Restriction map for
pF1KA1301
(Linear) (Six-base) MAPSORT of: KIAA1301.seq Check: 8519 from: 1 to: 4716
KIAA1301.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 32 2523 4716
Size: 32 2491 2193
Fragments arranged by size:
2491 2193 32
AatII G_ACGT'C
Cuts at: 0 1236 4716
Size: 1236 3480
AccI GT'mk_AC
Cuts at: 0 697 3069 3899 4716
Size: 697 2372 830 817
Fragments arranged by size:
2372 830 817 697
Acc65I G'GTAC_C
Cuts at: 0 941 2402 3529 4716
Size: 941 1461 1127 1187
Fragments arranged by size:
1461 1187 1127 941
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 2363 3043 3380 4716
Size: 509 1854 680 337 1336
Fragments arranged by size:
1854 1336 680 509 337
AflII C'TTAA_G
Cuts at: 0 625 4716
Size: 625 4091
AflIII A'CryG_T
Cuts at: 0 2840 2842 2933 4615 4716
Size: 2840 2 91 1682 101
Fragments arranged by size:
2840 1682 101 91 2
AleI CACnn'nnGTG
Cuts at: 0 935 4716
Size: 935 3781
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3152 3184 4716
Size: 3152 32 1532
Fragments arranged by size:
3152 1532 32
AlwNI CAG_nnn'CTG
Cuts at: 0 861 1000 1117 1931 2245 2364 2538 3479
Size: 861 139 117 814 314 119 174 941
Cuts at: 3479 4068 4716
Size: 589 648
Fragments arranged by size:
941 861 814 648 589 314 174 139
119 117
ApaI G_GGCC'C
Cuts at: 0 386 4716
Size: 386 4330
ApaLI G'TGCA_C
Cuts at: 0 2103 4716
Size: 2103 2613
ApoI r'AATT_y
Cuts at: 0 297 796 3287 4013 4716
Size: 297 499 2491 726 703
Fragments arranged by size:
2491 726 703 499 297
AvaI C'yCGr_G
Cuts at: 0 14 3548 4716
Size: 14 3534 1168
Fragments arranged by size:
3534 1168 14
BamHI G'GATC_C
Cuts at: 0 3935 4716
Size: 3935 781
BanI G'GyrC_C
Cuts at: 0 731 941 1324 2133 2293 2402 3529 4174
Size: 731 210 383 809 160 109 1127 645
Cuts at: 4174 4716
Size: 542
Fragments arranged by size:
1127 809 731 645 542 383 210 160
109
BanII G_rGCy'C
Cuts at: 0 73 106 386 447 1618 1813 2063 2257
Size: 73 33 280 61 1171 195 250 194
Cuts at: 2257 3555 4716
Size: 1298 1161
Fragments arranged by size:
1298 1171 1161 280 250 195 194 73
61 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 962 1199 2045 4716
Size: 962 237 846 2671
Fragments arranged by size:
2671 962 846 237
BciVI GTATCCnnnnn_n'
Cuts at: 0 2605 3816 4402 4716
Size: 2605 1211 586 314
Fragments arranged by size:
2605 1211 586 314
BclI T'GATC_A
Cuts at: 0 885 1463 2858 2985 4716
Size: 885 578 1395 127 1731
Fragments arranged by size:
1731 1395 885 578 127
BfrBI ATG'CAT
Cuts at: 0 1759 4716
Size: 1759 2957
BglII A'GATC_T
Cuts at: 0 570 2545 4716
Size: 570 1975 2171
Fragments arranged by size:
2171 1975 570
BlpI GC'TnA_GC
Cuts at: 0 895 3415 4716
Size: 895 2520 1301
Fragments arranged by size:
2520 1301 895
Bme1580I G_kGCm'C
Cuts at: 0 386 675 2107 2138 4716
Size: 386 289 1432 31 2578
Fragments arranged by size:
2578 1432 386 289 31
BmgBI CAC'GTC
Cuts at: 0 2501 4716
Size: 2501 2215
BmrI ACTGGGnnnn_n'
Cuts at: 0 257 1667 1841 2132 2445 4180 4716
Size: 257 1410 174 291 313 1735 536
Fragments arranged by size:
1735 1410 536 313 291 257 174
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 189 1569 4716
Size: 189 1380 3147
Fragments arranged by size:
3147 1380 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 159 287 886 931 977 1269 1543
Size: 67 92 128 599 45 46 292 274
Cuts at: 1543 2248 2354 2709 3353 3539 3693 4716
Size: 705 106 355 644 186 154 1023
Fragments arranged by size:
1023 705 644 599 355 292 274 186
154 128 106 92 67 46 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1694 2008 2327 4716
Size: 1694 314 319 2389
Fragments arranged by size:
2389 1694 319 314
BsaI GGTCTCn'nnnn_
Cuts at: 0 1783 4716
Size: 1783 2933
BsaAI yAC'GTr
Cuts at: 0 933 2475 2484 2843 3493 4716
Size: 933 1542 9 359 650 1223
Fragments arranged by size:
1542 1223 933 650 359 9
BsaBI GATnn'nnATC
Cuts at: 0 409 4716
Size: 409 4307
BsaHI Gr'CG_yC
Cuts at: 0 39 1233 4716
Size: 39 1194 3483
Fragments arranged by size:
3483 1194 39
BsaWI w'CCGG_w
Cuts at: 0 399 2870 4716
Size: 399 2471 1846
Fragments arranged by size:
2471 1846 399
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 679 709 952 982 1206 1236 2707 2737
Size: 679 30 243 30 224 30 1471 30
Cuts at: 2737 3004 3034 4716
Size: 267 30 1682
Fragments arranged by size:
1682 1471 679 267 243 224 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 1356 1462 1465 1971 2047 2206 2335
Size: 265 1091 106 3 506 76 159 129
Cuts at: 2335 3503 4418 4716
Size: 1168 915 298
Fragments arranged by size:
1168 1091 915 506 298 265 159 129
106 76 3
BseYI C'CCAG_C
Cuts at: 0 207 483 921 1632 2013 2196 2278 2523
Size: 207 276 438 711 381 183 82 245
Cuts at: 2523 3472 3533 4716
Size: 949 61 1183
Fragments arranged by size:
1183 949 711 438 381 276 245 207
183 82 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2072 2705 4716
Size: 2072 633 2011
Fragments arranged by size:
2072 2011 633
BsiEI CG_ry'CG
Cuts at: 0 2512 4716
Size: 2512 2204
BsiHKAI G_wGCw'C
Cuts at: 0 23 106 1102 1618 1981 2107 4716
Size: 23 83 996 516 363 126 2609
Fragments arranged by size:
2609 996 516 363 126 83 23
BsmI GAATG_Cn'
Cuts at: 0 537 1149 1921 2802 3001 4514 4716
Size: 537 612 772 881 199 1513 202
Fragments arranged by size:
1513 881 772 612 537 202 199
BsmBI CGTCTCn'nnnn_
Cuts at: 0 684 4716
Size: 684 4032
Bsp1286I G_dGCh'C
Cuts at: 0 23 73 106 386 447 675 1102 1618
Size: 23 50 33 280 61 228 427 516
Cuts at: 1618 1813 1981 2063 2107 2138 2257 3555 4716
Size: 195 168 82 44 31 119 1298 1161
Fragments arranged by size:
1298 1161 516 427 280 228 195 168
119 82 61 50 44 33 31 23
BspEI T'CCGG_A
Cuts at: 0 2870 4716
Size: 2870 1846
BspHI T'CATG_A
Cuts at: 0 978 4716
Size: 978 3738
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 32 542 1312 1632 2523 3728 4716
Size: 32 510 770 320 891 1205 988
Fragments arranged by size:
1205 988 891 770 510 320 32
BsrBI CCG'CTC
Cuts at: 0 173 4716
Size: 173 4543
BsrDI GCAATG_nn'
Cuts at: 0 1941 4716
Size: 1941 2775
BsrFI r'CCGG_y
Cuts at: 0 2399 2579 4716
Size: 2399 180 2137
Fragments arranged by size:
2399 2137 180
BsrGI T'GTAC_A
Cuts at: 0 4254 4716
Size: 4254 462
BssSI C'ACGA_G
Cuts at: 0 1408 4716
Size: 1408 3308
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2245 2538 4716
Size: 2245 293 2178
Fragments arranged by size:
2245 2178 293
BstBI TT'CG_AA
Cuts at: 0 3578 4716
Size: 3578 1138
BstEII G'GTnAC_C
Cuts at: 0 1429 4716
Size: 1429 3287
BstXI CCAn_nnnn'nTGG
Cuts at: 0 937 2435 4716
Size: 937 1498 2281
Fragments arranged by size:
2281 1498 937
BstYI r'GATC_y
Cuts at: 0 570 2465 2545 2728 3341 3935 4632 4716
Size: 570 1895 80 183 613 594 697 84
Fragments arranged by size:
1895 697 613 594 570 183 84 80
Bsu36I CC'TnA_GG
Cuts at: 0 1895 2624 4716
Size: 1895 729 2092
Fragments arranged by size:
2092 1895 729
BtgI C'CryG_G
Cuts at: 0 429 678 1319 2457 2962 4716
Size: 429 249 641 1138 505 1754
Fragments arranged by size:
1754 1138 641 505 429 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3407 4332 4716
Size: 3407 925 384
Fragments arranged by size:
3407 925 384
BtsI GCAGTG_nn'
Cuts at: 0 1695 2076 2105 2280 2362 4078 4457 4592
Size: 1695 381 29 175 82 1716 379 135
Cuts at: 4592 4716
Size: 124
Fragments arranged by size:
1716 1695 381 379 175 135 124 82
29
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3021 3056 4716
Size: 3021 35 1660
Fragments arranged by size:
3021 1660 35
DraI TTT'AAA
Cuts at: 0 420 954 4716
Size: 420 534 3762
Fragments arranged by size:
3762 534 420
DraIII CAC_nnn'GTG
Cuts at: 0 1976 2501 4716
Size: 1976 525 2215
Fragments arranged by size:
2215 1976 525
EaeI y'GGCC_r
Cuts at: 0 1278 2171 2352 4716
Size: 1278 893 181 2364
Fragments arranged by size:
2364 1278 893 181
EarI CTCTTCn'nnn_
Cuts at: 0 646 1770 3425 3657 4010 4046 4604 4716
Size: 646 1124 1655 232 353 36 558 112
Fragments arranged by size:
1655 1124 646 558 353 232 112 36
EcoICRI GAG'CTC
Cuts at: 0 104 1616 4716
Size: 104 1512 3100
Fragments arranged by size:
3100 1512 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 159 287 509 886 931 977 1269
Size: 67 92 128 222 377 45 46 292
Cuts at: 1269 1543 2248 2354 2363 2709 3043 3353 3380
Size: 274 705 106 9 346 334 310 27
Cuts at: 3380 3539 3693 4716
Size: 159 154 1023
Fragments arranged by size:
1023 705 377 346 334 310 292 274
222 159 154 128 106 92 67 46
45 27 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1226 2081 4716
Size: 1226 855 2635
Fragments arranged by size:
2635 1226 855
EcoO109I rG'GnC_Cy
Cuts at: 0 383 1214 4716
Size: 383 831 3502
Fragments arranged by size:
3502 831 383
EcoRI G'AATT_C
Cuts at: 0 297 4013 4716
Size: 297 3716 703
Fragments arranged by size:
3716 703 297
EcoRV GAT'ATC
Cuts at: 0 2587 4092 4716
Size: 2587 1505 624
Fragments arranged by size:
2587 1505 624
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3021 4716
Size: 3021 1695
HaeII r_GCGC'y
Cuts at: 0 2330 4716
Size: 2330 2386
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 679 711 863 895 952 984 1206 1238
Size: 679 32 152 32 57 32 222 32
Cuts at: 1238 1448 1480 1780 1782 1795 1812 1814 1827
Size: 210 32 300 2 13 17 2 13
Cuts at: 1827 2720 2752 3004 3036 3424 3456 3927 3959
Size: 893 32 252 32 388 32 471 32
Cuts at: 3959 4716
Size: 757
Fragments arranged by size:
893 757 679 471 388 300 252 222
210 152 57 32 32 32 32 32
32 32 32 32 17 13 13 2
2
HincII GTy'rAC
Cuts at: 0 3271 3304 4679 4716
Size: 3271 33 1375 37
Fragments arranged by size:
3271 1375 37 33
KpnI G_GTAC'C
Cuts at: 0 945 2406 3533 4716
Size: 945 1461 1127 1183
Fragments arranged by size:
1461 1183 1127 945
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2718 3950 4716
Size: 2718 1232 766
Fragments arranged by size:
2718 1232 766
MscI TGG'CCA
Cuts at: 0 1280 4716
Size: 1280 3436
MslI CAynn'nnrTG
Cuts at: 0 290 472 935 983 4716
Size: 290 182 463 48 3733
Fragments arranged by size:
3733 463 290 182 48
MspA1I CmG'CkG
Cuts at: 0 132 487 925 1956 2214 2242 2574 2964
Size: 132 355 438 1031 258 28 332 390
Cuts at: 2964 4360 4716
Size: 1396 356
Fragments arranged by size:
1396 1031 438 390 356 355 332 258
132 28
NcoI C'CATG_G
Cuts at: 0 1319 4716
Size: 1319 3397
NdeI CA'TA_TG
Cuts at: 0 3444 4716
Size: 3444 1272
NruI TCG'CGA
Cuts at: 0 2941 4716
Size: 2941 1775
NsiI A_TGCA'T
Cuts at: 0 1761 4716
Size: 1761 2955
NspI r_CATG'y
Cuts at: 0 195 1071 2829 2937 3157 3460 4619 4716
Size: 195 876 1758 108 220 303 1159 97
Fragments arranged by size:
1758 1159 876 303 220 195 108 97
PasI CC'CwG_GG
Cuts at: 0 3637 4716
Size: 3637 1079
PciI A'CATG_T
Cuts at: 0 2933 4615 4716
Size: 2933 1682 101
Fragments arranged by size:
2933 1682 101
PflMI CCAn_nnn'nTGG
Cuts at: 0 269 314 2412 2501 3225 3832 4716
Size: 269 45 2098 89 724 607 884
Fragments arranged by size:
2098 884 724 607 269 89 45
PfoI T'CCnGG_A
Cuts at: 0 2731 3369 4716
Size: 2731 638 1347
Fragments arranged by size:
2731 1347 638
PmlI CAC'GTG
Cuts at: 0 933 2843 3493 4716
Size: 933 1910 650 1223
Fragments arranged by size:
1910 1223 933 650
PpuMI rG'GwC_Cy
Cuts at: 0 1214 4716
Size: 1214 3502
PsiI TTA'TAA
Cuts at: 0 3998 4716
Size: 3998 718
PspOMI G'GGCC_C
Cuts at: 0 382 4716
Size: 382 4334
PstI C_TGCA'G
Cuts at: 0 131 1629 2241 2543 3725 4073 4452 4716
Size: 131 1498 612 302 1182 348 379 264
Fragments arranged by size:
1498 1182 612 379 348 302 264 131
PvuII CAG'CTG
Cuts at: 0 487 925 1956 2242 2574 4360 4716
Size: 487 438 1031 286 332 1786 356
Fragments arranged by size:
1786 1031 487 438 356 332 286
SacI G_AGCT'C
Cuts at: 0 106 1618 4716
Size: 106 1512 3098
Fragments arranged by size:
3098 1512 106
SacII CC_GC'GG
Cuts at: 0 2965 4716
Size: 2965 1751
SapI GCTCTTCn'nnn_
Cuts at: 0 646 1770 4010 4604 4716
Size: 646 1124 2240 594 112
Fragments arranged by size:
2240 1124 646 594 112
SbfI CC_TGCA'GG
Cuts at: 0 1629 4716
Size: 1629 3087
ScaI AGT'ACT
Cuts at: 0 4701 4716
Size: 4701 15
SexAI A'CCwGG_T
Cuts at: 0 152 4716
Size: 152 4564
SfcI C'TryA_G
Cuts at: 0 127 1625 2237 2539 3292 3721 4069 4448
Size: 127 1498 612 302 753 429 348 379
Cuts at: 4448 4716
Size: 268
Fragments arranged by size:
1498 753 612 429 379 348 302 268
127
SmaI CCC'GGG
Cuts at: 0 16 3550 4716
Size: 16 3534 1166
Fragments arranged by size:
3534 1166 16
SmlI C'TyrA_G
Cuts at: 0 625 1709 1987 2342 4711 4716
Size: 625 1084 278 355 2369 5
Fragments arranged by size:
2369 1084 625 355 278 5
SrfI GCCC'GGGC
Cuts at: 0 3550 4716
Size: 3550 1166
SspI AAT'ATT
Cuts at: 0 424 745 3847 4716
Size: 424 321 3102 869
Fragments arranged by size:
3102 869 424 321
StuI AGG'CCT
Cuts at: 0 1460 1893 2622 4716
Size: 1460 433 729 2094
Fragments arranged by size:
2094 1460 729 433
StyI C'CwwG_G
Cuts at: 0 906 1319 1442 2289 3939 4716
Size: 906 413 123 847 1650 777
Fragments arranged by size:
1650 906 847 777 413 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1312 1807 2526 2896 4716
Size: 1312 495 719 370 1820
Fragments arranged by size:
1820 1312 719 495 370
TatI w'GTAC_w
Cuts at: 0 200 3187 3870 4211 4254 4699 4716
Size: 200 2987 683 341 43 445 17
Fragments arranged by size:
2987 683 445 341 200 43 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 705 737 1854 1886 1960 1992 2086 2118
Size: 705 32 1117 32 74 32 94 32
Cuts at: 2118 2707 2739 4584 4616 4716
Size: 589 32 1845 32 100
Fragments arranged by size:
1845 1117 705 589 100 94 74 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1794 4716
Size: 1794 2922
XbaI T'CTAG_A
Cuts at: 0 3784 4716
Size: 3784 932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 311 436 2562 3081 4716
Size: 311 125 2126 519 1635
Fragments arranged by size:
2126 1635 519 311 125
XmaI C'CCGG_G
Cuts at: 0 14 3548 4716
Size: 14 3534 1168
Fragments arranged by size:
3534 1168 14
XmnI GAAnn'nnTTC
Cuts at: 0 1595 4716
Size: 1595 3121
ZraI GAC'GTC
Cuts at: 0 1234 4716
Size: 1234 3482
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AleI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I
NcoI NdeI NruI NsiI NspI PasI PciI PflMI
PfoI PmlI PpuMI PsiI PspOMI PstI PvuII SacI
SacII SapI SbfI ScaI SexAI SfcI SmaI SmlI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AgeI AhdI AlfI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BglI BmtI Bpu10I
BsiWI BssHII BstZ17I ClaI DrdI EagI EciI FseI
FspI FspAI HindIII HpaI KasI MfeI MluI NaeI
NarI NgoMIV NheI NotI PacI PmeI PpiI PshAI
PspXI PsrI PvuI RsrII SalI SanDI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SwaI XhoI