Restriction map for
pF1KSDA1318
(Linear) (Six-base) MAPSORT of: KIAA1318.seq Check: 9527 from: 1 to: 4164
KIAA1318.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AccI GT'mk_AC
Cuts at: 0 87 649 1139 1273 4164
Size: 87 562 490 134 2891
Fragments arranged by size:
2891 562 490 134 87
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 380 815 971 1049 1658 1931 2774
Size: 143 237 435 156 78 609 273 843
Cuts at: 2774 3892 3956 4164
Size: 1118 64 208
Fragments arranged by size:
1118 843 609 435 273 237 208 156
143 78 64
AflIII A'CryG_T
Cuts at: 0 2648 4164
Size: 2648 1516
AhdI GACnn_n'nnGTC
Cuts at: 0 219 4164
Size: 219 3945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3386 4164
Size: 3386 778
AlwNI CAG_nnn'CTG
Cuts at: 0 123 172 289 328 481 598 949 988
Size: 123 49 117 39 153 117 351 39
Cuts at: 988 1027 1105 1183 1261 1300 1480 1558 1597
Size: 39 78 78 78 39 180 78 39
Cuts at: 1597 1909 1948 1987 2026 2065 2329 2407 2482
Size: 312 39 39 39 39 264 78 75
Cuts at: 2482 2563 2641 2680 2719 2797 3109 3148 3235
Size: 81 78 39 39 78 312 39 87
Cuts at: 3235 3304 3343 3841 3972 4164
Size: 69 39 498 131 192
Fragments arranged by size:
498 351 312 312 264 192 180 153
131 123 117 117 87 81 78 78
78 78 78 78 78 75 69 49
39 39 39 39 39 39 39 39
39 39 39 39 39
ApaLI G'TGCA_C
Cuts at: 0 2652 4164
Size: 2652 1512
ApoI r'AATT_y
Cuts at: 0 2238 4164
Size: 2238 1926
AseI AT'TA_AT
Cuts at: 0 580 2857 4164
Size: 580 2277 1307
Fragments arranged by size:
2277 1307 580
AvaI C'yCGr_G
Cuts at: 0 3473 4164
Size: 3473 691
BanII G_rGCy'C
Cuts at: 0 62 1254 1512 1629 1956 2097 2400 2595
Size: 62 1192 258 117 327 141 303 195
Cuts at: 2595 2673 2712 2868 3481 4027 4164
Size: 78 39 156 613 546 137
Fragments arranged by size:
1192 613 546 327 303 258 195 156
141 137 117 78 62 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 716 4099 4164
Size: 716 3383 65
Fragments arranged by size:
3383 716 65
BbvCI CC'TCA_GC
Cuts at: 0 2751 4164
Size: 2751 1413
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3105 3139 4164
Size: 3105 34 1025
Fragments arranged by size:
3105 1025 34
BclI T'GATC_A
Cuts at: 0 4113 4164
Size: 4113 51
BglII A'GATC_T
Cuts at: 0 900 1819 2995 4164
Size: 900 919 1176 1169
Fragments arranged by size:
1176 1169 919 900
Bme1580I G_kGCm'C
Cuts at: 0 297 336 2656 4164
Size: 297 39 2320 1508
Fragments arranged by size:
2320 1508 297 39
BmtI G_CTAG'C
Cuts at: 0 589 4164
Size: 589 3575
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1526 2358 3646 4164
Size: 1526 832 1288 518
Fragments arranged by size:
1526 1288 832 518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 194 272 542 620 659 776 854
Size: 188 6 78 270 78 39 117 78
Cuts at: 854 926 1004 1010 1088 1244 1322 1361 1439
Size: 72 78 6 78 156 78 39 78
Cuts at: 1439 1541 1736 1853 1970 2009 2048 2087 2204
Size: 102 195 117 117 39 39 39 117
Cuts at: 2204 2243 2351 2384 2390 2429 2546 2585 2663
Size: 39 108 33 6 39 117 39 78
Cuts at: 2663 2696 2702 2858 2936 2975 3092 3326 4164
Size: 33 6 156 78 39 117 234 838
Fragments arranged by size:
838 270 234 195 188 156 156 117
117 117 117 117 117 108 102 78
78 78 78 78 78 78 78 78
72 39 39 39 39 39 39 39
39 39 33 33 6 6 6 6
Bpu10I CC'TnA_GC
Cuts at: 0 2751 4164
Size: 2751 1413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2081 3945 4164
Size: 2081 1864 219
Fragments arranged by size:
2081 1864 219
BsaI GGTCTCn'nnnn_
Cuts at: 0 94 2739 2838 3914 4085 4164
Size: 94 2645 99 1076 171 79
Fragments arranged by size:
2645 1076 171 99 94 79
BsaBI GATnn'nnATC
Cuts at: 0 632 1683 1761 3697 4164
Size: 632 1051 78 1936 467
Fragments arranged by size:
1936 1051 632 467 78
BsaWI w'CCGG_w
Cuts at: 0 3282 4164
Size: 3282 882
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1518 3529 4015 4057 4164
Size: 1518 2011 486 42 107
Fragments arranged by size:
2011 1518 486 107 42
BseYI C'CCAG_C
Cuts at: 0 670 848 1041 1254 1590 2436 2673 3435
Size: 670 178 193 213 336 846 237 762
Cuts at: 3435 4164
Size: 729
Fragments arranged by size:
846 762 729 670 336 237 213 193
178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 1181 2798 4164
Size: 209 972 1617 1366
Fragments arranged by size:
1617 1366 972 209
BsiHKAI G_wGCw'C
Cuts at: 0 550 1512 1956 2097 2173 2400 2656 2712
Size: 550 962 444 141 76 227 256 56
Cuts at: 2712 4027 4049 4132 4164
Size: 1315 22 83 32
Fragments arranged by size:
1315 962 550 444 256 227 141 83
76 56 32 22
BsmI GAATG_Cn'
Cuts at: 0 126 4164
Size: 126 4038
Bsp1286I G_dGCh'C
Cuts at: 0 62 297 336 550 1254 1512 1629 1956
Size: 62 235 39 214 704 258 117 327
Cuts at: 1956 2097 2173 2400 2595 2656 2673 2712 2868
Size: 141 76 227 195 61 17 39 156
Cuts at: 2868 3481 4027 4049 4132 4164
Size: 613 546 22 83 32
Fragments arranged by size:
704 613 546 327 258 235 227 214
195 156 141 117 83 76 62 61
39 39 32 22 17
BspEI T'CCGG_A
Cuts at: 0 3282 4164
Size: 3282 882
BspHI T'CATG_A
Cuts at: 0 4110 4164
Size: 4110 54
BsrBI CCG'CTC
Cuts at: 0 891 4164
Size: 891 3273
BsrDI GCAATG_nn'
Cuts at: 0 304 343 382 509 572 689 691 728
Size: 304 39 39 127 63 117 2 37
Cuts at: 728 1040 1235 1276 1430 1532 1649 1727 1922
Size: 312 195 41 154 102 117 78 195
Cuts at: 1922 2039 2537 2968 4164
Size: 117 498 431 1196
Fragments arranged by size:
1196 498 431 312 304 195 195 154
127 117 117 117 102 78 63 41
39 39 37 2
BsrGI T'GTAC_A
Cuts at: 0 135 532 3914 4164
Size: 135 397 3382 250
Fragments arranged by size:
3382 397 250 135
BstXI CCAn_nnnn'nTGG
Cuts at: 0 871 1753 2182 3822 3855 4164
Size: 871 882 429 1640 33 309
Fragments arranged by size:
1640 882 871 429 309 33
BstYI r'GATC_y
Cuts at: 0 900 1819 2178 2431 2551 2995 3661 3863
Size: 900 919 359 253 120 444 666 202
Cuts at: 3863 4164
Size: 301
Fragments arranged by size:
919 900 666 444 359 301 253 202
120
Bsu36I CC'TnA_GG
Cuts at: 0 1674 1791 1869 3228 4164
Size: 1674 117 78 1359 936
Fragments arranged by size:
1674 1359 936 117 78
BtgI C'CryG_G
Cuts at: 0 1120 1448 1667 2828 2906 2945 4007 4164
Size: 1120 328 219 1161 78 39 1062 157
Fragments arranged by size:
1161 1120 1062 328 219 157 78 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 908 1126 1988 3344 4164
Size: 908 218 862 1356 820
Fragments arranged by size:
1356 908 862 820 218
BtsI GCAGTG_nn'
Cuts at: 0 19 1924 2344 2810 4164
Size: 19 1905 420 466 1354
Fragments arranged by size:
1905 1354 466 420 19
ClaI AT'CG_AT
Cuts at: 0 3115 4164
Size: 3115 1049
DraIII CAC_nnn'GTG
Cuts at: 0 3923 4164
Size: 3923 241
EaeI y'GGCC_r
Cuts at: 0 1406 3041 4164
Size: 1406 1635 1123
Fragments arranged by size:
1635 1406 1123
EarI CTCTTCn'nnn_
Cuts at: 0 3452 3491 3578 3932 4164
Size: 3452 39 87 354 232
Fragments arranged by size:
3452 354 232 87 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1151 4164
Size: 1151 3013
EcoICRI GAG'CTC
Cuts at: 0 1510 1954 2095 2398 2710 4025 4164
Size: 1510 444 141 303 312 1315 139
Fragments arranged by size:
1510 1315 444 312 303 141 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 188 194 272 380 542 620 659
Size: 143 45 6 78 108 162 78 39
Cuts at: 659 776 815 854 926 971 1004 1010 1049
Size: 117 39 39 72 45 33 6 39
Cuts at: 1049 1088 1244 1322 1361 1439 1541 1658 1736
Size: 39 156 78 39 78 102 117 78
Cuts at: 1736 1853 1931 1970 2009 2048 2087 2204 2243
Size: 117 78 39 39 39 39 117 39
Cuts at: 2243 2351 2384 2390 2429 2546 2585 2663 2696
Size: 108 33 6 39 117 39 78 33
Cuts at: 2696 2702 2774 2858 2936 2975 3092 3326 3892
Size: 6 72 84 78 39 117 234 566
Cuts at: 3892 3956 4164
Size: 64 208
Fragments arranged by size:
566 234 208 162 156 143 117 117
117 117 117 117 108 108 102 84
78 78 78 78 78 78 78 78
72 72 64 45 45 39 39 39
39 39 39 39 39 39 39 39
39 39 39 33 33 33 6 6
6 6
EcoO109I rG'GnC_Cy
Cuts at: 0 2339 3224 3232 4164
Size: 2339 885 8 932
Fragments arranged by size:
2339 932 885 8
EcoRI G'AATT_C
Cuts at: 0 2238 4164
Size: 2238 1926
EcoRV GAT'ATC
Cuts at: 0 491 764 4164
Size: 491 273 3400
Fragments arranged by size:
3400 491 273
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4123 4164
Size: 4123 41
HaeII r_GCGC'y
Cuts at: 0 3052 4164
Size: 3052 1112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 186 205 218 237 553 585 670 702
Size: 186 19 13 19 316 32 85 32
Cuts at: 702 892 924 1143 1175 1348 1380 1616 1648
Size: 190 32 219 32 173 32 236 32
Cuts at: 1648 1774 1791 1806 1823 1969 2001 2142 2174
Size: 126 17 15 17 146 32 141 32
Cuts at: 2174 2245 2277 2752 2784 3264 3296 3670 3702
Size: 71 32 475 32 480 32 374 32
Cuts at: 3702 4164
Size: 462
Fragments arranged by size:
480 475 462 374 316 236 219 190
186 173 146 141 126 85 71 32
32 32 32 32 32 32 32 32
32 32 32 19 19 17 17 15
13
HincII GTy'rAC
Cuts at: 0 1127 1400 3431 4164
Size: 1127 273 2031 733
Fragments arranged by size:
2031 1127 733 273
HpaI GTT'AAC
Cuts at: 0 1400 4164
Size: 1400 2764
MfeI C'AATT_G
Cuts at: 0 1579 4164
Size: 1579 2585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1537 3841 4164
Size: 1537 2304 323
Fragments arranged by size:
2304 1537 323
MscI TGG'CCA
Cuts at: 0 1408 3043 4164
Size: 1408 1635 1121
Fragments arranged by size:
1635 1408 1121
MslI CAynn'nnrTG
Cuts at: 0 131 3163 3853 4052 4164
Size: 131 3032 690 199 112
Fragments arranged by size:
3032 690 199 131 112
MspA1I CmG'CkG
Cuts at: 0 823 2221 3930 4164
Size: 823 1398 1709 234
Fragments arranged by size:
1709 1398 823 234
NcoI C'CATG_G
Cuts at: 0 1120 1667 2828 2906 2945 4007 4164
Size: 1120 547 1161 78 39 1062 157
Fragments arranged by size:
1161 1120 1062 547 157 78 39
NdeI CA'TA_TG
Cuts at: 0 2804 4164
Size: 2804 1360
NheI G'CTAG_C
Cuts at: 0 585 4164
Size: 585 3579
NspI r_CATG'y
Cuts at: 0 2652 4057 4164
Size: 2652 1405 107
Fragments arranged by size:
2652 1405 107
PasI CC'CwG_GG
Cuts at: 0 418 3666 4164
Size: 418 3248 498
Fragments arranged by size:
3248 498 418
PciI A'CATG_T
Cuts at: 0 2648 4164
Size: 2648 1516
PflMI CCAn_nnn'nTGG
Cuts at: 0 250 520 598 988 1105 1261 1300 1339
Size: 250 270 78 390 117 156 39 39
Cuts at: 1339 1519 1597 2407 2443 2680 2719 4164
Size: 180 78 810 36 237 39 1445
Fragments arranged by size:
1445 810 390 270 250 237 180 156
117 78 78 39 39 39 36
PfoI T'CCnGG_A
Cuts at: 0 636 2181 4164
Size: 636 1545 1983
Fragments arranged by size:
1983 1545 636
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3583 3615 4164
Size: 3583 32 549
Fragments arranged by size:
3583 549 32
PshAI GACnn'nnGTC
Cuts at: 0 2805 3786 4164
Size: 2805 981 378
Fragments arranged by size:
2805 981 378
PstI C_TGCA'G
Cuts at: 0 486 1692 4164
Size: 486 1206 2472
Fragments arranged by size:
2472 1206 486
PvuII CAG'CTG
Cuts at: 0 823 3930 4164
Size: 823 3107 234
Fragments arranged by size:
3107 823 234
SacI G_AGCT'C
Cuts at: 0 1512 1956 2097 2400 2712 4027 4164
Size: 1512 444 141 303 312 1315 137
Fragments arranged by size:
1512 1315 444 312 303 141 137
SapI GCTCTTCn'nnn_
Cuts at: 0 3452 4164
Size: 3452 712
SexAI A'CCwGG_T
Cuts at: 0 3814 4164
Size: 3814 350
SfcI C'TryA_G
Cuts at: 0 84 146 482 824 1688 4164
Size: 84 62 336 342 864 2476
Fragments arranged by size:
2476 864 342 336 84 62
SmaI CCC'GGG
Cuts at: 0 3475 4164
Size: 3475 689
SmlI C'TyrA_G
Cuts at: 0 2096 3960 4164
Size: 2096 1864 204
Fragments arranged by size:
2096 1864 204
SpeI A'CTAG_T
Cuts at: 0 2700 4164
Size: 2700 1464
StyI C'CwwG_G
Cuts at: 0 1120 1667 2828 2906 2945 4007 4119 4164
Size: 1120 547 1161 78 39 1062 112 45
Fragments arranged by size:
1161 1120 1062 547 112 78 45 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 877 1096 1549 1875 2188 4164
Size: 877 219 453 326 313 1976
Fragments arranged by size:
1976 877 453 326 313 219
TatI w'GTAC_w
Cuts at: 0 135 532 3914 4164
Size: 135 397 3382 250
Fragments arranged by size:
3382 397 250 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2163 2195 4164
Size: 2163 32 1969
Fragments arranged by size:
2163 1969 32
Tth111I GACn'n_nGTC
Cuts at: 0 2766 4164
Size: 2766 1398
XbaI T'CTAG_A
Cuts at: 0 903 1378 2602 2992 3196 4164
Size: 903 475 1224 390 204 968
Fragments arranged by size:
1224 968 903 475 390 204
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1242 4164
Size: 1242 2922
XmaI C'CCGG_G
Cuts at: 0 3473 4164
Size: 3473 691
XmnI GAAnn'nnTTC
Cuts at: 0 4106 4164
Size: 4106 58
Enzymes that do cut:
AccI AcuI AflIII AhdI AlfI AlwNI ApaLI ApoI
AseI AvaI BanII BbsI BbvCI BcgI BclI BglII
Bme1580I BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BsrBI BsrDI BsrGI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HpaI MfeI MmeI MscI MslI MspA1I NcoI NdeI
NheI NspI PasI PciI PflMI PfoI PpiI PshAI
PstI PvuII SacI SapI SexAI SfcI SmaI SmlI
SpeI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AloI ApaI AscI AsiSI AvrII BaeI BamHI BanI
BbeI BciVI BfrBI BglI BlpI BmgBI BmrI BsaAI
BsaHI BsaXI BsiEI BsiWI BsmBI BspMI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstZ17I CspCI DraI DrdI
EagI EcoNI FseI FspI FspAI HindIII KasI KpnI
MluI NaeI NarI NgoMIV NotI NruI NsiI PacI
PmeI PmlI PpuMI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SphI SrfI SspI StuI SwaI
XhoI ZraI