Restriction map for
pF1KA1331
(Linear) (Six-base) MAPSORT of: KIAA1331.seq Check: 6078 from: 1 to: 1767
KIAA1331.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 266 590 1510 1767
Size: 100 166 324 920 257
Fragments arranged by size:
920 324 257 166 100
AgeI A'CCGG_T
Cuts at: 0 50 1767
Size: 50 1717
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 451 1767
Size: 451 1316
AlwNI CAG_nnn'CTG
Cuts at: 0 328 1511 1764 1767
Size: 328 1183 253 3
Fragments arranged by size:
1183 328 253 3
ApaI G_GGCC'C
Cuts at: 0 75 1767
Size: 75 1692
AseI AT'TA_AT
Cuts at: 0 1247 1767
Size: 1247 520
AvaI C'yCGr_G
Cuts at: 0 68 413 1193 1767
Size: 68 345 780 574
Fragments arranged by size:
780 574 345 68
AvrII C'CTAG_G
Cuts at: 0 1137 1767
Size: 1137 630
BamHI G'GATC_C
Cuts at: 0 331 1767
Size: 331 1436
BanI G'GyrC_C
Cuts at: 0 28 57 1410 1767
Size: 28 29 1353 357
Fragments arranged by size:
1353 357 29 28
BanII G_rGCy'C
Cuts at: 0 75 1767
Size: 75 1692
BbsI GAAGACnn'nnnn_
Cuts at: 0 486 1767
Size: 486 1281
BciVI GTATCCnnnnn_n'
Cuts at: 0 934 1767
Size: 934 833
Bme1580I G_kGCm'C
Cuts at: 0 62 75 1767
Size: 62 13 1692
Fragments arranged by size:
1692 62 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 364 1767
Size: 364 1403
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 1767
Size: 970 797
Bpu10I CC'TnA_GC
Cuts at: 0 1059 1767
Size: 1059 708
BsaBI GATnn'nnATC
Cuts at: 0 1571 1767
Size: 1571 196
BsaHI Gr'CG_yC
Cuts at: 0 594 1767
Size: 594 1173
BsaWI w'CCGG_w
Cuts at: 0 50 1724 1767
Size: 50 1674 43
Fragments arranged by size:
1674 50 43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1626 1656 1767
Size: 1626 30 111
Fragments arranged by size:
1626 111 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 628 1211 1767
Size: 628 583 556
Fragments arranged by size:
628 583 556
BseYI C'CCAG_C
Cuts at: 0 761 1767
Size: 761 1006
BsiEI CG_ry'CG
Cuts at: 0 51 55 1767
Size: 51 4 1712
Fragments arranged by size:
1712 51 4
Bsp1286I G_dGCh'C
Cuts at: 0 62 75 1767
Size: 62 13 1692
Fragments arranged by size:
1692 62 13
BspEI T'CCGG_A
Cuts at: 0 1724 1767
Size: 1724 43
BsrDI GCAATG_nn'
Cuts at: 0 1011 1767
Size: 1011 756
BsrFI r'CCGG_y
Cuts at: 0 50 1767
Size: 50 1717
BsrGI T'GTAC_A
Cuts at: 0 2 1767
Size: 2 1765
BssSI C'ACGA_G
Cuts at: 0 1609 1767
Size: 1609 158
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1713 1767
Size: 1713 54
BstBI TT'CG_AA
Cuts at: 0 298 1767
Size: 298 1469
BstEII G'GTnAC_C
Cuts at: 0 1348 1767
Size: 1348 419
BstYI r'GATC_y
Cuts at: 0 331 1508 1767
Size: 331 1177 259
Fragments arranged by size:
1177 331 259
Bsu36I CC'TnA_GG
Cuts at: 0 539 1767
Size: 539 1228
BtgI C'CryG_G
Cuts at: 0 239 1143 1767
Size: 239 904 624
Fragments arranged by size:
904 624 239
BtsI GCAGTG_nn'
Cuts at: 0 1002 1364 1767
Size: 1002 362 403
Fragments arranged by size:
1002 403 362
DraIII CAC_nnn'GTG
Cuts at: 0 174 1767
Size: 174 1593
EaeI y'GGCC_r
Cuts at: 0 489 1767
Size: 489 1278
EarI CTCTTCn'nnn_
Cuts at: 0 76 309 566 764 1726 1767
Size: 76 233 257 198 962 41
Fragments arranged by size:
962 257 233 198 76 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 976 1767
Size: 976 791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 266 590 970 1510 1767
Size: 100 166 324 380 540 257
Fragments arranged by size:
540 380 324 257 166 100
EcoO109I rG'GnC_Cy
Cuts at: 0 72 838 1767
Size: 72 766 929
Fragments arranged by size:
929 766 72
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1190 1767
Size: 1190 577
HaeII r_GCGC'y
Cuts at: 0 93 1767
Size: 93 1674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 888 1767
Size: 888 879
MspA1I CmG'CkG
Cuts at: 0 225 1626 1767
Size: 225 1401 141
Fragments arranged by size:
1401 225 141
NcoI C'CATG_G
Cuts at: 0 239 1143 1767
Size: 239 904 624
Fragments arranged by size:
904 624 239
NspI r_CATG'y
Cuts at: 0 348 1767
Size: 348 1419
PflMI CCAn_nnn'nTGG
Cuts at: 0 761 1767
Size: 761 1006
PfoI T'CCnGG_A
Cuts at: 0 334 1767
Size: 334 1433
PpuMI rG'GwC_Cy
Cuts at: 0 838 1767
Size: 838 929
PspOMI G'GGCC_C
Cuts at: 0 71 1767
Size: 71 1696
PspXI vC'TCGA_Gb
Cuts at: 0 1193 1767
Size: 1193 574
PvuII CAG'CTG
Cuts at: 0 225 1626 1767
Size: 225 1401 141
Fragments arranged by size:
1401 225 141
SapI GCTCTTCn'nnn_
Cuts at: 0 764 1767
Size: 764 1003
ScaI AGT'ACT
Cuts at: 0 1317 1767
Size: 1317 450
SexAI A'CCwGG_T
Cuts at: 0 483 1767
Size: 483 1284
SfcI C'TryA_G
Cuts at: 0 113 1767
Size: 113 1654
SmaI CCC'GGG
Cuts at: 0 70 1767
Size: 70 1697
SmlI C'TyrA_G
Cuts at: 0 1193 1767
Size: 1193 574
SrfI GCCC'GGGC
Cuts at: 0 70 1767
Size: 70 1697
StyI C'CwwG_G
Cuts at: 0 239 1137 1143 1767
Size: 239 898 6 624
Fragments arranged by size:
898 624 239 6
TatI w'GTAC_w
Cuts at: 0 2 604 1315 1767
Size: 2 602 711 452
Fragments arranged by size:
711 602 452 2
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 1626 1658 1767
Size: 416 32 1178 32 109
Fragments arranged by size:
1178 416 109 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 237 1767
Size: 237 1530
XhoI C'TCGA_G
Cuts at: 0 1193 1767
Size: 1193 574
XmaI C'CCGG_G
Cuts at: 0 68 1767
Size: 68 1699
XmnI GAAnn'nnTTC
Cuts at: 0 1403 1748 1767
Size: 1403 345 19
Fragments arranged by size:
1403 345 19
Enzymes that do cut:
AcuI AgeI AlfI AlwNI ApaI AseI AvaI AvrII
BamHI BanI BanII BbsI BciVI Bme1580I BmrI BpmI
Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
Bsp1286I BspEI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtsI DraIII EaeI EarI
EciI Eco57MI EcoO109I FalI HaeII MmeI MspA1I NcoI
NspI PflMI PfoI PpuMI PspOMI PspXI PvuII SapI
ScaI SexAI SfcI SmaI SmlI SrfI StyI TatI
TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AloI ApaLI ApoI AscI AsiSI BaeI
BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BpuEI BsaI BsaAI BsgI BsiHKAI
BsiWI BsmI BsmBI BspHI BspMI BsrBI BssHII BstXI
BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SspI StuI SwaI TaqII Tth111I XbaI ZraI