Restriction map for
pF1KA1310
(Linear) (Six-base) MAPSORT of: KIAA1310.seq Check: 6606 from: 1 to: 2340
KIAA1310.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2005 2340
Size: 2005 335
AatII G_ACGT'C
Cuts at: 0 227 2340
Size: 227 2113
AccI GT'mk_AC
Cuts at: 0 1533 2340
Size: 1533 807
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 673 766 1259 1708 1897 2036 2123
Size: 20 653 93 493 449 189 139 87
Cuts at: 2123 2129 2250 2340
Size: 6 121 90
Fragments arranged by size:
653 493 449 189 139 121 93 90
87 20 6
AflIII A'CryG_T
Cuts at: 0 1695 2340
Size: 1695 645
AhdI GACnn_n'nnGTC
Cuts at: 0 608 2340
Size: 608 1732
AleI CACnn'nnGTG
Cuts at: 0 11 595 2340
Size: 11 584 1745
Fragments arranged by size:
1745 584 11
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 76 1134 2340
Size: 76 1058 1206
Fragments arranged by size:
1206 1058 76
AlwNI CAG_nnn'CTG
Cuts at: 0 543 582 651 724 1686 1714 2011 2340
Size: 543 39 69 73 962 28 297 329
Fragments arranged by size:
962 543 329 297 73 69 39 28
ApoI r'AATT_y
Cuts at: 0 1218 2340
Size: 1218 1122
AvaI C'yCGr_G
Cuts at: 0 973 1448 1893 2340
Size: 973 475 445 447
Fragments arranged by size:
973 475 447 445
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 192 225 2340
Size: 192 33 2115
Fragments arranged by size:
2115 192 33
BanI G'GyrC_C
Cuts at: 0 52 1798 2340
Size: 52 1746 542
Fragments arranged by size:
1746 542 52
BanII G_rGCy'C
Cuts at: 0 1441 2072 2340
Size: 1441 631 268
Fragments arranged by size:
1441 631 268
BbsI GAAGACnn'nnnn_
Cuts at: 0 1195 2340
Size: 1195 1145
BbvCI CC'TCA_GC
Cuts at: 0 725 1424 2260 2340
Size: 725 699 836 80
Fragments arranged by size:
836 725 699 80
BciVI GTATCCnnnnn_n'
Cuts at: 0 555 2340
Size: 555 1785
BglI GCCn_nnn'nGGC
Cuts at: 0 463 2335 2340
Size: 463 1872 5
Fragments arranged by size:
1872 463 5
Bme1580I G_kGCm'C
Cuts at: 0 55 141 162 2340
Size: 55 86 21 2178
Fragments arranged by size:
2178 86 55 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 375 719 2050 2125 2159 2340
Size: 375 344 1331 75 34 181
Fragments arranged by size:
1331 375 344 181 75 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1605 1802 2340
Size: 1605 197 538
Fragments arranged by size:
1605 538 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 828 1030 1178 1427 1575 1599 1988 1991
Size: 828 202 148 249 148 24 389 3
Cuts at: 1991 2133 2340
Size: 142 207
Fragments arranged by size:
828 389 249 207 202 148 148 142
24 3
Bpu10I CC'TnA_GC
Cuts at: 0 725 1424 1520 2260 2340
Size: 725 699 96 740 80
Fragments arranged by size:
740 725 699 96 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1721 2340
Size: 627 1094 619
Fragments arranged by size:
1094 627 619
BsaI GGTCTCn'nnnn_
Cuts at: 0 595 2151 2340
Size: 595 1556 189
Fragments arranged by size:
1556 595 189
BsaBI GATnn'nnATC
Cuts at: 0 823 1310 2340
Size: 823 487 1030
Fragments arranged by size:
1030 823 487
BsaHI Gr'CG_yC
Cuts at: 0 224 2340
Size: 224 2116
BsaWI w'CCGG_w
Cuts at: 0 680 2340
Size: 680 1660
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1058 1088 2340
Size: 1058 30 1252
Fragments arranged by size:
1252 1058 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 341 385 822 1794 2239 2340
Size: 341 44 437 972 445 101
Fragments arranged by size:
972 445 437 341 101 44
BseYI C'CCAG_C
Cuts at: 0 557 1545 2340
Size: 557 988 795
Fragments arranged by size:
988 795 557
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1720 2144 2340
Size: 568 1152 424 196
Fragments arranged by size:
1152 568 424 196
BsiEI CG_ry'CG
Cuts at: 0 1930 2340
Size: 1930 410
BsiHKAI G_wGCw'C
Cuts at: 0 1415 1986 2004 2072 2340
Size: 1415 571 18 68 268
Fragments arranged by size:
1415 571 268 68 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 235 2340
Size: 235 2105
Bsp1286I G_dGCh'C
Cuts at: 0 55 141 162 1415 1441 1986 2004 2072
Size: 55 86 21 1253 26 545 18 68
Cuts at: 2072 2340
Size: 268
Fragments arranged by size:
1253 545 268 86 68 55 26 21
18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1545 2005 2340
Size: 1545 460 335
Fragments arranged by size:
1545 460 335
BsrBI CCG'CTC
Cuts at: 0 1524 2340
Size: 1524 816
BsrFI r'CCGG_y
Cuts at: 0 449 2340
Size: 449 1891
BssSI C'ACGA_G
Cuts at: 0 1418 2340
Size: 1418 922
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1747 2167 2181 2340
Size: 1747 420 14 159
Fragments arranged by size:
1747 420 159 14
BstXI CCAn_nnnn'nTGG
Cuts at: 0 13 365 597 2040 2340
Size: 13 352 232 1443 300
Fragments arranged by size:
1443 352 300 232 13
BstYI r'GATC_y
Cuts at: 0 425 662 1584 1860 2340
Size: 425 237 922 276 480
Fragments arranged by size:
922 480 425 276 237
Bsu36I CC'TnA_GG
Cuts at: 0 1572 2340
Size: 1572 768
BtgI C'CryG_G
Cuts at: 0 1244 2340
Size: 1244 1096
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 810 1499 2340
Size: 810 689 841
Fragments arranged by size:
841 810 689
BtsI GCAGTG_nn'
Cuts at: 0 954 998 1106 2156 2179 2340
Size: 954 44 108 1050 23 161
Fragments arranged by size:
1050 954 161 108 44 23
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 218 253 930 965 2340
Size: 218 35 677 35 1375
Fragments arranged by size:
1375 677 218 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2167 2340
Size: 2167 173
EaeI y'GGCC_r
Cuts at: 0 464 2198 2340
Size: 464 1734 142
Fragments arranged by size:
1734 464 142
EarI CTCTTCn'nnn_
Cuts at: 0 70 742 1684 1793 2340
Size: 70 672 942 109 547
Fragments arranged by size:
942 672 547 109 70
EcoICRI GAG'CTC
Cuts at: 0 2070 2340
Size: 2070 270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 673 766 828 1030 1178 1259 1427
Size: 20 653 93 62 202 148 81 168
Cuts at: 1427 1575 1599 1708 1897 1988 1991 2036 2123
Size: 148 24 109 189 91 3 45 87
Cuts at: 2123 2129 2133 2250 2340
Size: 6 4 117 90
Fragments arranged by size:
653 202 189 168 148 148 117 109
93 91 90 87 81 62 45 24
20 6 4 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1991 2340
Size: 1991 349
EcoO109I rG'GnC_Cy
Cuts at: 0 752 759 1975 2340
Size: 752 7 1216 365
Fragments arranged by size:
1216 752 365 7
EcoRI G'AATT_C
Cuts at: 0 1218 2340
Size: 1218 1122
EcoRV GAT'ATC
Cuts at: 0 2280 2340
Size: 2280 60
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1879 2340
Size: 1879 461
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 24 56 594 626 718 750 1919 1951
Size: 24 32 538 32 92 32 1169 32
Cuts at: 1951 2340
Size: 389
Fragments arranged by size:
1169 538 389 92 32 32 32 32
24
HincII GTy'rAC
Cuts at: 0 381 1352 1534 2340
Size: 381 971 182 806
Fragments arranged by size:
971 806 381 182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 2340
Size: 722 1618
MscI TGG'CCA
Cuts at: 0 466 2200 2340
Size: 466 1734 140
Fragments arranged by size:
1734 466 140
MslI CAynn'nnrTG
Cuts at: 0 11 119 146 172 595 2032 2340
Size: 11 108 27 26 423 1437 308
Fragments arranged by size:
1437 423 308 108 27 26 11
MspA1I CmG'CkG
Cuts at: 0 816 894 1549 1683 1704 1875 1969 2340
Size: 816 78 655 134 21 171 94 371
Fragments arranged by size:
816 655 371 171 134 94 78 21
NcoI C'CATG_G
Cuts at: 0 1244 2340
Size: 1244 1096
NdeI CA'TA_TG
Cuts at: 0 980 2340
Size: 980 1360
NspI r_CATG'y
Cuts at: 0 177 319 379 1699 2340
Size: 177 142 60 1320 641
Fragments arranged by size:
1320 641 177 142 60
PasI CC'CwG_GG
Cuts at: 0 755 2224 2340
Size: 755 1469 116
Fragments arranged by size:
1469 755 116
PciI A'CATG_T
Cuts at: 0 1695 2340
Size: 1695 645
PflMI CCAn_nnn'nTGG
Cuts at: 0 131 2340
Size: 131 2209
PfoI T'CCnGG_A
Cuts at: 0 1256 2340
Size: 1256 1084
PpuMI rG'GwC_Cy
Cuts at: 0 759 2340
Size: 759 1581
PshAI GACnn'nnGTC
Cuts at: 0 1195 2340
Size: 1195 1145
PspXI vC'TCGA_Gb
Cuts at: 0 1893 2340
Size: 1893 447
PstI C_TGCA'G
Cuts at: 0 443 720 1113 2016 2186 2340
Size: 443 277 393 903 170 154
Fragments arranged by size:
903 443 393 277 170 154
PvuII CAG'CTG
Cuts at: 0 894 1549 1683 1875 1969 2340
Size: 894 655 134 192 94 371
Fragments arranged by size:
894 655 371 192 134 94
SacI G_AGCT'C
Cuts at: 0 2072 2340
Size: 2072 268
SalI G'TCGA_C
Cuts at: 0 1532 2340
Size: 1532 808
SanDI GG'GwC_CC
Cuts at: 0 759 2340
Size: 759 1581
SapI GCTCTTCn'nnn_
Cuts at: 0 70 1793 2340
Size: 70 1723 547
Fragments arranged by size:
1723 547 70
SexAI A'CCwGG_T
Cuts at: 0 98 2340
Size: 98 2242
SfcI C'TryA_G
Cuts at: 0 439 716 920 964 1109 2012 2182 2340
Size: 439 277 204 44 145 903 170 158
Fragments arranged by size:
903 439 277 204 170 158 145 44
SmlI C'TyrA_G
Cuts at: 0 606 973 1736 1893 2340
Size: 606 367 763 157 447
Fragments arranged by size:
763 606 447 367 157
SphI G_CATG'C
Cuts at: 0 177 2340
Size: 177 2163
StuI AGG'CCT
Cuts at: 0 730 2258 2340
Size: 730 1528 82
Fragments arranged by size:
1528 730 82
StyI C'CwwG_G
Cuts at: 0 130 1122 1244 1956 1992 2063 2253 2340
Size: 130 992 122 712 36 71 190 87
Fragments arranged by size:
992 712 190 130 122 87 71 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 158 760 943 1944 2178 2340
Size: 158 602 183 1001 234 162
Fragments arranged by size:
1001 602 234 183 162 158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1058 1090 1602 1634 2340
Size: 1058 32 512 32 706
Fragments arranged by size:
1058 706 512 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 362 2340
Size: 362 1978
XhoI C'TCGA_G
Cuts at: 0 973 1893 2340
Size: 973 920 447
Fragments arranged by size:
973 920 447
ZraI GAC'GTC
Cuts at: 0 225 2340
Size: 225 2115
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AleI AlfI
AlwNI ApoI AvaI BaeI BanI BanII BbsI BbvCI
BciVI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI Hin4I HincII MmeI MscI MslI MspA1I
NcoI NdeI NspI PasI PciI PflMI PfoI PpuMI
PshAI PspXI PstI PvuII SacI SalI SanDI SapI
SexAI SfcI SmlI SphI StuI StyI TaqII TstI
XcmI XhoI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AloI ApaI ApaLI
AscI AseI AsiSI AvrII BamHI BbeI BcgI BclI
BfrBI BglII BlpI BmgBI BmtI BsaAI BsiWI BsmI
BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I
ClaI DraI DrdI EagI EciI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PsiI PspOMI PsrI PvuI RsrII SacII
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI TatI Tth111I XbaI XmaI
XmnI