Restriction map for
pF1KSDA1284
(Linear) (Six-base) MAPSORT of: KIAA1284.seq Check: 4695 from: 1 to: 2826
KIAA1284.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 756 2826
Size: 756 2070
AccI GT'mk_AC
Cuts at: 0 1534 1619 2287 2826
Size: 1534 85 668 539
Fragments arranged by size:
1534 668 539 85
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1196 1312 1757 1775 2826
Size: 1022 174 116 445 18 1051
Fragments arranged by size:
1051 1022 445 174 116 18
AfeI AGC'GCT
Cuts at: 0 379 2826
Size: 379 2447
AflII C'TTAA_G
Cuts at: 0 2266 2826
Size: 2266 560
AhdI GACnn_n'nnGTC
Cuts at: 0 2378 2826
Size: 2378 448
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1394 1603 2826
Size: 1394 209 1223
Fragments arranged by size:
1394 1223 209
AlwNI CAG_nnn'CTG
Cuts at: 0 2596 2826
Size: 2596 230
ApoI r'AATT_y
Cuts at: 0 347 977 1118 1234 2499 2826
Size: 347 630 141 116 1265 327
Fragments arranged by size:
1265 630 347 327 141 116
AvaI C'yCGr_G
Cuts at: 0 111 2826
Size: 111 2715
BanII G_rGCy'C
Cuts at: 0 37 47 2236 2826
Size: 37 10 2189 590
Fragments arranged by size:
2189 590 37 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 451 1541 2603 2826
Size: 451 1090 1062 223
Fragments arranged by size:
1090 1062 451 223
BbvCI CC'TCA_GC
Cuts at: 0 126 1700 2486 2826
Size: 126 1574 786 340
Fragments arranged by size:
1574 786 340 126
BciVI GTATCCnnnnn_n'
Cuts at: 0 426 901 2826
Size: 426 475 1925
Fragments arranged by size:
1925 475 426
BclI T'GATC_A
Cuts at: 0 1676 2673 2826
Size: 1676 997 153
Fragments arranged by size:
1676 997 153
BfrBI ATG'CAT
Cuts at: 0 790 2826
Size: 790 2036
BglI GCCn_nnn'nGGC
Cuts at: 0 10 162 2826
Size: 10 152 2664
Fragments arranged by size:
2664 152 10
BlpI GC'TnA_GC
Cuts at: 0 1367 2826
Size: 1367 1459
BmrI ACTGGGnnnn_n'
Cuts at: 0 1082 1355 2715 2826
Size: 1082 273 1360 111
Fragments arranged by size:
1360 1082 273 111
BmtI G_CTAG'C
Cuts at: 0 1939 2826
Size: 1939 887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 606 1433 1841 1931 2826
Size: 71 535 827 408 90 895
Fragments arranged by size:
895 827 535 408 90 71
Bpu10I CC'TnA_GC
Cuts at: 0 126 1700 2486 2826
Size: 126 1574 786 340
Fragments arranged by size:
1574 786 340 126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 316 520 1713 2726 2826
Size: 172 144 204 1193 1013 100
Fragments arranged by size:
1193 1013 204 172 144 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 47 2576 2826
Size: 47 2529 250
Fragments arranged by size:
2529 250 47
BsaAI yAC'GTr
Cuts at: 0 1993 2826
Size: 1993 833
BsaWI w'CCGG_w
Cuts at: 0 595 1418 1746 2199 2826
Size: 595 823 328 453 627
Fragments arranged by size:
823 627 595 453 328
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 443 473 866 896 2826
Size: 443 30 393 30 1930
Fragments arranged by size:
1930 443 393 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 905 2826
Size: 905 1921
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1409 2549 2826
Size: 196 1213 1140 277
Fragments arranged by size:
1213 1140 277 196
BsiHKAI G_wGCw'C
Cuts at: 0 37 47 1326 1368 2826
Size: 37 10 1279 42 1458
Fragments arranged by size:
1458 1279 42 37 10
BsmI GAATG_Cn'
Cuts at: 0 749 1344 2826
Size: 749 595 1482
Fragments arranged by size:
1482 749 595
Bsp1286I G_dGCh'C
Cuts at: 0 37 47 1326 1368 2236 2826
Size: 37 10 1279 42 868 590
Fragments arranged by size:
1279 868 590 42 37 10
BspEI T'CCGG_A
Cuts at: 0 1746 2826
Size: 1746 1080
BspHI T'CATG_A
Cuts at: 0 2763 2826
Size: 2763 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 756 2826
Size: 756 2070
BsrBI CCG'CTC
Cuts at: 0 665 2826
Size: 665 2161
BsrDI GCAATG_nn'
Cuts at: 0 405 2826
Size: 405 2421
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1492 2826
Size: 1492 1334
BstXI CCAn_nnnn'nTGG
Cuts at: 0 901 1906 2826
Size: 901 1005 920
Fragments arranged by size:
1005 920 901
BstYI r'GATC_y
Cuts at: 0 649 2143 2826
Size: 649 1494 683
Fragments arranged by size:
1494 683 649
BstZ17I GTA'TAC
Cuts at: 0 1535 1620 2826
Size: 1535 85 1206
Fragments arranged by size:
1535 1206 85
DraI TTT'AAA
Cuts at: 0 1106 2802 2826
Size: 1106 1696 24
Fragments arranged by size:
1696 1106 24
DraIII CAC_nnn'GTG
Cuts at: 0 1051 2826
Size: 1051 1775
EarI CTCTTCn'nnn_
Cuts at: 0 66 655 872 962 1193 1898 2297 2447
Size: 66 589 217 90 231 705 399 150
Cuts at: 2447 2549 2826
Size: 102 277
Fragments arranged by size:
705 589 399 277 231 217 150 102
90 66
EcoICRI GAG'CTC
Cuts at: 0 35 45 2826
Size: 35 10 2781
Fragments arranged by size:
2781 35 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 606 1022 1196 1312 1433 1757 1775
Size: 71 535 416 174 116 121 324 18
Cuts at: 1775 1841 1931 2826
Size: 66 90 895
Fragments arranged by size:
895 535 416 324 174 121 116 90
71 66 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2452 2826
Size: 2452 374
EcoO109I rG'GnC_Cy
Cuts at: 0 2258 2328 2826
Size: 2258 70 498
Fragments arranged by size:
2258 498 70
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1204 2826
Size: 1204 1622
FspI TGC'GCA
Cuts at: 0 1830 2826
Size: 1830 996
HaeII r_GCGC'y
Cuts at: 0 381 1355 2826
Size: 381 974 1471
Fragments arranged by size:
1471 974 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 443 475 801 833 1566 1598 2364 2396
Size: 443 32 326 32 733 32 766 32
Cuts at: 2396 2826
Size: 430
Fragments arranged by size:
766 733 443 430 326 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2819 2826
Size: 2819 7
HindIII A'AGCT_T
Cuts at: 0 538 862 2826
Size: 538 324 1964
Fragments arranged by size:
1964 538 324
HpaI GTT'AAC
Cuts at: 0 2819 2826
Size: 2819 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 486 537 866 1737 2092 2562 2826
Size: 486 51 329 871 355 470 264
Fragments arranged by size:
871 486 470 355 329 264 51
MspA1I CmG'CkG
Cuts at: 0 468 1902 2826
Size: 468 1434 924
Fragments arranged by size:
1434 924 468
NdeI CA'TA_TG
Cuts at: 0 792 1681 2826
Size: 792 889 1145
Fragments arranged by size:
1145 889 792
NheI G'CTAG_C
Cuts at: 0 1935 2826
Size: 1935 891
NsiI A_TGCA'T
Cuts at: 0 792 2826
Size: 792 2034
NspI r_CATG'y
Cuts at: 0 1059 2075 2826
Size: 1059 1016 751
Fragments arranged by size:
1059 1016 751
PfoI T'CCnGG_A
Cuts at: 0 753 1851 2826
Size: 753 1098 975
Fragments arranged by size:
1098 975 753
PmlI CAC'GTG
Cuts at: 0 1993 2826
Size: 1993 833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 314 346 2826
Size: 314 32 2480
Fragments arranged by size:
2480 314 32
PpuMI rG'GwC_Cy
Cuts at: 0 2258 2328 2826
Size: 2258 70 498
Fragments arranged by size:
2258 498 70
PshAI GACnn'nnGTC
Cuts at: 0 451 2826
Size: 451 2375
PstI C_TGCA'G
Cuts at: 0 1488 2826
Size: 1488 1338
PvuII CAG'CTG
Cuts at: 0 1902 2826
Size: 1902 924
SacI G_AGCT'C
Cuts at: 0 37 47 2826
Size: 37 10 2779
Fragments arranged by size:
2779 37 10
SapI GCTCTTCn'nnn_
Cuts at: 0 655 962 2826
Size: 655 307 1864
Fragments arranged by size:
1864 655 307
ScaI AGT'ACT
Cuts at: 0 1394 2826
Size: 1394 1432
SfcI C'TryA_G
Cuts at: 0 940 1484 2130 2826
Size: 940 544 646 696
Fragments arranged by size:
940 696 646 544
SmlI C'TyrA_G
Cuts at: 0 151 331 535 1728 2266 2741 2826
Size: 151 180 204 1193 538 475 85
Fragments arranged by size:
1193 538 475 204 180 151 85
SpeI A'CTAG_T
Cuts at: 0 1087 2826
Size: 1087 1739
StyI C'CwwG_G
Cuts at: 0 1592 2826
Size: 1592 1234
TatI w'GTAC_w
Cuts at: 0 1392 1806 2826
Size: 1392 414 1020
Fragments arranged by size:
1392 1020 414
Tth111I GACn'n_nGTC
Cuts at: 0 837 2770 2826
Size: 837 1933 56
Fragments arranged by size:
1933 837 56
XmnI GAAnn'nnTTC
Cuts at: 0 1181 1694 1742 2826
Size: 1181 513 48 1084
Fragments arranged by size:
1181 1084 513 48
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AhdI AlfI AlwNI
ApoI AvaI BanII BbsI BbvCI BciVI BclI BfrBI
BglI BlpI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BstAPI BstXI BstYI
BstZ17I DraI DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII HindIII HpaI MmeI
MspA1I NdeI NheI NsiI NspI PfoI PmlI PpiI
PpuMI PshAI PstI PvuII SacI SapI ScaI SfcI
SmlI SpeI StyI TatI Tth111I XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflIII AgeI AleI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BcgI BglII Bme1580I BmgBI BplI BsaBI BsaHI
BseYI BsiEI BsiWI BsmBI BsrFI BsrGI BssHII BssSI
BstBI BstEII Bsu36I BtgI BtgZI BtsI ClaI CspCI
DrdI EaeI EagI EciI EcoRI EcoRV FseI FspAI
KasI KpnI MfeI MluI MscI MslI NaeI NarI
NcoI NgoMIV NotI NruI PacI PasI PciI PflMI
PmeI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI SspI StuI SwaI TaqII
TstI XbaI XcmI XhoI XmaI ZraI