Restriction map for
pF1KSDA1269
(Linear) (Six-base) MAPSORT of: KIAA1269.seq Check: 8989 from: 1 to: 1590
KIAA1269.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 529 1110 1590
Size: 529 581 480
Fragments arranged by size:
581 529 480
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 436 739 1018 1228 1590
Size: 260 176 303 279 210 362
Fragments arranged by size:
362 303 279 260 210 176
AflIII A'CryG_T
Cuts at: 0 1349 1590
Size: 1349 241
AleI CACnn'nnGTG
Cuts at: 0 64 1498 1590
Size: 64 1434 92
Fragments arranged by size:
1434 92 64
AlwNI CAG_nnn'CTG
Cuts at: 0 768 1519 1590
Size: 768 751 71
Fragments arranged by size:
768 751 71
ApaI G_GGCC'C
Cuts at: 0 1453 1590
Size: 1453 137
ApaLI G'TGCA_C
Cuts at: 0 22 391 1590
Size: 22 369 1199
Fragments arranged by size:
1199 369 22
ApoI r'AATT_y
Cuts at: 0 385 1590
Size: 385 1205
AvaI C'yCGr_G
Cuts at: 0 938 1155 1282 1525 1590
Size: 938 217 127 243 65
Fragments arranged by size:
938 243 217 127 65
BanI G'GyrC_C
Cuts at: 0 1248 1590
Size: 1248 342
BanII G_rGCy'C
Cuts at: 0 555 1453 1590
Size: 555 898 137
Fragments arranged by size:
898 555 137
BbeI G_GCGC'C
Cuts at: 0 1252 1590
Size: 1252 338
BbsI GAAGACnn'nnnn_
Cuts at: 0 100 665 1590
Size: 100 565 925
Fragments arranged by size:
925 565 100
BbvCI CC'TCA_GC
Cuts at: 0 1018 1590
Size: 1018 572
BciVI GTATCCnnnnn_n'
Cuts at: 0 1282 1590
Size: 1282 308
BglI GCCn_nnn'nGGC
Cuts at: 0 1111 1144 1590
Size: 1111 33 446
Fragments arranged by size:
1111 446 33
Bme1580I G_kGCm'C
Cuts at: 0 26 395 1166 1190 1453 1511 1590
Size: 26 369 771 24 263 58 79
Fragments arranged by size:
771 369 263 79 58 26 24
BmgBI CAC'GTC
Cuts at: 0 15 578 618 1590
Size: 15 563 40 972
Fragments arranged by size:
972 563 40 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 1125 1135 1590
Size: 1125 10 455
Fragments arranged by size:
1125 455 10
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 322 1590
Size: 322 1268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 333 976 1473 1590
Size: 333 643 497 117
Fragments arranged by size:
643 497 333 117
Bpu10I CC'TnA_GC
Cuts at: 0 457 565 1018 1369 1590
Size: 457 108 453 351 221
Fragments arranged by size:
457 453 351 221 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1590
Size: 196 1394
BsaI GGTCTCn'nnnn_
Cuts at: 0 632 643 769 1053 1590
Size: 632 11 126 284 537
Fragments arranged by size:
632 537 284 126 11
BsaAI yAC'GTr
Cuts at: 0 1087 1590
Size: 1087 503
BsaBI GATnn'nnATC
Cuts at: 0 974 1590
Size: 974 616
BsaHI Gr'CG_yC
Cuts at: 0 725 1042 1249 1590
Size: 725 317 207 341
Fragments arranged by size:
725 341 317 207
BsaWI w'CCGG_w
Cuts at: 0 54 1590
Size: 54 1536
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 542 572 949 979 1590
Size: 542 30 377 30 611
Fragments arranged by size:
611 542 377 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 192 1549 1590
Size: 46 146 1357 41
Fragments arranged by size:
1357 146 46 41
BseYI C'CCAG_C
Cuts at: 0 206 1010 1172 1324 1590
Size: 206 804 162 152 266
Fragments arranged by size:
804 266 206 162 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1405 1590
Size: 874 531 185
Fragments arranged by size:
874 531 185
BsiEI CG_ry'CG
Cuts at: 0 584 1106 1379 1590
Size: 584 522 273 211
Fragments arranged by size:
584 522 273 211
BsiHKAI G_wGCw'C
Cuts at: 0 26 395 1031 1127 1375 1541 1590
Size: 26 369 636 96 248 166 49
Fragments arranged by size:
636 369 248 166 96 49 26
Bsp1286I G_dGCh'C
Cuts at: 0 26 395 555 1031 1127 1166 1190 1375
Size: 26 369 160 476 96 39 24 185
Cuts at: 1375 1453 1511 1541 1590
Size: 78 58 30 49
Fragments arranged by size:
476 369 185 160 96 78 58 49
39 30 26 24
BspEI T'CCGG_A
Cuts at: 0 54 1590
Size: 54 1536
BspHI T'CATG_A
Cuts at: 0 1031 1590
Size: 1031 559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 529 1110 1590
Size: 529 581 480
Fragments arranged by size:
581 529 480
BsrBI CCG'CTC
Cuts at: 0 920 1584 1590
Size: 920 664 6
Fragments arranged by size:
920 664 6
BsrFI r'CCGG_y
Cuts at: 0 727 1044 1101 1590
Size: 727 317 57 489
Fragments arranged by size:
727 489 317 57
BssSI C'ACGA_G
Cuts at: 0 1579 1590
Size: 1579 11
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1519 1590
Size: 1519 71
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1227 1332 1557 1590
Size: 1227 105 225 33
Fragments arranged by size:
1227 225 105 33
BstYI r'GATC_y
Cuts at: 0 1337 1590
Size: 1337 253
Bsu36I CC'TnA_GG
Cuts at: 0 1532 1590
Size: 1532 58
BtgI C'CryG_G
Cuts at: 0 185 677 1109 1565 1590
Size: 185 492 432 456 25
Fragments arranged by size:
492 456 432 185 25
BtsI GCAGTG_nn'
Cuts at: 0 59 71 81 970 1590
Size: 59 12 10 889 620
Fragments arranged by size:
889 620 59 12 10
EaeI y'GGCC_r
Cuts at: 0 398 581 680 893 1103 1376 1544 1590
Size: 398 183 99 213 210 273 168 46
Fragments arranged by size:
398 273 213 210 183 168 99 46
EagI C'GGCC_G
Cuts at: 0 581 1103 1376 1590
Size: 581 522 273 214
Fragments arranged by size:
581 522 273 214
EarI CTCTTCn'nnn_
Cuts at: 0 236 421 1311 1590
Size: 236 185 890 279
Fragments arranged by size:
890 279 236 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 333 436 739 976 1018 1228 1473
Size: 260 73 103 303 237 42 210 245
Cuts at: 1473 1590
Size: 117
Fragments arranged by size:
303 260 245 237 210 117 103 73
42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1231 1590
Size: 1231 359
EcoO109I rG'GnC_Cy
Cuts at: 0 807 942 1450 1590
Size: 807 135 508 140
Fragments arranged by size:
807 508 140 135
HaeII r_GCGC'y
Cuts at: 0 1150 1252 1590
Size: 1150 102 338
Fragments arranged by size:
1150 338 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1280 1312 1590
Size: 1280 32 278
Fragments arranged by size:
1280 278 32
KasI G'GCGC_C
Cuts at: 0 1248 1590
Size: 1248 342
MfeI C'AATT_G
Cuts at: 0 1496 1590
Size: 1496 94
MluI A'CGCG_T
Cuts at: 0 1349 1590
Size: 1349 241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 1590
Size: 539 1051
MscI TGG'CCA
Cuts at: 0 895 1590
Size: 895 695
MslI CAynn'nnrTG
Cuts at: 0 64 340 439 1498 1555 1590
Size: 64 276 99 1059 57 35
Fragments arranged by size:
1059 276 99 64 57 35
MspA1I CmG'CkG
Cuts at: 0 172 181 187 679 798 1176 1278 1422
Size: 172 9 6 492 119 378 102 144
Cuts at: 1422 1428 1590
Size: 6 162
Fragments arranged by size:
492 378 172 162 144 119 102 9
6 6
NaeI GCC'GGC
Cuts at: 0 729 1590
Size: 729 861
NarI GG'CG_CC
Cuts at: 0 1249 1590
Size: 1249 341
NcoI C'CATG_G
Cuts at: 0 1109 1565 1590
Size: 1109 456 25
Fragments arranged by size:
1109 456 25
NgoMIV G'CCGG_C
Cuts at: 0 727 1590
Size: 727 863
NspI r_CATG'y
Cuts at: 0 370 870 1590
Size: 370 500 720
Fragments arranged by size:
720 500 370
PflMI CCAn_nnn'nTGG
Cuts at: 0 768 1590
Size: 768 822
PfoI T'CCnGG_A
Cuts at: 0 749 803 1590
Size: 749 54 787
Fragments arranged by size:
787 749 54
PmlI CAC'GTG
Cuts at: 0 1087 1590
Size: 1087 503
PpuMI rG'GwC_Cy
Cuts at: 0 807 942 1590
Size: 807 135 648
Fragments arranged by size:
807 648 135
PshAI GACnn'nnGTC
Cuts at: 0 1045 1590
Size: 1045 545
PspOMI G'GGCC_C
Cuts at: 0 1449 1590
Size: 1449 141
PstI C_TGCA'G
Cuts at: 0 66 794 821 1427 1463 1590
Size: 66 728 27 606 36 127
Fragments arranged by size:
728 606 127 66 36 27
PvuII CAG'CTG
Cuts at: 0 181 1176 1428 1590
Size: 181 995 252 162
Fragments arranged by size:
995 252 181 162
SacII CC_GC'GG
Cuts at: 0 188 680 1590
Size: 188 492 910
Fragments arranged by size:
910 492 188
SexAI A'CCwGG_T
Cuts at: 0 875 1590
Size: 875 715
SfcI C'TryA_G
Cuts at: 0 62 790 817 1423 1459 1590
Size: 62 728 27 606 36 131
Fragments arranged by size:
728 606 131 62 36 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1111 1590
Size: 1111 479
SfoI GGC'GCC
Cuts at: 0 1250 1590
Size: 1250 340
SmaI CCC'GGG
Cuts at: 0 940 1157 1527 1590
Size: 940 217 370 63
Fragments arranged by size:
940 370 217 63
SmlI C'TyrA_G
Cuts at: 0 211 1590
Size: 211 1379
SphI G_CATG'C
Cuts at: 0 870 1590
Size: 870 720
SrfI GCCC'GGGC
Cuts at: 0 1157 1590
Size: 1157 433
StuI AGG'CCT
Cuts at: 0 788 1477 1590
Size: 788 689 113
Fragments arranged by size:
788 689 113
StyI C'CwwG_G
Cuts at: 0 989 1109 1191 1565 1590
Size: 989 120 82 374 25
Fragments arranged by size:
989 374 120 82 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1144 1590
Size: 1144 446
TatI w'GTAC_w
Cuts at: 0 587 834 1590
Size: 587 247 756
Fragments arranged by size:
756 587 247
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 542 574 949 981 1590
Size: 542 32 375 32 609
Fragments arranged by size:
609 542 375 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 505 742 1590
Size: 505 237 848
Fragments arranged by size:
848 505 237
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1480 1554 1590
Size: 1480 74 36
Fragments arranged by size:
1480 74 36
XmaI C'CCGG_G
Cuts at: 0 938 1155 1525 1590
Size: 938 217 370 65
Fragments arranged by size:
938 370 217 65
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BciVI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI
EagI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PflMI PfoI PmlI PpuMI PshAI
PspOMI PstI PvuII SacII SexAI SfcI SfiI SfoI
SmaI SmlI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BclI BfrBI BglII BlpI BmtI BsiWI BsmI
BsmBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI NdeI NheI NotI NruI NsiI PacI PasI
PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SgfI SgrAI
SnaBI SpeI SspI SwaI XbaI XhoI XmnI ZraI