Restriction map for
pF1KSDA1290
(Linear) (Six-base) MAPSORT of: KIAA1290.seq Check: 8592 from: 1 to: 3030
KIAA1290.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2637 3030
Size: 2637 393
AatII G_ACGT'C
Cuts at: 0 71 3030
Size: 71 2959
Acc65I G'GTAC_C
Cuts at: 0 1267 3030
Size: 1267 1763
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 475 1591 1975 2636 2995 3030
Size: 204 271 1116 384 661 359 35
Fragments arranged by size:
1116 661 384 359 271 204 35
AflIII A'CryG_T
Cuts at: 0 2056 3030
Size: 2056 974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1987 2305 3030
Size: 1987 318 725
Fragments arranged by size:
1987 725 318
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 613 645 3030
Size: 613 32 2385
Fragments arranged by size:
2385 613 32
AlwNI CAG_nnn'CTG
Cuts at: 0 357 1405 2496 2718 2932 3030
Size: 357 1048 1091 222 214 98
Fragments arranged by size:
1091 1048 357 222 214 98
ApaI G_GGCC'C
Cuts at: 0 2323 2640 3030
Size: 2323 317 390
Fragments arranged by size:
2323 390 317
ApaLI G'TGCA_C
Cuts at: 0 1273 3030
Size: 1273 1757
AvaI C'yCGr_G
Cuts at: 0 1355 3030
Size: 1355 1675
BamHI G'GATC_C
Cuts at: 0 659 1784 3030
Size: 659 1125 1246
Fragments arranged by size:
1246 1125 659
BanI G'GyrC_C
Cuts at: 0 584 1267 1560 2018 2549 3030
Size: 584 683 293 458 531 481
Fragments arranged by size:
683 584 531 481 458 293
BanII G_rGCy'C
Cuts at: 0 550 991 1517 1757 2323 2640 3030
Size: 550 441 526 240 566 317 390
Fragments arranged by size:
566 550 526 441 390 317 240
BbeI G_GCGC'C
Cuts at: 0 588 3030
Size: 588 2442
BbsI GAAGACnn'nnnn_
Cuts at: 0 761 1126 3030
Size: 761 365 1904
Fragments arranged by size:
1904 761 365
BbvCI CC'TCA_GC
Cuts at: 0 10 2545 3030
Size: 10 2535 485
Fragments arranged by size:
2535 485 10
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2421 2455 3030
Size: 2421 34 575
Fragments arranged by size:
2421 575 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2595 2982 3030
Size: 2595 387 48
Fragments arranged by size:
2595 387 48
BclI T'GATC_A
Cuts at: 0 455 2777 3030
Size: 455 2322 253
Fragments arranged by size:
2322 455 253
BglI GCCn_nnn'nGGC
Cuts at: 0 803 1493 3030
Size: 803 690 1537
Fragments arranged by size:
1537 803 690
BglII A'GATC_T
Cuts at: 0 944 2753 3030
Size: 944 1809 277
Fragments arranged by size:
1809 944 277
BlpI GC'TnA_GC
Cuts at: 0 257 1978 3030
Size: 257 1721 1052
Fragments arranged by size:
1721 1052 257
Bme1580I G_kGCm'C
Cuts at: 0 446 1277 1606 1835 2005 2323 2640 3030
Size: 446 831 329 229 170 318 317 390
Fragments arranged by size:
831 446 390 329 318 317 229 170
BmgBI CAC'GTC
Cuts at: 0 1278 1350 2057 3030
Size: 1278 72 707 973
Fragments arranged by size:
1278 973 707 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 1926 2190 2217 3030
Size: 1926 264 27 813
Fragments arranged by size:
1926 813 264 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2772 3030
Size: 2772 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 610 640 684 820 958 991 1111
Size: 129 481 30 44 136 138 33 120
Cuts at: 1111 1858 2740 2767 2989 3030
Size: 747 882 27 222 41
Fragments arranged by size:
882 747 481 222 138 136 129 120
44 41 33 30 27
Bpu10I CC'TnA_GC
Cuts at: 0 10 1237 2545 2642 3030
Size: 10 1227 1308 97 388
Fragments arranged by size:
1308 1227 388 97 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 814 3030
Size: 288 526 2216
Fragments arranged by size:
2216 526 288
BsaI GGTCTCn'nnnn_
Cuts at: 0 670 1219 3030
Size: 670 549 1811
Fragments arranged by size:
1811 670 549
BsaAI yAC'GTr
Cuts at: 0 1971 2478 3030
Size: 1971 507 552
Fragments arranged by size:
1971 552 507
BsaHI Gr'CG_yC
Cuts at: 0 68 585 1193 3030
Size: 68 517 608 1837
Fragments arranged by size:
1837 608 517 68
BsaWI w'CCGG_w
Cuts at: 0 1049 1649 1904 3030
Size: 1049 600 255 1126
Fragments arranged by size:
1126 1049 600 255
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 148 178 418 448 613 643 973 1003
Size: 148 30 240 30 165 30 330 30
Cuts at: 1003 1234 1264 2182 2212 3030
Size: 231 30 918 30 818
Fragments arranged by size:
918 818 330 240 231 165 148 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 721 1320 1675 2737 2845 3030
Size: 172 549 599 355 1062 108 185
Fragments arranged by size:
1062 599 549 355 185 172 108
BseYI C'CCAG_C
Cuts at: 0 395 1526 1773 2148 2925 3030
Size: 395 1131 247 375 777 105
Fragments arranged by size:
1131 777 395 375 247 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 370 1132 1440 2652 2671 2918 3030
Size: 44 326 762 308 1212 19 247 112
Fragments arranged by size:
1212 762 326 308 247 112 44 19
BsiEI CG_ry'CG
Cuts at: 0 1346 1650 3030
Size: 1346 304 1380
Fragments arranged by size:
1380 1346 304
BsiHKAI G_wGCw'C
Cuts at: 0 1277 2330 2837 3030
Size: 1277 1053 507 193
Fragments arranged by size:
1277 1053 507 193
BsmI GAATG_Cn'
Cuts at: 0 413 1392 1871 2392 3030
Size: 413 979 479 521 638
Fragments arranged by size:
979 638 521 479 413
Bsp1286I G_dGCh'C
Cuts at: 0 446 550 991 1277 1517 1606 1757 1835
Size: 446 104 441 286 240 89 151 78
Cuts at: 1835 2005 2323 2330 2640 2837 3030
Size: 170 318 7 310 197 193
Fragments arranged by size:
446 441 318 310 286 240 197 193
170 151 104 89 78 7
BspHI T'CATG_A
Cuts at: 0 2876 3030
Size: 2876 154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 83 281 1253 1664 2637 3030
Size: 83 198 972 411 973 393
Fragments arranged by size:
973 972 411 393 198 83
BsrBI CCG'CTC
Cuts at: 0 1328 1533 2703 3030
Size: 1328 205 1170 327
Fragments arranged by size:
1328 1170 327 205
BsrDI GCAATG_nn'
Cuts at: 0 2373 3030
Size: 2373 657
BsrFI r'CCGG_y
Cuts at: 0 108 1487 2015 2251 2460 2486 3030
Size: 108 1379 528 236 209 26 544
Fragments arranged by size:
1379 544 528 236 209 108 26
BsrGI T'GTAC_A
Cuts at: 0 1538 3030
Size: 1538 1492
BssHII G'CGCG_C
Cuts at: 0 2892 3030
Size: 2892 138
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1769 2496 3030
Size: 1769 727 534
Fragments arranged by size:
1769 727 534
BstEII G'GTnAC_C
Cuts at: 0 382 3030
Size: 382 2648
BstXI CCAn_nnnn'nTGG
Cuts at: 0 153 327 613 858 2178 3030
Size: 153 174 286 245 1320 852
Fragments arranged by size:
1320 852 286 245 174 153
BstYI r'GATC_y
Cuts at: 0 374 659 944 1547 1652 1784 1850 2492
Size: 374 285 285 603 105 132 66 642
Cuts at: 2492 2753 3030
Size: 261 277
Fragments arranged by size:
642 603 374 285 285 277 261 132
105 66
Bsu36I CC'TnA_GG
Cuts at: 0 1790 2887 3030
Size: 1790 1097 143
Fragments arranged by size:
1790 1097 143
BtgI C'CryG_G
Cuts at: 0 262 1143 1778 2159 2301 3030
Size: 262 881 635 381 142 729
Fragments arranged by size:
881 729 635 381 262 142
BtsI GCAGTG_nn'
Cuts at: 0 655 1103 1428 1821 3030
Size: 655 448 325 393 1209
Fragments arranged by size:
1209 655 448 393 325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 522 557 3030
Size: 522 35 2473
Fragments arranged by size:
2473 522 35
DraIII CAC_nnn'GTG
Cuts at: 0 390 2105 2967 3030
Size: 390 1715 862 63
Fragments arranged by size:
1715 862 390 63
EaeI y'GGCC_r
Cuts at: 0 110 914 1206 1494 1820 2162 2253 2462
Size: 110 804 292 288 326 342 91 209
Cuts at: 2462 2732 2916 3030
Size: 270 184 114
Fragments arranged by size:
804 342 326 292 288 270 209 184
114 110 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 994 3030
Size: 994 2036
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 204 475 610 640 684 820 958
Size: 129 75 271 135 30 44 136 138
Cuts at: 958 991 1111 1591 1858 1975 2636 2740 2767
Size: 33 120 480 267 117 661 104 27
Cuts at: 2767 2989 2995 3030
Size: 222 6 35
Fragments arranged by size:
661 480 271 267 222 138 136 135
129 120 117 104 75 44 35 33
30 27 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 493 508 1954 2188 2695 3030
Size: 493 15 1446 234 507 335
Fragments arranged by size:
1446 507 493 335 234 15
EcoO109I rG'GnC_Cy
Cuts at: 0 95 933 2091 2637 2715 2922 3030
Size: 95 838 1158 546 78 207 108
Fragments arranged by size:
1158 838 546 207 108 95 78
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2586 3030
Size: 2586 444
HaeII r_GCGC'y
Cuts at: 0 588 3030
Size: 588 2442
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 277 309 366 398 418 450 973 1005
Size: 277 32 57 32 20 32 523 32
Cuts at: 1005 1234 1266 2317 2349 2548 2580 3030
Size: 229 32 1051 32 199 32 450
Fragments arranged by size:
1051 523 450 277 229 199 57 32
32 32 32 32 32 32 20
HincII GTy'rAC
Cuts at: 0 1287 2046 2448 3030
Size: 1287 759 402 582
Fragments arranged by size:
1287 759 582 402
KasI G'GCGC_C
Cuts at: 0 584 3030
Size: 584 2446
KpnI G_GTAC'C
Cuts at: 0 1271 3030
Size: 1271 1759
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 289 1694 3030
Size: 158 131 1405 1336
Fragments arranged by size:
1405 1336 158 131
MscI TGG'CCA
Cuts at: 0 916 1208 1496 1822 2164 2734 3030
Size: 916 292 288 326 342 570 296
Fragments arranged by size:
916 570 342 326 296 292 288
MslI CAynn'nnrTG
Cuts at: 0 1385 1969 3030
Size: 1385 584 1061
Fragments arranged by size:
1385 1061 584
MspA1I CmG'CkG
Cuts at: 0 9 399 522 1982 2517 2604 2655 2929
Size: 9 390 123 1460 535 87 51 274
Cuts at: 2929 3030
Size: 101
Fragments arranged by size:
1460 535 390 274 123 101 87 51
9
NaeI GCC'GGC
Cuts at: 0 1489 2488 3030
Size: 1489 999 542
Fragments arranged by size:
1489 999 542
NarI GG'CG_CC
Cuts at: 0 585 3030
Size: 585 2445
NcoI C'CATG_G
Cuts at: 0 2159 2301 3030
Size: 2159 142 729
Fragments arranged by size:
2159 729 142
NdeI CA'TA_TG
Cuts at: 0 2905 3030
Size: 2905 125
NgoMIV G'CCGG_C
Cuts at: 0 1487 2486 3030
Size: 1487 999 544
Fragments arranged by size:
1487 999 544
NspI r_CATG'y
Cuts at: 0 1020 1772 1800 3030
Size: 1020 752 28 1230
Fragments arranged by size:
1230 1020 752 28
PasI CC'CwG_GG
Cuts at: 0 408 1155 3030
Size: 408 747 1875
Fragments arranged by size:
1875 747 408
PflMI CCAn_nnn'nTGG
Cuts at: 0 1215 2307 2718 3030
Size: 1215 1092 411 312
Fragments arranged by size:
1215 1092 411 312
PfoI T'CCnGG_A
Cuts at: 0 2587 3030
Size: 2587 443
PmlI CAC'GTG
Cuts at: 0 1971 2478 3030
Size: 1971 507 552
Fragments arranged by size:
1971 552 507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 613 645 1504 1536 3030
Size: 613 32 859 32 1494
Fragments arranged by size:
1494 859 613 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 95 933 2715 2922 3030
Size: 95 838 1782 207 108
Fragments arranged by size:
1782 838 207 108 95
PspOMI G'GGCC_C
Cuts at: 0 2319 2636 3030
Size: 2319 317 394
Fragments arranged by size:
2319 394 317
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2833 2865 3030
Size: 2833 32 165
Fragments arranged by size:
2833 165 32
PstI C_TGCA'G
Cuts at: 0 300 1619 2360 3030
Size: 300 1319 741 670
Fragments arranged by size:
1319 741 670 300
PvuII CAG'CTG
Cuts at: 0 9 399 522 3030
Size: 9 390 123 2508
Fragments arranged by size:
2508 390 123 9
SanDI GG'GwC_CC
Cuts at: 0 2922 3030
Size: 2922 108
SbfI CC_TGCA'GG
Cuts at: 0 1619 3030
Size: 1619 1411
SexAI A'CCwGG_T
Cuts at: 0 1472 2690 2963 3030
Size: 1472 1218 273 67
Fragments arranged by size:
1472 1218 273 67
SfcI C'TryA_G
Cuts at: 0 296 1615 2356 3030
Size: 296 1319 741 674
Fragments arranged by size:
1319 741 674 296
SfoI GGC'GCC
Cuts at: 0 586 3030
Size: 586 2444
SgrAI Cr'CCGG_yG
Cuts at: 0 1487 3030
Size: 1487 1543
SmaI CCC'GGG
Cuts at: 0 1357 3030
Size: 1357 1673
SmlI C'TyrA_G
Cuts at: 0 303 829 3030
Size: 303 526 2201
Fragments arranged by size:
2201 526 303
StuI AGG'CCT
Cuts at: 0 3010 3030
Size: 3010 20
StyI C'CwwG_G
Cuts at: 0 1679 2159 2301 2399 3030
Size: 1679 480 142 98 631
Fragments arranged by size:
1679 631 480 142 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1360 1540 1819 2256 2567 3030
Size: 1360 180 279 437 311 463
Fragments arranged by size:
1360 463 437 311 279 180
TatI w'GTAC_w
Cuts at: 0 164 1538 1931 2681 3030
Size: 164 1374 393 750 349
Fragments arranged by size:
1374 750 393 349 164
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 53 85 3030
Size: 53 32 2945
Fragments arranged by size:
2945 53 32
Tth111I GACn'n_nGTC
Cuts at: 0 1474 3030
Size: 1474 1556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 327 471 2742 3030
Size: 327 144 2271 288
Fragments arranged by size:
2271 327 288 144
XmaI C'CCGG_G
Cuts at: 0 1355 3030
Size: 1355 1675
ZraI GAC'GTC
Cuts at: 0 69 3030
Size: 69 2961
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AlfI AloI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI DraIII EaeI EciI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PflMI PfoI PmlI PpiI PpuMI PspOMI PsrI PstI
PvuII SanDI SbfI SexAI SfcI SfoI SgrAI SmaI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AleI ApoI
AscI AseI AsiSI AvrII BaeI BfrBI BmtI BsaBI
BsiWI BsmBI BspEI BssSI BstBI BstZ17I BtgZI ClaI
DraI DrdI EagI EarI EcoICRI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI MfeI MluI NheI NotI
NruI NsiI PacI PciI PmeI PshAI PsiI PspXI
PvuI RsrII SacI SacII SalI SapI ScaI SfiI
SgfI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI XmnI