Restriction map for
pF1KA1250
(Linear) (Six-base) MAPSORT of: KIAA1250.seq Check: 3799 from: 1 to: 5313
KIAA1250.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3325 5313
Size: 3325 1988
AccI GT'mk_AC
Cuts at: 0 4207 5313
Size: 4207 1106
AclI AA'CG_TT
Cuts at: 0 5214 5313
Size: 5214 99
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1946 2338 2558 2987 3784 3875 3966 4451
Size: 1946 392 220 429 797 91 91 485
Cuts at: 4451 5009 5313
Size: 558 304
Fragments arranged by size:
1946 797 558 485 429 392 304 220
91 91
AfeI AGC'GCT
Cuts at: 0 860 5313
Size: 860 4453
AflII C'TTAA_G
Cuts at: 0 4525 5313
Size: 4525 788
AflIII A'CryG_T
Cuts at: 0 1960 5313
Size: 1960 3353
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 494 5313
Size: 494 4819
AlwNI CAG_nnn'CTG
Cuts at: 0 762 2361 5313
Size: 762 1599 2952
Fragments arranged by size:
2952 1599 762
ApaI G_GGCC'C
Cuts at: 0 3329 3391 4201 5313
Size: 3329 62 810 1112
Fragments arranged by size:
3329 1112 810 62
ApaLI G'TGCA_C
Cuts at: 0 250 5313
Size: 250 5063
ApoI r'AATT_y
Cuts at: 0 662 2157 2560 3078 3621 3783 4112 5091
Size: 662 1495 403 518 543 162 329 979
Cuts at: 5091 5313
Size: 222
Fragments arranged by size:
1495 979 662 543 518 403 329 222
162
AseI AT'TA_AT
Cuts at: 0 169 5313
Size: 169 5144
AvaI C'yCGr_G
Cuts at: 0 3122 3579 4201 5313
Size: 3122 457 622 1112
Fragments arranged by size:
3122 1112 622 457
AvrII C'CTAG_G
Cuts at: 0 3265 5313
Size: 3265 2048
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3217 3250 5313
Size: 3217 33 2063
Fragments arranged by size:
3217 2063 33
BamHI G'GATC_C
Cuts at: 0 4419 5313
Size: 4419 894
BanI G'GyrC_C
Cuts at: 0 379 3270 3797 4021 5313
Size: 379 2891 527 224 1292
Fragments arranged by size:
2891 1292 527 379 224
BanII G_rGCy'C
Cuts at: 0 3329 3391 3953 4201 4276 5255 5313
Size: 3329 62 562 248 75 979 58
Fragments arranged by size:
3329 979 562 248 75 62 58
BbeI G_GCGC'C
Cuts at: 0 3274 5313
Size: 3274 2039
BbsI GAAGACnn'nnnn_
Cuts at: 0 1445 3089 3863 4949 5313
Size: 1445 1644 774 1086 364
Fragments arranged by size:
1644 1445 1086 774 364
BbvCI CC'TCA_GC
Cuts at: 0 1678 3392 3551 5313
Size: 1678 1714 159 1762
Fragments arranged by size:
1762 1714 1678 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1844 1878 5313
Size: 1844 34 3435
Fragments arranged by size:
3435 1844 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 801 2609 5313
Size: 801 1808 2704
Fragments arranged by size:
2704 1808 801
BglI GCCn_nnn'nGGC
Cuts at: 0 2263 4898 5313
Size: 2263 2635 415
Fragments arranged by size:
2635 2263 415
BglII A'GATC_T
Cuts at: 0 4225 5313
Size: 4225 1088
BlpI GC'TnA_GC
Cuts at: 0 4679 5313
Size: 4679 634
Bme1580I G_kGCm'C
Cuts at: 0 254 1338 3329 3391 4026 4201 5313
Size: 254 1084 1991 62 635 175 1112
Fragments arranged by size:
1991 1112 1084 635 254 175 62
BmgBI CAC'GTC
Cuts at: 0 3314 3481 5313
Size: 3314 167 1832
Fragments arranged by size:
3314 1832 167
BmrI ACTGGGnnnn_n'
Cuts at: 0 2892 3103 3601 4179 5313
Size: 2892 211 498 578 1134
Fragments arranged by size:
2892 1134 578 498 211
BmtI G_CTAG'C
Cuts at: 0 2900 5313
Size: 2900 2413
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 880 5313
Size: 880 4433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3939 4649 5054 5313
Size: 3939 710 405 259
Fragments arranged by size:
3939 710 405 259
Bpu10I CC'TnA_GC
Cuts at: 0 1678 3392 3551 5313
Size: 1678 1714 159 1762
Fragments arranged by size:
1762 1714 1678 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1411 5111 5313
Size: 1411 3700 202
Fragments arranged by size:
3700 1411 202
BsaAI yAC'GTr
Cuts at: 0 3846 5313
Size: 3846 1467
BsaBI GATnn'nnATC
Cuts at: 0 587 5313
Size: 587 4726
BsaHI Gr'CG_yC
Cuts at: 0 3271 3964 5313
Size: 3271 693 1349
Fragments arranged by size:
3271 1349 693
BsaWI w'CCGG_w
Cuts at: 0 2930 5247 5313
Size: 2930 2317 66
Fragments arranged by size:
2930 2317 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 569 599 5313
Size: 569 30 4714
Fragments arranged by size:
4714 569 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1708 3567 5313
Size: 1708 1859 1746
Fragments arranged by size:
1859 1746 1708
BseYI C'CCAG_C
Cuts at: 0 3594 3891 4820 5038 5313
Size: 3594 297 929 218 275
Fragments arranged by size:
3594 929 297 275 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 599 775 2182 4156 4780 5313
Size: 515 84 176 1407 1974 624 533
Fragments arranged by size:
1974 1407 624 533 515 176 84
BsiHKAI G_wGCw'C
Cuts at: 0 254 296 3307 3953 5070 5255 5281 5313
Size: 254 42 3011 646 1117 185 26 32
Fragments arranged by size:
3011 1117 646 254 185 42 32 26
BsmI GAATG_Cn'
Cuts at: 0 4969 5306 5313
Size: 4969 337 7
Fragments arranged by size:
4969 337 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2582 5313
Size: 2582 2731
Bsp1286I G_dGCh'C
Cuts at: 0 254 296 1338 3307 3329 3391 3953 4026
Size: 254 42 1042 1969 22 62 562 73
Cuts at: 4026 4201 4276 5070 5255 5281 5313
Size: 175 75 794 185 26 32
Fragments arranged by size:
1969 1042 794 562 254 185 175 75
73 62 42 32 26 22
BspEI T'CCGG_A
Cuts at: 0 5247 5313
Size: 5247 66
BspHI T'CATG_A
Cuts at: 0 2222 5313
Size: 2222 3091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3325 5313
Size: 3325 1988
BsrBI CCG'CTC
Cuts at: 0 3243 5313
Size: 3243 2070
BsrDI GCAATG_nn'
Cuts at: 0 2399 2556 4169 5313
Size: 2399 157 1613 1144
Fragments arranged by size:
2399 1613 1144 157
BsrGI T'GTAC_A
Cuts at: 0 404 5313
Size: 404 4909
BstAPI GCAn_nnn'nTGC
Cuts at: 0 762 5313
Size: 762 4551
BstBI TT'CG_AA
Cuts at: 0 3989 5313
Size: 3989 1324
BstEII G'GTnAC_C
Cuts at: 0 2775 5313
Size: 2775 2538
BstXI CCAn_nnnn'nTGG
Cuts at: 0 425 1383 5313
Size: 425 958 3930
Fragments arranged by size:
3930 958 425
BstYI r'GATC_y
Cuts at: 0 759 3162 4225 4419 4765 4908 5241 5313
Size: 759 2403 1063 194 346 143 333 72
Fragments arranged by size:
2403 1063 759 346 333 194 143 72
BtgI C'CryG_G
Cuts at: 0 572 5313
Size: 572 4741
BtsI GCAGTG_nn'
Cuts at: 0 635 1338 1586 2926 3891 4603 5313
Size: 635 703 248 1340 965 712 710
Fragments arranged by size:
1340 965 712 710 703 635 248
ClaI AT'CG_AT
Cuts at: 0 2318 4373 5313
Size: 2318 2055 940
Fragments arranged by size:
2318 2055 940
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1733 1768 2288 2323 5313
Size: 1733 35 520 35 2990
Fragments arranged by size:
2990 1733 520 35 35
EaeI y'GGCC_r
Cuts at: 0 111 569 2255 2487 2923 3729 5313
Size: 111 458 1686 232 436 806 1584
Fragments arranged by size:
1686 1584 806 458 436 232 111
EarI CTCTTCn'nnn_
Cuts at: 0 74 664 1240 1638 2030 2631 2954 3312
Size: 74 590 576 398 392 601 323 358
Cuts at: 3312 3986 4351 4510 5274 5313
Size: 674 365 159 764 39
Fragments arranged by size:
764 674 601 590 576 398 392 365
358 323 159 74 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4825 5313
Size: 4825 488
EcoICRI GAG'CTC
Cuts at: 0 3951 5253 5313
Size: 3951 1302 60
Fragments arranged by size:
3951 1302 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1946 2338 2558 2987 3784 3875 3939 3966
Size: 1946 392 220 429 797 91 64 27
Cuts at: 3966 4451 4649 5009 5054 5313
Size: 485 198 360 45 259
Fragments arranged by size:
1946 797 485 429 392 360 259 220
198 91 64 45 27
EcoO109I rG'GnC_Cy
Cuts at: 0 1674 3104 3259 3326 3388 4197 4198 5313
Size: 1674 1430 155 67 62 809 1 1115
Fragments arranged by size:
1674 1430 1115 809 155 67 62 1
EcoRI G'AATT_C
Cuts at: 0 2157 3621 5313
Size: 2157 1464 1692
Fragments arranged by size:
2157 1692 1464
EcoRV GAT'ATC
Cuts at: 0 14 960 5313
Size: 14 946 4353
Fragments arranged by size:
4353 946 14
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 88 5313
Size: 88 5225
FspI TGC'GCA
Cuts at: 0 2503 5313
Size: 2503 2810
HaeII r_GCGC'y
Cuts at: 0 827 862 3274 5313
Size: 827 35 2412 2039
Fragments arranged by size:
2412 2039 827 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1461 1493 2724 2756 2850 2882 3197 3229
Size: 1461 32 1231 32 94 32 315 32
Cuts at: 3229 3375 3407 4355 4366 4387 4398 4429 4461
Size: 146 32 948 11 21 11 31 32
Cuts at: 4461 4558 4590 4702 4734 5313
Size: 97 32 112 32 579
Fragments arranged by size:
1461 1231 948 579 315 146 112 97
94 32 32 32 32 32 32 32
32 31 21 11 11
HincII GTy'rAC
Cuts at: 0 285 489 2046 5313
Size: 285 204 1557 3267
Fragments arranged by size:
3267 1557 285 204
HindIII A'AGCT_T
Cuts at: 0 1772 4120 4465 4522 5313
Size: 1772 2348 345 57 791
Fragments arranged by size:
2348 1772 791 345 57
HpaI GTT'AAC
Cuts at: 0 285 5313
Size: 285 5028
KasI G'GCGC_C
Cuts at: 0 3270 5313
Size: 3270 2043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1639 3052 5313
Size: 1639 1413 2261
Fragments arranged by size:
2261 1639 1413
MscI TGG'CCA
Cuts at: 0 113 571 2257 2489 2925 5313
Size: 113 458 1686 232 436 2388
Fragments arranged by size:
2388 1686 458 436 232 113
MslI CAynn'nnrTG
Cuts at: 0 1045 1381 3300 5313
Size: 1045 336 1919 2013
Fragments arranged by size:
2013 1919 1045 336
MspA1I CmG'CkG
Cuts at: 0 3377 4868 5313
Size: 3377 1491 445
Fragments arranged by size:
3377 1491 445
NarI GG'CG_CC
Cuts at: 0 3271 5313
Size: 3271 2042
NcoI C'CATG_G
Cuts at: 0 572 5313
Size: 572 4741
NdeI CA'TA_TG
Cuts at: 0 780 5313
Size: 780 4533
NheI G'CTAG_C
Cuts at: 0 2896 5313
Size: 2896 2417
NspI r_CATG'y
Cuts at: 0 250 335 1311 1964 2502 5009 5313
Size: 250 85 976 653 538 2507 304
Fragments arranged by size:
2507 976 653 538 304 250 85
PacI TTA_AT'TAA
Cuts at: 0 173 5313
Size: 173 5140
PciI A'CATG_T
Cuts at: 0 1960 5313
Size: 1960 3353
PflMI CCAn_nnn'nTGG
Cuts at: 0 1570 5313
Size: 1570 3743
PmlI CAC'GTG
Cuts at: 0 3846 5313
Size: 3846 1467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2773 2805 5130 5162 5313
Size: 2773 32 2325 32 151
Fragments arranged by size:
2773 2325 151 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1674 3104 3259 5313
Size: 1674 1430 155 2054
Fragments arranged by size:
2054 1674 1430 155
PshAI GACnn'nnGTC
Cuts at: 0 1281 2801 5313
Size: 1281 1520 2512
Fragments arranged by size:
2512 1520 1281
PsiI TTA'TAA
Cuts at: 0 1018 1223 5313
Size: 1018 205 4090
Fragments arranged by size:
4090 1018 205
PspOMI G'GGCC_C
Cuts at: 0 3325 3387 4197 5313
Size: 3325 62 810 1116
Fragments arranged by size:
3325 1116 810 62
PspXI vC'TCGA_Gb
Cuts at: 0 3579 5313
Size: 3579 1734
SacI G_AGCT'C
Cuts at: 0 3953 5255 5313
Size: 3953 1302 58
Fragments arranged by size:
3953 1302 58
SapI GCTCTTCn'nnn_
Cuts at: 0 74 3312 3986 5313
Size: 74 3238 674 1327
Fragments arranged by size:
3238 1327 674 74
ScaI AGT'ACT
Cuts at: 0 3818 5313
Size: 3818 1495
SfcI C'TryA_G
Cuts at: 0 1088 1233 2093 2609 4158 5313
Size: 1088 145 860 516 1549 1155
Fragments arranged by size:
1549 1155 1088 860 516 145
SfoI GGC'GCC
Cuts at: 0 3272 5313
Size: 3272 2041
SmaI CCC'GGG
Cuts at: 0 4203 5313
Size: 4203 1110
SmlI C'TyrA_G
Cuts at: 0 1426 3122 3579 4525 5126 5313
Size: 1426 1696 457 946 601 187
Fragments arranged by size:
1696 1426 946 601 457 187
SphI G_CATG'C
Cuts at: 0 5009 5313
Size: 5009 304
SspI AAT'ATT
Cuts at: 0 1227 1265 2832 3024 5313
Size: 1227 38 1567 192 2289
Fragments arranged by size:
2289 1567 1227 192 38
StuI AGG'CCT
Cuts at: 0 5142 5313
Size: 5142 171
StyI C'CwwG_G
Cuts at: 0 572 2655 3265 3442 5313
Size: 572 2083 610 177 1871
Fragments arranged by size:
2083 1871 610 572 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1389 1577 3582 5313
Size: 1389 188 2005 1731
Fragments arranged by size:
2005 1731 1389 188
TatI w'GTAC_w
Cuts at: 0 404 3816 5313
Size: 404 3412 1497
Fragments arranged by size:
3412 1497 404
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 569 601 767 799 1565 1597 4299 4331
Size: 569 32 166 32 766 32 2702 32
Cuts at: 4331 5313
Size: 982
Fragments arranged by size:
2702 982 766 569 166 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 2347 2368 5313
Size: 2347 21 2945
Fragments arranged by size:
2945 2347 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1567 5313
Size: 1567 3746
XhoI C'TCGA_G
Cuts at: 0 3122 3579 5313
Size: 3122 457 1734
Fragments arranged by size:
3122 1734 457
XmaI C'CCGG_G
Cuts at: 0 4201 5313
Size: 4201 1112
XmnI GAAnn'nnTTC
Cuts at: 0 50 851 1417 1453 2640 2972 5306 5313
Size: 50 801 566 36 1187 332 2334 7
Fragments arranged by size:
2334 1187 801 566 332 50 36 7
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflII AflIII AlfI
AlwNI ApaI ApaLI ApoI AseI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstEII
BstXI BstYI BtgI BtsI ClaI CspCI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NspI PacI
PciI PflMI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI SacI SapI ScaI SfcI SfoI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AgeI AhdI AleI AloI AscI AsiSI
BclI BfrBI BsaI BsiEI BsiWI BsrFI BssHII BssSI
BstZ17I Bsu36I BtgZI DraI DraIII DrdI EagI EcoNI
FseI FspAI KpnI MfeI MluI NaeI NgoMIV NotI
NruI NsiI PasI PfoI PmeI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI SexAI SfiI
SgfI SgrAI SnaBI SpeI SrfI SwaI XbaI ZraI