Restriction map for
pF1KA1315
(Linear) (Six-base) MAPSORT of: KIAA1315.seq Check: 1044 from: 1 to: 5943
KIAA1315.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AccI GT'mk_AC
Cuts at: 0 1004 4234 5943
Size: 1004 3230 1709
Fragments arranged by size:
3230 1709 1004
AclI AA'CG_TT
Cuts at: 0 1888 5943
Size: 1888 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 1427 1610 2249 2431 4157 4369 4427
Size: 209 1218 183 639 182 1726 212 58
Cuts at: 4427 4883 5633 5943
Size: 456 750 310
Fragments arranged by size:
1726 1218 750 639 456 310 212 209
183 182 58
AfeI AGC'GCT
Cuts at: 0 129 3754 5943
Size: 129 3625 2189
Fragments arranged by size:
3625 2189 129
AflII C'TTAA_G
Cuts at: 0 64 331 795 3319 3436 4045 5943
Size: 64 267 464 2524 117 609 1898
Fragments arranged by size:
2524 1898 609 464 267 117 64
AflIII A'CryG_T
Cuts at: 0 2770 2958 3119 3141 5943
Size: 2770 188 161 22 2802
Fragments arranged by size:
2802 2770 188 161 22
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 598 5943
Size: 598 5345
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3727 3759 5943
Size: 3727 32 2184
Fragments arranged by size:
3727 2184 32
AlwNI CAG_nnn'CTG
Cuts at: 0 931 1924 2131 5074 5943
Size: 931 993 207 2943 869
Fragments arranged by size:
2943 993 931 869 207
ApoI r'AATT_y
Cuts at: 0 300 2353 2676 2778 2859 3163 3175 3705
Size: 300 2053 323 102 81 304 12 530
Cuts at: 3705 4882 5095 5804 5943
Size: 1177 213 709 139
Fragments arranged by size:
2053 1177 709 530 323 304 300 213
139 102 81 12
AseI AT'TA_AT
Cuts at: 0 2108 3184 5943
Size: 2108 1076 2759
Fragments arranged by size:
2759 2108 1076
BanII G_rGCy'C
Cuts at: 0 2018 4849 5943
Size: 2018 2831 1094
Fragments arranged by size:
2831 2018 1094
BbsI GAAGACnn'nnnn_
Cuts at: 0 1501 1778 1967 3605 4163 5282 5943
Size: 1501 277 189 1638 558 1119 661
Fragments arranged by size:
1638 1501 1119 661 558 277 189
BciVI GTATCCnnnnn_n'
Cuts at: 0 2181 5943
Size: 2181 3762
BclI T'GATC_A
Cuts at: 0 531 1104 4614 5124 5943
Size: 531 573 3510 510 819
Fragments arranged by size:
3510 819 573 531 510
BfrBI ATG'CAT
Cuts at: 0 1362 2004 4462 4587 5360 5943
Size: 1362 642 2458 125 773 583
Fragments arranged by size:
2458 1362 773 642 583 125
BglII A'GATC_T
Cuts at: 0 486 499 1150 3016 4314 5052 5943
Size: 486 13 651 1866 1298 738 891
Fragments arranged by size:
1866 1298 891 738 651 486 13
BmgBI CAC'GTC
Cuts at: 0 2914 4639 5943
Size: 2914 1725 1304
Fragments arranged by size:
2914 1725 1304
BmrI ACTGGGnnnn_n'
Cuts at: 0 5396 5943
Size: 5396 547
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 729 5943
Size: 729 5214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1001 1164 2428 3654 4004 4510 4538 5162
Size: 1001 163 1264 1226 350 506 28 624
Cuts at: 5162 5943
Size: 781
Fragments arranged by size:
1264 1226 1001 781 624 506 350 163
28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2615 4468 5943
Size: 2615 1853 1475
Fragments arranged by size:
2615 1853 1475
BsaI GGTCTCn'nnnn_
Cuts at: 0 967 2544 2586 5895 5943
Size: 967 1577 42 3309 48
Fragments arranged by size:
3309 1577 967 48 42
BsaAI yAC'GTr
Cuts at: 0 5049 5943
Size: 5049 894
BsaBI GATnn'nnATC
Cuts at: 0 566 1299 1939 4361 5522 5876 5943
Size: 566 733 640 2422 1161 354 67
Fragments arranged by size:
2422 1161 733 640 566 354 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1683 1713 5382 5412 5943
Size: 1683 30 3669 30 531
Fragments arranged by size:
3669 1683 531 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 50 5943
Size: 50 5893
BseYI C'CCAG_C
Cuts at: 0 100 3833 5943
Size: 100 3733 2110
Fragments arranged by size:
3733 2110 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 5943
Size: 648 5295
BsiHKAI G_wGCw'C
Cuts at: 0 3055 4425 4849 5166 5943
Size: 3055 1370 424 317 777
Fragments arranged by size:
3055 1370 777 424 317
BsmI GAATG_Cn'
Cuts at: 0 3522 5943
Size: 3522 2421
Bsp1286I G_dGCh'C
Cuts at: 0 2018 3055 4425 4849 5166 5943
Size: 2018 1037 1370 424 317 777
Fragments arranged by size:
2018 1370 1037 777 424 317
BspHI T'CATG_A
Cuts at: 0 2505 4833 5943
Size: 2505 2328 1110
Fragments arranged by size:
2505 2328 1110
BsrDI GCAATG_nn'
Cuts at: 0 1622 4183 5943
Size: 1622 2561 1760
Fragments arranged by size:
2561 1760 1622
BsrFI r'CCGG_y
Cuts at: 0 4188 5943
Size: 4188 1755
BsrGI T'GTAC_A
Cuts at: 0 564 2755 4069 5943
Size: 564 2191 1314 1874
Fragments arranged by size:
2191 1874 1314 564
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4289 5716 5943
Size: 4289 1427 227
Fragments arranged by size:
4289 1427 227
BstBI TT'CG_AA
Cuts at: 0 438 907 5943
Size: 438 469 5036
Fragments arranged by size:
5036 469 438
BstXI CCAn_nnnn'nTGG
Cuts at: 0 5088 5943
Size: 5088 855
BstYI r'GATC_y
Cuts at: 0 179 486 499 1150 3016 4314 5052 5064
Size: 179 307 13 651 1866 1298 738 12
Cuts at: 5064 5664 5943
Size: 600 279
Fragments arranged by size:
1866 1298 738 651 600 307 279 179
13 12
BtgI C'CryG_G
Cuts at: 0 1868 5943
Size: 1868 4075
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4325 5943
Size: 4325 1618
BtsI GCAGTG_nn'
Cuts at: 0 580 660 1925 3984 5943
Size: 580 80 1265 2059 1959
Fragments arranged by size:
2059 1959 1265 580 80
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1746 1781 5943
Size: 1746 35 4162
Fragments arranged by size:
4162 1746 35
DraI TTT'AAA
Cuts at: 0 282 915 1385 1532 3942 4376 4776 5153
Size: 282 633 470 147 2410 434 400 377
Cuts at: 5153 5354 5841 5943
Size: 201 487 102
Fragments arranged by size:
2410 633 487 470 434 400 377 282
201 147 102
DraIII CAC_nnn'GTG
Cuts at: 0 2914 5943
Size: 2914 3029
EarI CTCTTCn'nnn_
Cuts at: 0 170 726 963 1220 1227 1916 2006 3102
Size: 170 556 237 257 7 689 90 1096
Cuts at: 3102 3468 3743 4073 4448 4662 5943
Size: 366 275 330 375 214 1281
Fragments arranged by size:
1281 1096 689 556 375 366 330 275
257 237 214 170 90 7
EcoICRI GAG'CTC
Cuts at: 0 4847 5943
Size: 4847 1096
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 1001 1164 1427 1610 2249 2428 2431
Size: 209 792 163 263 183 639 179 3
Cuts at: 2431 3654 4004 4157 4369 4427 4510 4538 4883
Size: 1223 350 153 212 58 83 28 345
Cuts at: 4883 5162 5633 5943
Size: 279 471 310
Fragments arranged by size:
1223 792 639 471 350 345 310 279
263 212 209 183 179 163 153 83
58 28 3
EcoO109I rG'GnC_Cy
Cuts at: 0 1656 5646 5943
Size: 1656 3990 297
Fragments arranged by size:
3990 1656 297
EcoRI G'AATT_C
Cuts at: 0 2778 5095 5943
Size: 2778 2317 848
Fragments arranged by size:
2778 2317 848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 647 3462 3511 4541 5943
Size: 647 2815 49 1030 1402
Fragments arranged by size:
2815 1402 1030 647 49
HaeII r_GCGC'y
Cuts at: 0 131 3756 5943
Size: 131 3625 2187
Fragments arranged by size:
3625 2187 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 478 510 1768 1800 2380 2412
Size: 67 32 379 32 1258 32 580 32
Cuts at: 2412 2503 2535 4120 4152 4375 4407 4447 4479
Size: 91 32 1585 32 223 32 40 32
Cuts at: 4479 5419 5431 5451 5463 5649 5656 5681 5688
Size: 940 12 20 12 186 7 25 7
Cuts at: 5688 5756 5788 5943
Size: 68 32 155
Fragments arranged by size:
1585 1258 940 580 379 223 186 155
91 68 67 40 32 32 32 32
32 32 32 32 32 25 20 12
12 7 7
HincII GTy'rAC
Cuts at: 0 250 1886 1992 3984 4829 5943
Size: 250 1636 106 1992 845 1114
Fragments arranged by size:
1992 1636 1114 845 250 106
HindIII A'AGCT_T
Cuts at: 0 3433 3465 5943
Size: 3433 32 2478
Fragments arranged by size:
3433 2478 32
HpaI GTT'AAC
Cuts at: 0 3984 5943
Size: 3984 1959
MfeI C'AATT_G
Cuts at: 0 2300 5943
Size: 2300 3643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 3584 4187 4826 5849 5943
Size: 86 3498 603 639 1023 94
Fragments arranged by size:
3498 1023 639 603 94 86
MslI CAynn'nnrTG
Cuts at: 0 512 2510 2660 3118 4199 4430 4919 5943
Size: 512 1998 150 458 1081 231 489 1024
Fragments arranged by size:
1998 1081 1024 512 489 458 231 150
MspA1I CmG'CkG
Cuts at: 0 1852 2881 4032 5943
Size: 1852 1029 1151 1911
Fragments arranged by size:
1911 1852 1151 1029
NcoI C'CATG_G
Cuts at: 0 1868 5943
Size: 1868 4075
NdeI CA'TA_TG
Cuts at: 0 1812 5943
Size: 1812 4131
NsiI A_TGCA'T
Cuts at: 0 1364 2006 4464 4589 5362 5943
Size: 1364 642 2458 125 773 581
Fragments arranged by size:
2458 1364 773 642 581 125
NspI r_CATG'y
Cuts at: 0 2774 2962 3123 3145 3841 4591 5943
Size: 2774 188 161 22 696 750 1352
Fragments arranged by size:
2774 1352 750 696 188 161 22
PacI TTA_AT'TAA
Cuts at: 0 3239 5943
Size: 3239 2704
PasI CC'CwG_GG
Cuts at: 0 5689 5943
Size: 5689 254
PciI A'CATG_T
Cuts at: 0 2770 2958 3119 3141 5943
Size: 2770 188 161 22 2802
Fragments arranged by size:
2802 2770 188 161 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 2475 2914 5943
Size: 2475 439 3029
Fragments arranged by size:
3029 2475 439
PfoI T'CCnGG_A
Cuts at: 0 4531 5943
Size: 4531 1412
PpuMI rG'GwC_Cy
Cuts at: 0 1656 5943
Size: 1656 4287
PsiI TTA'TAA
Cuts at: 0 3614 5943
Size: 3614 2329
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2591 2623 3353 3385 5943
Size: 2591 32 730 32 2558
Fragments arranged by size:
2591 2558 730 32 32
PvuII CAG'CTG
Cuts at: 0 1852 2881 4032 5943
Size: 1852 1029 1151 1911
Fragments arranged by size:
1911 1852 1151 1029
SacI G_AGCT'C
Cuts at: 0 4849 5943
Size: 4849 1094
SapI GCTCTTCn'nnn_
Cuts at: 0 1916 2006 3743 4073 5943
Size: 1916 90 1737 330 1870
Fragments arranged by size:
1916 1870 1737 330 90
ScaI AGT'ACT
Cuts at: 0 630 801 2925 5943
Size: 630 171 2124 3018
Fragments arranged by size:
3018 2124 630 171
SfcI C'TryA_G
Cuts at: 0 1478 2597 5198 5943
Size: 1478 1119 2601 745
Fragments arranged by size:
2601 1478 1119 745
SmlI C'TyrA_G
Cuts at: 0 64 331 795 2630 3319 3436 4045 4447
Size: 64 267 464 1835 689 117 609 402
Cuts at: 4447 5943
Size: 1496
Fragments arranged by size:
1835 1496 689 609 464 402 267 117
64
SnaBI TAC'GTA
Cuts at: 0 5049 5943
Size: 5049 894
SphI G_CATG'C
Cuts at: 0 3841 4591 5943
Size: 3841 750 1352
Fragments arranged by size:
3841 1352 750
SspI AAT'ATT
Cuts at: 0 342 3938 5171 5943
Size: 342 3596 1233 772
Fragments arranged by size:
3596 1233 772 342
StuI AGG'CCT
Cuts at: 0 5349 5943
Size: 5349 594
StyI C'CwwG_G
Cuts at: 0 823 1868 3758 5943
Size: 823 1045 1890 2185
Fragments arranged by size:
2185 1890 1045 823
SwaI ATTT'AAAT
Cuts at: 0 5153 5943
Size: 5153 790
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3919 5943
Size: 3919 2024
TatI w'GTAC_w
Cuts at: 0 564 628 799 2755 2923 3765 3988 4069
Size: 564 64 171 1956 168 842 223 81
Cuts at: 4069 5943
Size: 1874
Fragments arranged by size:
1956 1874 842 564 223 171 168 81
64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5276 5308 5382 5414 5943
Size: 5276 32 74 32 529
Fragments arranged by size:
5276 529 74 32 32
XbaI T'CTAG_A
Cuts at: 0 496 1221 2433 5943
Size: 496 725 1212 3510
Fragments arranged by size:
3510 1212 725 496
XmnI GAAnn'nnTTC
Cuts at: 0 5033 5511 5943
Size: 5033 478 432
Fragments arranged by size:
5033 478 432
Enzymes that do cut:
AccI AclI AcuI AfeI AflII AflIII AlfI AloI
AlwNI ApoI AseI BanII BbsI BciVI BclI BfrBI
BglII BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII EarI EcoICRI
Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
HpaI MfeI MmeI MslI MspA1I NcoI NdeI NsiI
NspI PacI PasI PciI PflMI PfoI PpuMI PsiI
PsrI PvuII SacI SapI ScaI SfcI SmlI SnaBI
SphI SspI StuI StyI SwaI TaqII TatI TstI
XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AgeI AhdI AleI ApaI ApaLI
AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BglI BlpI Bme1580I BmtI Bpu10I BsaHI
BsaWI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BssHII
BssSI BstEII BstZ17I Bsu36I ClaI DrdI EaeI EagI
EciI EcoNI EcoRV FseI FspI FspAI KasI KpnI
MluI MscI NaeI NarI NgoMIV NheI NotI NruI
PmeI PmlI PpiI PshAI PspOMI PspXI PstI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SpeI SrfI Tth111I XcmI XhoI
XmaI ZraI