 154
BmrI ACTGGGnnnn_n'
Cuts at: 0 103 206 1479
Size: 103 103 1273
Fragments arranged by size:
1273 103 103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 189 393 1406 1479
Size: 135 54 204 1013 73
Fragments arranged by size:
1013 204 135 73 54
Bpu10I CC'TnA_GC
Cuts at: 0 394 1033 1479
Size: 394 639 446
Fragments arranged by size:
639 446 394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 940 1479
Size: 268 672 539
Fragments arranged by size:
672 539 268
BsaI GGTCTCn'nnnn_
Cuts at: 0 128 1387 1479
Size: 128 1259 92
Fragments arranged by size:
1259 128 92
BsaAI yAC'GTr
Cuts at: 0 386 1479
Size: 386 1093
BsaBI GATnn'nnATC
Cuts at: 0 40 1479
Size: 40 1439
BsaHI Gr'CG_yC
Cuts at: 0 21 94 236 1479
Size: 21 73 142 1243
Fragments arranged by size:
1243 142 73 21
BsaWI w'CCGG_w
Cuts at: 0 221 482 1479
Size: 221 261 997
Fragments arranged by size:
997 261 221
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 627 657 1180 1210 1432 1462 1479
Size: 627 30 523 30 222 30 17
Fragments arranged by size:
627 523 222 30 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 970 1479
Size: 970 509
BseYI C'CCAG_C
Cuts at: 0 121 297 1479
Size: 121 176 1182
Fragments arranged by size:
1182 176 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 778 1063 1479
Size: 340 438 285 416
Fragments arranged by size:
438 416 340 285
BsiEI CG_ry'CG
Cuts at: 0 870 1070 1479
Size: 870 200 409
Fragments arranged by size:
870 409 200
BsiHKAI G_wGCw'C
Cuts at: 0 602 1061 1249 1457 1479
Size: 602 459 188 208 22
Fragments arranged by size:
602 459 208 188 22
BsmI GAATG_Cn'
Cuts at: 0 843 1479
Size: 843 636
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1382 1479
Size: 1382 97
Bsp1286I G_dGCh'C
Cuts at: 0 602 742 956 1039 1061 1198 1249 1325
Size: 602 140 214 83 22 137 51 76
Cuts at: 1325 1457 1479
Size: 132 22
Fragments arranged by size:
602 214 140 137 132 83 76 51
22 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 628 1479
Size: 628 851
BsrBI CCG'CTC
Cuts at: 0 18 717 918 1479
Size: 18 699 201 561
Fragments arranged by size:
699 561 201 18
BsrDI GCAATG_nn'
Cuts at: 0 1355 1479
Size: 1355 124
BsrFI r'CCGG_y
Cuts at: 0 304 935 1479
Size: 304 631 544
Fragments arranged by size:
631 544 304
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1107 1479
Size: 1107 372
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1239 1479
Size: 1239 240
BstYI r'GATC_y
Cuts at: 0 509 518 761 1271 1472 1479
Size: 509 9 243 510 201 7
Fragments arranged by size:
510 509 243 201 9 7
Bsu36I CC'TnA_GG
Cuts at: 0 1399 1479
Size: 1399 80
BtgI C'CryG_G
Cuts at: 0 701 1479
Size: 701 778
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1065 1479
Size: 1065 414
BtsI GCAGTG_nn'
Cuts at: 0 904 1174 1186 1479
Size: 904 270 12 293
Fragments arranged by size:
904 293 270 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 1440 1479
Size: 1440 39
EaeI y'GGCC_r
Cuts at: 0 1067 1307 1479
Size: 1067 240 172
Fragments arranged by size:
1067 240 172
EagI C'GGCC_G
Cuts at: 0 1067 1479
Size: 1067 412
EarI CTCTTCn'nnn_
Cuts at: 0 1468 1479
Size: 1468 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 99 1479
Size: 99 1380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 189 393 1406 1479
Size: 135 54 204 1013 73
Fragments arranged by size:
1013 204 135 73 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1300 1479
Size: 1300 179
EcoO109I rG'GnC_Cy
Cuts at: 0 324 1479
Size: 324 1155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 540 1479
Size: 540 939
HaeII r_GCGC'y
Cuts at: 0 24 408 829 1479
Size: 24 384 421 650
Fragments arranged by size:
650 421 384 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 226 258 571 603 835 867 1180 1212
Size: 226 32 313 32 232 32 313 32
Cuts at: 1212 1432 1464 1479
Size: 220 32 15
Fragments arranged by size:
313 313 232 226 220 32 32 32
32 32 15
KasI G'GCGC_C
Cuts at: 0 20 1479
Size: 20 1459
MluI A'CGCG_T
Cuts at: 0 66 816 1479
Size: 66 750 663
Fragments arranged by size:
750 663 66
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 893 1479
Size: 779 114 586
Fragments arranged by size:
779 586 114
MscI TGG'CCA
Cuts at: 0 1309 1479
Size: 1309 170
MslI CAynn'nnrTG
Cuts at: 0 76 622 1179 1479
Size: 76 546 557 300
Fragments arranged by size:
557 546 300 76
MspA1I CmG'CkG
Cuts at: 0 849 1132 1139 1479
Size: 849 283 7 340
Fragments arranged by size:
849 340 283 7
NaeI GCC'GGC
Cuts at: 0 306 1479
Size: 306 1173
NarI GG'CG_CC
Cuts at: 0 21 1479
Size: 21 1458
NgoMIV G'CCGG_C
Cuts at: 0 304 1479
Size: 304 1175
PflMI CCAn_nnn'nTGG
Cuts at: 0 1085 1479
Size: 1085 394
PfoI T'CCnGG_A
Cuts at: 0 856 1479
Size: 856 623
PpuMI rG'GwC_Cy
Cuts at: 0 324 1479
Size: 324 1155
PspXI vC'TCGA_Gb
Cuts at: 0 1026 1479
Size: 1026 453
PstI C_TGCA'G
Cuts at: 0 353 554 884 911 929 1181 1479
Size: 353 201 330 27 18 252 298
Fragments arranged by size:
353 330 298 252 201 27 18
PvuII CAG'CTG
Cuts at: 0 849 1132 1479
Size: 849 283 347
Fragments arranged by size:
849 347 283
SexAI A'CCwGG_T
Cuts at: 0 725 1479
Size: 725 754
SfcI C'TryA_G
Cuts at: 0 349 550 880 907 925 1177 1479
Size: 349 201 330 27 18 252 302
Fragments arranged by size:
349 330 302 252 201 27 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1075 1479
Size: 1075 404
SfoI GGC'GCC
Cuts at: 0 22 1479
Size: 22 1457
SmlI C'TyrA_G
Cuts at: 0 283 955 1026 1479
Size: 283 672 71 453
Fragments arranged by size:
672 453 283 71
StyI C'CwwG_G
Cuts at: 0 230 1479
Size: 230 1249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 289 1479
Size: 289 1190
TatI w'GTAC_w
Cuts at: 0 1049 1479
Size: 1049 430
XhoI C'TCGA_G
Cuts at: 0 1026 1479
Size: 1026 453
Enzymes that do cut:
AarI AfeI AflIII AhdI AleI AlfI AlwNI AvaI
BaeI BamHI BanI BanII BbeI BciVI BglI BglII
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I KasI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV PflMI PfoI PpuMI PspXI PstI
PvuII SexAI SfcI SfiI SfoI SmlI StyI TaqII
TatI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BbsI
BbvCI BcgI BclI BfrBI BmgBI BmtI BplI BsiWI
BspEI BspHI BsrGI BssHII BssSI BstBI BstEII BstZ17I
ClaI CspCI DraI DraIII EcoICRI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI