Restriction map for
pF1KA1261
(Linear) (Six-base) MAPSORT of: KIAA1261.seq Check: 9704 from: 1 to: 2319
KIAA1261.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 345 2319
Size: 345 1974
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 440 599 722 2319
Size: 166 274 159 123 1597
Fragments arranged by size:
1597 274 166 159 123
AflIII A'CryG_T
Cuts at: 0 1500 1502 2319
Size: 1500 2 817
Fragments arranged by size:
1500 817 2
AleI CACnn'nnGTG
Cuts at: 0 1289 2319
Size: 1289 1030
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 74 2319
Size: 74 2245
AlwNI CAG_nnn'CTG
Cuts at: 0 1219 1934 2319
Size: 1219 715 385
Fragments arranged by size:
1219 715 385
ApaLI G'TGCA_C
Cuts at: 0 284 2319
Size: 284 2035
ApoI r'AATT_y
Cuts at: 0 73 2319
Size: 73 2246
AvaI C'yCGr_G
Cuts at: 0 797 2319
Size: 797 1522
BamHI G'GATC_C
Cuts at: 0 783 2319
Size: 783 1536
BanI G'GyrC_C
Cuts at: 0 45 1891 2319
Size: 45 1846 428
Fragments arranged by size:
1846 428 45
BanII G_rGCy'C
Cuts at: 0 464 613 808 1187 2319
Size: 464 149 195 379 1132
Fragments arranged by size:
1132 464 379 195 149
BbsI GAAGACnn'nnnn_
Cuts at: 0 184 2319
Size: 184 2135
BciVI GTATCCnnnnn_n'
Cuts at: 0 630 2319
Size: 630 1689
BclI T'GATC_A
Cuts at: 0 579 2319
Size: 579 1740
BglI GCCn_nnn'nGGC
Cuts at: 0 1553 2319
Size: 1553 766
BglII A'GATC_T
Cuts at: 0 1985 2319
Size: 1985 334
BlpI GC'TnA_GC
Cuts at: 0 2236 2319
Size: 2236 83
Bme1580I G_kGCm'C
Cuts at: 0 288 1133 2319
Size: 288 845 1186
Fragments arranged by size:
1186 845 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 448 501 2033 2202 2319
Size: 382 66 53 1532 169 117
Fragments arranged by size:
1532 382 169 117 66 53
Bpu10I CC'TnA_GC
Cuts at: 0 13 2319
Size: 13 2306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 1056 2104 2319
Size: 838 218 1048 215
Fragments arranged by size:
1048 838 218 215
BsaI GGTCTCn'nnnn_
Cuts at: 0 433 2319
Size: 433 1886
BsaAI yAC'GTr
Cuts at: 0 1503 2319
Size: 1503 816
BsaHI Gr'CG_yC
Cuts at: 0 1400 2319
Size: 1400 919
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 413 443 583 613 646 676 963 993
Size: 413 30 140 30 33 30 287 30
Cuts at: 993 1180 1210 1453 1483 1963 1993 2319
Size: 187 30 243 30 480 30 326
Fragments arranged by size:
480 413 326 287 243 187 140 33
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 666 900 2319
Size: 666 234 1419
Fragments arranged by size:
1419 666 234
BseYI C'CCAG_C
Cuts at: 0 404 807 1180 1574 2319
Size: 404 403 373 394 745
Fragments arranged by size:
745 404 403 394 373
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 1801 2319
Size: 352 1449 518
Fragments arranged by size:
1449 518 352
BsiHKAI G_wGCw'C
Cuts at: 0 288 317 525 613 2319
Size: 288 29 208 88 1706
Fragments arranged by size:
1706 288 208 88 29
BsmI GAATG_Cn'
Cuts at: 0 279 1322 2181 2319
Size: 279 1043 859 138
Fragments arranged by size:
1043 859 279 138
BsmBI CGTCTCn'nnnn_
Cuts at: 0 963 2319
Size: 963 1356
Bsp1286I G_dGCh'C
Cuts at: 0 288 317 464 525 613 808 1133 1187
Size: 288 29 147 61 88 195 325 54
Cuts at: 1187 2319
Size: 1132
Fragments arranged by size:
1132 325 288 195 147 88 61 54
29
BspHI T'CATG_A
Cuts at: 0 2097 2319
Size: 2097 222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 345 1949 2319
Size: 345 1604 370
Fragments arranged by size:
1604 370 345
BsrBI CCG'CTC
Cuts at: 0 1171 2319
Size: 1171 1148
BsrDI GCAATG_nn'
Cuts at: 0 2052 2319
Size: 2052 267
BsrGI T'GTAC_A
Cuts at: 0 1505 2319
Size: 1505 814
BssSI C'ACGA_G
Cuts at: 0 1494 2319
Size: 1494 825
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1219 2319
Size: 1219 1100
BstEII G'GTnAC_C
Cuts at: 0 357 1476 2319
Size: 357 1119 843
Fragments arranged by size:
1119 843 357
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1858 2319
Size: 1858 461
BstYI r'GATC_y
Cuts at: 0 783 1812 1985 2319
Size: 783 1029 173 334
Fragments arranged by size:
1029 783 334 173
Bsu36I CC'TnA_GG
Cuts at: 0 1078 2319
Size: 1078 1241
BtgI C'CryG_G
Cuts at: 0 362 1455 2252 2319
Size: 362 1093 797 67
Fragments arranged by size:
1093 797 362 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 749 1785 2319
Size: 749 1036 534
Fragments arranged by size:
1036 749 534
BtsI GCAGTG_nn'
Cuts at: 0 501 2033 2319
Size: 501 1532 286
Fragments arranged by size:
1532 501 286
DraI TTT'AAA
Cuts at: 0 72 2319
Size: 72 2247
DrdI GACnn_nn'nnGTC
Cuts at: 0 1924 2319
Size: 1924 395
EaeI y'GGCC_r
Cuts at: 0 1748 2319
Size: 1748 571
EarI CTCTTCn'nnn_
Cuts at: 0 104 618 2319
Size: 104 514 1701
Fragments arranged by size:
1701 514 104
EcoICRI GAG'CTC
Cuts at: 0 611 2319
Size: 611 1708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 382 440 448 501 599 722 2033
Size: 166 216 58 8 53 98 123 1311
Cuts at: 2033 2202 2319
Size: 169 117
Fragments arranged by size:
1311 216 169 166 123 117 98 58
53 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1833 1945 2319
Size: 1833 112 374
Fragments arranged by size:
1833 374 112
EcoO109I rG'GnC_Cy
Cuts at: 0 1082 1680 1931 1938 2319
Size: 1082 598 251 7 381
Fragments arranged by size:
1082 598 381 251 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1791 2319
Size: 1791 528
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 413 445 583 587 615 619 661 693
Size: 413 32 138 4 28 4 42 32
Cuts at: 693 772 804 1403 1435 1705 1737 1963 1977
Size: 79 32 599 32 270 32 226 14
Cuts at: 1977 1995 2009 2319
Size: 18 14 310
Fragments arranged by size:
599 413 310 270 226 138 79 42
32 32 32 32 32 28 18 14
14 4 4
HincII GTy'rAC
Cuts at: 0 768 1059 2319
Size: 768 291 1260
Fragments arranged by size:
1260 768 291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1135 1629 1717 2319
Size: 1135 494 88 602
Fragments arranged by size:
1135 602 494 88
MscI TGG'CCA
Cuts at: 0 1750 2319
Size: 1750 569
MslI CAynn'nnrTG
Cuts at: 0 422 575 1289 1291 1804 1856 2096 2251
Size: 422 153 714 2 513 52 240 155
Cuts at: 2251 2319
Size: 68
Fragments arranged by size:
714 513 422 240 155 153 68 52
2
MspA1I CmG'CkG
Cuts at: 0 192 1216 1231 1868 1959 2319
Size: 192 1024 15 637 91 360
Fragments arranged by size:
1024 637 360 192 91 15
NcoI C'CATG_G
Cuts at: 0 362 2319
Size: 362 1957
NruI TCG'CGA
Cuts at: 0 8 2319
Size: 8 2311
NspI r_CATG'y
Cuts at: 0 1290 1432 2065 2319
Size: 1290 142 633 254
Fragments arranged by size:
1290 633 254 142
PflMI CCAn_nnn'nTGG
Cuts at: 0 1830 1901 2319
Size: 1830 71 418
Fragments arranged by size:
1830 418 71
PfoI T'CCnGG_A
Cuts at: 0 1020 1326 2319
Size: 1020 306 993
Fragments arranged by size:
1020 993 306
PmlI CAC'GTG
Cuts at: 0 1503 2319
Size: 1503 816
PpuMI rG'GwC_Cy
Cuts at: 0 1082 1680 1931 1938 2319
Size: 1082 598 251 7 381
Fragments arranged by size:
1082 598 381 251 7
PspXI vC'TCGA_Gb
Cuts at: 0 797 2319
Size: 797 1522
PstI C_TGCA'G
Cuts at: 0 1618 1789 1964 2319
Size: 1618 171 175 355
Fragments arranged by size:
1618 355 175 171
PvuII CAG'CTG
Cuts at: 0 1216 1868 1959 2319
Size: 1216 652 91 360
Fragments arranged by size:
1216 652 360 91
SacI G_AGCT'C
Cuts at: 0 613 2319
Size: 613 1706
SapI GCTCTTCn'nnn_
Cuts at: 0 618 2319
Size: 618 1701
ScaI AGT'ACT
Cuts at: 0 903 1665 2319
Size: 903 762 654
Fragments arranged by size:
903 762 654
SfcI C'TryA_G
Cuts at: 0 1614 1785 1960 2319
Size: 1614 171 175 359
Fragments arranged by size:
1614 359 175 171
SmlI C'TyrA_G
Cuts at: 0 797 853 1071 2119 2319
Size: 797 56 218 1048 200
Fragments arranged by size:
1048 797 218 200 56
SphI G_CATG'C
Cuts at: 0 1432 2319
Size: 1432 887
StuI AGG'CCT
Cuts at: 0 1032 2319
Size: 1032 1287
StyI C'CwwG_G
Cuts at: 0 362 1766 1829 2319
Size: 362 1404 63 490
Fragments arranged by size:
1404 490 362 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 498 1565 2319
Size: 498 1067 754
Fragments arranged by size:
1067 754 498
TatI w'GTAC_w
Cuts at: 0 901 1505 1663 2319
Size: 901 604 158 656
Fragments arranged by size:
901 656 604 158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 646 678 1180 1212 1453 1485 2319
Size: 646 32 502 32 241 32 834
Fragments arranged by size:
834 646 502 241 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 425 2319
Size: 425 1894
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1680 2015 2319
Size: 1680 335 304
Fragments arranged by size:
1680 335 304
XhoI C'TCGA_G
Cuts at: 0 797 2319
Size: 797 1522
XmnI GAAnn'nnTTC
Cuts at: 0 113 2319
Size: 113 2206
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaLI ApoI
AvaI BamHI BanI BanII BbsI BciVI BclI BglI
BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
Hin4I HincII MmeI MscI MslI MspA1I NcoI NruI
NspI PflMI PfoI PmlI PpuMI PspXI PstI PvuII
SacI SapI ScaI SfcI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BfrBI BmgBI BmrI BmtI BplI BsaBI
BsaWI BsiEI BsiWI BspEI BsrFI BssHII BstBI BstZ17I
ClaI CspCI DraIII EagI EciI EcoRI EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NsiI
PacI PasI PciI PmeI PpiI PshAI PsiI PspOMI
PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI XbaI XmaI ZraI