Restriction map for
pF1KA1270
(Linear) (Six-base) MAPSORT of: KIAA1270.seq Check: 1197 from: 1 to: 2955
KIAA1270.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1916 2955
Size: 1916 1039
AccI GT'mk_AC
Cuts at: 0 1615 2955
Size: 1615 1340
Acc65I G'GTAC_C
Cuts at: 0 1125 2955
Size: 1125 1830
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1268 1322 1700 2955
Size: 1268 54 378 1255
Fragments arranged by size:
1268 1255 378 54
AfeI AGC'GCT
Cuts at: 0 1660 2955
Size: 1660 1295
AflIII A'CryG_T
Cuts at: 0 300 2955
Size: 300 2655
AleI CACnn'nnGTG
Cuts at: 0 2731 2955
Size: 2731 224
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2576 2749 2955
Size: 2576 173 206
Fragments arranged by size:
2576 206 173
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1744 1776 2955
Size: 1744 32 1179
Fragments arranged by size:
1744 1179 32
AlwNI CAG_nnn'CTG
Cuts at: 0 774 998 1935 2158 2385 2619 2665 2955
Size: 774 224 937 223 227 234 46 290
Fragments arranged by size:
937 774 290 234 227 224 223 46
ApaI G_GGCC'C
Cuts at: 0 659 1779 1942 2955
Size: 659 1120 163 1013
Fragments arranged by size:
1120 1013 659 163
AvaI C'yCGr_G
Cuts at: 0 1772 2366 2955
Size: 1772 594 589
Fragments arranged by size:
1772 594 589
AvrII C'CTAG_G
Cuts at: 0 1113 2955
Size: 1113 1842
BamHI G'GATC_C
Cuts at: 0 270 482 1055 2955
Size: 270 212 573 1900
Fragments arranged by size:
1900 573 270 212
BanI G'GyrC_C
Cuts at: 0 174 262 582 903 1098 1125 1585 2094
Size: 174 88 320 321 195 27 460 509
Cuts at: 2094 2181 2794 2887 2955
Size: 87 613 93 68
Fragments arranged by size:
613 509 460 321 320 195 174 93
88 87 68 27
BanII G_rGCy'C
Cuts at: 0 101 659 702 1779 1942 1963 2869 2955
Size: 101 558 43 1077 163 21 906 86
Fragments arranged by size:
1077 906 558 163 101 86 43 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 723 1008 2955
Size: 723 285 1947
Fragments arranged by size:
1947 723 285
BbvCI CC'TCA_GC
Cuts at: 0 73 2944 2955
Size: 73 2871 11
Fragments arranged by size:
2871 73 11
BciVI GTATCCnnnnn_n'
Cuts at: 0 633 2955
Size: 633 2322
BfrBI ATG'CAT
Cuts at: 0 2197 2955
Size: 2197 758
BglI GCCn_nnn'nGGC
Cuts at: 0 63 127 783 1225 2362 2514 2955
Size: 63 64 656 442 1137 152 441
Fragments arranged by size:
1137 656 442 441 152 64 63
BglII A'GATC_T
Cuts at: 0 1088 2955
Size: 1088 1867
BlpI GC'TnA_GC
Cuts at: 0 571 1451 2955
Size: 571 880 1504
Fragments arranged by size:
1504 880 571
Bme1580I G_kGCm'C
Cuts at: 0 179 265 659 906 1443 1523 1779 1923
Size: 179 86 394 247 537 80 256 144
Cuts at: 1923 1942 2099 2186 2323 2829 2955
Size: 19 157 87 137 506 126
Fragments arranged by size:
537 506 394 256 247 179 157 144
137 126 87 86 80 19
BmgBI CAC'GTC
Cuts at: 0 301 1755 2729 2955
Size: 301 1454 974 226
Fragments arranged by size:
1454 974 301 226
BmrI ACTGGGnnnn_n'
Cuts at: 0 203 1156 1759 1926 2279 2354 2505 2955
Size: 203 953 603 167 353 75 151 450
Fragments arranged by size:
953 603 450 353 203 167 151 75
BmtI G_CTAG'C
Cuts at: 0 593 2955
Size: 593 2362
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2649 2694 2955
Size: 2649 45 261
Fragments arranged by size:
2649 261 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 571 1255 1390 1426 2644 2955
Size: 353 218 684 135 36 1218 311
Fragments arranged by size:
1218 684 353 311 218 135 36
Bpu10I CC'TnA_GC
Cuts at: 0 73 576 1976 2944 2955
Size: 73 503 1400 968 11
Fragments arranged by size:
1400 968 503 73 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 362 547 1325 2955
Size: 362 185 778 1630
Fragments arranged by size:
1630 778 362 185
BsaAI yAC'GTr
Cuts at: 0 2266 2955
Size: 2266 689
BsaBI GATnn'nnATC
Cuts at: 0 1157 2955
Size: 1157 1798
BsaHI Gr'CG_yC
Cuts at: 0 1013 1872 2955
Size: 1013 859 1083
Fragments arranged by size:
1083 1013 859
BsaWI w'CCGG_w
Cuts at: 0 2890 2955
Size: 2890 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1537 1567 2194 2224 2955
Size: 1537 30 627 30 731
Fragments arranged by size:
1537 731 627 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 451 1387 1438 1660 2104 2955
Size: 451 936 51 222 444 851
Fragments arranged by size:
936 851 451 444 222 51
BseYI C'CCAG_C
Cuts at: 0 704 779 1430 1564 2205 2357 2722 2750
Size: 704 75 651 134 641 152 365 28
Cuts at: 2750 2955
Size: 205
Fragments arranged by size:
704 651 641 365 205 152 134 75
28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1867 2071 2859 2955
Size: 1867 204 788 96
Fragments arranged by size:
1867 788 204 96
BsiEI CG_ry'CG
Cuts at: 0 85 122 2955
Size: 85 37 2833
Fragments arranged by size:
2833 85 37
BsiHKAI G_wGCw'C
Cuts at: 0 2738 2955
Size: 2738 217
Bsp1286I G_dGCh'C
Cuts at: 0 101 179 265 659 702 906 1443 1523
Size: 101 78 86 394 43 204 537 80
Cuts at: 1523 1779 1923 1942 1963 2099 2186 2323 2738
Size: 256 144 19 21 136 87 137 415
Cuts at: 2738 2829 2869 2955
Size: 91 40 86
Fragments arranged by size:
537 415 394 256 204 144 137 136
101 91 87 86 86 80 78 43
40 21 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1916 2955
Size: 1916 1039
BsrBI CCG'CTC
Cuts at: 0 87 1239 1410 2415 2628 2955
Size: 87 1152 171 1005 213 327
Fragments arranged by size:
1152 1005 327 213 171 87
BsrFI r'CCGG_y
Cuts at: 0 167 2955
Size: 167 2788
BsrGI T'GTAC_A
Cuts at: 0 2081 2955
Size: 2081 874
BstAPI GCAn_nnn'nTGC
Cuts at: 0 774 2955
Size: 774 2181
BstBI TT'CG_AA
Cuts at: 0 48 2955
Size: 48 2907
BstEII G'GTnAC_C
Cuts at: 0 520 2955
Size: 520 2435
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1495 1663 1786 2122 2955
Size: 1495 168 123 336 833
Fragments arranged by size:
1495 833 336 168 123
BstYI r'GATC_y
Cuts at: 0 270 482 503 668 1055 1088 2955
Size: 270 212 21 165 387 33 1867
Fragments arranged by size:
1867 387 270 212 165 33 21
BstZ17I GTA'TAC
Cuts at: 0 1616 2955
Size: 1616 1339
Bsu36I CC'TnA_GG
Cuts at: 0 488 2955
Size: 488 2467
BtgI C'CryG_G
Cuts at: 0 159 198 266 1082 1643 1722 2060 2186
Size: 159 39 68 816 561 79 338 126
Cuts at: 2186 2756 2955
Size: 570 199
Fragments arranged by size:
816 570 561 338 199 159 126 79
68 39
BtsI GCAGTG_nn'
Cuts at: 0 1640 2042 2108 2221 2567 2840 2955
Size: 1640 402 66 113 346 273 115
Fragments arranged by size:
1640 402 346 273 115 113 66
DraIII CAC_nnn'GTG
Cuts at: 0 994 1327 2955
Size: 994 333 1628
Fragments arranged by size:
1628 994 333
EaeI y'GGCC_r
Cuts at: 0 82 119 162 305 1898 2336 2405 2534
Size: 82 37 43 143 1593 438 69 129
Cuts at: 2534 2955
Size: 421
Fragments arranged by size:
1593 438 421 143 129 82 69 43
37
EagI C'GGCC_G
Cuts at: 0 82 119 2955
Size: 82 37 2836
Fragments arranged by size:
2836 82 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1263 2458 2955
Size: 1263 1195 497
Fragments arranged by size:
1263 1195 497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 571 1255 1268 1322 1390 1426 1700
Size: 353 218 684 13 54 68 36 274
Cuts at: 1700 2644 2955
Size: 944 311
Fragments arranged by size:
944 684 353 311 274 218 68 54
36 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1804 2389 2955
Size: 1804 585 566
Fragments arranged by size:
1804 585 566
EcoO109I rG'GnC_Cy
Cuts at: 0 69 656 1033 1271 1279 1939 2118 2283
Size: 69 587 377 238 8 660 179 165
Cuts at: 2283 2641 2718 2955
Size: 358 77 237
Fragments arranged by size:
660 587 377 358 238 237 179 165
77 69 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 270 2955
Size: 270 2685
HaeII r_GCGC'y
Cuts at: 0 1512 1662 2107 2955
Size: 1512 150 445 848
Fragments arranged by size:
1512 848 445 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1017 1047 1049 1079 1242 1274 1781 1813
Size: 1017 30 2 30 163 32 507 32
Cuts at: 1813 2194 2226 2647 2679 2955
Size: 381 32 421 32 276
Fragments arranged by size:
1017 507 421 381 276 163 32 32
32 32 30 30 2
KpnI G_GTAC'C
Cuts at: 0 1129 2955
Size: 1129 1826
MfeI C'AATT_G
Cuts at: 0 405 2955
Size: 405 2550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 1033 2955
Size: 321 712 1922
Fragments arranged by size:
1922 712 321
MscI TGG'CCA
Cuts at: 0 164 307 2536 2955
Size: 164 143 2229 419
Fragments arranged by size:
2229 419 164 143
MslI CAynn'nnrTG
Cuts at: 0 799 1859 2731 2955
Size: 799 1060 872 224
Fragments arranged by size:
1060 872 799 224
MspA1I CmG'CkG
Cuts at: 0 19 200 708 783 873 1041 1399 1568
Size: 19 181 508 75 90 168 358 169
Cuts at: 1568 1851 1983 2520 2556 2706 2955
Size: 283 132 537 36 150 249
Fragments arranged by size:
537 508 358 283 249 181 169 168
150 132 90 75 36 19
NcoI C'CATG_G
Cuts at: 0 159 1082 2756 2955
Size: 159 923 1674 199
Fragments arranged by size:
1674 923 199 159
NheI G'CTAG_C
Cuts at: 0 589 2955
Size: 589 2366
NruI TCG'CGA
Cuts at: 0 2871 2955
Size: 2871 84
NsiI A_TGCA'T
Cuts at: 0 2199 2955
Size: 2199 756
NspI r_CATG'y
Cuts at: 0 790 1858 2955
Size: 790 1068 1097
Fragments arranged by size:
1097 1068 790
PasI CC'CwG_GG
Cuts at: 0 1021 1275 1935 2790 2955
Size: 1021 254 660 855 165
Fragments arranged by size:
1021 855 660 254 165
PflMI CCAn_nnn'nTGG
Cuts at: 0 171 2199 2385 2757 2955
Size: 171 2028 186 372 198
Fragments arranged by size:
2028 372 198 186 171
PpuMI rG'GwC_Cy
Cuts at: 0 1033 1271 2118 2283 2955
Size: 1033 238 847 165 672
Fragments arranged by size:
1033 847 672 238 165
PshAI GACnn'nnGTC
Cuts at: 0 472 2955
Size: 472 2483
PspOMI G'GGCC_C
Cuts at: 0 655 1775 1938 2955
Size: 655 1120 163 1017
Fragments arranged by size:
1120 1017 655 163
PstI C_TGCA'G
Cuts at: 0 24 108 770 901 1508 2228 2555 2955
Size: 24 84 662 131 607 720 327 400
Fragments arranged by size:
720 662 607 400 327 131 84 24
PvuII CAG'CTG
Cuts at: 0 19 708 1851 1983 2706 2955
Size: 19 689 1143 132 723 249
Fragments arranged by size:
1143 723 689 249 132 19
SacII CC_GC'GG
Cuts at: 0 201 2955
Size: 201 2754
SexAI A'CCwGG_T
Cuts at: 0 725 1196 1730 1841 2285 2955
Size: 725 471 534 111 444 670
Fragments arranged by size:
725 670 534 471 444 111
SfcI C'TryA_G
Cuts at: 0 20 104 766 897 1130 1504 2224 2276
Size: 20 84 662 131 233 374 720 52
Cuts at: 2276 2551 2955
Size: 275 404
Fragments arranged by size:
720 662 404 374 275 233 131 84
52 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 127 2362 2955
Size: 127 2235 593
Fragments arranged by size:
2235 593 127
SmaI CCC'GGG
Cuts at: 0 1774 2955
Size: 1774 1181
SnaBI TAC'GTA
Cuts at: 0 2266 2955
Size: 2266 689
SrfI GCCC'GGGC
Cuts at: 0 1774 2955
Size: 1774 1181
SspI AAT'ATT
Cuts at: 0 427 2955
Size: 427 2528
StuI AGG'CCT
Cuts at: 0 115 1867 2821 2955
Size: 115 1752 954 134
Fragments arranged by size:
1752 954 134 115
StyI C'CwwG_G
Cuts at: 0 110 159 527 1082 1113 1346 1512 2315
Size: 110 49 368 555 31 233 166 803
Cuts at: 2315 2462 2756 2883 2955
Size: 147 294 127 72
Fragments arranged by size:
803 555 368 294 233 166 147 127
110 72 49 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1918 2017 2955
Size: 1918 99 938
Fragments arranged by size:
1918 938 99
TatI w'GTAC_w
Cuts at: 0 661 1544 2081 2955
Size: 661 883 537 874
Fragments arranged by size:
883 874 661 537
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 255 287 1094 1126 2955
Size: 255 32 807 32 1829
Fragments arranged by size:
1829 807 255 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2659 2955
Size: 2659 296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 534 2955
Size: 534 2421
XmaI C'CCGG_G
Cuts at: 0 1772 2955
Size: 1772 1183
XmnI GAAnn'nnTTC
Cuts at: 0 241 2955
Size: 241 2714
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlfI
AloI AlwNI ApaI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BciVI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII
EaeI EagI EciI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I KpnI MfeI MmeI MscI MslI MspA1I NcoI
NheI NruI NsiI NspI PasI PflMI PpuMI PshAI
PspOMI PstI PvuII SacII SexAI SfcI SfiI SmaI
SnaBI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI ApaLI ApoI AscI
AseI AsiSI BaeI BbeI BcgI BclI BpuEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BssHII BssSI BtgZI
ClaI CspCI DraI DrdI EarI EcoICRI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI MluI
NaeI NarI NdeI NgoMIV NotI PacI PciI PfoI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SfoI SgfI
SgrAI SmlI SpeI SphI SwaI XbaI XhoI ZraI