Restriction map for
pF1KSDA1317
(Linear) (Six-base) MAPSORT of: KIAA1317.seq Check: 9306 from: 1 to: 1284
KIAA1317.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 267 1284
Size: 267 1017
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1094 1284
Size: 1094 190
AhdI GACnn_n'nnGTC
Cuts at: 0 951 1284
Size: 951 333
AlwNI CAG_nnn'CTG
Cuts at: 0 1023 1284
Size: 1023 261
ApaI G_GGCC'C
Cuts at: 0 1048 1284
Size: 1048 236
ApoI r'AATT_y
Cuts at: 0 397 685 1217 1284
Size: 397 288 532 67
Fragments arranged by size:
532 397 288 67
AvaI C'yCGr_G
Cuts at: 0 35 1284
Size: 35 1249
BamHI G'GATC_C
Cuts at: 0 514 1284
Size: 514 770
BanII G_rGCy'C
Cuts at: 0 654 1048 1284
Size: 654 394 236
Fragments arranged by size:
654 394 236
BbsI GAAGACnn'nnnn_
Cuts at: 0 318 816 1284
Size: 318 498 468
Fragments arranged by size:
498 468 318
BciVI GTATCCnnnnn_n'
Cuts at: 0 1204 1284
Size: 1204 80
BclI T'GATC_A
Cuts at: 0 291 1284
Size: 291 993
BglII A'GATC_T
Cuts at: 0 797 1284
Size: 797 487
Bme1580I G_kGCm'C
Cuts at: 0 1048 1284
Size: 1048 236
BmgBI CAC'GTC
Cuts at: 0 914 1284
Size: 914 370
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 981 1284
Size: 981 303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 1101 1204 1284
Size: 654 447 103 80
Fragments arranged by size:
654 447 103 80
BsaI GGTCTCn'nnnn_
Cuts at: 0 632 1284
Size: 632 652
BsaBI GATnn'nnATC
Cuts at: 0 133 1284
Size: 133 1151
BsaWI w'CCGG_w
Cuts at: 0 1097 1284
Size: 1097 187
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1189 1192 1284
Size: 1189 3 92
Fragments arranged by size:
1189 92 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 1284
Size: 504 780
BsiEI CG_ry'CG
Cuts at: 0 480 1284
Size: 480 804
BsmBI CGTCTCn'nnnn_
Cuts at: 0 970 1284
Size: 970 314
Bsp1286I G_dGCh'C
Cuts at: 0 654 1048 1284
Size: 654 394 236
Fragments arranged by size:
654 394 236
BspEI T'CCGG_A
Cuts at: 0 1097 1284
Size: 1097 187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 267 1284
Size: 267 1017
BsrBI CCG'CTC
Cuts at: 0 908 1284
Size: 908 376
BsrDI GCAATG_nn'
Cuts at: 0 927 1284
Size: 927 357
BstAPI GCAn_nnn'nTGC
Cuts at: 0 917 1284
Size: 917 367
BstEII G'GTnAC_C
Cuts at: 0 848 1284
Size: 848 436
BstYI r'GATC_y
Cuts at: 0 514 797 1284
Size: 514 283 487
Fragments arranged by size:
514 487 283
Bsu36I CC'TnA_GG
Cuts at: 0 71 1284
Size: 71 1213
BtsI GCAGTG_nn'
Cuts at: 0 858 1284
Size: 858 426
DraIII CAC_nnn'GTG
Cuts at: 0 741 1284
Size: 741 543
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1095 1284
Size: 1095 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 1094 1101 1204 1284
Size: 654 440 7 103 80
Fragments arranged by size:
654 440 103 80 7
EcoO109I rG'GnC_Cy
Cuts at: 0 1044 1045 1284
Size: 1044 1 239
Fragments arranged by size:
1044 239 1
EcoRI G'AATT_C
Cuts at: 0 397 1284
Size: 397 887
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 665 697 703 735 937 969 1230 1262
Size: 665 32 6 32 202 32 261 32
Cuts at: 1262 1284
Size: 22
Fragments arranged by size:
665 261 202 32 32 32 32 22
6
MfeI C'AATT_G
Cuts at: 0 1169 1284
Size: 1169 115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1086 1284
Size: 1086 198
MslI CAynn'nnrTG
Cuts at: 0 499 1284
Size: 499 785
MspA1I CmG'CkG
Cuts at: 0 1056 1284
Size: 1056 228
PflMI CCAn_nnn'nTGG
Cuts at: 0 741 1023 1284
Size: 741 282 261
Fragments arranged by size:
741 282 261
PfoI T'CCnGG_A
Cuts at: 0 1196 1284
Size: 1196 88
PspOMI G'GGCC_C
Cuts at: 0 1044 1284
Size: 1044 240
PvuII CAG'CTG
Cuts at: 0 1056 1284
Size: 1056 228
SfcI C'TryA_G
Cuts at: 0 18 1284
Size: 18 1266
SmlI C'TyrA_G
Cuts at: 0 35 1284
Size: 35 1249
StyI C'CwwG_G
Cuts at: 0 197 206 429 524 596 1249 1284
Size: 197 9 223 95 72 653 35
Fragments arranged by size:
653 223 197 95 72 35 9
Tth111I GACn'n_nGTC
Cuts at: 0 352 1284
Size: 352 932
XhoI C'TCGA_G
Cuts at: 0 35 1284
Size: 35 1249
XmnI GAAnn'nnTTC
Cuts at: 0 591 1284
Size: 591 693
Enzymes that do cut:
AarI AcuI AhdI AlwNI ApaI ApoI AvaI BamHI
BanII BbsI BciVI BclI BglII Bme1580I BmgBI BplI
BpmI BsaI BsaBI BsaWI BseRI BsgI BsiEI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BstAPI BstEII BstYI
Bsu36I BtsI DraIII EciI Eco57MI EcoO109I EcoRI Hin4I
MfeI MmeI MslI MspA1I PflMI PfoI PspOMI PvuII
SfcI SmlI StyI Tth111I XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BcgI BfrBI BglI BlpI
BmrI BmtI Bpu10I BpuEI BsaAI BsaHI BsaXI BseYI
BsiHKAI BsiWI BsmI BspHI BsrFI BsrGI BssHII BssSI
BstBI BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI
DrdI EaeI EagI EarI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI XbaI XcmI XmaI ZraI