(Linear) (Six-base) MAPSORT of: KIAA1258.seq  Check: 2767  from: 1  to: 1362



KIAA1258.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1326    1362 
   Size:       1326      36
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1089    1362 
   Size:       1089     273
 
AleI CACnn'nnGTG

Cuts at:      0     787    1362 
   Size:        787     575
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     304     855    1362 
   Size:        304     551     507

  Fragments arranged by size: 

                 551     507     304
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     323     355    1362 
   Size:        323      32    1007

  Fragments arranged by size: 

                1007     323      32
 
ApoI r'AATT_y

Cuts at:      0     475    1180    1362 
   Size:        475     705     182

  Fragments arranged by size: 

                 705     475     182
 
AseI AT'TA_AT

Cuts at:      0    1064    1362 
   Size:       1064     298
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     974    1007    1362 
   Size:        974      33     355

  Fragments arranged by size: 

                 974     355      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1095    1362 
   Size:       1095     267
 
BclI T'GATC_A

Cuts at:      0    1196    1362 
   Size:       1196     166
 
BlpI GC'TnA_GC

Cuts at:      0     922    1362 
   Size:        922     440
 
BmgBI CAC'GTC

Cuts at:      0     990    1362 
   Size:        990     372
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     685     761    1362 
   Size:        685      76     601

  Fragments arranged by size: 

                 685     601      76
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     538    1362 
   Size:        538     824
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      88     896    1362 
   Size:         88     808     466

  Fragments arranged by size: 

                 808     466      88
 
BseYI C'CCAG_C

Cuts at:      0     978    1362 
   Size:        978     384
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      76     296    1326    1362 
   Size:         76     220    1030      36

  Fragments arranged by size: 

                1030     220      76      36
 
BsrBI CCG'CTC

Cuts at:      0      10    1362 
   Size:         10    1352
 
BsrDI GCAATG_nn'

Cuts at:      0     683    1362 
   Size:        683     679
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     506    1362 
   Size:        506     856
 
BtgI C'CryG_G

Cuts at:      0      74    1362 
   Size:         74    1288
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     876    1362 
   Size:        876     486
 
BtsI GCAGTG_nn'

Cuts at:      0     933    1043    1362 
   Size:        933     110     319

  Fragments arranged by size: 

                 933     319     110
 
ClaI AT'CG_AT

Cuts at:      0     559    1362 
   Size:        559     803
 
EaeI y'GGCC_r

Cuts at:      0     161    1362 
   Size:        161    1201
 
EarI CTCTTCn'nnn_

Cuts at:      0    1026    1307    1362 
   Size:       1026     281      55

  Fragments arranged by size: 

                1026     281      55
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1343    1362 
   Size:       1343      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1089    1362 
   Size:       1089     273
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     875     907    1362 
   Size:        875      32     455

  Fragments arranged by size: 

                 875     455      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1238    1362 
   Size:       1238     124
 
MscI TGG'CCA

Cuts at:      0     163    1362 
   Size:        163    1199
 
MslI CAynn'nnrTG

Cuts at:      0      79     248     722     787    1362 
   Size:         79     169     474      65     575

  Fragments arranged by size: 

                 575     474     169      79      65
 
MspA1I CmG'CkG

Cuts at:      0     116     486    1362 
   Size:        116     370     876

  Fragments arranged by size: 

                 876     370     116
 
NcoI C'CATG_G

Cuts at:      0      74    1362 
   Size:         74    1288
 
PacI TTA_AT'TAA

Cuts at:      0    1064    1362 
   Size:       1064     298
 
PasI CC'CwG_GG

Cuts at:      0    1137    1362 
   Size:       1137     225
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     170    1137    1362 
   Size:        170     967     225

  Fragments arranged by size: 

                 967     225     170
 
PshAI GACnn'nnGTC

Cuts at:      0      49    1362 
   Size:         49    1313
 
PsiI TTA'TAA

Cuts at:      0     773    1362 
   Size:        773     589
 
PstI C_TGCA'G

Cuts at:      0     330     855    1362 
   Size:        330     525     507

  Fragments arranged by size: 

                 525     507     330
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1026    1362 
   Size:       1026     336
 
SfcI C'TryA_G

Cuts at:      0     326     851    1362 
   Size:        326     525     511

  Fragments arranged by size: 

                 525     511     326
 
SspI AAT'ATT

Cuts at:      0    1308    1362 
   Size:       1308      54
 
StyI C'CwwG_G

Cuts at:      0      74    1362 
   Size:         74    1288
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     658    1362 
   Size:        658     704
 
TatI w'GTAC_w

Cuts at:      0     314    1362 
   Size:        314    1048
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1322    1354    1362 
   Size:       1322      32       8

  Fragments arranged by size: 

                1322      32       8
 
XbaI T'CTAG_A

Cuts at:      0     610    1362 
   Size:        610     752
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     299    1362 
   Size:        299    1063
 
XmnI GAAnn'nnTTC

Cuts at:      0     868    1362 
   Size:        868     494
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI     AloI     ApoI     AseI     BaeI
     BbsI     BclI     BlpI    BmgBI     BmrI     BsaI    BseRI    BseYI
    BspMI    BsrBI    BsrDI   BstAPI     BtgI    BtgZI     BtsI     ClaI
     EaeI     EarI     EciI  Eco57MI    Hin4I     MmeI     MscI     MslI
   MspA1I     NcoI     PacI     PasI    PflMI    PshAI     PsiI     PstI
     SapI     SfcI     SspI     StyI    TaqII     TatI     TstI     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII Bme1580I     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI  Tth111I     XhoI
     XmaI     ZraI