Restriction map for
pF1KA1254
(Linear) (Six-base) MAPSORT of: KIAA1254.seq Check: 9154 from: 1 to: 2271
KIAA1254.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AccI GT'mk_AC
Cuts at: 0 430 2271
Size: 430 1841
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 590 887 2271
Size: 545 45 297 1384
Fragments arranged by size:
1384 545 297 45
AflII C'TTAA_G
Cuts at: 0 1060 2271
Size: 1060 1211
AflIII A'CryG_T
Cuts at: 0 1988 2271
Size: 1988 283
AleI CACnn'nnGTG
Cuts at: 0 1939 2271
Size: 1939 332
AlwNI CAG_nnn'CTG
Cuts at: 0 451 568 2271
Size: 451 117 1703
Fragments arranged by size:
1703 451 117
ApoI r'AATT_y
Cuts at: 0 9 81 1491 1570 1804 1821 1860 1963
Size: 9 72 1410 79 234 17 39 103
Cuts at: 1963 2271
Size: 308
Fragments arranged by size:
1410 308 234 103 79 72 39 17
9
BanI G'GyrC_C
Cuts at: 0 1725 2271
Size: 1725 546
BanII G_rGCy'C
Cuts at: 0 113 412 2271
Size: 113 299 1859
Fragments arranged by size:
1859 299 113
BbsI GAAGACnn'nnnn_
Cuts at: 0 281 401 553 578 2271
Size: 281 120 152 25 1693
Fragments arranged by size:
1693 281 152 120 25
BciVI GTATCCnnnnn_n'
Cuts at: 0 945 1998 2271
Size: 945 1053 273
Fragments arranged by size:
1053 945 273
BclI T'GATC_A
Cuts at: 0 2031 2076 2271
Size: 2031 45 195
Fragments arranged by size:
2031 195 45
BglI GCCn_nnn'nGGC
Cuts at: 0 188 2271
Size: 188 2083
BmrI ACTGGGnnnn_n'
Cuts at: 0 506 783 2271
Size: 506 277 1488
Fragments arranged by size:
1488 506 277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1177 1183 2271
Size: 961 216 6 1088
Fragments arranged by size:
1088 961 216 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 2271
Size: 346 1925
BsaI GGTCTCn'nnnn_
Cuts at: 0 646 2271
Size: 646 1625
BsaBI GATnn'nnATC
Cuts at: 0 676 2271
Size: 676 1595
BsaHI Gr'CG_yC
Cuts at: 0 531 2271
Size: 531 1740
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1108 1138 2271
Size: 1108 30 1133
Fragments arranged by size:
1133 1108 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 2271
Size: 157 2114
BsiHKAI G_wGCw'C
Cuts at: 0 1908 2271
Size: 1908 363
Bsp1286I G_dGCh'C
Cuts at: 0 113 412 1908 2271
Size: 113 299 1496 363
Fragments arranged by size:
1496 363 299 113
BspHI T'CATG_A
Cuts at: 0 54 2271
Size: 54 2217
BsrDI GCAATG_nn'
Cuts at: 0 154 2271
Size: 154 2117
BsrFI r'CCGG_y
Cuts at: 0 575 2271
Size: 575 1696
BstBI TT'CG_AA
Cuts at: 0 1319 2271
Size: 1319 952
BstXI CCAn_nnnn'nTGG
Cuts at: 0 703 2271
Size: 703 1568
BstZ17I GTA'TAC
Cuts at: 0 431 2271
Size: 431 1840
DraI TTT'AAA
Cuts at: 0 1608 1800 2271
Size: 1608 192 471
Fragments arranged by size:
1608 471 192
EaeI y'GGCC_r
Cuts at: 0 693 2271
Size: 693 1578
EarI CTCTTCn'nnn_
Cuts at: 0 417 971 1070 2092 2271
Size: 417 554 99 1022 179
Fragments arranged by size:
1022 554 417 179 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 590 887 961 1177 1183 2271
Size: 545 45 297 74 216 6 1088
Fragments arranged by size:
1088 545 297 216 74 45 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 943 2271
Size: 943 1328
EcoO109I rG'GnC_Cy
Cuts at: 0 1753 2271
Size: 1753 518
EcoRI G'AATT_C
Cuts at: 0 81 1491 1804 2271
Size: 81 1410 313 467
Fragments arranged by size:
1410 467 313 81
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 163 859 900 1332 1644 2137 2271
Size: 163 696 41 432 312 493 134
Fragments arranged by size:
696 493 432 312 163 134 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 466 498 609 641 811 843 865 897
Size: 466 32 111 32 170 32 22 32
Cuts at: 897 2271
Size: 1374
Fragments arranged by size:
1374 466 170 111 32 32 32 32
22
HincII GTy'rAC
Cuts at: 0 730 796 1127 2271
Size: 730 66 331 1144
Fragments arranged by size:
1144 730 331 66
MfeI C'AATT_G
Cuts at: 0 245 2271
Size: 245 2026
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 835 2153 2271
Size: 19 816 1318 118
Fragments arranged by size:
1318 816 118 19
MscI TGG'CCA
Cuts at: 0 695 2271
Size: 695 1576
MslI CAynn'nnrTG
Cuts at: 0 701 755 1939 2271
Size: 701 54 1184 332
Fragments arranged by size:
1184 701 332 54
MspA1I CmG'CkG
Cuts at: 0 104 1675 2271
Size: 104 1571 596
Fragments arranged by size:
1571 596 104
NdeI CA'TA_TG
Cuts at: 0 2223 2271
Size: 2223 48
NspI r_CATG'y
Cuts at: 0 1992 2271
Size: 1992 279
PciI A'CATG_T
Cuts at: 0 1988 2271
Size: 1988 283
PshAI GACnn'nnGTC
Cuts at: 0 2232 2271
Size: 2232 39
PsiI TTA'TAA
Cuts at: 0 824 2271
Size: 824 1447
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 251 283 2271
Size: 251 32 1988
Fragments arranged by size:
1988 251 32
PvuII CAG'CTG
Cuts at: 0 104 1675 2271
Size: 104 1571 596
Fragments arranged by size:
1571 596 104
SapI GCTCTTCn'nnn_
Cuts at: 0 417 2092 2271
Size: 417 1675 179
Fragments arranged by size:
1675 417 179
ScaI AGT'ACT
Cuts at: 0 1032 2271
Size: 1032 1239
SfcI C'TryA_G
Cuts at: 0 911 1730 2271
Size: 911 819 541
Fragments arranged by size:
911 819 541
SmlI C'TyrA_G
Cuts at: 0 325 1060 2271
Size: 325 735 1211
Fragments arranged by size:
1211 735 325
SpeI A'CTAG_T
Cuts at: 0 1170 2271
Size: 1170 1101
SspI AAT'ATT
Cuts at: 0 1010 2271
Size: 1010 1261
StyI C'CwwG_G
Cuts at: 0 1499 2271
Size: 1499 772
TatI w'GTAC_w
Cuts at: 0 1030 2271
Size: 1030 1241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2197 2229 2271
Size: 2197 32 42
Fragments arranged by size:
2197 42 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1207 2271
Size: 1207 1064
XmnI GAAnn'nnTTC
Cuts at: 0 1570 2086 2271
Size: 1570 516 185
Fragments arranged by size:
1570 516 185
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlwNI ApoI BanI
BanII BbsI BciVI BclI BglI BmrI BpmI BpuEI
BsaI BsaBI BsaHI BsaXI BseRI BsiHKAI Bsp1286I BspHI
BsrDI BsrFI BstBI BstXI BstZ17I DraI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII MfeI
MmeI MscI MslI MspA1I NdeI NspI PciI PshAI
PsiI PsrI PvuII SapI ScaI SfcI SmlI SpeI
SspI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BfrBI BglII BlpI
Bme1580I BmgBI BmtI BplI Bpu10I BsaAI BsaWI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrGI BssHII BssSI BstAPI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI
EcoICRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI StuI SwaI
TaqII Tth111I XbaI XhoI XmaI ZraI