Restriction map for
pF1KA1248
(Linear) (Six-base) MAPSORT of: KIAA1248.seq Check: 5897 from: 1 to: 1071
KIAA1248.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AgeI A'CCGG_T
Cuts at: 0 983 1071
Size: 983 88
AlwNI CAG_nnn'CTG
Cuts at: 0 201 1071
Size: 201 870
ApaI G_GGCC'C
Cuts at: 0 1043 1071
Size: 1043 28
ApoI r'AATT_y
Cuts at: 0 363 585 1071
Size: 363 222 486
Fragments arranged by size:
486 363 222
AvaI C'yCGr_G
Cuts at: 0 1044 1071
Size: 1044 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 589 622 1071
Size: 589 33 449
Fragments arranged by size:
589 449 33
BanI G'GyrC_C
Cuts at: 0 124 1071
Size: 124 947
BanII G_rGCy'C
Cuts at: 0 285 578 1043 1071
Size: 285 293 465 28
Fragments arranged by size:
465 293 285 28
BglI GCCn_nnn'nGGC
Cuts at: 0 1047 1071
Size: 1047 24
Bme1580I G_kGCm'C
Cuts at: 0 1043 1052 1071
Size: 1043 9 19
Fragments arranged by size:
1043 19 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 440 1071
Size: 440 631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 1071
Size: 419 652
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1071
Size: 799 272
BsaI GGTCTCn'nnnn_
Cuts at: 0 674 737 944 1068 1071
Size: 674 63 207 124 3
Fragments arranged by size:
674 207 124 63 3
BsaHI Gr'CG_yC
Cuts at: 0 58 1071
Size: 58 1013
BsaWI w'CCGG_w
Cuts at: 0 537 829 983 1071
Size: 537 292 154 88
Fragments arranged by size:
537 292 154 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 272 302 374 404 1071
Size: 272 30 72 30 667
Fragments arranged by size:
667 272 72 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 1071
Size: 46 1025
BseYI C'CCAG_C
Cuts at: 0 144 842 1071
Size: 144 698 229
Fragments arranged by size:
698 229 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 983 1071
Size: 40 943 88
Fragments arranged by size:
943 88 40
BsiHKAI G_wGCw'C
Cuts at: 0 578 1071
Size: 578 493
Bsp1286I G_dGCh'C
Cuts at: 0 285 578 1043 1052 1071
Size: 285 293 465 9 19
Fragments arranged by size:
465 293 285 19 9
BspEI T'CCGG_A
Cuts at: 0 537 829 1071
Size: 537 292 242
Fragments arranged by size:
537 292 242
BspHI T'CATG_A
Cuts at: 0 756 1071
Size: 756 315
BsrBI CCG'CTC
Cuts at: 0 992 1071
Size: 992 79
BsrFI r'CCGG_y
Cuts at: 0 983 1071
Size: 983 88
BstAPI GCAn_nnn'nTGC
Cuts at: 0 18 1071
Size: 18 1053
Bsu36I CC'TnA_GG
Cuts at: 0 62 715 1071
Size: 62 653 356
Fragments arranged by size:
653 356 62
BtgI C'CryG_G
Cuts at: 0 1055 1071
Size: 1055 16
BtsI GCAGTG_nn'
Cuts at: 0 773 1071
Size: 773 298
DraIII CAC_nnn'GTG
Cuts at: 0 84 168 1071
Size: 84 84 903
Fragments arranged by size:
903 84 84
DrdI GACnn_nn'nnGTC
Cuts at: 0 930 1071
Size: 930 141
EaeI y'GGCC_r
Cuts at: 0 68 1071
Size: 68 1003
EarI CTCTTCn'nnn_
Cuts at: 0 269 534 566 1071
Size: 269 265 32 505
Fragments arranged by size:
505 269 265 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1071
Size: 19 1052
EcoICRI GAG'CTC
Cuts at: 0 576 1071
Size: 576 495
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 1071
Size: 419 652
EcoO109I rG'GnC_Cy
Cuts at: 0 1039 1040 1071
Size: 1039 1 31
Fragments arranged by size:
1039 31 1
EcoRV GAT'ATC
Cuts at: 0 304 708 1071
Size: 304 404 363
Fragments arranged by size:
404 363 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 392 424 692 724 1071
Size: 392 32 268 32 347
Fragments arranged by size:
392 347 268 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 469 1071
Size: 372 97 602
Fragments arranged by size:
602 372 97
MslI CAynn'nnrTG
Cuts at: 0 257 761 1071
Size: 257 504 310
Fragments arranged by size:
504 310 257
MspA1I CmG'CkG
Cuts at: 0 846 1071
Size: 846 225
NcoI C'CATG_G
Cuts at: 0 1055 1071
Size: 1055 16
NspI r_CATG'y
Cuts at: 0 222 628 1071
Size: 222 406 443
Fragments arranged by size:
443 406 222
PflMI CCAn_nnn'nTGG
Cuts at: 0 168 488 1071
Size: 168 320 583
Fragments arranged by size:
583 320 168
PspOMI G'GGCC_C
Cuts at: 0 1039 1071
Size: 1039 32
PstI C_TGCA'G
Cuts at: 0 14 356 1071
Size: 14 342 715
Fragments arranged by size:
715 342 14
PvuII CAG'CTG
Cuts at: 0 846 1071
Size: 846 225
SacI G_AGCT'C
Cuts at: 0 578 1071
Size: 578 493
SapI GCTCTTCn'nnn_
Cuts at: 0 566 1071
Size: 566 505
ScaI AGT'ACT
Cuts at: 0 883 1071
Size: 883 188
SfcI C'TryA_G
Cuts at: 0 10 352 1071
Size: 10 342 719
Fragments arranged by size:
719 342 10
SmaI CCC'GGG
Cuts at: 0 1046 1071
Size: 1046 25
SmlI C'TyrA_G
Cuts at: 0 814 1071
Size: 814 257
StuI AGG'CCT
Cuts at: 0 521 874 1071
Size: 521 353 197
Fragments arranged by size:
521 353 197
StyI C'CwwG_G
Cuts at: 0 482 549 1055 1071
Size: 482 67 506 16
Fragments arranged by size:
506 482 67 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 645 814 883 1071
Size: 645 169 69 188
Fragments arranged by size:
645 188 169 69
TatI w'GTAC_w
Cuts at: 0 605 653 881 1071
Size: 605 48 228 190
Fragments arranged by size:
605 228 190 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 272 304 1071
Size: 272 32 767
Fragments arranged by size:
767 272 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 489 1071
Size: 489 582
XmaI C'CCGG_G
Cuts at: 0 1044 1071
Size: 1044 27
XmnI GAAnn'nnTTC
Cuts at: 0 230 1029 1071
Size: 230 799 42
Fragments arranged by size:
799 230 42
Enzymes that do cut:
AgeI AlwNI ApaI ApoI AvaI BaeI BanI BanII
BglI Bme1580I BplI BpmI BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI
BsrBI BsrFI BstAPI Bsu36I BtgI BtsI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV Hin4I
MmeI MslI MspA1I NcoI NspI PflMI PspOMI PstI
PvuII SacI SapI ScaI SfcI SmaI SmlI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I
BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BspMI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
BtgZI ClaI CspCI DraI EagI EcoNI EcoRI FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI ZraI