Restriction map for
pF1KA1275
(Linear) (Six-base) MAPSORT of: KIAA1275.seq Check: 6613 from: 1 to: 3531
KIAA1275.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1873 2508 3531
Size: 1873 635 1023
Fragments arranged by size:
1873 1023 635
AatII G_ACGT'C
Cuts at: 0 2404 3531
Size: 2404 1127
AccI GT'mk_AC
Cuts at: 0 539 1694 3037 3531
Size: 539 1155 1343 494
Fragments arranged by size:
1343 1155 539 494
AclI AA'CG_TT
Cuts at: 0 2876 3531
Size: 2876 655
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 1936 2816 3227 3531
Size: 322 1614 880 411 304
Fragments arranged by size:
1614 880 411 322 304
AfeI AGC'GCT
Cuts at: 0 2341 3531
Size: 2341 1190
AgeI A'CCGG_T
Cuts at: 0 3396 3531
Size: 3396 135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 404 2078 3531
Size: 404 1674 1453
Fragments arranged by size:
1674 1453 404
AlwNI CAG_nnn'CTG
Cuts at: 0 160 822 2727 3531
Size: 160 662 1905 804
Fragments arranged by size:
1905 804 662 160
ApaI G_GGCC'C
Cuts at: 0 2663 3531
Size: 2663 868
ApaLI G'TGCA_C
Cuts at: 0 1344 3531
Size: 1344 2187
ApoI r'AATT_y
Cuts at: 0 1593 1996 2670 2800 3197 3531
Size: 1593 403 674 130 397 334
Fragments arranged by size:
1593 674 403 397 334 130
AseI AT'TA_AT
Cuts at: 0 2200 3531
Size: 2200 1331
AvaI C'yCGr_G
Cuts at: 0 3416 3531
Size: 3416 115
BanI G'GyrC_C
Cuts at: 0 2684 3531
Size: 2684 847
BanII G_rGCy'C
Cuts at: 0 1262 1862 2355 2588 2663 3531
Size: 1262 600 493 233 75 868
Fragments arranged by size:
1262 868 600 493 233 75
BbsI GAAGACnn'nnnn_
Cuts at: 0 239 310 878 1969 2974 3531
Size: 239 71 568 1091 1005 557
Fragments arranged by size:
1091 1005 568 557 239 71
BbvCI CC'TCA_GC
Cuts at: 0 823 3531
Size: 823 2708
BciVI GTATCCnnnnn_n'
Cuts at: 0 2576 3531
Size: 2576 955
BclI T'GATC_A
Cuts at: 0 1980 3531
Size: 1980 1551
BglII A'GATC_T
Cuts at: 0 4 2545 3531
Size: 4 2541 986
Fragments arranged by size:
2541 986 4
Bme1580I G_kGCm'C
Cuts at: 0 1348 2663 2687 3531
Size: 1348 1315 24 844
Fragments arranged by size:
1348 1315 844 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 1200 2357 2932 3420 3531
Size: 1200 1157 575 488 111
Fragments arranged by size:
1200 1157 575 488 111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 947 3531
Size: 947 2584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 238 352 631 1387 2215 2429 2555
Size: 206 32 114 279 756 828 214 126
Cuts at: 2555 2957 3128 3510 3531
Size: 402 171 382 21
Fragments arranged by size:
828 756 402 382 279 214 206 171
126 114 32 21
Bpu10I CC'TnA_GC
Cuts at: 0 823 3531
Size: 823 2708
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 874 917 982 3531
Size: 481 393 43 65 2549
Fragments arranged by size:
2549 481 393 65 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 3221 3531
Size: 3221 310
BsaHI Gr'CG_yC
Cuts at: 0 2401 3531
Size: 2401 1130
BsaWI w'CCGG_w
Cuts at: 0 3396 3531
Size: 3396 135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 410 440 2623 2653 2684 2714 3531
Size: 410 30 2183 30 31 30 817
Fragments arranged by size:
2183 817 410 31 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1042 1198 2059 3339 3531
Size: 1042 156 861 1280 192
Fragments arranged by size:
1280 1042 861 192 156
BseYI C'CCAG_C
Cuts at: 0 1349 3455 3531
Size: 1349 2106 76
Fragments arranged by size:
2106 1349 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 3531
Size: 364 3167
BsiHKAI G_wGCw'C
Cuts at: 0 1262 1348 1862 2355 2588 3382 3531
Size: 1262 86 514 493 233 794 149
Fragments arranged by size:
1262 794 514 493 233 149 86
BsmI GAATG_Cn'
Cuts at: 0 3476 3531
Size: 3476 55
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1930 3531
Size: 1930 1601
Bsp1286I G_dGCh'C
Cuts at: 0 1262 1348 1862 2355 2588 2663 2687 3382
Size: 1262 86 514 493 233 75 24 695
Cuts at: 3382 3531
Size: 149
Fragments arranged by size:
1262 695 514 493 233 149 86 75
24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 614 1403 1873 2508 3531
Size: 614 789 470 635 1023
Fragments arranged by size:
1023 789 635 614 470
BsrBI CCG'CTC
Cuts at: 0 621 3531
Size: 621 2910
BsrDI GCAATG_nn'
Cuts at: 0 81 3531
Size: 81 3450
BsrFI r'CCGG_y
Cuts at: 0 485 3313 3396 3531
Size: 485 2828 83 135
Fragments arranged by size:
2828 485 135 83
BssSI C'ACGA_G
Cuts at: 0 2752 3531
Size: 2752 779
BstAPI GCAn_nnn'nTGC
Cuts at: 0 507 3531
Size: 507 3024
BstBI TT'CG_AA
Cuts at: 0 1149 3531
Size: 1149 2382
BstXI CCAn_nnnn'nTGG
Cuts at: 0 609 2325 3531
Size: 609 1716 1206
Fragments arranged by size:
1716 1206 609
BstYI r'GATC_y
Cuts at: 0 4 807 2545 3531
Size: 4 803 1738 986
Fragments arranged by size:
1738 986 803 4
BstZ17I GTA'TAC
Cuts at: 0 540 1695 3531
Size: 540 1155 1836
Fragments arranged by size:
1836 1155 540
Bsu36I CC'TnA_GG
Cuts at: 0 153 323 2832 3531
Size: 153 170 2509 699
Fragments arranged by size:
2509 699 170 153
BtgI C'CryG_G
Cuts at: 0 2537 2611 3092 3531
Size: 2537 74 481 439
Fragments arranged by size:
2537 481 439 74
BtsI GCAGTG_nn'
Cuts at: 0 1779 2762 3531
Size: 1779 983 769
Fragments arranged by size:
1779 983 769
DraI TTT'AAA
Cuts at: 0 1361 1664 3531
Size: 1361 303 1867
Fragments arranged by size:
1867 1361 303
EaeI y'GGCC_r
Cuts at: 0 512 3531
Size: 512 3019
EarI CTCTTCn'nnn_
Cuts at: 0 109 830 938 1031 1282 1559 1786 2979
Size: 109 721 108 93 251 277 227 1193
Cuts at: 2979 3531
Size: 552
Fragments arranged by size:
1193 721 552 277 251 227 109 108
93
EcoICRI GAG'CTC
Cuts at: 0 1260 1860 2353 2586 3531
Size: 1260 600 493 233 945
Fragments arranged by size:
1260 945 600 493 233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 238 322 352 631 1387 1936 2215
Size: 206 32 84 30 279 756 549 279
Cuts at: 2215 2429 2555 2816 2957 3128 3227 3510 3531
Size: 214 126 261 141 171 99 283 21
Fragments arranged by size:
756 549 283 279 279 261 214 206
171 141 126 99 84 32 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2831 3531
Size: 2831 700
EcoO109I rG'GnC_Cy
Cuts at: 0 375 2659 2660 3531
Size: 375 2284 1 871
Fragments arranged by size:
2284 871 375 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1507 2040 2236 3531
Size: 1507 533 196 1295
Fragments arranged by size:
1507 1295 533 196
HaeII r_GCGC'y
Cuts at: 0 2343 3531
Size: 2343 1188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 410 442 2684 2716 3089 3121 3436 3468
Size: 410 32 2242 32 373 32 315 32
Cuts at: 3468 3531
Size: 63
Fragments arranged by size:
2242 410 373 315 63 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1779 2405 3531
Size: 1779 626 1126
Fragments arranged by size:
1779 1126 626
MfeI C'AATT_G
Cuts at: 0 1933 3531
Size: 1933 1598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 1953 2114 2306 3163 3300 3531
Size: 267 1686 161 192 857 137 231
Fragments arranged by size:
1686 857 267 231 192 161 137
MslI CAynn'nnrTG
Cuts at: 0 386 748 3481 3531
Size: 386 362 2733 50
Fragments arranged by size:
2733 386 362 50
MspA1I CmG'CkG
Cuts at: 0 504 1986 2432 2461 3055 3459 3531
Size: 504 1482 446 29 594 404 72
Fragments arranged by size:
1482 594 504 446 404 72 29
NcoI C'CATG_G
Cuts at: 0 2611 3092 3531
Size: 2611 481 439
Fragments arranged by size:
2611 481 439
NspI r_CATG'y
Cuts at: 0 300 495 3531
Size: 300 195 3036
Fragments arranged by size:
3036 300 195
PasI CC'CwG_GG
Cuts at: 0 3235 3531
Size: 3235 296
PflMI CCAn_nnn'nTGG
Cuts at: 0 1935 2980 3531
Size: 1935 1045 551
Fragments arranged by size:
1935 1045 551
PfoI T'CCnGG_A
Cuts at: 0 2483 3531
Size: 2483 1048
PpuMI rG'GwC_Cy
Cuts at: 0 375 3531
Size: 375 3156
PspOMI G'GGCC_C
Cuts at: 0 2659 3531
Size: 2659 872
PspXI vC'TCGA_Gb
Cuts at: 0 3416 3531
Size: 3416 115
PstI C_TGCA'G
Cuts at: 0 1058 1774 3531
Size: 1058 716 1757
Fragments arranged by size:
1757 1058 716
PvuII CAG'CTG
Cuts at: 0 504 1986 2461 3055 3531
Size: 504 1482 475 594 476
Fragments arranged by size:
1482 594 504 476 475
SacI G_AGCT'C
Cuts at: 0 1262 1862 2355 2588 3531
Size: 1262 600 493 233 943
Fragments arranged by size:
1262 943 600 493 233
SfcI C'TryA_G
Cuts at: 0 1054 1282 1770 2343 3531
Size: 1054 228 488 573 1188
Fragments arranged by size:
1188 1054 573 488 228
SmlI C'TyrA_G
Cuts at: 0 460 889 932 997 3416 3531
Size: 460 429 43 65 2419 115
Fragments arranged by size:
2419 460 429 115 65 43
SphI G_CATG'C
Cuts at: 0 495 3531
Size: 495 3036
StyI C'CwwG_G
Cuts at: 0 15 2611 3092 3531
Size: 15 2596 481 439
Fragments arranged by size:
2596 481 439 15
SwaI ATTT'AAAT
Cuts at: 0 1361 3531
Size: 1361 2170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1363 2702 3483 3531
Size: 1363 1339 781 48
Fragments arranged by size:
1363 1339 781 48
TatI w'GTAC_w
Cuts at: 0 801 2169 2247 3531
Size: 801 1368 78 1284
Fragments arranged by size:
1368 1284 801 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2808 2840 3231 3263 3462 3494 3531
Size: 2808 32 391 32 199 32 37
Fragments arranged by size:
2808 391 199 37 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3033 3531
Size: 3033 498
XbaI T'CTAG_A
Cuts at: 0 2556 3531
Size: 2556 975
XhoI C'TCGA_G
Cuts at: 0 3416 3531
Size: 3416 115
XmnI GAAnn'nnTTC
Cuts at: 0 1073 1145 2479 2670 3531
Size: 1073 72 1334 191 861
Fragments arranged by size:
1334 1073 861 191 72
ZraI GAC'GTC
Cuts at: 0 2402 3531
Size: 2402 1129
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AvaI BanI BanII
BbsI BbvCI BciVI BclI BglII Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtsI DraI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII MfeI MmeI
MslI MspA1I NcoI NspI PasI PflMI PfoI PpuMI
PspOMI PspXI PstI PvuII SacI SfcI SmlI SphI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmnI ZraI
Enzymes that do not cut:
Acc65I AflII AflIII AhdI AleI AloI AscI AsiSI
AvrII BaeI BamHI BbeI BcgI BfrBI BglI BlpI
BmgBI BmtI BsaAI BsaBI BsiEI BsiWI BspEI BspHI
BsrGI BssHII BstEII BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KasI KpnI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
XcmI XmaI