Restriction map for
pF1KA1292
(Linear) (Six-base) MAPSORT of: KIAA1292.seq Check: 8317 from: 1 to: 2106
KIAA1292.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 459 873 1821 2106
Size: 459 414 948 285
Fragments arranged by size:
948 459 414 285
AatII G_ACGT'C
Cuts at: 0 1690 1735 2106
Size: 1690 45 371
Fragments arranged by size:
1690 371 45
AccI GT'mk_AC
Cuts at: 0 317 2106
Size: 317 1789
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 1368 1972 2106
Size: 700 668 604 134
Fragments arranged by size:
700 668 604 134
AhdI GACnn_n'nnGTC
Cuts at: 0 993 1440 2106
Size: 993 447 666
Fragments arranged by size:
993 666 447
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 814 2106
Size: 814 1292
AlwNI CAG_nnn'CTG
Cuts at: 0 176 946 1161 1827 2106
Size: 176 770 215 666 279
Fragments arranged by size:
770 666 279 215 176
ApaI G_GGCC'C
Cuts at: 0 1181 1402 1600 1652 1784 1873 2030 2106
Size: 1181 221 198 52 132 89 157 76
Fragments arranged by size:
1181 221 198 157 132 89 76 52
ApaLI G'TGCA_C
Cuts at: 0 281 1721 2106
Size: 281 1440 385
Fragments arranged by size:
1440 385 281
AvaI C'yCGr_G
Cuts at: 0 32 446 509 1089 1122 1409 1601 1776
Size: 32 414 63 580 33 287 192 175
Cuts at: 1776 1986 2106
Size: 210 120
Fragments arranged by size:
580 414 287 210 192 175 120 63
33 32
BanI G'GyrC_C
Cuts at: 0 555 909 1663 2106
Size: 555 354 754 443
Fragments arranged by size:
754 555 443 354
BanII G_rGCy'C
Cuts at: 0 75 1181 1402 1418 1600 1652 1784 1873
Size: 75 1106 221 16 182 52 132 89
Cuts at: 1873 2030 2106
Size: 157 76
Fragments arranged by size:
1106 221 182 157 132 89 76 75
52 16
BbeI G_GCGC'C
Cuts at: 0 559 1667 2106
Size: 559 1108 439
Fragments arranged by size:
1108 559 439
BbsI GAAGACnn'nnnn_
Cuts at: 0 1960 2106
Size: 1960 146
BbvCI CC'TCA_GC
Cuts at: 0 335 1946 2106
Size: 335 1611 160
Fragments arranged by size:
1611 335 160
BciVI GTATCCnnnnn_n'
Cuts at: 0 115 1312 2106
Size: 115 1197 794
Fragments arranged by size:
1197 794 115
BclI T'GATC_A
Cuts at: 0 1755 2106
Size: 1755 351
BglI GCCn_nnn'nGGC
Cuts at: 0 422 582 1133 2106
Size: 422 160 551 973
Fragments arranged by size:
973 551 422 160
BglII A'GATC_T
Cuts at: 0 2096 2106
Size: 2096 10
BlpI GC'TnA_GC
Cuts at: 0 1069 2106
Size: 1069 1037
Bme1580I G_kGCm'C
Cuts at: 0 285 834 914 1181 1402 1600 1652 1725
Size: 285 549 80 267 221 198 52 73
Cuts at: 1725 1784 1873 2030 2106
Size: 59 89 157 76
Fragments arranged by size:
549 285 267 221 198 157 89 80
76 73 59 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 485 2106
Size: 485 1621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1611 2106
Size: 1611 495
Bpu10I CC'TnA_GC
Cuts at: 0 335 1108 1946 2106
Size: 335 773 838 160
Fragments arranged by size:
838 773 335 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1099 2106
Size: 637 462 1007
Fragments arranged by size:
1007 637 462
BsaAI yAC'GTr
Cuts at: 0 134 321 537 567 2106
Size: 134 187 216 30 1539
Fragments arranged by size:
1539 216 187 134 30
BsaHI Gr'CG_yC
Cuts at: 0 237 296 556 1541 1664 1687 1732 2106
Size: 237 59 260 985 123 23 45 374
Fragments arranged by size:
985 374 260 237 123 59 45 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1501 1731 2106
Size: 1501 230 375
Fragments arranged by size:
1501 375 230
BseYI C'CCAG_C
Cuts at: 0 342 1299 1395 1555 1645 1789 2001 2106
Size: 342 957 96 160 90 144 212 105
Fragments arranged by size:
957 342 212 160 144 105 96 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 862 1799 1951 2106
Size: 629 233 937 152 155
Fragments arranged by size:
937 629 233 155 152
BsiHKAI G_wGCw'C
Cuts at: 0 285 536 1725 2106
Size: 285 251 1189 381
Fragments arranged by size:
1189 381 285 251
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1625 2106
Size: 1625 481
Bsp1286I G_dGCh'C
Cuts at: 0 75 285 536 834 914 1181 1402 1418
Size: 75 210 251 298 80 267 221 16
Cuts at: 1418 1600 1652 1725 1784 1873 2030 2106
Size: 182 52 73 59 89 157 76
Fragments arranged by size:
298 267 251 221 210 182 157 89
80 76 75 73 59 52 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 459 806 873 924 1343 1821 2016 2106
Size: 459 347 67 51 419 478 195 90
Fragments arranged by size:
478 459 419 347 195 90 67 51
BsrBI CCG'CTC
Cuts at: 0 37 591 651 1068 2106
Size: 37 554 60 417 1038
Fragments arranged by size:
1038 554 417 60 37
BsrFI r'CCGG_y
Cuts at: 0 388 722 885 1127 2014 2106
Size: 388 334 163 242 887 92
Fragments arranged by size:
887 388 334 242 163 92
BsrGI T'GTAC_A
Cuts at: 0 80 872 2106
Size: 80 792 1234
Fragments arranged by size:
1234 792 80
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1161 2106
Size: 1161 945
BstEII G'GTnAC_C
Cuts at: 0 1808 2106
Size: 1808 298
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 369 2106
Size: 12 357 1737
Fragments arranged by size:
1737 357 12
BstYI r'GATC_y
Cuts at: 0 2096 2106
Size: 2096 10
Bsu36I CC'TnA_GG
Cuts at: 0 619 2106
Size: 619 1487
BtgI C'CryG_G
Cuts at: 0 368 486 896 1363 1726 2106
Size: 368 118 410 467 363 380
Fragments arranged by size:
467 410 380 368 363 118
BtsI GCAGTG_nn'
Cuts at: 0 165 207 757 798 825 1216 1800 2106
Size: 165 42 550 41 27 391 584 306
Fragments arranged by size:
584 550 391 306 165 42 41 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 728 763 2106
Size: 728 35 1343
Fragments arranged by size:
1343 728 35
DraIII CAC_nnn'GTG
Cuts at: 0 280 291 2106
Size: 280 11 1815
Fragments arranged by size:
1815 280 11
DrdI GACnn_nn'nnGTC
Cuts at: 0 1540 1692 2106
Size: 1540 152 414
Fragments arranged by size:
1540 414 152
EaeI y'GGCC_r
Cuts at: 0 2 386 1278 1313 2106
Size: 2 384 892 35 793
Fragments arranged by size:
892 793 384 35 2
EarI CTCTTCn'nnn_
Cuts at: 0 400 1531 2106
Size: 400 1131 575
Fragments arranged by size:
1131 575 400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 1368 1611 1972 2106
Size: 700 668 243 361 134
Fragments arranged by size:
700 668 361 243 134
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1573 2106
Size: 1573 533
EcoO109I rG'GnC_Cy
Cuts at: 0 901 943 1035 1177 1178 1399 1596 1597
Size: 901 42 92 142 1 221 197 1
Cuts at: 1597 1606 1780 1781 1824 1870 2005 2026 2027
Size: 9 174 1 43 46 135 21 1
Cuts at: 2027 2106
Size: 79
Fragments arranged by size:
901 221 197 174 142 135 92 79
46 43 42 21 9 1 1 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 930 2106
Size: 930 1176
FseI GG_CCGG'CC
Cuts at: 0 392 2106
Size: 392 1714
HaeII r_GCGC'y
Cuts at: 0 559 1076 1097 1500 1667 2106
Size: 559 517 21 403 167 439
Fragments arranged by size:
559 517 439 403 167 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 979 1011 1072 1104 1426 1458 2106
Size: 979 32 61 32 322 32 648
Fragments arranged by size:
979 648 322 61 32 32 32
HincII GTy'rAC
Cuts at: 0 222 2106
Size: 222 1884
HindIII A'AGCT_T
Cuts at: 0 661 1969 2106
Size: 661 1308 137
Fragments arranged by size:
1308 661 137
KasI G'GCGC_C
Cuts at: 0 555 1663 2106
Size: 555 1108 443
Fragments arranged by size:
1108 555 443
MscI TGG'CCA
Cuts at: 0 4 1280 2106
Size: 4 1276 826
Fragments arranged by size:
1276 826 4
MslI CAynn'nnrTG
Cuts at: 0 10 367 382 2106
Size: 10 357 15 1724
Fragments arranged by size:
1724 357 15 10
MspA1I CmG'CkG
Cuts at: 0 78 488 1728 1950 2106
Size: 78 410 1240 222 156
Fragments arranged by size:
1240 410 222 156 78
NaeI GCC'GGC
Cuts at: 0 390 887 1129 2106
Size: 390 497 242 977
Fragments arranged by size:
977 497 390 242
NarI GG'CG_CC
Cuts at: 0 556 1664 2106
Size: 556 1108 442
Fragments arranged by size:
1108 556 442
NcoI C'CATG_G
Cuts at: 0 368 2106
Size: 368 1738
NgoMIV G'CCGG_C
Cuts at: 0 388 885 1127 2106
Size: 388 497 242 979
Fragments arranged by size:
979 497 388 242
NspI r_CATG'y
Cuts at: 0 1045 2106
Size: 1045 1061
PasI CC'CwG_GG
Cuts at: 0 215 960 2023 2106
Size: 215 745 1063 83
Fragments arranged by size:
1063 745 215 83
PflMI CCAn_nnn'nTGG
Cuts at: 0 1306 2106
Size: 1306 800
PfoI T'CCnGG_A
Cuts at: 0 632 1444 1466 2106
Size: 632 812 22 640
Fragments arranged by size:
812 640 632 22
PmlI CAC'GTG
Cuts at: 0 134 537 2106
Size: 134 403 1569
Fragments arranged by size:
1569 403 134
PpuMI rG'GwC_Cy
Cuts at: 0 901 1606 2005 2106
Size: 901 705 399 101
Fragments arranged by size:
901 705 399 101
PspOMI G'GGCC_C
Cuts at: 0 1177 1398 1596 1648 1780 1869 2026 2106
Size: 1177 221 198 52 132 89 157 80
Fragments arranged by size:
1177 221 198 157 132 89 80 52
PspXI vC'TCGA_Gb
Cuts at: 0 1122 2106
Size: 1122 984
PstI C_TGCA'G
Cuts at: 0 172 803 1157 1574 1685 1751 2106
Size: 172 631 354 417 111 66 355
Fragments arranged by size:
631 417 355 354 172 111 66
PvuII CAG'CTG
Cuts at: 0 1950 2106
Size: 1950 156
SacII CC_GC'GG
Cuts at: 0 489 1729 2106
Size: 489 1240 377
Fragments arranged by size:
1240 489 377
SanDI GG'GwC_CC
Cuts at: 0 901 2106
Size: 901 1205
SbfI CC_TGCA'GG
Cuts at: 0 1751 2106
Size: 1751 355
SfcI C'TryA_G
Cuts at: 0 168 799 1153 1563 1570 1681 1747 2106
Size: 168 631 354 410 7 111 66 359
Fragments arranged by size:
631 410 359 354 168 111 66 7
SfoI GGC'GCC
Cuts at: 0 557 1665 2106
Size: 557 1108 441
Fragments arranged by size:
1108 557 441
SmaI CCC'GGG
Cuts at: 0 1411 1778 1988 2106
Size: 1411 367 210 118
Fragments arranged by size:
1411 367 210 118
SmlI C'TyrA_G
Cuts at: 0 652 1114 1122 2106
Size: 652 462 8 984
Fragments arranged by size:
984 652 462 8
SphI G_CATG'C
Cuts at: 0 1045 2106
Size: 1045 1061
StuI AGG'CCT
Cuts at: 0 624 1120 2106
Size: 624 496 986
Fragments arranged by size:
986 624 496
StyI C'CwwG_G
Cuts at: 0 368 707 905 2106
Size: 368 339 198 1201
Fragments arranged by size:
1201 368 339 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 653 1313 2068 2106
Size: 653 660 755 38
Fragments arranged by size:
755 660 653 38
TatI w'GTAC_w
Cuts at: 0 80 872 2106
Size: 80 792 1234
Fragments arranged by size:
1234 792 80
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1843 1875 2106
Size: 1843 32 231
Fragments arranged by size:
1843 231 32
Tth111I GACn'n_nGTC
Cuts at: 0 1734 2106
Size: 1734 372
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 366 714 1303 1452 2106
Size: 366 348 589 149 654
Fragments arranged by size:
654 589 366 348 149
XhoI C'TCGA_G
Cuts at: 0 1122 2106
Size: 1122 984
XmaI C'CCGG_G
Cuts at: 0 1409 1776 1986 2106
Size: 1409 367 210 120
Fragments arranged by size:
1409 367 210 120
XmnI GAAnn'nnTTC
Cuts at: 0 502 2106
Size: 502 1604
ZraI GAC'GTC
Cuts at: 0 1688 1733 2106
Size: 1688 45 373
Fragments arranged by size:
1688 373 45
Enzymes that do cut:
AarI AatII AccI AcuI AhdI AlfI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaAI BsaHI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BsrGI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI
EarI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I
HincII HindIII KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI
PspOMI PspXI PstI PvuII SacII SanDI SbfI SfcI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AleI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BfrBI BmgBI BmtI BplI BsaI BsaBI BsaWI BsaXI
BsiEI BsiWI BsmI BspEI BspHI BsrDI BssHII BssSI
BstBI BstZ17I BtgZI ClaI DraI EagI EciI EcoICRI
EcoRI EcoRV FspI FspAI HpaI KpnI MfeI MluI
MmeI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PpiI PshAI PsiI PsrI PvuI RsrII SacI
SalI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI XbaI