Restriction map for
pF1KSDA1322
(Linear) (Six-base) MAPSORT of: KIAA1322.seq Check: 7158 from: 1 to: 2079
KIAA1322.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AccI GT'mk_AC
Cuts at: 0 63 2079
Size: 63 2016
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1004 1355 1888 2079
Size: 64 940 351 533 191
Fragments arranged by size:
940 533 351 191 64
AflIII A'CryG_T
Cuts at: 0 93 1463 1814 2079
Size: 93 1370 351 265
Fragments arranged by size:
1370 351 265 93
AlwNI CAG_nnn'CTG
Cuts at: 0 87 1923 2019 2079
Size: 87 1836 96 60
Fragments arranged by size:
1836 96 87 60
ApaI G_GGCC'C
Cuts at: 0 143 2079
Size: 143 1936
ApoI r'AATT_y
Cuts at: 0 1689 2079
Size: 1689 390
AvaI C'yCGr_G
Cuts at: 0 195 289 946 1292 1936 2079
Size: 195 94 657 346 644 143
Fragments arranged by size:
657 644 346 195 143 94
BanI G'GyrC_C
Cuts at: 0 108 2079
Size: 108 1971
BanII G_rGCy'C
Cuts at: 0 143 619 1274 2013 2079
Size: 143 476 655 739 66
Fragments arranged by size:
739 655 476 143 66
BbeI G_GCGC'C
Cuts at: 0 112 2079
Size: 112 1967
BbsI GAAGACnn'nnnn_
Cuts at: 0 173 857 870 2079
Size: 173 684 13 1209
Fragments arranged by size:
1209 684 173 13
BbvCI CC'TCA_GC
Cuts at: 0 668 2079
Size: 668 1411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1968 2002 2079
Size: 1968 34 77
Fragments arranged by size:
1968 77 34
BglII A'GATC_T
Cuts at: 0 1139 2079
Size: 1139 940
BlpI GC'TnA_GC
Cuts at: 0 1236 2079
Size: 1236 843
Bme1580I G_kGCm'C
Cuts at: 0 143 2079
Size: 143 1936
BmgBI CAC'GTC
Cuts at: 0 96 1815 2079
Size: 96 1719 264
Fragments arranged by size:
1719 264 96
BmrI ACTGGGnnnn_n'
Cuts at: 0 1161 1911 2079
Size: 1161 750 168
Fragments arranged by size:
1161 750 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 232 540 595 1251 1408 1718 2079
Size: 166 66 308 55 656 157 310 361
Fragments arranged by size:
656 361 310 308 166 157 66 55
Bpu10I CC'TnA_GC
Cuts at: 0 668 1318 2012 2079
Size: 668 650 694 67
Fragments arranged by size:
694 668 650 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 633 2079
Size: 88 545 1446
Fragments arranged by size:
1446 545 88
BsaAI yAC'GTr
Cuts at: 0 255 2079
Size: 255 1824
BsaHI Gr'CG_yC
Cuts at: 0 109 2079
Size: 109 1970
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 273 685 1969 2079
Size: 273 412 1284 110
Fragments arranged by size:
1284 412 273 110
BseYI C'CCAG_C
Cuts at: 0 969 1058 2079
Size: 969 89 1021
Fragments arranged by size:
1021 969 89
BsiHKAI G_wGCw'C
Cuts at: 0 619 1124 1171 1274 2079
Size: 619 505 47 103 805
Fragments arranged by size:
805 619 505 103 47
BsmI GAATG_Cn'
Cuts at: 0 816 2079
Size: 816 1263
BsmBI CGTCTCn'nnnn_
Cuts at: 0 126 2079
Size: 126 1953
Bsp1286I G_dGCh'C
Cuts at: 0 143 619 1124 1171 1274 2013 2079
Size: 143 476 505 47 103 739 66
Fragments arranged by size:
739 505 476 143 103 66 47
BspHI T'CATG_A
Cuts at: 0 660 1458 2079
Size: 660 798 621
Fragments arranged by size:
798 660 621
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 95 1952 2079
Size: 95 1857 127
Fragments arranged by size:
1857 127 95
BsrBI CCG'CTC
Cuts at: 0 294 357 2079
Size: 294 63 1722
Fragments arranged by size:
1722 294 63
BsrDI GCAATG_nn'
Cuts at: 0 525 2026 2079
Size: 525 1501 53
Fragments arranged by size:
1501 525 53
BsrFI r'CCGG_y
Cuts at: 0 170 1499 2079
Size: 170 1329 580
Fragments arranged by size:
1329 580 170
BssHII G'CGCG_C
Cuts at: 0 314 2079
Size: 314 1765
BssSI C'ACGA_G
Cuts at: 0 546 1267 2079
Size: 546 721 812
Fragments arranged by size:
812 721 546
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1447 2079
Size: 1447 632
BstYI r'GATC_y
Cuts at: 0 1139 1757 2079
Size: 1139 618 322
Fragments arranged by size:
1139 618 322
BtgI C'CryG_G
Cuts at: 0 188 633 1638 2079
Size: 188 445 1005 441
Fragments arranged by size:
1005 445 441 188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 467 1841 1956 2079
Size: 467 1374 115 123
Fragments arranged by size:
1374 467 123 115
BtsI GCAGTG_nn'
Cuts at: 0 1550 2079
Size: 1550 529
DrdI GACnn_nn'nnGTC
Cuts at: 0 495 541 2079
Size: 495 46 1538
Fragments arranged by size:
1538 495 46
EaeI y'GGCC_r
Cuts at: 0 1501 2079
Size: 1501 578
EarI CTCTTCn'nnn_
Cuts at: 0 1829 2079
Size: 1829 250
EcoICRI GAG'CTC
Cuts at: 0 617 1272 2079
Size: 617 655 807
Fragments arranged by size:
807 655 617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 166 232 540 595 1004 1251 1355
Size: 64 102 66 308 55 409 247 104
Cuts at: 1355 1408 1718 1888 2079
Size: 53 310 170 191
Fragments arranged by size:
409 310 308 247 191 170 104 102
66 64 55 53
EcoO109I rG'GnC_Cy
Cuts at: 0 1941 2079
Size: 1941 138
EcoRV GAT'ATC
Cuts at: 0 1740 2079
Size: 1740 339
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1917 2079
Size: 1917 162
FspI TGC'GCA
Cuts at: 0 373 1709 2079
Size: 373 1336 370
Fragments arranged by size:
1336 373 370
HaeII r_GCGC'y
Cuts at: 0 112 311 799 1487 2079
Size: 112 199 488 688 592
Fragments arranged by size:
688 592 488 199 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 891 923 1079 1111 1211 1243
Size: 445 32 414 32 156 32 100 32
Cuts at: 1243 2079
Size: 836
Fragments arranged by size:
836 445 414 156 100 32 32 32
32
HincII GTy'rAC
Cuts at: 0 64 1652 2079
Size: 64 1588 427
Fragments arranged by size:
1588 427 64
KasI G'GCGC_C
Cuts at: 0 108 2079
Size: 108 1971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 302 2079
Size: 296 6 1777
Fragments arranged by size:
1777 296 6
MslI CAynn'nnrTG
Cuts at: 0 1909 2079
Size: 1909 170
MspA1I CmG'CkG
Cuts at: 0 982 1077 2079
Size: 982 95 1002
Fragments arranged by size:
1002 982 95
NarI GG'CG_CC
Cuts at: 0 109 2079
Size: 109 1970
NcoI C'CATG_G
Cuts at: 0 1638 2079
Size: 1638 441
NruI TCG'CGA
Cuts at: 0 1826 2079
Size: 1826 253
NspI r_CATG'y
Cuts at: 0 1467 1530 2079
Size: 1467 63 549
Fragments arranged by size:
1467 549 63
PasI CC'CwG_GG
Cuts at: 0 1857 2079
Size: 1857 222
PciI A'CATG_T
Cuts at: 0 1463 2079
Size: 1463 616
PflMI CCAn_nnn'nTGG
Cuts at: 0 189 2079
Size: 189 1890
PmlI CAC'GTG
Cuts at: 0 255 2079
Size: 255 1824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1828 1860 2079
Size: 1828 32 219
Fragments arranged by size:
1828 219 32
PpuMI rG'GwC_Cy
Cuts at: 0 1941 2079
Size: 1941 138
PshAI GACnn'nnGTC
Cuts at: 0 1283 2079
Size: 1283 796
PspOMI G'GGCC_C
Cuts at: 0 139 2079
Size: 139 1940
PspXI vC'TCGA_Gb
Cuts at: 0 946 2079
Size: 946 1133
PstI C_TGCA'G
Cuts at: 0 244 472 1572 2079
Size: 244 228 1100 507
Fragments arranged by size:
1100 507 244 228
PvuII CAG'CTG
Cuts at: 0 982 1077 2079
Size: 982 95 1002
Fragments arranged by size:
1002 982 95
SacI G_AGCT'C
Cuts at: 0 619 1274 2079
Size: 619 655 805
Fragments arranged by size:
805 655 619
SalI G'TCGA_C
Cuts at: 0 62 2079
Size: 62 2017
SfcI C'TryA_G
Cuts at: 0 240 420 468 1568 2079
Size: 240 180 48 1100 511
Fragments arranged by size:
1100 511 240 180 48
SfoI GGC'GCC
Cuts at: 0 110 2079
Size: 110 1969
SmlI C'TyrA_G
Cuts at: 0 103 612 946 2079
Size: 103 509 334 1133
Fragments arranged by size:
1133 509 334 103
SspI AAT'ATT
Cuts at: 0 692 2079
Size: 692 1387
StyI C'CwwG_G
Cuts at: 0 1298 1638 2079
Size: 1298 340 441
Fragments arranged by size:
1298 441 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1279 2079
Size: 1279 800
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1103 1135 1147 1179 2079
Size: 1103 32 12 32 900
Fragments arranged by size:
1103 900 32 32 12
XbaI T'CTAG_A
Cuts at: 0 1171 1411 2079
Size: 1171 240 668
Fragments arranged by size:
1171 668 240
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 570 2079
Size: 570 1509
XhoI C'TCGA_G
Cuts at: 0 946 2079
Size: 946 1133
XmnI GAAnn'nnTTC
Cuts at: 0 1873 2079
Size: 1873 206
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BseRI
BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstXI BstYI BtgI BtgZI
BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV
FalI FspI HaeII Hin4I HincII KasI MmeI MslI
MspA1I NarI NcoI NruI NspI PasI PciI PflMI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII
SacI SalI SfcI SfoI SmlI SspI StyI TaqII
TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BclI BfrBI BglI BmtI BplI
BsaI BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BspEI
BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DraIII EagI EciI EcoNI EcoRI FseI FspAI
HindIII HpaI KpnI MfeI MluI MscI NaeI NdeI
NgoMIV NheI NotI NsiI PacI PfoI PmeI PsiI
PsrI PvuI RsrII SacII SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TatI Tth111I XmaI ZraI