Restriction map for
pF1KA1319
(Linear) (Six-base) MAPSORT of: KIAA1319.seq Check: 6887 from: 1 to: 3609
KIAA1319.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AccI GT'mk_AC
Cuts at: 0 41 3217 3548 3609
Size: 41 3176 331 61
Fragments arranged by size:
3176 331 61 41
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 805 1417 1519 1676 2257 2716 2911
Size: 512 293 612 102 157 581 459 195
Cuts at: 2911 3109 3209 3609
Size: 198 100 400
Fragments arranged by size:
612 581 512 459 400 293 198 195
157 102 100
AflII C'TTAA_G
Cuts at: 0 1921 3609
Size: 1921 1688
AflIII A'CryG_T
Cuts at: 0 1359 1548 3609
Size: 1359 189 2061
Fragments arranged by size:
2061 1359 189
AhdI GACnn_n'nnGTC
Cuts at: 0 2249 3609
Size: 2249 1360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 481 1231 1612 3609
Size: 481 750 381 1997
Fragments arranged by size:
1997 750 481 381
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2033 2065 3242 3274 3609
Size: 2033 32 1177 32 335
Fragments arranged by size:
2033 1177 335 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 430 768 1224 2076 2235 2307 2328 2454
Size: 430 338 456 852 159 72 21 126
Cuts at: 2454 2751 2763 2826 2835 3045 3493 3605 3609
Size: 297 12 63 9 210 448 112 4
Fragments arranged by size:
852 456 448 430 338 297 210 159
126 112 72 63 21 12 9 4
ApaI G_GGCC'C
Cuts at: 0 37 1509 2466 2883 3609
Size: 37 1472 957 417 726
Fragments arranged by size:
1472 957 726 417 37
ApaLI G'TGCA_C
Cuts at: 0 848 3609
Size: 848 2761
ApoI r'AATT_y
Cuts at: 0 2200 3535 3609
Size: 2200 1335 74
Fragments arranged by size:
2200 1335 74
AvaI C'yCGr_G
Cuts at: 0 29 578 1415 1850 2246 2899 3609
Size: 29 549 837 435 396 653 710
Fragments arranged by size:
837 710 653 549 435 396 29
BamHI G'GATC_C
Cuts at: 0 441 3609
Size: 441 3168
BanI G'GyrC_C
Cuts at: 0 9 3609
Size: 9 3600
BanII G_rGCy'C
Cuts at: 0 29 37 279 286 1271 1509 2466 2883
Size: 29 8 242 7 985 238 957 417
Cuts at: 2883 3017 3526 3609
Size: 134 509 83
Fragments arranged by size:
985 957 509 417 242 238 134 83
29 8 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 518 2713 3097 3208 3275 3609
Size: 518 2195 384 111 67 334
Fragments arranged by size:
2195 518 384 334 111 67
BbvCI CC'TCA_GC
Cuts at: 0 1468 3004 3609
Size: 1468 1536 605
Fragments arranged by size:
1536 1468 605
BglI GCCn_nnn'nGGC
Cuts at: 0 421 2386 2698 2767 3609
Size: 421 1965 312 69 842
Fragments arranged by size:
1965 842 421 312 69
BlpI GC'TnA_GC
Cuts at: 0 23 2414 2513 2780 3307 3486 3520 3609
Size: 23 2391 99 267 527 179 34 89
Fragments arranged by size:
2391 527 267 179 99 89 34 23
Bme1580I G_kGCm'C
Cuts at: 0 37 100 739 852 1509 2391 2466 2883
Size: 37 63 639 113 657 882 75 417
Cuts at: 2883 3609
Size: 726
Fragments arranged by size:
882 726 657 639 417 113 75 63
37
BmrI ACTGGGnnnn_n'
Cuts at: 0 430 822 3609
Size: 430 392 2787
Fragments arranged by size:
2787 430 392
BmtI G_CTAG'C
Cuts at: 0 478 3609
Size: 478 3131
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 993 1542 2727 3480 3609
Size: 993 549 1185 753 129
Fragments arranged by size:
1185 993 753 549 129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 869 1042 1195 1207 1249 1255 1342
Size: 490 379 173 153 12 42 6 87
Cuts at: 1342 1648 1702 1883 2035 2053 2329 2350 2413
Size: 306 54 181 152 18 276 21 63
Cuts at: 2413 2488 2539 2599 2647 2735 2857 3067 3253
Size: 75 51 60 48 88 122 210 186
Cuts at: 3253 3418 3475 3609
Size: 165 57 134
Fragments arranged by size:
490 379 306 276 210 186 181 173
165 153 152 134 122 88 87 75
63 60 57 54 51 48 42 21
18 12 6
Bpu10I CC'TnA_GC
Cuts at: 0 128 1373 1382 1468 3004 3609
Size: 128 1245 9 86 1536 605
Fragments arranged by size:
1536 1245 605 128 86 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1873 3169 3609
Size: 1873 1296 440
Fragments arranged by size:
1873 1296 440
BsaI GGTCTCn'nnnn_
Cuts at: 0 893 1318 1405 2173 2902 3609
Size: 893 425 87 768 729 707
Fragments arranged by size:
893 768 729 707 425 87
BsaHI Gr'CG_yC
Cuts at: 0 120 3609
Size: 120 3489
BsaWI w'CCGG_w
Cuts at: 0 1904 3609
Size: 1904 1705
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 673 703 1471 1501 2033 2063 3046 3076
Size: 673 30 768 30 532 30 983 30
Cuts at: 3076 3609
Size: 533
Fragments arranged by size:
983 768 673 533 532 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 379 847 858 1014 1120 1204 1248 1474
Size: 379 468 11 156 106 84 44 226
Cuts at: 1474 1531 1699 1711 1804 1816 1930 2347 2374
Size: 57 168 12 93 12 114 417 27
Cuts at: 2374 2422 2485 2536 2953 3421 3609
Size: 48 63 51 417 468 188
Fragments arranged by size:
468 468 417 417 379 226 188 168
156 114 106 93 84 63 57 51
48 44 27 12 12 11
BseYI C'CCAG_C
Cuts at: 0 123 181 1250 1960 2139 2621 2669 2819
Size: 123 58 1069 710 179 482 48 150
Cuts at: 2819 3392 3609
Size: 573 217
Fragments arranged by size:
1069 710 573 482 217 179 150 123
58 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 190 1227 3609
Size: 107 83 1037 2382
Fragments arranged by size:
2382 1037 107 83
BsiHKAI G_wGCw'C
Cuts at: 0 203 279 286 852 1271 1889 2498 3017
Size: 203 76 7 566 419 618 609 519
Cuts at: 3017 3526 3609
Size: 509 83
Fragments arranged by size:
618 609 566 519 509 419 203 83
76 7
BsmI GAATG_Cn'
Cuts at: 0 2349 3609
Size: 2349 1260
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2158 3609
Size: 2158 1451
Bsp1286I G_dGCh'C
Cuts at: 0 29 37 100 203 279 286 739 852
Size: 29 8 63 103 76 7 453 113
Cuts at: 852 1271 1509 1889 2391 2466 2498 2883 3017
Size: 419 238 380 502 75 32 385 134
Cuts at: 3017 3526 3609
Size: 509 83
Fragments arranged by size:
509 502 453 419 385 380 238 134
113 103 83 76 75 63 32 29
8 7
BspEI T'CCGG_A
Cuts at: 0 1904 3609
Size: 1904 1705
BsrBI CCG'CTC
Cuts at: 0 1179 1482 2784 3177 3237 3276 3485 3609
Size: 1179 303 1302 393 60 39 209 124
Fragments arranged by size:
1302 1179 393 303 209 124 60 39
BsrDI GCAATG_nn'
Cuts at: 0 3563 3609
Size: 3563 46
BsrFI r'CCGG_y
Cuts at: 0 2207 3609
Size: 2207 1402
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1224 2604 2763 3609
Size: 1224 1380 159 846
Fragments arranged by size:
1380 1224 846 159
BstBI TT'CG_AA
Cuts at: 0 742 3609
Size: 742 2867
BstYI r'GATC_y
Cuts at: 0 441 1740 2741 3609
Size: 441 1299 1001 868
Fragments arranged by size:
1299 1001 868 441
Bsu36I CC'TnA_GG
Cuts at: 0 270 964 2005 3313 3379 3609
Size: 270 694 1041 1308 66 230
Fragments arranged by size:
1308 1041 694 270 230 66
BtgI C'CryG_G
Cuts at: 0 45 658 838 1070 2951 3609
Size: 45 613 180 232 1881 658
Fragments arranged by size:
1881 658 613 232 180 45
BtsI GCAGTG_nn'
Cuts at: 0 512 933 1628 2165 3571 3609
Size: 512 421 695 537 1406 38
Fragments arranged by size:
1406 695 537 512 421 38
DrdI GACnn_nn'nnGTC
Cuts at: 0 49 3609
Size: 49 3560
EaeI y'GGCC_r
Cuts at: 0 591 2117 2982 3101 3609
Size: 591 1526 865 119 508
Fragments arranged by size:
1526 865 591 508 119
EarI CTCTTCn'nnn_
Cuts at: 0 1124 1196 1424 1793 2087 2351 3185 3347
Size: 1124 72 228 369 294 264 834 162
Cuts at: 3347 3609
Size: 262
Fragments arranged by size:
1124 834 369 294 264 262 228 162
72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2194 3609
Size: 2194 1415
EcoICRI GAG'CTC
Cuts at: 0 277 284 1269 3015 3524 3609
Size: 277 7 985 1746 509 85
Fragments arranged by size:
1746 985 509 277 85 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 512 805 869 1042 1195 1207 1249
Size: 490 22 293 64 173 153 12 42
Cuts at: 1249 1255 1342 1417 1519 1648 1676 1702 1883
Size: 6 87 75 102 129 28 26 181
Cuts at: 1883 2035 2053 2257 2329 2350 2413 2488 2539
Size: 152 18 204 72 21 63 75 51
Cuts at: 2539 2599 2647 2716 2735 2857 2911 3067 3109
Size: 60 48 69 19 122 54 156 42
Cuts at: 3109 3209 3253 3418 3475 3609
Size: 100 44 165 57 134
Fragments arranged by size:
490 293 204 181 173 165 156 153
152 134 129 122 102 100 87 75
75 72 69 64 63 60 57 54
51 48 44 42 42 28 26 22
21 19 18 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 967 996 1513 2407 2437 2470 3609
Size: 967 29 517 894 30 33 1139
Fragments arranged by size:
1139 967 894 517 33 30 29
EcoO109I rG'GnC_Cy
Cuts at: 0 33 34 266 374 427 816 855 1369
Size: 33 1 232 108 53 389 39 514
Cuts at: 1369 1401 1505 1506 2337 2463 2760 2879 3042
Size: 32 104 1 831 126 297 119 163
Cuts at: 3042 3084 3609
Size: 42 525
Fragments arranged by size:
831 525 514 389 297 232 163 126
119 108 104 53 42 39 33 32
1 1
EcoRI G'AATT_C
Cuts at: 0 2200 3535 3609
Size: 2200 1335 74
Fragments arranged by size:
2200 1335 74
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1305 1822 3609
Size: 1305 517 1787
Fragments arranged by size:
1787 1305 517
HaeII r_GCGC'y
Cuts at: 0 687 1287 2676 3609
Size: 687 600 1389 933
Fragments arranged by size:
1389 933 687 600
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 84 116 673 705 1020 1052 2235 2267
Size: 84 32 557 32 315 32 1183 32
Cuts at: 2267 3046 3078 3435 3467 3544 3576 3609
Size: 779 32 357 32 77 32 33
Fragments arranged by size:
1183 779 557 357 315 84 77 33
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 562 1490 3609
Size: 562 928 2119
Fragments arranged by size:
2119 928 562
HindIII A'AGCT_T
Cuts at: 0 3476 3609
Size: 3476 133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1964 2552 3609
Size: 565 1399 588 1057
Fragments arranged by size:
1399 1057 588 565
MscI TGG'CCA
Cuts at: 0 3103 3609
Size: 3103 506
MslI CAynn'nnrTG
Cuts at: 0 3287 3609
Size: 3287 322
MspA1I CmG'CkG
Cuts at: 0 840 1185 1467 1773 1995 2148 2235 2304
Size: 840 345 282 306 222 153 87 69
Cuts at: 2304 2328 2418 2487 2625 2889 3003 3490 3609
Size: 24 90 69 138 264 114 487 119
Fragments arranged by size:
840 487 345 306 282 264 222 153
138 119 114 90 87 69 69 24
NaeI GCC'GGC
Cuts at: 0 2209 3609
Size: 2209 1400
NcoI C'CATG_G
Cuts at: 0 45 3609
Size: 45 3564
NdeI CA'TA_TG
Cuts at: 0 938 3609
Size: 938 2671
NgoMIV G'CCGG_C
Cuts at: 0 2207 3609
Size: 2207 1402
NheI G'CTAG_C
Cuts at: 0 474 3609
Size: 474 3135
NspI r_CATG'y
Cuts at: 0 468 1363 1552 1599 3286 3609
Size: 468 895 189 47 1687 323
Fragments arranged by size:
1687 895 468 323 189 47
PciI A'CATG_T
Cuts at: 0 1359 1548 3609
Size: 1359 189 2061
Fragments arranged by size:
2061 1359 189
PflMI CCAn_nnn'nTGG
Cuts at: 0 82 701 1335 1542 1956 2687 3609
Size: 82 619 634 207 414 731 922
Fragments arranged by size:
922 731 634 619 414 207 82
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 2033 2065 3609
Size: 298 32 1703 32 1544
Fragments arranged by size:
1703 1544 298 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 816 855 1369 1401 3042 3084 3609
Size: 816 39 514 32 1641 42 525
Fragments arranged by size:
1641 816 525 514 42 39 32
PsiI TTA'TAA
Cuts at: 0 2636 3609
Size: 2636 973
PspOMI G'GGCC_C
Cuts at: 0 33 1505 2462 2879 3609
Size: 33 1472 957 417 730
Fragments arranged by size:
1472 957 730 417 33
PspXI vC'TCGA_Gb
Cuts at: 0 2246 3609
Size: 2246 1363
PstI C_TGCA'G
Cuts at: 0 872 1220 1346 1673 1772 1934 2303 2609
Size: 872 348 126 327 99 162 369 306
Cuts at: 2609 2768 2831 3215 3609
Size: 159 63 384 394
Fragments arranged by size:
872 394 384 369 348 327 306 162
159 126 99 63
PvuII CAG'CTG
Cuts at: 0 1185 1467 1773 1995 2235 2328 2625 2889
Size: 1185 282 306 222 240 93 297 264
Cuts at: 2889 3003 3490 3609
Size: 114 487 119
Fragments arranged by size:
1185 487 306 297 282 264 240 222
119 114 93
SacI G_AGCT'C
Cuts at: 0 279 286 1271 3017 3526 3609
Size: 279 7 985 1746 509 83
Fragments arranged by size:
1746 985 509 279 83 7
SacII CC_GC'GG
Cuts at: 0 841 3609
Size: 841 2768
SbfI CC_TGCA'GG
Cuts at: 0 2768 3609
Size: 2768 841
SexAI A'CCwGG_T
Cuts at: 0 2990 3609
Size: 2990 619
SfcI C'TryA_G
Cuts at: 0 868 1111 1216 1342 1669 1768 1930 2299
Size: 868 243 105 126 327 99 162 369
Cuts at: 2299 2605 2764 2827 3181 3211 3592 3609
Size: 306 159 63 354 30 381 17
Fragments arranged by size:
868 381 369 354 327 306 243 162
159 126 105 99 63 30 17
SgrAI Cr'CCGG_yG
Cuts at: 0 2207 3609
Size: 2207 1402
SmaI CCC'GGG
Cuts at: 0 31 580 1417 3609
Size: 31 549 837 2192
Fragments arranged by size:
2192 837 549 31
SmlI C'TyrA_G
Cuts at: 0 1888 1921 2246 2899 3148 3609
Size: 1888 33 325 653 249 461
Fragments arranged by size:
1888 653 461 325 249 33
SphI G_CATG'C
Cuts at: 0 468 1599 3609
Size: 468 1131 2010
Fragments arranged by size:
2010 1131 468
StuI AGG'CCT
Cuts at: 0 415 3609
Size: 415 3194
StyI C'CwwG_G
Cuts at: 0 45 261 2102 2681 3065 3609
Size: 45 216 1841 579 384 544
Fragments arranged by size:
1841 579 544 384 216 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 27 2570 3609
Size: 27 2543 1039
Fragments arranged by size:
2543 1039 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3034 3066 3609
Size: 3034 32 543
Fragments arranged by size:
3034 543 32
Tth111I GACn'n_nGTC
Cuts at: 0 814 3544 3609
Size: 814 2730 65
Fragments arranged by size:
2730 814 65
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1957 2109 2388 3228 3609
Size: 1957 152 279 840 381
Fragments arranged by size:
1957 840 381 279 152
XhoI C'TCGA_G
Cuts at: 0 2246 2899 3609
Size: 2246 653 710
Fragments arranged by size:
2246 710 653
XmaI C'CCGG_G
Cuts at: 0 29 578 1415 3609
Size: 29 549 837 2194
Fragments arranged by size:
2194 837 549 29
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BglI BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrBI BsrDI
BsrFI BstAPI BstBI BstYI Bsu36I BtgI BtsI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NspI PciI
PflMI PpiI PpuMI PsiI PspOMI PspXI PstI PvuII
SacI SacII SbfI SexAI SfcI SgrAI SmaI SmlI
SphI StuI StyI TaqII TstI Tth111I XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AleI AscI
AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI
BfrBI BglII BmgBI BsaAI BsaBI BsiEI BsiWI BspHI
BspMI BsrGI BssHII BssSI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DraIII EagI EcoRV FseI FspI
FspAI HpaI KasI KpnI MfeI MluI NarI NotI
NruI NsiI PacI PasI PfoI PmeI PmlI PshAI
PsrI PvuI RsrII SalI SanDI SapI ScaI SfiI
SfoI SgfI SnaBI SpeI SrfI SspI SwaI TatI
XbaI XmnI ZraI