Restriction map for
pF1KA1255
(Linear) (Six-base) MAPSORT of: KIAA1255.seq Check: 3522 from: 1 to: 3633
KIAA1255.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2712 3633
Size: 2712 921
AatII G_ACGT'C
Cuts at: 0 3519 3633
Size: 3519 114
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 1854 2284 3633
Size: 355 1499 430 1349
Fragments arranged by size:
1499 1349 430 355
AfeI AGC'GCT
Cuts at: 0 223 744 3633
Size: 223 521 2889
Fragments arranged by size:
2889 521 223
AflIII A'CryG_T
Cuts at: 0 1239 2165 3633
Size: 1239 926 1468
Fragments arranged by size:
1468 1239 926
AhdI GACnn_n'nnGTC
Cuts at: 0 1980 3633
Size: 1980 1653
AleI CACnn'nnGTG
Cuts at: 0 362 3633
Size: 362 3271
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 380 1211 3280 3283 3633
Size: 380 831 2069 3 350
Fragments arranged by size:
2069 831 380 350 3
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 508 540 3633
Size: 508 32 3093
Fragments arranged by size:
3093 508 32
AlwNI CAG_nnn'CTG
Cuts at: 0 623 1284 1950 2369 2976 3396 3633
Size: 623 661 666 419 607 420 237
Fragments arranged by size:
666 661 623 607 420 419 237
ApaI G_GGCC'C
Cuts at: 0 81 3633
Size: 81 3552
ApaLI G'TGCA_C
Cuts at: 0 3108 3462 3633
Size: 3108 354 171
Fragments arranged by size:
3108 354 171
ApoI r'AATT_y
Cuts at: 0 2742 2817 3633
Size: 2742 75 816
Fragments arranged by size:
2742 816 75
AvaI C'yCGr_G
Cuts at: 0 81 1886 2153 3334 3633
Size: 81 1805 267 1181 299
Fragments arranged by size:
1805 1181 299 267 81
BanI G'GyrC_C
Cuts at: 0 116 1087 1195 1567 1666 2294 2398 2503
Size: 116 971 108 372 99 628 104 105
Cuts at: 2503 3633
Size: 1130
Fragments arranged by size:
1130 971 628 372 116 108 105 104
99
BanII G_rGCy'C
Cuts at: 0 81 1655 1957 2342 3330 3633
Size: 81 1574 302 385 988 303
Fragments arranged by size:
1574 988 385 303 302 81
BbeI G_GCGC'C
Cuts at: 0 1670 2402 3633
Size: 1670 732 1231
Fragments arranged by size:
1670 1231 732
BbsI GAAGACnn'nnnn_
Cuts at: 0 413 819 824 1361 2482 3511 3633
Size: 413 406 5 537 1121 1029 122
Fragments arranged by size:
1121 1029 537 413 406 122 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 456 490 3633
Size: 456 34 3143
Fragments arranged by size:
3143 456 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3239 3633
Size: 3239 394
BclI T'GATC_A
Cuts at: 0 764 2795 3633
Size: 764 2031 838
Fragments arranged by size:
2031 838 764
BfrBI ATG'CAT
Cuts at: 0 3218 3633
Size: 3218 415
BglI GCCn_nnn'nGGC
Cuts at: 0 41 85 298 3633
Size: 41 44 213 3335
Fragments arranged by size:
3335 213 44 41
BglII A'GATC_T
Cuts at: 0 894 906 1026 3633
Size: 894 12 120 2607
Fragments arranged by size:
2607 894 120 12
BlpI GC'TnA_GC
Cuts at: 0 2717 3633
Size: 2717 916
Bme1580I G_kGCm'C
Cuts at: 0 81 1092 1572 3112 3466 3633
Size: 81 1011 480 1540 354 167
Fragments arranged by size:
1540 1011 480 354 167 81
BmgBI CAC'GTC
Cuts at: 0 822 2376 3633
Size: 822 1554 1257
Fragments arranged by size:
1554 1257 822
BmrI ACTGGGnnnn_n'
Cuts at: 0 1093 3633
Size: 1093 2540
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1045 3633
Size: 1045 2588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 523 949 1014 1544 1834 1979 2071
Size: 423 100 426 65 530 290 145 92
Cuts at: 2071 2086 2245 2515 3633
Size: 15 159 270 1118
Fragments arranged by size:
1118 530 426 423 290 270 159 145
100 92 65 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 1883 3633
Size: 1883 1750
BsaAI yAC'GTr
Cuts at: 0 2550 3633
Size: 2550 1083
BsaBI GATnn'nnATC
Cuts at: 0 452 3633
Size: 452 3181
BsaHI Gr'CG_yC
Cuts at: 0 125 1667 1759 2399 3119 3516 3633
Size: 125 1542 92 640 720 397 117
Fragments arranged by size:
1542 720 640 397 125 117 92
BsaWI w'CCGG_w
Cuts at: 0 1791 1863 3317 3633
Size: 1791 72 1454 316
Fragments arranged by size:
1791 1454 316 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2903 2933 3418 3448 3633
Size: 2903 30 485 30 185
Fragments arranged by size:
2903 485 185 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 562 664 1037 2171 3633
Size: 562 102 373 1134 1462
Fragments arranged by size:
1462 1134 562 373 102
BseYI C'CCAG_C
Cuts at: 0 854 1902 1943 1950 2273 2966 3633
Size: 854 1048 41 7 323 693 667
Fragments arranged by size:
1048 854 693 667 323 41 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 704 1090 1372 1750 1987 2296 2839
Size: 87 617 386 282 378 237 309 543
Cuts at: 2839 3139 3633
Size: 300 494
Fragments arranged by size:
617 543 494 386 378 309 300 282
237 87
BsiEI CG_ry'CG
Cuts at: 0 24 1937 3075 3322 3633
Size: 24 1913 1138 247 311
Fragments arranged by size:
1913 1138 311 247 24
BsiHKAI G_wGCw'C
Cuts at: 0 369 1655 2057 3112 3466 3633
Size: 369 1286 402 1055 354 167
Fragments arranged by size:
1286 1055 402 369 354 167
BsmI GAATG_Cn'
Cuts at: 0 663 3189 3220 3633
Size: 663 2526 31 413
Fragments arranged by size:
2526 663 413 31
Bsp1286I G_dGCh'C
Cuts at: 0 81 369 1092 1572 1655 1957 2057 2342
Size: 81 288 723 480 83 302 100 285
Cuts at: 2342 3112 3330 3466 3633
Size: 770 218 136 167
Fragments arranged by size:
770 723 480 302 288 285 218 167
136 100 83 81
BspEI T'CCGG_A
Cuts at: 0 1791 1863 3633
Size: 1791 72 1770
Fragments arranged by size:
1791 1770 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 35 1688 2712 2984 3633
Size: 35 1653 1024 272 649
Fragments arranged by size:
1653 1024 649 272 35
BsrFI r'CCGG_y
Cuts at: 0 23 55 110 3074 3633
Size: 23 32 55 2964 559
Fragments arranged by size:
2964 559 55 32 23
BsrGI T'GTAC_A
Cuts at: 0 1121 3633
Size: 1121 2512
BssSI C'ACGA_G
Cuts at: 0 1321 3633
Size: 1321 2312
BstAPI GCAn_nnn'nTGC
Cuts at: 0 165 2524 2976 3633
Size: 165 2359 452 657
Fragments arranged by size:
2359 657 452 165
BstBI TT'CG_AA
Cuts at: 0 1394 3633
Size: 1394 2239
BstXI CCAn_nnnn'nTGG
Cuts at: 0 807 862 948 2550 3633
Size: 807 55 86 1602 1083
Fragments arranged by size:
1602 1083 807 86 55
BstYI r'GATC_y
Cuts at: 0 485 894 906 1026 3633
Size: 485 409 12 120 2607
Fragments arranged by size:
2607 485 409 120 12
Bsu36I CC'TnA_GG
Cuts at: 0 458 574 715 3633
Size: 458 116 141 2918
Fragments arranged by size:
2918 458 141 116
BtgI C'CryG_G
Cuts at: 0 10 72 1406 3026 3433 3633
Size: 10 62 1334 1620 407 200
Fragments arranged by size:
1620 1334 407 200 62 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2217 2923 3633
Size: 2217 706 710
Fragments arranged by size:
2217 710 706
BtsI GCAGTG_nn'
Cuts at: 0 399 1102 1294 1355 1762 2370 3151 3464
Size: 399 703 192 61 407 608 781 313
Cuts at: 3464 3507 3633
Size: 43 126
Fragments arranged by size:
781 703 608 407 399 313 192 126
61 43
DraIII CAC_nnn'GTG
Cuts at: 0 1087 1113 1619 3633
Size: 1087 26 506 2014
Fragments arranged by size:
2014 1087 506 26
EaeI y'GGCC_r
Cuts at: 0 21 143 290 1253 1634 2009 2108 2552
Size: 21 122 147 963 381 375 99 444
Cuts at: 2552 3072 3191 3633
Size: 520 119 442
Fragments arranged by size:
963 520 444 442 381 375 147 122
119 99 21
EagI C'GGCC_G
Cuts at: 0 21 3072 3633
Size: 21 3051 561
Fragments arranged by size:
3051 561 21
EarI CTCTTCn'nnn_
Cuts at: 0 1838 2047 2414 2468 3056 3211 3633
Size: 1838 209 367 54 588 155 422
Fragments arranged by size:
1838 588 422 367 209 155 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 223 1753 3633
Size: 223 1530 1880
Fragments arranged by size:
1880 1530 223
EcoICRI GAG'CTC
Cuts at: 0 1653 3633
Size: 1653 1980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 423 523 949 1014 1544 1834 1854
Size: 355 68 100 426 65 530 290 20
Cuts at: 1854 1979 2071 2086 2245 2284 2515 3633
Size: 125 92 15 159 39 231 1118
Fragments arranged by size:
1118 530 426 355 290 231 159 125
100 92 68 65 39 20 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 718 1186 3633
Size: 718 468 2447
Fragments arranged by size:
2447 718 468
EcoO109I rG'GnC_Cy
Cuts at: 0 47 77 78 1061 1179 2284 2973 3633
Size: 47 30 1 983 118 1105 689 660
Fragments arranged by size:
1105 983 689 660 118 47 30 1
EcoRI G'AATT_C
Cuts at: 0 2817 3633
Size: 2817 816
EcoRV GAT'ATC
Cuts at: 0 2607 3633
Size: 2607 1026
FspI TGC'GCA
Cuts at: 0 242 3633
Size: 242 3391
HaeII r_GCGC'y
Cuts at: 0 225 746 1670 2402 3633
Size: 225 521 924 732 1231
Fragments arranged by size:
1231 924 732 521 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1138 1170 1705 1737 1786 1818 1952 1966
Size: 1138 32 535 32 49 32 134 14
Cuts at: 1966 1984 1998 2221 2253 3347 3379 3403 3435
Size: 18 14 223 32 1094 32 24 32
Cuts at: 3435 3633
Size: 198
Fragments arranged by size:
1138 1094 535 223 198 134 49 32
32 32 32 32 32 24 18 14
14
HincII GTy'rAC
Cuts at: 0 1071 1578 2846 3360 3633
Size: 1071 507 1268 514 273
Fragments arranged by size:
1268 1071 514 507 273
KasI G'GCGC_C
Cuts at: 0 1666 2398 3633
Size: 1666 732 1235
Fragments arranged by size:
1666 1235 732
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 416 1964 3426 3633
Size: 131 285 1548 1462 207
Fragments arranged by size:
1548 1462 285 207 131
MscI TGG'CCA
Cuts at: 0 145 292 1636 2011 2110 2554 3633
Size: 145 147 1344 375 99 444 1079
Fragments arranged by size:
1344 1079 444 375 147 145 99
MslI CAynn'nnrTG
Cuts at: 0 362 670 805 1793 2170 2258 2548 2615
Size: 362 308 135 988 377 88 290 67
Cuts at: 2615 2810 3633
Size: 195 823
Fragments arranged by size:
988 823 377 362 308 290 195 135
88 67
MspA1I CmG'CkG
Cuts at: 0 12 74 401 1281 1515 1522 1947 2019
Size: 12 62 327 880 234 7 425 72
Cuts at: 2019 2106 2199 2586 3190 3633
Size: 87 93 387 604 443
Fragments arranged by size:
880 604 443 425 387 327 234 93
87 72 62 12 7
NaeI GCC'GGC
Cuts at: 0 25 112 3076 3633
Size: 25 87 2964 557
Fragments arranged by size:
2964 557 87 25
NarI GG'CG_CC
Cuts at: 0 1667 2399 3633
Size: 1667 732 1234
Fragments arranged by size:
1667 1234 732
NdeI CA'TA_TG
Cuts at: 0 2146 3633
Size: 2146 1487
NgoMIV G'CCGG_C
Cuts at: 0 23 110 3074 3633
Size: 23 87 2964 559
Fragments arranged by size:
2964 559 87 23
NsiI A_TGCA'T
Cuts at: 0 3220 3633
Size: 3220 413
NspI r_CATG'y
Cuts at: 0 1212 1243 1929 2169 2273 3222 3633
Size: 1212 31 686 240 104 949 411
Fragments arranged by size:
1212 949 686 411 240 104 31
PasI CC'CwG_GG
Cuts at: 0 862 3633
Size: 862 2771
PciI A'CATG_T
Cuts at: 0 1239 2165 3633
Size: 1239 926 1468
Fragments arranged by size:
1468 1239 926
PflMI CCAn_nnn'nTGG
Cuts at: 0 1950 2273 3633
Size: 1950 323 1360
Fragments arranged by size:
1950 1360 323
PfoI T'CCnGG_A
Cuts at: 0 1811 2280 2828 3633
Size: 1811 469 548 805
Fragments arranged by size:
1811 805 548 469
PmlI CAC'GTG
Cuts at: 0 2550 3633
Size: 2550 1083
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 3633
Size: 490 32 3111
Fragments arranged by size:
3111 490 32
PpuMI rG'GwC_Cy
Cuts at: 0 47 2284 2973 3633
Size: 47 2237 689 660
Fragments arranged by size:
2237 689 660 47
PsiI TTA'TAA
Cuts at: 0 3559 3633
Size: 3559 74
PspOMI G'GGCC_C
Cuts at: 0 77 3633
Size: 77 3556
PstI C_TGCA'G
Cuts at: 0 197 1190 1289 1661 1685 1757 3633
Size: 197 993 99 372 24 72 1876
Fragments arranged by size:
1876 993 372 197 99 72 24
PvuI CG_AT'CG
Cuts at: 0 1937 3633
Size: 1937 1696
PvuII CAG'CTG
Cuts at: 0 401 1281 1522 1947 2106 2586 3633
Size: 401 880 241 425 159 480 1047
Fragments arranged by size:
1047 880 480 425 401 241 159
SacI G_AGCT'C
Cuts at: 0 1655 3633
Size: 1655 1978
SacII CC_GC'GG
Cuts at: 0 13 75 3633
Size: 13 62 3558
Fragments arranged by size:
3558 62 13
SapI GCTCTTCn'nnn_
Cuts at: 0 1838 3056 3633
Size: 1838 1218 577
Fragments arranged by size:
1838 1218 577
SexAI A'CCwGG_T
Cuts at: 0 2286 3374 3633
Size: 2286 1088 259
Fragments arranged by size:
2286 1088 259
SfcI C'TryA_G
Cuts at: 0 193 565 1186 1285 1510 1657 1681 1753
Size: 193 372 621 99 225 147 24 72
Cuts at: 1753 3633
Size: 1880
Fragments arranged by size:
1880 621 372 225 193 147 99 72
24
SfoI GGC'GCC
Cuts at: 0 1668 2400 3633
Size: 1668 732 1233
Fragments arranged by size:
1668 1233 732
SgrAI Cr'CCGG_yG
Cuts at: 0 55 3633
Size: 55 3578
SphI G_CATG'C
Cuts at: 0 1929 3222 3633
Size: 1929 1293 411
Fragments arranged by size:
1929 1293 411
StyI C'CwwG_G
Cuts at: 0 3422 3542 3633
Size: 3422 120 91
Fragments arranged by size:
3422 120 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1075 2282 3610 3633
Size: 1075 1207 1328 23
Fragments arranged by size:
1328 1207 1075 23
TatI w'GTAC_w
Cuts at: 0 323 1121 2481 3609 3633
Size: 323 798 1360 1128 24
Fragments arranged by size:
1360 1128 798 323 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 125 157 2903 2935 3418 3450 3633
Size: 125 32 2746 32 483 32 183
Fragments arranged by size:
2746 483 183 125 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 823 1744 3633
Size: 823 921 1889
Fragments arranged by size:
1889 921 823
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 432 3633
Size: 432 3201
XmnI GAAnn'nnTTC
Cuts at: 0 1390 3633
Size: 1390 2243
ZraI GAC'GTC
Cuts at: 0 3517 3633
Size: 3517 116
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspMI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII SacI SacII
SapI SexAI SfcI SfoI SgrAI SphI StyI TaqII
TatI TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BmtI Bpu10I BpuEI BsiWI
BsmBI BspHI BsrBI BsrDI BssHII BstEII BstZ17I ClaI
CspCI DraI DrdI FalI FseI FspAI HindIII HpaI
KpnI MfeI MluI NcoI NheI NotI NruI PacI
PmeI PshAI PspXI PsrI RsrII SalI SanDI SbfI
ScaI SfiI SgfI SmaI SmlI SnaBI SpeI SrfI
SspI StuI SwaI XbaI XhoI XmaI