Restriction map for
pF1KSDA1266
(Linear) (Six-base) MAPSORT of: KIAA1266.seq Check: 7649 from: 1 to: 1611
KIAA1266.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1611
Size: 674 937
AflIII A'CryG_T
Cuts at: 0 764 1151 1611
Size: 764 387 460
Fragments arranged by size:
764 460 387
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 518 1611
Size: 518 1093
AlwNI CAG_nnn'CTG
Cuts at: 0 652 820 1611
Size: 652 168 791
Fragments arranged by size:
791 652 168
ApaI G_GGCC'C
Cuts at: 0 1012 1611
Size: 1012 599
ApoI r'AATT_y
Cuts at: 0 1258 1611
Size: 1258 353
AseI AT'TA_AT
Cuts at: 0 1328 1611
Size: 1328 283
AvaI C'yCGr_G
Cuts at: 0 518 1611
Size: 518 1093
BamHI G'GATC_C
Cuts at: 0 378 1611
Size: 378 1233
BanII G_rGCy'C
Cuts at: 0 459 1012 1611
Size: 459 553 599
Fragments arranged by size:
599 553 459
BbsI GAAGACnn'nnnn_
Cuts at: 0 1222 1611
Size: 1222 389
BciVI GTATCCnnnnn_n'
Cuts at: 0 207 540 1611
Size: 207 333 1071
Fragments arranged by size:
1071 333 207
BglI GCCn_nnn'nGGC
Cuts at: 0 1345 1611
Size: 1345 266
Bme1580I G_kGCm'C
Cuts at: 0 1012 1611
Size: 1012 599
BmrI ACTGGGnnnn_n'
Cuts at: 0 1083 1202 1611
Size: 1083 119 409
Fragments arranged by size:
1083 409 119
BmtI G_CTAG'C
Cuts at: 0 662 1611
Size: 662 949
BsaAI yAC'GTr
Cuts at: 0 433 1152 1595 1611
Size: 433 719 443 16
Fragments arranged by size:
719 443 433 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 599 628 629 658 948 978 1611
Size: 599 29 1 29 290 30 633
Fragments arranged by size:
633 599 290 30 29 29 1
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 1611
Size: 177 1434
BsiEI CG_ry'CG
Cuts at: 0 1315 1611
Size: 1315 296
BsmI GAATG_Cn'
Cuts at: 0 1181 1544 1611
Size: 1181 363 67
Fragments arranged by size:
1181 363 67
Bsp1286I G_dGCh'C
Cuts at: 0 459 1012 1611
Size: 459 553 599
Fragments arranged by size:
599 553 459
BssSI C'ACGA_G
Cuts at: 0 1136 1611
Size: 1136 475
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1176 1354 1611
Size: 1176 178 257
Fragments arranged by size:
1176 257 178
BstXI CCAn_nnnn'nTGG
Cuts at: 0 459 1611
Size: 459 1152
BstYI r'GATC_y
Cuts at: 0 378 1442 1611
Size: 378 1064 169
Fragments arranged by size:
1064 378 169
BtsI GCAGTG_nn'
Cuts at: 0 165 585 1030 1217 1611
Size: 165 420 445 187 394
Fragments arranged by size:
445 420 394 187 165
EaeI y'GGCC_r
Cuts at: 0 1312 1611
Size: 1312 299
EagI C'GGCC_G
Cuts at: 0 1312 1611
Size: 1312 299
EarI CTCTTCn'nnn_
Cuts at: 0 665 1094 1611
Size: 665 429 517
Fragments arranged by size:
665 517 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1611
Size: 674 937
EcoNI CCTnn'n_nnAGG
Cuts at: 0 950 1611
Size: 950 661
EcoO109I rG'GnC_Cy
Cuts at: 0 289 610 1008 1131 1611
Size: 289 321 398 123 480
Fragments arranged by size:
480 398 321 289 123
EcoRV GAT'ATC
Cuts at: 0 298 1611
Size: 298 1313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 739 1611
Size: 739 872
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 169 201 628 660 1093 1125
Size: 22 32 115 32 427 32 433 32
Cuts at: 1125 1611
Size: 486
Fragments arranged by size:
486 433 427 115 32 32 32 32
22
HindIII A'AGCT_T
Cuts at: 0 1226 1611
Size: 1226 385
MslI CAynn'nnrTG
Cuts at: 0 500 566 1574 1611
Size: 500 66 1008 37
Fragments arranged by size:
1008 500 66 37
NdeI CA'TA_TG
Cuts at: 0 497 1611
Size: 497 1114
NheI G'CTAG_C
Cuts at: 0 658 1611
Size: 658 953
NspI r_CATG'y
Cuts at: 0 19 768 1369 1424 1611
Size: 19 749 601 55 187
Fragments arranged by size:
749 601 187 55 19
PciI A'CATG_T
Cuts at: 0 764 1611
Size: 764 847
PmlI CAC'GTG
Cuts at: 0 433 1152 1595 1611
Size: 433 719 443 16
Fragments arranged by size:
719 443 433 16
PpuMI rG'GwC_Cy
Cuts at: 0 289 610 1131 1611
Size: 289 321 521 480
Fragments arranged by size:
521 480 321 289
PspOMI G'GGCC_C
Cuts at: 0 1008 1611
Size: 1008 603
PstI C_TGCA'G
Cuts at: 0 513 1212 1611
Size: 513 699 399
Fragments arranged by size:
699 513 399
SfcI C'TryA_G
Cuts at: 0 509 1124 1208 1563 1611
Size: 509 615 84 355 48
Fragments arranged by size:
615 509 355 84 48
SpeI A'CTAG_T
Cuts at: 0 883 1611
Size: 883 728
SphI G_CATG'C
Cuts at: 0 19 1611
Size: 19 1592
SspI AAT'ATT
Cuts at: 0 757 1611
Size: 757 854
StyI C'CwwG_G
Cuts at: 0 727 1611
Size: 727 884
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 82 1102 1611
Size: 82 1020 509
Fragments arranged by size:
1020 509 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 885 917 948 980 1182 1214 1611
Size: 885 32 31 32 202 32 397
Fragments arranged by size:
885 397 202 32 32 32 31
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI ApoI AseI AvaI
BamHI BanII BbsI BciVI BglI Bme1580I BmrI BmtI
BsaAI BsaXI BsgI BsiEI BsmI Bsp1286I BssSI BstAPI
BstXI BstYI BtsI EaeI EagI EarI Eco57MI EcoNI
EcoO109I EcoRV FalI Hin4I HindIII MslI NdeI NheI
NspI PciI PmlI PpuMI PspOMI PstI SfcI SpeI
SphI SspI StyI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AsiSI AvrII BaeI
BanI BbeI BbvCI BcgI BclI BfrBI BglII BlpI
BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BseRI BseYI BsiHKAI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EciI EcoICRI EcoRI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
MspA1I NaeI NarI NcoI NgoMIV NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SrfI StuI SwaI TatI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI