Restriction map for
pF1KA1287
(Linear) (Six-base) MAPSORT of: KIAA1287.seq Check: 9914 from: 1 to: 3612
KIAA1287.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 177 3612
Size: 177 3435
AccI GT'mk_AC
Cuts at: 0 2542 3195 3612
Size: 2542 653 417
Fragments arranged by size:
2542 653 417
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 349 614 1232 1391 2491 2707 2867
Size: 28 321 265 618 159 1100 216 160
Cuts at: 2867 3612
Size: 745
Fragments arranged by size:
1100 745 618 321 265 216 160 159
28
AfeI AGC'GCT
Cuts at: 0 2919 3612
Size: 2919 693
AflIII A'CryG_T
Cuts at: 0 606 2636 3442 3612
Size: 606 2030 806 170
Fragments arranged by size:
2030 806 606 170
AhdI GACnn_n'nnGTC
Cuts at: 0 1250 3612
Size: 1250 2362
AleI CACnn'nnGTG
Cuts at: 0 2308 3612
Size: 2308 1304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3410 3612
Size: 3410 202
AlwNI CAG_nnn'CTG
Cuts at: 0 2206 3612
Size: 2206 1406
ApaLI G'TGCA_C
Cuts at: 0 164 1157 3612
Size: 164 993 2455
Fragments arranged by size:
2455 993 164
ApoI r'AATT_y
Cuts at: 0 475 1571 1800 2068 2403 2423 3612
Size: 475 1096 229 268 335 20 1189
Fragments arranged by size:
1189 1096 475 335 268 229 20
BamHI G'GATC_C
Cuts at: 0 3514 3537 3612
Size: 3514 23 75
Fragments arranged by size:
3514 75 23
BanI G'GyrC_C
Cuts at: 0 619 3612
Size: 619 2993
BanII G_rGCy'C
Cuts at: 0 195 563 1106 3612
Size: 195 368 543 2506
Fragments arranged by size:
2506 543 368 195
BbsI GAAGACnn'nnnn_
Cuts at: 0 34 482 1558 2005 2954 3439 3612
Size: 34 448 1076 447 949 485 173
Fragments arranged by size:
1076 949 485 448 447 173 34
BclI T'GATC_A
Cuts at: 0 2589 3612
Size: 2589 1023
BfrBI ATG'CAT
Cuts at: 0 2785 3205 3612
Size: 2785 420 407
Fragments arranged by size:
2785 420 407
BglI GCCn_nnn'nGGC
Cuts at: 0 2478 3612
Size: 2478 1134
BlpI GC'TnA_GC
Cuts at: 0 1223 3035 3281 3612
Size: 1223 1812 246 331
Fragments arranged by size:
1812 1223 331 246
Bme1580I G_kGCm'C
Cuts at: 0 168 1161 3612
Size: 168 993 2451
Fragments arranged by size:
2451 993 168
BmgBI CAC'GTC
Cuts at: 0 3445 3612
Size: 3445 167
BmrI ACTGGGnnnn_n'
Cuts at: 0 2287 3612
Size: 2287 1325
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1271 3612
Size: 1271 2341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 1054 3612
Size: 248 806 2558
Fragments arranged by size:
2558 806 248
Bpu10I CC'TnA_GC
Cuts at: 0 194 313 2798 3128 3612
Size: 194 119 2485 330 484
Fragments arranged by size:
2485 484 330 194 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 1560 2072 3172 3612
Size: 769 791 512 1100 440
Fragments arranged by size:
1100 791 769 512 440
BsaI GGTCTCn'nnnn_
Cuts at: 0 982 3327 3485 3612
Size: 982 2345 158 127
Fragments arranged by size:
2345 982 158 127
BsaBI GATnn'nnATC
Cuts at: 0 1189 2062 3612
Size: 1189 873 1550
Fragments arranged by size:
1550 1189 873
BsaHI Gr'CG_yC
Cuts at: 0 1412 3612
Size: 1412 2200
BsaWI w'CCGG_w
Cuts at: 0 938 2754 3612
Size: 938 1816 858
Fragments arranged by size:
1816 938 858
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1740 1770 2120 2150 2736 2766 2904 2934
Size: 1740 30 350 30 586 30 138 30
Cuts at: 2934 3612
Size: 678
Fragments arranged by size:
1740 678 586 350 138 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 344 2866 3612
Size: 344 2522 746
Fragments arranged by size:
2522 746 344
BseYI C'CCAG_C
Cuts at: 0 188 1270 3612
Size: 188 1082 2342
Fragments arranged by size:
2342 1082 188
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2828 3612
Size: 2828 784
BsiEI CG_ry'CG
Cuts at: 0 3086 3612
Size: 3086 526
BsiHKAI G_wGCw'C
Cuts at: 0 168 563 1161 1955 2682 3612
Size: 168 395 598 794 727 930
Fragments arranged by size:
930 794 727 598 395 168
BsmI GAATG_Cn'
Cuts at: 0 1046 2118 2231 3612
Size: 1046 1072 113 1381
Fragments arranged by size:
1381 1072 1046 113
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1419 2140 3612
Size: 1419 721 1472
Fragments arranged by size:
1472 1419 721
Bsp1286I G_dGCh'C
Cuts at: 0 168 195 563 1106 1161 1955 2682 3612
Size: 168 27 368 543 55 794 727 930
Fragments arranged by size:
930 794 727 543 368 168 55 27
BspEI T'CCGG_A
Cuts at: 0 938 2754 3612
Size: 938 1816 858
Fragments arranged by size:
1816 938 858
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 177 1575 3079 3612
Size: 177 1398 1504 533
Fragments arranged by size:
1504 1398 533 177
BsrBI CCG'CTC
Cuts at: 0 2797 3612
Size: 2797 815
BsrDI GCAATG_nn'
Cuts at: 0 80 3014 3029 3612
Size: 80 2934 15 583
Fragments arranged by size:
2934 583 80 15
BsrGI T'GTAC_A
Cuts at: 0 34 1793 3011 3546 3612
Size: 34 1759 1218 535 66
Fragments arranged by size:
1759 1218 535 66 34
BstAPI GCAn_nnn'nTGC
Cuts at: 0 163 1233 3612
Size: 163 1070 2379
Fragments arranged by size:
2379 1070 163
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3476 3612
Size: 3476 136
BstYI r'GATC_y
Cuts at: 0 2565 3021 3514 3537 3612
Size: 2565 456 493 23 75
Fragments arranged by size:
2565 493 456 75 23
BstZ17I GTA'TAC
Cuts at: 0 2543 3196 3612
Size: 2543 653 416
Fragments arranged by size:
2543 653 416
Bsu36I CC'TnA_GG
Cuts at: 0 2581 3612
Size: 2581 1031
BtgI C'CryG_G
Cuts at: 0 266 2416 3612
Size: 266 2150 1196
Fragments arranged by size:
2150 1196 266
BtsI GCAGTG_nn'
Cuts at: 0 342 3612
Size: 342 3270
DraIII CAC_nnn'GTG
Cuts at: 0 163 1710 3223 3612
Size: 163 1547 1513 389
Fragments arranged by size:
1547 1513 389 163
EaeI y'GGCC_r
Cuts at: 0 644 2792 3612
Size: 644 2148 820
Fragments arranged by size:
2148 820 644
EarI CTCTTCn'nnn_
Cuts at: 0 1130 1970 1988 2843 3612
Size: 1130 840 18 855 769
Fragments arranged by size:
1130 855 840 769 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1900 3612
Size: 1900 1712
EcoICRI GAG'CTC
Cuts at: 0 561 3612
Size: 561 3051
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 248 349 614 1054 1232 1391 2491
Size: 28 220 101 265 440 178 159 1100
Cuts at: 2491 2707 2867 3612
Size: 216 160 745
Fragments arranged by size:
1100 745 440 265 220 216 178 160
159 101 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1542 3612
Size: 1542 2070
EcoO109I rG'GnC_Cy
Cuts at: 0 681 780 1611 3612
Size: 681 99 831 2001
Fragments arranged by size:
2001 831 681 99
EcoRI G'AATT_C
Cuts at: 0 2403 2423 3612
Size: 2403 20 1189
Fragments arranged by size:
2403 1189 20
EcoRV GAT'ATC
Cuts at: 0 2650 3612
Size: 2650 962
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 736 1575 1999 2689 3612
Size: 736 839 424 690 923
Fragments arranged by size:
923 839 736 690 424
HaeII r_GCGC'y
Cuts at: 0 2921 3612
Size: 2921 691
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 332 364 834 866 1236 1268 2550 2553
Size: 332 32 470 32 370 32 1282 3
Cuts at: 2553 2582 2585 3349 3381 3612
Size: 29 3 764 32 231
Fragments arranged by size:
1282 764 470 370 332 231 32 32
32 32 29 3 3
HincII GTy'rAC
Cuts at: 0 243 2378 3263 3612
Size: 243 2135 885 349
Fragments arranged by size:
2135 885 349 243
HindIII A'AGCT_T
Cuts at: 0 596 866 3037 3612
Size: 596 270 2171 575
Fragments arranged by size:
2171 596 575 270
HpaI GTT'AAC
Cuts at: 0 2378 3263 3612
Size: 2378 885 349
Fragments arranged by size:
2378 885 349
MfeI C'AATT_G
Cuts at: 0 2726 3612
Size: 2726 886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 1351 2718 3612
Size: 491 860 1367 894
Fragments arranged by size:
1367 894 860 491
MscI TGG'CCA
Cuts at: 0 646 3612
Size: 646 2966
MslI CAynn'nnrTG
Cuts at: 0 802 2160 2308 3612
Size: 802 1358 148 1304
Fragments arranged by size:
1358 1304 802 148
MspA1I CmG'CkG
Cuts at: 0 1270 1350 1740 2287 2812 3612
Size: 1270 80 390 547 525 800
Fragments arranged by size:
1270 800 547 525 390 80
NdeI CA'TA_TG
Cuts at: 0 2364 3612
Size: 2364 1248
NsiI A_TGCA'T
Cuts at: 0 2787 3207 3612
Size: 2787 420 405
Fragments arranged by size:
2787 420 405
NspI r_CATG'y
Cuts at: 0 610 1144 2640 3612
Size: 610 534 1496 972
Fragments arranged by size:
1496 972 610 534
PciI A'CATG_T
Cuts at: 0 606 2636 3612
Size: 606 2030 976
Fragments arranged by size:
2030 976 606
PflMI CCAn_nnn'nTGG
Cuts at: 0 829 3612
Size: 829 2783
PpuMI rG'GwC_Cy
Cuts at: 0 1611 3612
Size: 1611 2001
PsiI TTA'TAA
Cuts at: 0 3506 3612
Size: 3506 106
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1853 1885 3612
Size: 1853 32 1727
Fragments arranged by size:
1853 1727 32
PstI C_TGCA'G
Cuts at: 0 534 1238 2099 3612
Size: 534 704 861 1513
Fragments arranged by size:
1513 861 704 534
PvuII CAG'CTG
Cuts at: 0 1270 1350 1740 2287 2812 3612
Size: 1270 80 390 547 525 800
Fragments arranged by size:
1270 800 547 525 390 80
SacI G_AGCT'C
Cuts at: 0 563 3612
Size: 563 3049
SanDI GG'GwC_CC
Cuts at: 0 1611 3612
Size: 1611 2001
SapI GCTCTTCn'nnn_
Cuts at: 0 1130 1988 3612
Size: 1130 858 1624
Fragments arranged by size:
1624 1130 858
ScaI AGT'ACT
Cuts at: 0 1404 3612
Size: 1404 2208
SfcI C'TryA_G
Cuts at: 0 530 578 1234 2095 3612
Size: 530 48 656 861 1517
Fragments arranged by size:
1517 861 656 530 48
SmlI C'TyrA_G
Cuts at: 0 784 1539 2087 3151 3612
Size: 784 755 548 1064 461
Fragments arranged by size:
1064 784 755 548 461
SspI AAT'ATT
Cuts at: 0 1878 3612
Size: 1878 1734
StuI AGG'CCT
Cuts at: 0 827 2709 3612
Size: 827 1882 903
Fragments arranged by size:
1882 903 827
StyI C'CwwG_G
Cuts at: 0 828 1884 3612
Size: 828 1056 1728
Fragments arranged by size:
1728 1056 828
TatI w'GTAC_w
Cuts at: 0 34 537 1172 1330 1402 1454 1793 3011
Size: 34 503 635 158 72 52 339 1218
Cuts at: 3011 3546 3612
Size: 535 66
Fragments arranged by size:
1218 635 535 503 339 158 72 66
52 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2904 2936 3612
Size: 2904 32 676
Fragments arranged by size:
2904 676 32
Tth111I GACn'n_nGTC
Cuts at: 0 1492 3612
Size: 1492 2120
XbaI T'CTAG_A
Cuts at: 0 3114 3612
Size: 3114 498
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1891 3612
Size: 1891 1721
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AlfI
AlwNI ApaLI ApoI BamHI BanI BanII BbsI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrGI BstAPI BstXI BstYI BstZ17I
Bsu36I BtgI BtsI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I
NdeI NsiI NspI PciI PflMI PpuMI PsiI PsrI
PstI PvuII SacI SanDI SapI ScaI SfcI SmlI
SspI StuI StyI TatI TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AloI ApaI AscI
AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI
BciVI BglII BmtI BsaAI BsiWI BspHI BsrFI BssHII
BssSI BstBI BstEII BtgZI ClaI CspCI DraI DrdI
EagI FseI FspI FspAI KasI KpnI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PasI
PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PvuI
RsrII SacII SalI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TaqII
XhoI XmaI XmnI ZraI