Restriction map for
pF1KA1282
(Linear) (Six-base) MAPSORT of: KIAA1282.seq Check: 4808 from: 1 to: 3249
KIAA1282.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 548 1724 1950 2661 3249
Size: 548 1176 226 711 588
Fragments arranged by size:
1176 711 588 548 226
AccI GT'mk_AC
Cuts at: 0 128 3249
Size: 128 3121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1021 2058 3249
Size: 1021 1037 1191
Fragments arranged by size:
1191 1037 1021
AfeI AGC'GCT
Cuts at: 0 941 3249
Size: 941 2308
AflIII A'CryG_T
Cuts at: 0 977 1409 3249
Size: 977 432 1840
Fragments arranged by size:
1840 977 432
AgeI A'CCGG_T
Cuts at: 0 1049 3249
Size: 1049 2200
AleI CACnn'nnGTG
Cuts at: 0 850 1393 3249
Size: 850 543 1856
Fragments arranged by size:
1856 850 543
AlwNI CAG_nnn'CTG
Cuts at: 0 788 1203 2016 2676 2811 3094 3249
Size: 788 415 813 660 135 283 155
Fragments arranged by size:
813 788 660 415 283 155 135
ApaI G_GGCC'C
Cuts at: 0 424 993 2215 2409 2606 3086 3249
Size: 424 569 1222 194 197 480 163
Fragments arranged by size:
1222 569 480 424 197 194 163
ApoI r'AATT_y
Cuts at: 0 834 3249
Size: 834 2415
AvaI C'yCGr_G
Cuts at: 0 167 2056 2756 2963 3249
Size: 167 1889 700 207 286
Fragments arranged by size:
1889 700 286 207 167
AvrII C'CTAG_G
Cuts at: 0 1914 3249
Size: 1914 1335
BanI G'GyrC_C
Cuts at: 0 24 455 536 1465 1897 2730 2916 3249
Size: 24 431 81 929 432 833 186 333
Fragments arranged by size:
929 833 432 431 333 186 81 24
BanII G_rGCy'C
Cuts at: 0 116 233 314 424 761 993 2099 2215
Size: 116 117 81 110 337 232 1106 116
Cuts at: 2215 2246 2409 2464 2606 2953 3005 3035 3086
Size: 31 163 55 142 347 52 30 51
Cuts at: 3086 3131 3249
Size: 45 118
Fragments arranged by size:
1106 347 337 232 163 142 118 117
116 116 110 81 55 52 51 45
31 30
BbeI G_GCGC'C
Cuts at: 0 28 459 1469 2734 3249
Size: 28 431 1010 1265 515
Fragments arranged by size:
1265 1010 515 431 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 1009 2492 3249
Size: 1009 1483 757
Fragments arranged by size:
1483 1009 757
BbvCI CC'TCA_GC
Cuts at: 0 173 2286 2378 3107 3249
Size: 173 2113 92 729 142
Fragments arranged by size:
2113 729 173 142 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1887 1921 2026 2060 2686 2720 3249
Size: 1887 34 105 34 626 34 529
Fragments arranged by size:
1887 626 529 105 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1597 3249
Size: 1597 1652
BglI GCCn_nnn'nGGC
Cuts at: 0 106 464 1636 1849 2537 3249
Size: 106 358 1172 213 688 712
Fragments arranged by size:
1172 712 688 358 213 106
BglII A'GATC_T
Cuts at: 0 1436 2454 3249
Size: 1436 1018 795
Fragments arranged by size:
1436 1018 795
BlpI GC'TnA_GC
Cuts at: 0 284 2098 3249
Size: 284 1814 1151
Fragments arranged by size:
1814 1151 284
Bme1580I G_kGCm'C
Cuts at: 0 424 474 539 666 728 993 1779 2215
Size: 424 50 65 127 62 265 786 436
Cuts at: 2215 2409 2450 2606 2755 2782 3086 3249
Size: 194 41 156 149 27 304 163
Fragments arranged by size:
786 436 424 304 265 194 163 156
149 127 65 62 50 41 27
BmgBI CAC'GTC
Cuts at: 0 1983 2311 3249
Size: 1983 328 938
Fragments arranged by size:
1983 938 328
BmrI ACTGGGnnnn_n'
Cuts at: 0 755 1125 2834 3089 3249
Size: 755 370 1709 255 160
Fragments arranged by size:
1709 755 370 255 160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2113 3249
Size: 2113 1136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1237 1274 1339 1636 2203 2232 3143 3199
Size: 1237 37 65 297 567 29 911 56
Cuts at: 3199 3249
Size: 50
Fragments arranged by size:
1237 911 567 297 65 56 50 37
29
Bpu10I CC'TnA_GC
Cuts at: 0 173 2152 2286 2378 3107 3249
Size: 173 1979 134 92 729 142
Fragments arranged by size:
1979 729 173 142 134 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1585 1873 2889 3249
Size: 1585 288 1016 360
Fragments arranged by size:
1585 1016 360 288
BsaI GGTCTCn'nnnn_
Cuts at: 0 2226 3011 3249
Size: 2226 785 238
Fragments arranged by size:
2226 785 238
BsaHI Gr'CG_yC
Cuts at: 0 25 456 1466 2731 3249
Size: 25 431 1010 1265 518
Fragments arranged by size:
1265 1010 518 431 25
BsaWI w'CCGG_w
Cuts at: 0 299 629 1049 3249
Size: 299 330 420 2200
Fragments arranged by size:
2200 420 330 299
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1345 1375 2131 2161 2734 2764 3249
Size: 1345 30 756 30 573 30 485
Fragments arranged by size:
1345 756 573 485 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2200 2268 2894 3044 3249
Size: 2200 68 626 150 205
Fragments arranged by size:
2200 626 205 150 68
BseYI C'CCAG_C
Cuts at: 0 90 781 1326 2116 2226 2232 2605 2781
Size: 90 691 545 790 110 6 373 176
Cuts at: 2781 2853 2937 3034 3249
Size: 72 84 97 215
Fragments arranged by size:
790 691 545 373 215 176 110 97
90 84 72 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1434 1702 1791 2017 2256 2737 3249
Size: 1434 268 89 226 239 481 512
Fragments arranged by size:
1434 512 481 268 239 226 89
BsiHKAI G_wGCw'C
Cuts at: 0 233 269 521 748 1889 1907 2099 2953
Size: 233 36 252 227 1141 18 192 854
Cuts at: 2953 3249
Size: 296
Fragments arranged by size:
1141 854 296 252 233 227 192 36
18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2948 3249
Size: 2948 301
Bsp1286I G_dGCh'C
Cuts at: 0 116 233 269 314 424 474 521 539
Size: 116 117 36 45 110 50 47 18
Cuts at: 539 666 728 748 761 993 1779 1889 1907
Size: 127 62 20 13 232 786 110 18
Cuts at: 1907 2099 2215 2246 2409 2450 2464 2606 2755
Size: 192 116 31 163 41 14 142 149
Cuts at: 2755 2782 2953 3005 3035 3086 3131 3249
Size: 27 171 52 30 51 45 118
Fragments arranged by size:
786 232 192 171 163 149 142 127
118 117 116 116 110 110 62 52
51 50 47 45 45 41 36 31
30 27 20 18 18 14 13
BspEI T'CCGG_A
Cuts at: 0 629 3249
Size: 629 2620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 548 962 1568 1724 1950 2661 3249
Size: 548 414 606 156 226 711 588
Fragments arranged by size:
711 606 588 548 414 226 156
BsrBI CCG'CTC
Cuts at: 0 308 2156 2579 3249
Size: 308 1848 423 670
Fragments arranged by size:
1848 670 423 308
BsrFI r'CCGG_y
Cuts at: 0 3 452 497 1049 3249
Size: 3 449 45 552 2200
Fragments arranged by size:
2200 552 449 45 3
BssHII G'CGCG_C
Cuts at: 0 1695 3249
Size: 1695 1554
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1611 2016 2676 3249
Size: 1611 405 660 573
Fragments arranged by size:
1611 660 573 405
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3208 3249
Size: 3208 41
BstYI r'GATC_y
Cuts at: 0 245 1436 2454 3249
Size: 245 1191 1018 795
Fragments arranged by size:
1191 1018 795 245
Bsu36I CC'TnA_GG
Cuts at: 0 3012 3249
Size: 3012 237
BtgI C'CryG_G
Cuts at: 0 122 771 800 1039 1070 1761 1877 2216
Size: 122 649 29 239 31 691 116 339
Cuts at: 2216 2401 2437 2598 3201 3249
Size: 185 36 161 603 48
Fragments arranged by size:
691 649 603 339 239 185 161 122
116 48 36 31 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 36 918 1689 1853 3249
Size: 36 882 771 164 1396
Fragments arranged by size:
1396 882 771 164 36
BtsI GCAGTG_nn'
Cuts at: 0 1081 2017 3249
Size: 1081 936 1232
Fragments arranged by size:
1232 1081 936
DraIII CAC_nnn'GTG
Cuts at: 0 1482 3249
Size: 1482 1767
DrdI GACnn_nn'nnGTC
Cuts at: 0 2661 3249
Size: 2661 588
EaeI y'GGCC_r
Cuts at: 0 5 450 465 797 1091 1143 1269 1502
Size: 5 445 15 332 294 52 126 233
Cuts at: 1502 1754 3249
Size: 252 1495
Fragments arranged by size:
1495 445 332 294 252 233 126 52
15 5
EarI CTCTTCn'nnn_
Cuts at: 0 121 298 379 817 3249
Size: 121 177 81 438 2432
Fragments arranged by size:
2432 438 177 121 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 226 519 1404 3249
Size: 226 293 885 1845
Fragments arranged by size:
1845 885 293 226
EcoICRI GAG'CTC
Cuts at: 0 231 2097 2951 3249
Size: 231 1866 854 298
Fragments arranged by size:
1866 854 298 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1021 1237 1274 1339 1636 2058 2203 2232
Size: 1021 216 37 65 297 422 145 29
Cuts at: 2232 3143 3199 3249
Size: 911 56 50
Fragments arranged by size:
1021 911 422 297 216 145 65 56
50 37 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1720 1918 2333 3011 3249
Size: 1720 198 415 678 238
Fragments arranged by size:
1720 678 415 238 198
EcoO109I rG'GnC_Cy
Cuts at: 0 15 255 420 421 807 989 1728 1851
Size: 15 240 165 1 386 182 739 123
Cuts at: 1851 2211 2212 2406 2899 2932 3147 3165 3249
Size: 360 1 194 493 33 215 18 84
Fragments arranged by size:
739 493 386 360 240 215 194 182
165 123 84 33 18 15 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 657 3249
Size: 657 2592
FspI TGC'GCA
Cuts at: 0 1345 3249
Size: 1345 1904
HaeII r_GCGC'y
Cuts at: 0 28 459 943 1070 1352 1469 2734 3249
Size: 28 431 484 127 282 117 1265 515
Fragments arranged by size:
1265 515 484 431 282 127 117 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1039 1071 2131 2163 2230 2262 3010 3042
Size: 1039 32 1060 32 67 32 748 32
Cuts at: 3042 3249
Size: 207
Fragments arranged by size:
1060 1039 748 207 67 32 32 32
32
HincII GTy'rAC
Cuts at: 0 975 3249
Size: 975 2274
HindIII A'AGCT_T
Cuts at: 0 2638 3249
Size: 2638 611
KasI G'GCGC_C
Cuts at: 0 24 455 1465 2730 3249
Size: 24 431 1010 1265 519
Fragments arranged by size:
1265 1010 519 431 24
MscI TGG'CCA
Cuts at: 0 1145 3249
Size: 1145 2104
MslI CAynn'nnrTG
Cuts at: 0 850 1093 1393 1708 1882 3249
Size: 850 243 300 315 174 1367
Fragments arranged by size:
1367 850 315 300 243 174
MspA1I CmG'CkG
Cuts at: 0 186 773 1041 1149 1252 1737 1763 2022
Size: 186 587 268 108 103 485 26 259
Cuts at: 2022 2236 2290 2857 3249
Size: 214 54 567 392
Fragments arranged by size:
587 567 485 392 268 259 214 186
108 103 54 26
NaeI GCC'GGC
Cuts at: 0 5 454 3249
Size: 5 449 2795
Fragments arranged by size:
2795 449 5
NarI GG'CG_CC
Cuts at: 0 25 456 1466 2731 3249
Size: 25 431 1010 1265 518
Fragments arranged by size:
1265 1010 518 431 25
NcoI C'CATG_G
Cuts at: 0 1877 2437 2598 3201 3249
Size: 1877 560 161 603 48
Fragments arranged by size:
1877 603 560 161 48
NgoMIV G'CCGG_C
Cuts at: 0 3 452 3249
Size: 3 449 2797
Fragments arranged by size:
2797 449 3
NspI r_CATG'y
Cuts at: 0 964 1521 1614 3249
Size: 964 557 93 1635
Fragments arranged by size:
1635 964 557 93
PasI CC'CwG_GG
Cuts at: 0 2465 2927 3161 3249
Size: 2465 462 234 88
Fragments arranged by size:
2465 462 234 88
PflMI CCAn_nnn'nTGG
Cuts at: 0 2438 3177 3249
Size: 2438 739 72
Fragments arranged by size:
2438 739 72
PfoI T'CCnGG_A
Cuts at: 0 41 329 3249
Size: 41 288 2920
Fragments arranged by size:
2920 288 41
PpuMI rG'GwC_Cy
Cuts at: 0 807 1728 2932 3165 3249
Size: 807 921 1204 233 84
Fragments arranged by size:
1204 921 807 233 84
PshAI GACnn'nnGTC
Cuts at: 0 2697 3249
Size: 2697 552
PspOMI G'GGCC_C
Cuts at: 0 420 989 2211 2405 2602 3082 3249
Size: 420 569 1222 194 197 480 167
Fragments arranged by size:
1222 569 480 420 197 194 167
PspXI vC'TCGA_Gb
Cuts at: 0 2963 3249
Size: 2963 286
PstI C_TGCA'G
Cuts at: 0 545 1199 1288 1676 1736 1850 2012 2021
Size: 545 654 89 388 60 114 162 9
Cuts at: 2021 2133 2681 2699 2807 2849 3249
Size: 112 548 18 108 42 400
Fragments arranged by size:
654 548 545 400 388 162 114 112
108 89 60 42 18 9
PvuII CAG'CTG
Cuts at: 0 1252 1737 2022 2236 2290 2857 3249
Size: 1252 485 285 214 54 567 392
Fragments arranged by size:
1252 567 485 392 285 214 54
SacI G_AGCT'C
Cuts at: 0 233 2099 2953 3249
Size: 233 1866 854 296
Fragments arranged by size:
1866 854 296 233
SacII CC_GC'GG
Cuts at: 0 774 1042 1764 3249
Size: 774 268 722 1485
Fragments arranged by size:
1485 774 722 268
SanDI GG'GwC_CC
Cuts at: 0 2932 3165 3249
Size: 2932 233 84
Fragments arranged by size:
2932 233 84
SapI GCTCTTCn'nnn_
Cuts at: 0 121 298 3249
Size: 121 177 2951
Fragments arranged by size:
2951 177 121
SbfI CC_TGCA'GG
Cuts at: 0 1850 3249
Size: 1850 1399
ScaI AGT'ACT
Cuts at: 0 1621 3249
Size: 1621 1628
SexAI A'CCwGG_T
Cuts at: 0 913 1232 3249
Size: 913 319 2017
Fragments arranged by size:
2017 913 319
SfcI C'TryA_G
Cuts at: 0 541 729 1195 1284 1672 1732 1846 2008
Size: 541 188 466 89 388 60 114 162
Cuts at: 2008 2017 2129 2677 2695 2803 2845 3249
Size: 9 112 548 18 108 42 404
Fragments arranged by size:
548 541 466 404 388 188 162 114
112 108 89 60 42 18 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2537 3249
Size: 2537 712
SfoI GGC'GCC
Cuts at: 0 26 457 1467 2732 3249
Size: 26 431 1010 1265 517
Fragments arranged by size:
1265 1010 517 431 26
SmaI CCC'GGG
Cuts at: 0 169 3249
Size: 169 3080
SmlI C'TyrA_G
Cuts at: 0 1600 1888 2868 2963 3249
Size: 1600 288 980 95 286
Fragments arranged by size:
1600 980 288 286 95
SphI G_CATG'C
Cuts at: 0 1614 3249
Size: 1614 1635
StyI C'CwwG_G
Cuts at: 0 554 1833 1877 1914 2437 2598 3201 3249
Size: 554 1279 44 37 523 161 603 48
Fragments arranged by size:
1279 603 554 523 161 48 44 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2101 3249
Size: 2101 1148
TatI w'GTAC_w
Cuts at: 0 611 980 1061 1619 3249
Size: 611 369 81 558 1630
Fragments arranged by size:
1630 611 558 369 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2734 2766 3249
Size: 2734 32 483
Fragments arranged by size:
2734 483 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 708 3249
Size: 708 2541
XhoI C'TCGA_G
Cuts at: 0 2963 3249
Size: 2963 286
XmaI C'CCGG_G
Cuts at: 0 167 3249
Size: 167 3082
XmnI GAAnn'nnTTC
Cuts at: 0 37 1438 3249
Size: 37 1401 1811
Fragments arranged by size:
1811 1401 37
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AleI AlwNI
ApaI ApoI AvaI AvrII BanI BanII BbeI BbsI
BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII HindIII KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PshAI
PspOMI PspXI PstI PvuII SacI SacII SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SmaI SmlI
SphI StyI TaqII TatI TstI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AhdI AlfI AloI ApaLI
AscI AseI AsiSI BaeI BamHI BclI BfrBI BmtI
BsaAI BsaBI BsiEI BsiWI BsmI BspHI BsrDI BsrGI
BssSI BstBI BstEII BstZ17I ClaI CspCI DraI EagI
EcoRI EcoRV FseI FspAI HpaI KpnI MfeI MluI
MmeI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PsiI PsrI PvuI RsrII SalI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI ZraI