Restriction map for
pF1KA1273
(Linear) (Six-base) MAPSORT of: KIAA1273.seq Check: 9681 from: 1 to: 1806
KIAA1273.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AatII G_ACGT'C
Cuts at: 0 727 1806
Size: 727 1079
AccI GT'mk_AC
Cuts at: 0 644 789 1806
Size: 644 145 1017
Fragments arranged by size:
1017 644 145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 520 1393 1597 1806
Size: 316 204 873 204 209
Fragments arranged by size:
873 316 209 204 204
AfeI AGC'GCT
Cuts at: 0 280 1806
Size: 280 1526
AflII C'TTAA_G
Cuts at: 0 1342 1806
Size: 1342 464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 754 1806
Size: 754 1052
ApaI G_GGCC'C
Cuts at: 0 252 1806
Size: 252 1554
ApoI r'AATT_y
Cuts at: 0 324 1198 1806
Size: 324 874 608
Fragments arranged by size:
874 608 324
AvaI C'yCGr_G
Cuts at: 0 440 1806
Size: 440 1366
BanI G'GyrC_C
Cuts at: 0 550 755 925 1367 1793 1806
Size: 550 205 170 442 426 13
Fragments arranged by size:
550 442 426 205 170 13
BanII G_rGCy'C
Cuts at: 0 252 1806
Size: 252 1554
BbeI G_GCGC'C
Cuts at: 0 1371 1806
Size: 1371 435
BbsI GAAGACnn'nnnn_
Cuts at: 0 217 940 1806
Size: 217 723 866
Fragments arranged by size:
866 723 217
BbvCI CC'TCA_GC
Cuts at: 0 197 1806
Size: 197 1609
BglI GCCn_nnn'nGGC
Cuts at: 0 613 1073 1685 1806
Size: 613 460 612 121
Fragments arranged by size:
613 612 460 121
BlpI GC'TnA_GC
Cuts at: 0 1454 1806
Size: 1454 352
Bme1580I G_kGCm'C
Cuts at: 0 45 252 1735 1796 1806
Size: 45 207 1483 61 10
Fragments arranged by size:
1483 207 61 45 10
BmgBI CAC'GTC
Cuts at: 0 801 1002 1266 1806
Size: 801 201 264 540
Fragments arranged by size:
801 540 264 201
BmrI ACTGGGnnnn_n'
Cuts at: 0 246 1062 1377 1733 1806
Size: 246 816 315 356 73
Fragments arranged by size:
816 356 315 246 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 826 1806
Size: 763 63 980
Fragments arranged by size:
980 763 63
Bpu10I CC'TnA_GC
Cuts at: 0 107 197 217 1229 1806
Size: 107 90 20 1012 577
Fragments arranged by size:
1012 577 107 90 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1806
Size: 154 1652
BsaI GGTCTCn'nnnn_
Cuts at: 0 220 424 1627 1806
Size: 220 204 1203 179
Fragments arranged by size:
1203 220 204 179
BsaHI Gr'CG_yC
Cuts at: 0 505 724 1103 1368 1526 1806
Size: 505 219 379 265 158 280
Fragments arranged by size:
505 379 280 265 219 158
BsaWI w'CCGG_w
Cuts at: 0 1618 1806
Size: 1618 188
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 73 103 1420 1450 1806
Size: 73 30 1317 30 356
Fragments arranged by size:
1317 356 73 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 219 238 334 355 772 1144 1312
Size: 214 5 19 96 21 417 372 168
Cuts at: 1312 1806
Size: 494
Fragments arranged by size:
494 417 372 214 168 96 21 19
5
BseYI C'CCAG_C
Cuts at: 0 616 696 826 1806
Size: 616 80 130 980
Fragments arranged by size:
980 616 130 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 91 370 946 1677 1806
Size: 10 81 279 576 731 129
Fragments arranged by size:
731 576 279 129 81 10
BsiEI CG_ry'CG
Cuts at: 0 1773 1777 1806
Size: 1773 4 29
Fragments arranged by size:
1773 29 4
BsiHKAI G_wGCw'C
Cuts at: 0 138 515 1408 1610 1806
Size: 138 377 893 202 196
Fragments arranged by size:
893 377 202 196 138
BsmI GAATG_Cn'
Cuts at: 0 474 663 1806
Size: 474 189 1143
Fragments arranged by size:
1143 474 189
BsmBI CGTCTCn'nnnn_
Cuts at: 0 715 1806
Size: 715 1091
Bsp1286I G_dGCh'C
Cuts at: 0 45 138 252 515 1408 1610 1735 1796
Size: 45 93 114 263 893 202 125 61
Cuts at: 1796 1806
Size: 10
Fragments arranged by size:
893 263 202 125 114 93 61 45
10
BspEI T'CCGG_A
Cuts at: 0 1618 1806
Size: 1618 188
BspHI T'CATG_A
Cuts at: 0 986 1806
Size: 986 820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 29 1292 1806
Size: 29 1263 514
Fragments arranged by size:
1263 514 29
BsrBI CCG'CTC
Cuts at: 0 84 387 462 1302 1806
Size: 84 303 75 840 504
Fragments arranged by size:
840 504 303 84 75
BsrFI r'CCGG_y
Cuts at: 0 773 1067 1347 1684 1806
Size: 773 294 280 337 122
Fragments arranged by size:
773 337 294 280 122
BsrGI T'GTAC_A
Cuts at: 0 893 1806
Size: 893 913
BssHII G'CGCG_C
Cuts at: 0 445 447 1806
Size: 445 2 1359
Fragments arranged by size:
1359 445 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 906 1806
Size: 906 900
BstYI r'GATC_y
Cuts at: 0 182 491 1806
Size: 182 309 1315
Fragments arranged by size:
1315 309 182
BtgI C'CryG_G
Cuts at: 0 158 380 608 1010 1478 1658 1806
Size: 158 222 228 402 468 180 148
Fragments arranged by size:
468 402 228 222 180 158 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 837 1806
Size: 837 969
BtsI GCAGTG_nn'
Cuts at: 0 102 1806
Size: 102 1704
DrdI GACnn_nn'nnGTC
Cuts at: 0 1270 1806
Size: 1270 536
EaeI y'GGCC_r
Cuts at: 0 675 1214 1349 1460 1655 1774 1806
Size: 675 539 135 111 195 119 32
Fragments arranged by size:
675 539 195 135 119 111 32
EagI C'GGCC_G
Cuts at: 0 1774 1806
Size: 1774 32
EarI CTCTTCn'nnn_
Cuts at: 0 1090 1806
Size: 1090 716
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 484 1597 1806
Size: 484 1113 209
Fragments arranged by size:
1113 484 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 520 763 826 1393 1597 1806
Size: 316 204 243 63 567 204 209
Fragments arranged by size:
567 316 243 209 204 204 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 220 1510 1806
Size: 220 1290 296
Fragments arranged by size:
1290 296 220
EcoO109I rG'GnC_Cy
Cuts at: 0 248 888 1143 1503 1588 1738 1806
Size: 248 640 255 360 85 150 68
Fragments arranged by size:
640 360 255 248 150 85 68
HaeII r_GCGC'y
Cuts at: 0 282 751 1311 1371 1806
Size: 282 469 560 60 435
Fragments arranged by size:
560 469 435 282 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 73 105 1420 1452 1806
Size: 73 32 1315 32 354
Fragments arranged by size:
1315 354 73 32 32
HincII GTy'rAC
Cuts at: 0 790 1806
Size: 790 1016
KasI G'GCGC_C
Cuts at: 0 1367 1806
Size: 1367 439
MscI TGG'CCA
Cuts at: 0 1216 1657 1806
Size: 1216 441 149
Fragments arranged by size:
1216 441 149
MslI CAynn'nnrTG
Cuts at: 0 410 736 1264 1806
Size: 410 326 528 542
Fragments arranged by size:
542 528 410 326
MspA1I CmG'CkG
Cuts at: 0 6 121 1458 1806
Size: 6 115 1337 348
Fragments arranged by size:
1337 348 115 6
NaeI GCC'GGC
Cuts at: 0 775 1069 1349 1686 1806
Size: 775 294 280 337 120
Fragments arranged by size:
775 337 294 280 120
NarI GG'CG_CC
Cuts at: 0 1368 1806
Size: 1368 438
NcoI C'CATG_G
Cuts at: 0 1010 1806
Size: 1010 796
NdeI CA'TA_TG
Cuts at: 0 1485 1806
Size: 1485 321
NgoMIV G'CCGG_C
Cuts at: 0 773 1067 1347 1684 1806
Size: 773 294 280 337 122
Fragments arranged by size:
773 337 294 280 122
NspI r_CATG'y
Cuts at: 0 1437 1806
Size: 1437 369
PasI CC'CwG_GG
Cuts at: 0 1735 1789 1806
Size: 1735 54 17
Fragments arranged by size:
1735 54 17
PfoI T'CCnGG_A
Cuts at: 0 1711 1806
Size: 1711 95
PpuMI rG'GwC_Cy
Cuts at: 0 1143 1503 1806
Size: 1143 360 303
Fragments arranged by size:
1143 360 303
PshAI GACnn'nnGTC
Cuts at: 0 667 1806
Size: 667 1139
PspOMI G'GGCC_C
Cuts at: 0 248 1806
Size: 248 1558
PstI C_TGCA'G
Cuts at: 0 143 1806
Size: 143 1663
PvuII CAG'CTG
Cuts at: 0 6 121 1458 1806
Size: 6 115 1337 348
Fragments arranged by size:
1337 348 115 6
SalI G'TCGA_C
Cuts at: 0 788 1806
Size: 788 1018
SapI GCTCTTCn'nnn_
Cuts at: 0 1090 1806
Size: 1090 716
SfcI C'TryA_G
Cuts at: 0 139 1806
Size: 139 1667
SfoI GGC'GCC
Cuts at: 0 1369 1806
Size: 1369 437
SmaI CCC'GGG
Cuts at: 0 442 1806
Size: 442 1364
SmlI C'TyrA_G
Cuts at: 0 169 1342 1806
Size: 169 1173 464
Fragments arranged by size:
1173 464 169
SpeI A'CTAG_T
Cuts at: 0 33 1806
Size: 33 1773
SrfI GCCC'GGGC
Cuts at: 0 442 1806
Size: 442 1364
StyI C'CwwG_G
Cuts at: 0 452 1010 1493 1626 1806
Size: 452 558 483 133 180
Fragments arranged by size:
558 483 452 180 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 448 1806
Size: 448 1358
TatI w'GTAC_w
Cuts at: 0 893 1806
Size: 893 913
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1600 1632 1746 1778 1806
Size: 1600 32 114 32 28
Fragments arranged by size:
1600 114 32 32 28
Tth111I GACn'n_nGTC
Cuts at: 0 1501 1806
Size: 1501 305
XmaI C'CCGG_G
Cuts at: 0 440 1806
Size: 440 1366
XmnI GAAnn'nnTTC
Cuts at: 0 1165 1806
Size: 1165 641
ZraI GAC'GTC
Cuts at: 0 725 1806
Size: 725 1081
Enzymes that do cut:
AatII AccI AcuI AfeI AflII AlfI ApaI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BstAPI BstYI BtgI BtgZI BtsI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII KasI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PfoI PpuMI PshAI PspOMI
PstI PvuII SalI SapI SfcI SfoI SmaI SmlI
SpeI SrfI StyI TaqII TatI TstI Tth111I XmaI
XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflIII AgeI AhdI AleI AloI
AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BclI BfrBI BglII BmtI BplI BsaAI
BsaBI BsiWI BsrDI BssSI BstBI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraI DraIII EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MmeI NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
RsrII SacI SacII SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SphI SspI StuI SwaI XbaI
XcmI XhoI