Restriction map for
pF1KSDA1264
(Linear) (Six-base) MAPSORT of: KIAA1264.seq Check: 346 from: 1 to: 2157
KIAA1264.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
Acc65I G'GTAC_C
Cuts at: 0 1127 2157
Size: 1127 1030
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1994 2157
Size: 1994 163
AflIII A'CryG_T
Cuts at: 0 1523 2157
Size: 1523 634
AhdI GACnn_n'nnGTC
Cuts at: 0 1878 2157
Size: 1878 279
AlwNI CAG_nnn'CTG
Cuts at: 0 1039 2157
Size: 1039 1118
ApaI G_GGCC'C
Cuts at: 0 1060 2157
Size: 1060 1097
AvaI C'yCGr_G
Cuts at: 0 666 867 1885 2157
Size: 666 201 1018 272
Fragments arranged by size:
1018 666 272 201
BamHI G'GATC_C
Cuts at: 0 468 1961 2157
Size: 468 1493 196
Fragments arranged by size:
1493 468 196
BanI G'GyrC_C
Cuts at: 0 1127 1585 1788 2157
Size: 1127 458 203 369
Fragments arranged by size:
1127 458 369 203
BanII G_rGCy'C
Cuts at: 0 971 1060 1207 1252 1556 1665 1922 2054
Size: 971 89 147 45 304 109 257 132
Cuts at: 2054 2157
Size: 103
Fragments arranged by size:
971 304 257 147 132 109 103 89
45
BbsI GAAGACnn'nnnn_
Cuts at: 0 208 800 2157
Size: 208 592 1357
Fragments arranged by size:
1357 592 208
BbvCI CC'TCA_GC
Cuts at: 0 833 1018 1210 2157
Size: 833 185 192 947
Fragments arranged by size:
947 833 192 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 394 428 2157
Size: 394 34 1729
Fragments arranged by size:
1729 394 34
BclI T'GATC_A
Cuts at: 0 1487 2157
Size: 1487 670
BfrBI ATG'CAT
Cuts at: 0 173 1391 2157
Size: 173 1218 766
Fragments arranged by size:
1218 766 173
Bme1580I G_kGCm'C
Cuts at: 0 512 1060 2157
Size: 512 548 1097
Fragments arranged by size:
1097 548 512
BmrI ACTGGGnnnn_n'
Cuts at: 0 77 381 2157
Size: 77 304 1776
Fragments arranged by size:
1776 304 77
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 859 1257 1930 2157
Size: 321 538 398 673 227
Fragments arranged by size:
673 538 398 321 227
Bpu10I CC'TnA_GC
Cuts at: 0 833 1018 1201 1210 2157
Size: 833 185 183 9 947
Fragments arranged by size:
947 833 185 183 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 1086 1757 2157
Size: 628 458 671 400
Fragments arranged by size:
671 628 458 400
BsaI GGTCTCn'nnnn_
Cuts at: 0 1322 2157
Size: 1322 835
BsaAI yAC'GTr
Cuts at: 0 513 2157
Size: 513 1644
BsaBI GATnn'nnATC
Cuts at: 0 467 998 2157
Size: 467 531 1159
Fragments arranged by size:
1159 531 467
BsaWI w'CCGG_w
Cuts at: 0 1445 2157
Size: 1445 712
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1189 1219 2157
Size: 1189 30 938
Fragments arranged by size:
1189 938 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1242 1245 2071 2157
Size: 1242 3 826 86
Fragments arranged by size:
1242 826 86 3
BseYI C'CCAG_C
Cuts at: 0 107 775 1240 1244 2157
Size: 107 668 465 4 913
Fragments arranged by size:
913 668 465 107 4
BsiEI CG_ry'CG
Cuts at: 0 428 1002 2157
Size: 428 574 1155
Fragments arranged by size:
1155 574 428
BsiHKAI G_wGCw'C
Cuts at: 0 736 1008 1207 1556 1665 1773 2018 2157
Size: 736 272 199 349 109 108 245 139
Fragments arranged by size:
736 349 272 245 199 139 109 108
BsmI GAATG_Cn'
Cuts at: 0 980 2157
Size: 980 1177
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1452 2157
Size: 1452 705
Bsp1286I G_dGCh'C
Cuts at: 0 512 736 971 1008 1060 1207 1252 1556
Size: 512 224 235 37 52 147 45 304
Cuts at: 1556 1665 1773 1922 2018 2054 2157
Size: 109 108 149 96 36 103
Fragments arranged by size:
512 304 235 224 149 147 109 108
103 96 52 45 37 36
BspEI T'CCGG_A
Cuts at: 0 1445 2157
Size: 1445 712
BspHI T'CATG_A
Cuts at: 0 2135 2157
Size: 2135 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2098 2157
Size: 2098 59
BsrFI r'CCGG_y
Cuts at: 0 97 1378 2157
Size: 97 1281 779
Fragments arranged by size:
1281 779 97
BsrGI T'GTAC_A
Cuts at: 0 1526 2157
Size: 1526 631
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1388 2157
Size: 1388 769
BstXI CCAn_nnnn'nTGG
Cuts at: 0 189 2157
Size: 189 1968
BstYI r'GATC_y
Cuts at: 0 468 1961 2157
Size: 468 1493 196
Fragments arranged by size:
1493 468 196
Bsu36I CC'TnA_GG
Cuts at: 0 298 1835 2157
Size: 298 1537 322
Fragments arranged by size:
1537 322 298
BtgI C'CryG_G
Cuts at: 0 342 1187 2157
Size: 342 845 970
Fragments arranged by size:
970 845 342
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1377 1562 1748 1966 2157
Size: 1377 185 186 218 191
Fragments arranged by size:
1377 218 191 186 185
BtsI GCAGTG_nn'
Cuts at: 0 118 1433 2157
Size: 118 1315 724
Fragments arranged by size:
1315 724 118
DraI TTT'AAA
Cuts at: 0 912 2157
Size: 912 1245
DraIII CAC_nnn'GTG
Cuts at: 0 977 2157
Size: 977 1180
EaeI y'GGCC_r
Cuts at: 0 137 1346 1737 2157
Size: 137 1209 391 420
Fragments arranged by size:
1209 420 391 137
EarI CTCTTCn'nnn_
Cuts at: 0 439 1789 2157
Size: 439 1350 368
Fragments arranged by size:
1350 439 368
EcoICRI GAG'CTC
Cuts at: 0 1205 1554 1663 2157
Size: 1205 349 109 494
Fragments arranged by size:
1205 494 349 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 859 1257 1930 1994 2157
Size: 321 538 398 673 64 163
Fragments arranged by size:
673 538 398 321 163 64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 256 1050 1999 2157
Size: 256 794 949 158
Fragments arranged by size:
949 794 256 158
EcoO109I rG'GnC_Cy
Cuts at: 0 772 1056 1057 1992 2157
Size: 772 284 1 935 165
Fragments arranged by size:
935 772 284 165 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 583 615 816 848 1864 1896
Size: 259 32 292 32 201 32 1016 32
Cuts at: 1896 2157
Size: 261
Fragments arranged by size:
1016 292 261 259 201 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1521 2157
Size: 1521 636
KpnI G_GTAC'C
Cuts at: 0 1131 2157
Size: 1131 1026
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 314 1283 2157
Size: 68 246 969 874
Fragments arranged by size:
969 874 246 68
MscI TGG'CCA
Cuts at: 0 1348 2157
Size: 1348 809
MslI CAynn'nnrTG
Cuts at: 0 277 1511 1615 2157
Size: 277 1234 104 542
Fragments arranged by size:
1234 542 277 104
MspA1I CmG'CkG
Cuts at: 0 189 728 1244 1311 2157
Size: 189 539 516 67 846
Fragments arranged by size:
846 539 516 189 67
NsiI A_TGCA'T
Cuts at: 0 175 1393 2157
Size: 175 1218 764
Fragments arranged by size:
1218 764 175
NspI r_CATG'y
Cuts at: 0 1527 2157
Size: 1527 630
PasI CC'CwG_GG
Cuts at: 0 1052 1334 2157
Size: 1052 282 823
Fragments arranged by size:
1052 823 282
PciI A'CATG_T
Cuts at: 0 1523 2157
Size: 1523 634
PfoI T'CCnGG_A
Cuts at: 0 875 1964 2157
Size: 875 1089 193
Fragments arranged by size:
1089 875 193
PpuMI rG'GwC_Cy
Cuts at: 0 772 1992 2157
Size: 772 1220 165
Fragments arranged by size:
1220 772 165
PspOMI G'GGCC_C
Cuts at: 0 1056 2157
Size: 1056 1101
PspXI vC'TCGA_Gb
Cuts at: 0 666 2157
Size: 666 1491
PstI C_TGCA'G
Cuts at: 0 720 1166 1310 2157
Size: 720 446 144 847
Fragments arranged by size:
847 720 446 144
PvuI CG_AT'CG
Cuts at: 0 1002 2157
Size: 1002 1155
PvuII CAG'CTG
Cuts at: 0 189 1244 1311 2157
Size: 189 1055 67 846
Fragments arranged by size:
1055 846 189 67
SacI G_AGCT'C
Cuts at: 0 1207 1556 1665 2157
Size: 1207 349 109 492
Fragments arranged by size:
1207 492 349 109
SanDI GG'GwC_CC
Cuts at: 0 772 2157
Size: 772 1385
SfcI C'TryA_G
Cuts at: 0 716 1162 1306 2157
Size: 716 446 144 851
Fragments arranged by size:
851 716 446 144
SmlI C'TyrA_G
Cuts at: 0 643 666 1101 1772 2157
Size: 643 23 435 671 385
Fragments arranged by size:
671 643 435 385 23
StuI AGG'CCT
Cuts at: 0 538 2157
Size: 538 1619
StyI C'CwwG_G
Cuts at: 0 737 924 1683 2157
Size: 737 187 759 474
Fragments arranged by size:
759 737 474 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 195 330 442 1548 2157
Size: 195 135 112 1106 609
Fragments arranged by size:
1106 609 195 135 112
TatI w'GTAC_w
Cuts at: 0 434 1178 1526 2157
Size: 434 744 348 631
Fragments arranged by size:
744 631 434 348
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 998 1030 2157
Size: 998 32 1127
Fragments arranged by size:
1127 998 32
XbaI T'CTAG_A
Cuts at: 0 709 1575 2157
Size: 709 866 582
Fragments arranged by size:
866 709 582
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 744 1120 1341 2157
Size: 744 376 221 816
Fragments arranged by size:
816 744 376 221
XhoI C'TCGA_G
Cuts at: 0 666 2157
Size: 666 1491
XmnI GAAnn'nnTTC
Cuts at: 0 1292 2157
Size: 1292 865
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AlwNI ApaI AvaI BamHI
BanI BanII BbsI BbvCI BcgI BclI BfrBI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrFI BsrGI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII KpnI MmeI
MscI MslI MspA1I NsiI NspI PasI PciI PfoI
PpuMI PspOMI PspXI PstI PvuI PvuII SacI SanDI
SfcI SmlI StuI StyI TaqII TatI TstI XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BciVI BglI BglII BlpI BmgBI BmtI
BplI BsaHI BsgI BsiWI BsrBI BsrDI BssHII BssSI
BstBI BstEII BstZ17I ClaI CspCI DrdI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PflMI PmeI PmlI
PpiI PshAI PsiI PsrI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XmaI
ZraI