Restriction map for
pF1KA1286
(Linear) (Six-base) MAPSORT of: KIAA1286.seq Check: 5843 from: 1 to: 3615
KIAA1286.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2135 3528 3615
Size: 2135 1393 87
Fragments arranged by size:
2135 1393 87
AccI GT'mk_AC
Cuts at: 0 128 563 3615
Size: 128 435 3052
Fragments arranged by size:
3052 435 128
Acc65I G'GTAC_C
Cuts at: 0 2749 3615
Size: 2749 866
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 455 911 1258 1666 2254 3241 3376
Size: 206 249 456 347 408 588 987 135
Cuts at: 3376 3615
Size: 239
Fragments arranged by size:
987 588 456 408 347 249 239 206
135
AflIII A'CryG_T
Cuts at: 0 1051 3615
Size: 1051 2564
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2143 3550 3615
Size: 2143 1407 65
Fragments arranged by size:
2143 1407 65
AlwNI CAG_nnn'CTG
Cuts at: 0 2343 2939 3385 3615
Size: 2343 596 446 230
Fragments arranged by size:
2343 596 446 230
ApaI G_GGCC'C
Cuts at: 0 612 864 2211 2289 2417 3587 3615
Size: 612 252 1347 78 128 1170 28
Fragments arranged by size:
1347 1170 612 252 128 78 28
ApaLI G'TGCA_C
Cuts at: 0 1055 3615
Size: 1055 2560
ApoI r'AATT_y
Cuts at: 0 1882 3615
Size: 1882 1733
AseI AT'TA_AT
Cuts at: 0 2585 3615
Size: 2585 1030
AvaI C'yCGr_G
Cuts at: 0 83 2110 2131 3128 3299 3615
Size: 83 2027 21 997 171 316
Fragments arranged by size:
2027 997 316 171 83 21
BamHI G'GATC_C
Cuts at: 0 884 1824 3615
Size: 884 940 1791
Fragments arranged by size:
1791 940 884
BanI G'GyrC_C
Cuts at: 0 829 1114 2749 3615
Size: 829 285 1635 866
Fragments arranged by size:
1635 866 829 285
BanII G_rGCy'C
Cuts at: 0 612 864 1073 1091 2211 2289 2417 2519
Size: 612 252 209 18 1120 78 128 102
Cuts at: 2519 2567 2645 3587 3615
Size: 48 78 942 28
Fragments arranged by size:
1120 942 612 252 209 128 102 78
78 48 28 18
BbeI G_GCGC'C
Cuts at: 0 833 3615
Size: 833 2782
BbsI GAAGACnn'nnnn_
Cuts at: 0 443 638 1627 2156 2180 2471 3212 3482
Size: 443 195 989 529 24 291 741 270
Cuts at: 3482 3615
Size: 133
Fragments arranged by size:
989 741 529 443 291 270 195 133
24
BciVI GTATCCnnnnn_n'
Cuts at: 0 2400 3615
Size: 2400 1215
BclI T'GATC_A
Cuts at: 0 3278 3615
Size: 3278 337
BfrBI ATG'CAT
Cuts at: 0 319 3615
Size: 319 3296
BglI GCCn_nnn'nGGC
Cuts at: 0 1112 2412 2523 3615
Size: 1112 1300 111 1092
Fragments arranged by size:
1300 1112 1092 111
BglII A'GATC_T
Cuts at: 0 1457 3615
Size: 1457 2158
BlpI GC'TnA_GC
Cuts at: 0 1598 3615
Size: 1598 2017
Bme1580I G_kGCm'C
Cuts at: 0 527 612 857 864 1059 1334 2122 2211
Size: 527 85 245 7 195 275 788 89
Cuts at: 2211 2289 2417 3137 3587 3615
Size: 78 128 720 450 28
Fragments arranged by size:
788 720 527 450 275 245 195 128
89 85 78 28 7
BmgBI CAC'GTC
Cuts at: 0 2178 3351 3615
Size: 2178 1173 264
Fragments arranged by size:
2178 1173 264
BmrI ACTGGGnnnn_n'
Cuts at: 0 867 2531 3615
Size: 867 1664 1084
Fragments arranged by size:
1664 1084 867
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 26 1173 1738 2138 2928 3412 3615
Size: 26 1147 565 400 790 484 203
Fragments arranged by size:
1147 790 565 484 400 203 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1786 1948 2266 2458 2533 2863 2870 3407
Size: 1786 162 318 192 75 330 7 537
Cuts at: 3407 3615
Size: 208
Fragments arranged by size:
1786 537 330 318 208 192 162 75
7
Bpu10I CC'TnA_GC
Cuts at: 0 3177 3568 3615
Size: 3177 391 47
Fragments arranged by size:
3177 391 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 592 1283 1306 1878 3478 3615
Size: 52 540 691 23 572 1600 137
Fragments arranged by size:
1600 691 572 540 137 52 23
BsaI GGTCTCn'nnnn_
Cuts at: 0 82 1474 3055 3209 3615
Size: 82 1392 1581 154 406
Fragments arranged by size:
1581 1392 406 154 82
BsaHI Gr'CG_yC
Cuts at: 0 141 734 830 3091 3615
Size: 141 593 96 2261 524
Fragments arranged by size:
2261 593 524 141 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1387 1417 1633 1663 2149 2179 2928 2958
Size: 1387 30 216 30 486 30 749 30
Cuts at: 2958 3223 3253 3615
Size: 265 30 362
Fragments arranged by size:
1387 749 486 362 265 216 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 487 1270 1654 1969 1978 2470 2489 3615
Size: 487 783 384 315 9 492 19 1126
Fragments arranged by size:
1126 783 492 487 384 315 19 9
BseYI C'CCAG_C
Cuts at: 0 110 349 1066 2969 3360 3615
Size: 110 239 717 1903 391 255
Fragments arranged by size:
1903 717 391 255 239 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1649 1814 2248 2513 3298 3556 3615
Size: 1022 627 165 434 265 785 258 59
Fragments arranged by size:
1022 785 627 434 265 258 165 59
BsiEI CG_ry'CG
Cuts at: 0 2747 3615
Size: 2747 868
BsiHKAI G_wGCw'C
Cuts at: 0 76 1059 2645 3615
Size: 76 983 1586 970
Fragments arranged by size:
1586 983 970 76
BsmI GAATG_Cn'
Cuts at: 0 36 223 1039 3615
Size: 36 187 816 2576
Fragments arranged by size:
2576 816 187 36
Bsp1286I G_dGCh'C
Cuts at: 0 76 527 612 857 864 1059 1073 1091
Size: 76 451 85 245 7 195 14 18
Cuts at: 1091 1334 2122 2211 2289 2417 2519 2567 2645
Size: 243 788 89 78 128 102 48 78
Cuts at: 2645 3137 3587 3615
Size: 492 450 28
Fragments arranged by size:
788 492 451 450 245 243 195 128
102 89 85 78 78 76 48 28
18 14 7
BspHI T'CATG_A
Cuts at: 0 1076 3615
Size: 1076 2539
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 979 1725 1817 2135 2597 3528 3615
Size: 979 746 92 318 462 931 87
Fragments arranged by size:
979 931 746 462 318 92 87
BsrBI CCG'CTC
Cuts at: 0 1680 3615
Size: 1680 1935
BsrDI GCAATG_nn'
Cuts at: 0 684 3615
Size: 684 2931
BsrFI r'CCGG_y
Cuts at: 0 41 566 2746 3615
Size: 41 525 2180 869
Fragments arranged by size:
2180 869 525 41
BsrGI T'GTAC_A
Cuts at: 0 714 3615
Size: 714 2901
BstAPI GCAn_nnn'nTGC
Cuts at: 0 812 2448 2691 3615
Size: 812 1636 243 924
Fragments arranged by size:
1636 924 812 243
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1764 1899 2628 3615
Size: 1764 135 729 987
Fragments arranged by size:
1764 987 729 135
BstYI r'GATC_y
Cuts at: 0 884 1457 1824 3615
Size: 884 573 367 1791
Fragments arranged by size:
1791 884 573 367
Bsu36I CC'TnA_GG
Cuts at: 0 749 3615
Size: 749 2866
BtgI C'CryG_G
Cuts at: 0 1757 2859 3108 3210 3482 3615
Size: 1757 1102 249 102 272 133
Fragments arranged by size:
1757 1102 272 249 133 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1238 3571 3615
Size: 1238 2333 44
Fragments arranged by size:
2333 1238 44
BtsI GCAGTG_nn'
Cuts at: 0 609 1175 1637 2659 3087 3442 3615
Size: 609 566 462 1022 428 355 173
Fragments arranged by size:
1022 609 566 462 428 355 173
ClaI AT'CG_AT
Cuts at: 0 626 3284 3615
Size: 626 2658 331
Fragments arranged by size:
2658 626 331
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 807 842 3615
Size: 807 35 2773
Fragments arranged by size:
2773 807 35
DraIII CAC_nnn'GTG
Cuts at: 0 1168 2761 3615
Size: 1168 1593 854
Fragments arranged by size:
1593 1168 854
EaeI y'GGCC_r
Cuts at: 0 1172 2744 2923 3615
Size: 1172 1572 179 692
Fragments arranged by size:
1572 1172 692 179
EagI C'GGCC_G
Cuts at: 0 2744 3615
Size: 2744 871
EarI CTCTTCn'nnn_
Cuts at: 0 694 1078 1259 1265 1280 1705 2794 2897
Size: 694 384 181 6 15 425 1089 103
Cuts at: 2897 2975 3403 3415 3615
Size: 78 428 12 200
Fragments arranged by size:
1089 694 428 425 384 200 181 103
78 15 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1935 2029 2293 2492 3615
Size: 1935 94 264 199 1123
Fragments arranged by size:
1935 1123 264 199 94
EcoICRI GAG'CTC
Cuts at: 0 2643 3615
Size: 2643 972
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 455 911 1258 1666 1786 1948 2254
Size: 206 249 456 347 408 120 162 306
Cuts at: 2254 2266 2458 2533 2863 2870 3241 3376 3407
Size: 12 192 75 330 7 371 135 31
Cuts at: 3407 3615
Size: 208
Fragments arranged by size:
456 408 371 347 330 306 249 208
206 192 162 135 120 75 31 12
7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1443 1479 1813 3615
Size: 1443 36 334 1802
Fragments arranged by size:
1802 1443 334 36
EcoO109I rG'GnC_Cy
Cuts at: 0 477 608 2049 2285 2413 2414 2838 2963
Size: 477 131 1441 236 128 1 424 125
Cuts at: 2963 3306 3583 3584 3615
Size: 343 277 1 31
Fragments arranged by size:
1441 477 424 343 277 236 131 128
125 31 1 1
EcoRI G'AATT_C
Cuts at: 0 1882 3615
Size: 1882 1733
EcoRV GAT'ATC
Cuts at: 0 210 3615
Size: 210 3405
FspI TGC'GCA
Cuts at: 0 1132 3615
Size: 1132 2483
HaeII r_GCGC'y
Cuts at: 0 833 1566 2270 2910 3615
Size: 833 733 704 640 705
Fragments arranged by size:
833 733 705 704 640
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 565 597 1387 1419 1721 1753 2021 2048
Size: 565 32 790 32 302 32 268 27
Cuts at: 2048 2053 2080 2536 2568 2708 2740 3270 3302
Size: 5 27 456 32 140 32 530 32
Cuts at: 3302 3615
Size: 313
Fragments arranged by size:
790 565 530 456 313 302 268 140
32 32 32 32 32 32 27 27
5
HincII GTy'rAC
Cuts at: 0 1854 3615
Size: 1854 1761
HindIII A'AGCT_T
Cuts at: 0 3074 3615
Size: 3074 541
KasI G'GCGC_C
Cuts at: 0 829 3615
Size: 829 2786
KpnI G_GTAC'C
Cuts at: 0 2753 3615
Size: 2753 862
MspA1I CmG'CkG
Cuts at: 0 114 396 770 959 1062 1503 2035 2232
Size: 114 282 374 189 103 441 532 197
Cuts at: 2232 2431 3212 3345 3615
Size: 199 781 133 270
Fragments arranged by size:
781 532 441 374 282 270 199 197
189 133 114 103
NaeI GCC'GGC
Cuts at: 0 43 3615
Size: 43 3572
NarI GG'CG_CC
Cuts at: 0 830 3615
Size: 830 2785
NcoI C'CATG_G
Cuts at: 0 1757 2859 3615
Size: 1757 1102 756
Fragments arranged by size:
1757 1102 756
NdeI CA'TA_TG
Cuts at: 0 99 3615
Size: 99 3516
NgoMIV G'CCGG_C
Cuts at: 0 41 3615
Size: 41 3574
NsiI A_TGCA'T
Cuts at: 0 321 3615
Size: 321 3294
NspI r_CATG'y
Cuts at: 0 319 1055 3615
Size: 319 736 2560
Fragments arranged by size:
2560 736 319
PasI CC'CwG_GG
Cuts at: 0 2418 3615
Size: 2418 1197
PciI A'CATG_T
Cuts at: 0 1051 3615
Size: 1051 2564
PflMI CCAn_nnn'nTGG
Cuts at: 0 2761 2865 3615
Size: 2761 104 750
Fragments arranged by size:
2761 750 104
PfoI T'CCnGG_A
Cuts at: 0 2840 2914 3140 3615
Size: 2840 74 226 475
Fragments arranged by size:
2840 475 226 74
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3145 3177 3615
Size: 3145 32 438
Fragments arranged by size:
3145 438 32
PpuMI rG'GwC_Cy
Cuts at: 0 477 2049 2838 2963 3615
Size: 477 1572 789 125 652
Fragments arranged by size:
1572 789 652 477 125
PspOMI G'GGCC_C
Cuts at: 0 608 860 2207 2285 2413 3583 3615
Size: 608 252 1347 78 128 1170 32
Fragments arranged by size:
1347 1170 608 252 128 78 32
PstI C_TGCA'G
Cuts at: 0 543 782 1583 1817 2666 3615
Size: 543 239 801 234 849 949
Fragments arranged by size:
949 849 801 543 239 234
PvuII CAG'CTG
Cuts at: 0 2035 2232 2431 3615
Size: 2035 197 199 1184
Fragments arranged by size:
2035 1184 199 197
SacI G_AGCT'C
Cuts at: 0 2645 3615
Size: 2645 970
SacII CC_GC'GG
Cuts at: 0 3213 3615
Size: 3213 402
SapI GCTCTTCn'nnn_
Cuts at: 0 1078 1280 2897 3403 3615
Size: 1078 202 1617 506 212
Fragments arranged by size:
1617 1078 506 212 202
SexAI A'CCwGG_T
Cuts at: 0 1164 2258 3615
Size: 1164 1094 1357
Fragments arranged by size:
1357 1164 1094
SfcI C'TryA_G
Cuts at: 0 60 539 778 1461 1579 1813 2662 2818
Size: 60 479 239 683 118 234 849 156
Cuts at: 2818 2940 3615
Size: 122 675
Fragments arranged by size:
849 683 675 479 239 234 156 122
118 60
SfoI GGC'GCC
Cuts at: 0 831 3615
Size: 831 2784
SmaI CCC'GGG
Cuts at: 0 85 3301 3615
Size: 85 3216 314
Fragments arranged by size:
3216 314 85
SmlI C'TyrA_G
Cuts at: 0 67 571 1298 1321 1857 3128 3493 3615
Size: 67 504 727 23 536 1271 365 122
Fragments arranged by size:
1271 727 536 504 365 122 67 23
SphI G_CATG'C
Cuts at: 0 319 3615
Size: 319 3296
StuI AGG'CCT
Cuts at: 0 2446 3615
Size: 2446 1169
StyI C'CwwG_G
Cuts at: 0 284 1757 1949 2859 2926 3227 3445 3464
Size: 284 1473 192 910 67 301 218 19
Cuts at: 3464 3615
Size: 151
Fragments arranged by size:
1473 910 301 284 218 192 151 67
19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 93 2154 3457 3615
Size: 93 2061 1303 158
Fragments arranged by size:
2061 1303 158 93
TatI w'GTAC_w
Cuts at: 0 714 3615
Size: 714 2901
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2928 2960 3223 3255 3615
Size: 2928 32 263 32 360
Fragments arranged by size:
2928 360 263 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2836 3454 3615
Size: 2836 618 161
Fragments arranged by size:
2836 618 161
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 357 727 3615
Size: 357 370 2888
Fragments arranged by size:
2888 370 357
XhoI C'TCGA_G
Cuts at: 0 3128 3615
Size: 3128 487
XmaI C'CCGG_G
Cuts at: 0 83 3299 3615
Size: 83 3216 316
Fragments arranged by size:
3216 316 83
XmnI GAAnn'nnTTC
Cuts at: 0 3397 3615
Size: 3397 218
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII KasI
KpnI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI
PstI PvuII SacI SacII SapI SexAI SfcI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AloI
AscI AsiSI AvrII BaeI BbvCI BcgI BmtI BsaAI
BsaBI BsaWI BsiWI BsmBI BspEI BssHII BssSI BstBI
BstEII BstZ17I DraI DrdI FalI FseI FspAI HpaI
MfeI MluI MmeI MscI MslI NheI NotI NruI
PacI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI SbfI ScaI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI XbaI ZraI