Restriction map for
pF1KSDA1263
(Linear) (Six-base) MAPSORT of: KIAA1263.seq Check: 9 from: 1 to: 2538
KIAA1263.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 964 2538
Size: 964 1574
Acc65I G'GTAC_C
Cuts at: 0 1681 2538
Size: 1681 857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2264 2516 2538
Size: 2264 252 22
Fragments arranged by size:
2264 252 22
AflIII A'CryG_T
Cuts at: 0 1352 2538
Size: 1352 1186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 362 2538
Size: 362 2176
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1472 1504 2538
Size: 1472 32 1034
Fragments arranged by size:
1472 1034 32
AlwNI CAG_nnn'CTG
Cuts at: 0 778 1621 2538
Size: 778 843 917
Fragments arranged by size:
917 843 778
ApaLI G'TGCA_C
Cuts at: 0 337 2538
Size: 337 2201
ApoI r'AATT_y
Cuts at: 0 313 706 1408 2538
Size: 313 393 702 1130
Fragments arranged by size:
1130 702 393 313
AseI AT'TA_AT
Cuts at: 0 587 2123 2538
Size: 587 1536 415
Fragments arranged by size:
1536 587 415
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1218 1251 2538
Size: 1218 33 1287
Fragments arranged by size:
1287 1218 33
BanI G'GyrC_C
Cuts at: 0 1079 1681 2538
Size: 1079 602 857
Fragments arranged by size:
1079 857 602
BanII G_rGCy'C
Cuts at: 0 2309 2538
Size: 2309 229
BbsI GAAGACnn'nnnn_
Cuts at: 0 222 395 887 1271 1295 1699 2538
Size: 222 173 492 384 24 404 839
Fragments arranged by size:
839 492 404 384 222 173 24
BbvCI CC'TCA_GC
Cuts at: 0 1106 1475 2538
Size: 1106 369 1063
Fragments arranged by size:
1106 1063 369
BciVI GTATCCnnnnn_n'
Cuts at: 0 1043 2538
Size: 1043 1495
BclI T'GATC_A
Cuts at: 0 1656 2538
Size: 1656 882
BlpI GC'TnA_GC
Cuts at: 0 1672 2538
Size: 1672 866
Bme1580I G_kGCm'C
Cuts at: 0 341 2083 2538
Size: 341 1742 455
Fragments arranged by size:
1742 455 341
BmrI ACTGGGnnnn_n'
Cuts at: 0 1621 2538
Size: 1621 917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1001 1241 2081 2538
Size: 67 934 240 840 457
Fragments arranged by size:
934 840 457 240 67
Bpu10I CC'TnA_GC
Cuts at: 0 1106 1475 2538
Size: 1106 369 1063
Fragments arranged by size:
1106 1063 369
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2326 2338 2538
Size: 2326 12 200
Fragments arranged by size:
2326 200 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 819 2538
Size: 819 1719
BsaWI w'CCGG_w
Cuts at: 0 2005 2378 2538
Size: 2005 373 160
Fragments arranged by size:
2005 373 160
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1472 1502 2067 2097 2538
Size: 1472 30 565 30 441
Fragments arranged by size:
1472 565 441 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2183 2538
Size: 2183 355
BseYI C'CCAG_C
Cuts at: 0 1676 2538
Size: 1676 862
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1603 1853 2191 2538
Size: 1603 250 338 347
Fragments arranged by size:
1603 347 338 250
BsiHKAI G_wGCw'C
Cuts at: 0 341 1615 2297 2309 2538
Size: 341 1274 682 12 229
Fragments arranged by size:
1274 682 341 229 12
BsmI GAATG_Cn'
Cuts at: 0 718 2538
Size: 718 1820
Bsp1286I G_dGCh'C
Cuts at: 0 341 1615 2083 2297 2309 2538
Size: 341 1274 468 214 12 229
Fragments arranged by size:
1274 468 341 229 214 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 964 2538
Size: 964 1574
BsrDI GCAATG_nn'
Cuts at: 0 1206 2538
Size: 1206 1332
BstAPI GCAn_nnn'nTGC
Cuts at: 0 256 2538
Size: 256 2282
BstEII G'GTnAC_C
Cuts at: 0 927 2538
Size: 927 1611
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2404 2538
Size: 2404 134
BstYI r'GATC_y
Cuts at: 0 184 301 630 2538
Size: 184 117 329 1908
Fragments arranged by size:
1908 329 184 117
BtsI GCAGTG_nn'
Cuts at: 0 767 1188 1613 2538
Size: 767 421 425 925
Fragments arranged by size:
925 767 425 421
DraI TTT'AAA
Cuts at: 0 402 549 2538
Size: 402 147 1989
Fragments arranged by size:
1989 402 147
EaeI y'GGCC_r
Cuts at: 0 1724 2103 2538
Size: 1724 379 435
Fragments arranged by size:
1724 435 379
EarI CTCTTCn'nnn_
Cuts at: 0 1278 2316 2455 2538
Size: 1278 1038 139 83
Fragments arranged by size:
1278 1038 139 83
EcoICRI GAG'CTC
Cuts at: 0 2307 2538
Size: 2307 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1001 1241 2081 2264 2516 2538
Size: 67 934 240 840 183 252 22
Fragments arranged by size:
934 840 252 240 183 67 22
EcoO109I rG'GnC_Cy
Cuts at: 0 908 2538
Size: 908 1630
EcoRI G'AATT_C
Cuts at: 0 313 2538
Size: 313 2225
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 651 2538
Size: 651 1887
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 499 531 892 924 1570 1602 1681 1713
Size: 499 32 361 32 646 32 79 32
Cuts at: 1713 2067 2099 2322 2354 2538
Size: 354 32 223 32 184
Fragments arranged by size:
646 499 361 354 223 184 79 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1147 2538
Size: 1147 1391
KpnI G_GTAC'C
Cuts at: 0 1685 2538
Size: 1685 853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 2508 2538
Size: 32 2476 30
Fragments arranged by size:
2476 32 30
MscI TGG'CCA
Cuts at: 0 1726 2105 2538
Size: 1726 379 433
Fragments arranged by size:
1726 433 379
MslI CAynn'nnrTG
Cuts at: 0 1027 1831 1859 2538
Size: 1027 804 28 679
Fragments arranged by size:
1027 804 679 28
MspA1I CmG'CkG
Cuts at: 0 1513 2538
Size: 1513 1025
NdeI CA'TA_TG
Cuts at: 0 1832 2088 2538
Size: 1832 256 450
Fragments arranged by size:
1832 450 256
NspI r_CATG'y
Cuts at: 0 1356 2538
Size: 1356 1182
PciI A'CATG_T
Cuts at: 0 1352 2538
Size: 1352 1186
PflMI CCAn_nnn'nTGG
Cuts at: 0 839 1767 2538
Size: 839 928 771
Fragments arranged by size:
928 839 771
PfoI T'CCnGG_A
Cuts at: 0 2396 2538
Size: 2396 142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1472 1504 2538
Size: 1472 32 1034
Fragments arranged by size:
1472 1034 32
PpuMI rG'GwC_Cy
Cuts at: 0 908 2538
Size: 908 1630
PstI C_TGCA'G
Cuts at: 0 774 2538
Size: 774 1764
PvuII CAG'CTG
Cuts at: 0 1513 2538
Size: 1513 1025
SacI G_AGCT'C
Cuts at: 0 2309 2538
Size: 2309 229
ScaI AGT'ACT
Cuts at: 0 202 2538
Size: 202 2336
SfcI C'TryA_G
Cuts at: 0 770 1708 2538
Size: 770 938 830
Fragments arranged by size:
938 830 770
SmlI C'TyrA_G
Cuts at: 0 2341 2353 2538
Size: 2341 12 185
Fragments arranged by size:
2341 185 12
SspI AAT'ATT
Cuts at: 0 553 855 2538
Size: 553 302 1683
Fragments arranged by size:
1683 553 302
StyI C'CwwG_G
Cuts at: 0 1443 1685 2371 2438 2538
Size: 1443 242 686 67 100
Fragments arranged by size:
1443 686 242 100 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1656 1774 1843 2538
Size: 1656 118 69 695
Fragments arranged by size:
1656 695 118 69
TatI w'GTAC_w
Cuts at: 0 200 643 2153 2538
Size: 200 443 1510 385
Fragments arranged by size:
1510 443 385 200
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1370 1402 1918 1950 2538
Size: 1370 32 516 32 588
Fragments arranged by size:
1370 588 516 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1564 1621 2050 2538
Size: 1564 57 429 488
Fragments arranged by size:
1564 488 429 57
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1837 2538
Size: 1837 701
XmnI GAAnn'nnTTC
Cuts at: 0 1354 2538
Size: 1354 1184
Enzymes that do cut:
AarI Acc65I AcuI AflIII AlfI AloI AlwNI ApaLI
ApoI AseI BaeI BanI BanII BbsI BbvCI BciVI
BclI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BstAPI BstEII BstXI BstYI BtsI DraI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I
HindIII KpnI MmeI MscI MslI MspA1I NdeI NspI
PciI PflMI PfoI PpiI PpuMI PstI PvuII SacI
ScaI SfcI SmlI SspI StyI TaqII TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
ApaI AscI AsiSI AvaI AvrII BamHI BbeI BcgI
BfrBI BglI BglII BmgBI BmtI BplI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraIII DrdI EagI EciI EcoNI EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI MfeI
MluI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI XbaI XhoI XmaI
ZraI