Restriction map for pF1KSDA1325
 (Linear) (Six-base) MAPSORT of: KIAA1325.seq  Check: 5713  from: 1  to: 1041



KIAA1325.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     904    1041 
   Size:        904     137
 
AflII C'TTAA_G

Cuts at:      0     699    1041 
   Size:        699     342
 
ApaLI G'TGCA_C

Cuts at:      0     632    1041 
   Size:        632     409
 
ApoI r'AATT_y

Cuts at:      0      76     356     394     769    1041 
   Size:         76     280      38     375     272

  Fragments arranged by size: 

                 375     280     272      76      38
 
AvaI C'yCGr_G

Cuts at:      0     535    1041 
   Size:        535     506
 
BanII G_rGCy'C

Cuts at:      0     563     644    1041 
   Size:        563      81     397

  Fragments arranged by size: 

                 563     397      81
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     910    1041 
   Size:        910     131
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     669    1041 
   Size:        669     372
 
Bme1580I G_kGCm'C

Cuts at:      0     636    1041 
   Size:        636     405
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640    1041 
   Size:        640     401
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     719    1041 
   Size:        719     322
 
BseYI C'CCAG_C

Cuts at:      0     309    1041 
   Size:        309     732
 
BsiHKAI G_wGCw'C

Cuts at:      0     636    1041 
   Size:        636     405
 
BsmI GAATG_Cn'

Cuts at:      0     591    1041 
   Size:        591     450
 
Bsp1286I G_dGCh'C

Cuts at:      0     563     636     644    1041 
   Size:        563      73       8     397

  Fragments arranged by size: 

                 563     397      73       8
 
BsrDI GCAATG_nn'

Cuts at:      0     136     615     896    1041 
   Size:        136     479     281     145

  Fragments arranged by size: 

                 479     281     145     136
 
BssSI C'ACGA_G

Cuts at:      0     745    1041 
   Size:        745     296
 
BtsI GCAGTG_nn'

Cuts at:      0     700    1041 
   Size:        700     341
 
EarI CTCTTCn'nnn_

Cuts at:      0     206     414    1041 
   Size:        206     208     627

  Fragments arranged by size: 

                 627     208     206
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640     904    1041 
   Size:        640     264     137

  Fragments arranged by size: 

                 640     264     137
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      51    1041 
   Size:         51     990
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     650     682    1041 
   Size:        650      32     359

  Fragments arranged by size: 

                 650     359      32
 
HincII GTy'rAC

Cuts at:      0       8     199    1041 
   Size:          8     191     842

  Fragments arranged by size: 

                 842     191       8
 
HindIII A'AGCT_T

Cuts at:      0     977    1041 
   Size:        977      64
 
MslI CAynn'nnrTG

Cuts at:      0     835     889    1041 
   Size:        835      54     152

  Fragments arranged by size: 

                 835     152      54
 
MspA1I CmG'CkG

Cuts at:      0     403    1041 
   Size:        403     638
 
NspI r_CATG'y

Cuts at:      0     605    1041 
   Size:        605     436
 
PasI CC'CwG_GG

Cuts at:      0     627    1041 
   Size:        627     414
 
PfoI T'CCnGG_A

Cuts at:      0     863    1041 
   Size:        863     178
 
PstI C_TGCA'G

Cuts at:      0     695    1041 
   Size:        695     346
 
PvuII CAG'CTG

Cuts at:      0     403    1041 
   Size:        403     638
 
SapI GCTCTTCn'nnn_

Cuts at:      0     414    1041 
   Size:        414     627
 
SfcI C'TryA_G

Cuts at:      0     691    1041 
   Size:        691     350
 
SmlI C'TyrA_G

Cuts at:      0     699     734    1041 
   Size:        699      35     307

  Fragments arranged by size: 

                 699     307      35
 
StyI C'CwwG_G

Cuts at:      0     554     563    1041 
   Size:        554       9     478

  Fragments arranged by size: 

                 554     478       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     127    1041 
   Size:        127     914
 
TatI w'GTAC_w

Cuts at:      0     296     647    1041 
   Size:        296     351     394

  Fragments arranged by size: 

                 394     351     296
 
XbaI T'CTAG_A

Cuts at:      0     321    1041 
   Size:        321     720
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     634    1041 
   Size:        634     407
 

 Enzymes that do cut:

     AcuI    AflII    ApaLI     ApoI     AvaI    BanII     BbsI    BciVI
 Bme1580I     BpmI    BpuEI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrDI
    BssSI     BtsI     EarI  Eco57MI     FalI    Hin4I   HincII  HindIII
     MslI   MspA1I     NspI     PasI     PfoI     PstI    PvuII     SapI
     SfcI     SmlI     StyI    TaqII     TatI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XhoI     XmaI     XmnI     ZraI
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