Restriction map for
pF1KA1332
(Linear) (Six-base) MAPSORT of: KIAA1332.seq Check: 1801 from: 1 to: 2151
KIAA1332.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 1044 1244 1433 2151
Size: 482 562 200 189 718
Fragments arranged by size:
718 562 482 200 189
AfeI AGC'GCT
Cuts at: 0 355 2151
Size: 355 1796
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1945 2151
Size: 1945 206
AlwNI CAG_nnn'CTG
Cuts at: 0 1816 2151
Size: 1816 335
ApaI G_GGCC'C
Cuts at: 0 1008 2151
Size: 1008 1143
AvaI C'yCGr_G
Cuts at: 0 845 1157 1268 2151
Size: 845 312 111 883
Fragments arranged by size:
883 845 312 111
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 217 250 2151
Size: 217 33 1901
Fragments arranged by size:
1901 217 33
BamHI G'GATC_C
Cuts at: 0 482 754 1492 2151
Size: 482 272 738 659
Fragments arranged by size:
738 659 482 272
BanI G'GyrC_C
Cuts at: 0 1019 1660 2151
Size: 1019 641 491
Fragments arranged by size:
1019 641 491
BanII G_rGCy'C
Cuts at: 0 95 1008 1086 1245 2151
Size: 95 913 78 159 906
Fragments arranged by size:
913 906 159 95 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 1050 1739 2151
Size: 1050 689 412
Fragments arranged by size:
1050 689 412
BbvCI CC'TCA_GC
Cuts at: 0 113 2151
Size: 113 2038
BciVI GTATCCnnnnn_n'
Cuts at: 0 1411 2151
Size: 1411 740
BfrBI ATG'CAT
Cuts at: 0 951 2151
Size: 951 1200
BglI GCCn_nnn'nGGC
Cuts at: 0 1862 2151
Size: 1862 289
BglII A'GATC_T
Cuts at: 0 1479 1500 2151
Size: 1479 21 651
Fragments arranged by size:
1479 651 21
Bme1580I G_kGCm'C
Cuts at: 0 1008 1638 1665 1799 2151
Size: 1008 630 27 134 352
Fragments arranged by size:
1008 630 352 134 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 94 827 1091 2151
Size: 94 733 264 1060
Fragments arranged by size:
1060 733 264 94
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1105 1185 1349 1604 2151
Size: 1105 80 164 255 547
Fragments arranged by size:
1105 547 255 164 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 545 1121 1163 1319 1736 2151
Size: 474 71 576 42 156 417 415
Fragments arranged by size:
576 474 417 415 156 71 42
Bpu10I CC'TnA_GC
Cuts at: 0 113 2151
Size: 113 2038
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 964 2151
Size: 814 150 1187
Fragments arranged by size:
1187 814 150
BsaI GGTCTCn'nnnn_
Cuts at: 0 1227 2151
Size: 1227 924
BsaAI yAC'GTr
Cuts at: 0 1626 1941 2151
Size: 1626 315 210
Fragments arranged by size:
1626 315 210
BsaBI GATnn'nnATC
Cuts at: 0 386 1491 2151
Size: 386 1105 660
Fragments arranged by size:
1105 660 386
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1758 1778 1788 1808 2151
Size: 1758 20 10 20 343
Fragments arranged by size:
1758 343 20 20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 130 696 1124 1310 1727 2151
Size: 103 27 566 428 186 417 424
Fragments arranged by size:
566 428 424 417 186 103 27
BseYI C'CCAG_C
Cuts at: 0 1220 1287 2151
Size: 1220 67 864
Fragments arranged by size:
1220 864 67
BsiEI CG_ry'CG
Cuts at: 0 1218 2151
Size: 1218 933
BsmI GAATG_Cn'
Cuts at: 0 951 2151
Size: 951 1200
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1292 1656 2151
Size: 1292 364 495
Fragments arranged by size:
1292 495 364
Bsp1286I G_dGCh'C
Cuts at: 0 95 1008 1086 1245 1638 1665 1799 2151
Size: 95 913 78 159 393 27 134 352
Fragments arranged by size:
913 393 352 159 134 95 78 27
BspHI T'CATG_A
Cuts at: 0 278 2151
Size: 278 1873
BsrBI CCG'CTC
Cuts at: 0 1169 2151
Size: 1169 982
BsrDI GCAATG_nn'
Cuts at: 0 438 777 1847 2151
Size: 438 339 1070 304
Fragments arranged by size:
1070 438 339 304
BsrGI T'GTAC_A
Cuts at: 0 542 1377 2151
Size: 542 835 774
Fragments arranged by size:
835 774 542
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1016 1828 2151
Size: 1016 812 323
Fragments arranged by size:
1016 812 323
BstYI r'GATC_y
Cuts at: 0 482 754 1479 1492 1500 1512 2151
Size: 482 272 725 13 8 12 639
Fragments arranged by size:
725 639 482 272 13 12 8
BtgI C'CryG_G
Cuts at: 0 678 692 1541 2039 2151
Size: 678 14 849 498 112
Fragments arranged by size:
849 678 498 112 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1708 2151
Size: 1708 443
BtsI GCAGTG_nn'
Cuts at: 0 805 1788 2151
Size: 805 983 363
Fragments arranged by size:
983 805 363
EaeI y'GGCC_r
Cuts at: 0 2 580 699 852 1215 1628 2151
Size: 2 578 119 153 363 413 523
Fragments arranged by size:
578 523 413 363 153 119 2
EagI C'GGCC_G
Cuts at: 0 1215 2151
Size: 1215 936
EarI CTCTTCn'nnn_
Cuts at: 0 149 989 1365 2151
Size: 149 840 376 786
Fragments arranged by size:
840 786 376 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 482 545 1044 1121 1163 1244 1319
Size: 474 8 63 499 77 42 81 75
Cuts at: 1319 1433 1736 2151
Size: 114 303 415
Fragments arranged by size:
499 474 415 303 114 81 77 75
63 42 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1808 1820 2151
Size: 1808 12 331
Fragments arranged by size:
1808 331 12
EcoO109I rG'GnC_Cy
Cuts at: 0 503 783 1004 1005 1708 1813 2151
Size: 503 280 221 1 703 105 338
Fragments arranged by size:
703 503 338 280 221 105 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1297 2151
Size: 1297 854
HaeII r_GCGC'y
Cuts at: 0 357 2151
Size: 357 1794
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 63 95 2151
Size: 63 32 2056
Fragments arranged by size:
2056 63 32
HincII GTy'rAC
Cuts at: 0 1212 2151
Size: 1212 939
HpaI GTT'AAC
Cuts at: 0 1212 2151
Size: 1212 939
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 510 1651 1992 2151
Size: 122 388 1141 341 159
Fragments arranged by size:
1141 388 341 159 122
MscI TGG'CCA
Cuts at: 0 4 701 854 1630 2151
Size: 4 697 153 776 521
Fragments arranged by size:
776 697 521 153 4
MslI CAynn'nnrTG
Cuts at: 0 40 1933 2151
Size: 40 1893 218
Fragments arranged by size:
1893 218 40
MspA1I CmG'CkG
Cuts at: 0 428 1220 1348 2151
Size: 428 792 128 803
Fragments arranged by size:
803 792 428 128
NcoI C'CATG_G
Cuts at: 0 678 692 1541 2151
Size: 678 14 849 610
Fragments arranged by size:
849 678 610 14
NsiI A_TGCA'T
Cuts at: 0 953 2151
Size: 953 1198
NspI r_CATG'y
Cuts at: 0 374 951 2151
Size: 374 577 1200
Fragments arranged by size:
1200 577 374
PasI CC'CwG_GG
Cuts at: 0 1238 1810 2151
Size: 1238 572 341
Fragments arranged by size:
1238 572 341
PfoI T'CCnGG_A
Cuts at: 0 290 333 1335 2151
Size: 290 43 1002 816
Fragments arranged by size:
1002 816 290 43
PmlI CAC'GTG
Cuts at: 0 1626 2151
Size: 1626 525
PpuMI rG'GwC_Cy
Cuts at: 0 503 783 2151
Size: 503 280 1368
Fragments arranged by size:
1368 503 280
PspOMI G'GGCC_C
Cuts at: 0 1004 2151
Size: 1004 1147
PspXI vC'TCGA_Gb
Cuts at: 0 845 2151
Size: 845 1306
PstI C_TGCA'G
Cuts at: 0 1725 2151
Size: 1725 426
PvuII CAG'CTG
Cuts at: 0 428 1348 2151
Size: 428 920 803
Fragments arranged by size:
920 803 428
SapI GCTCTTCn'nnn_
Cuts at: 0 989 1365 2151
Size: 989 376 786
Fragments arranged by size:
989 786 376
ScaI AGT'ACT
Cuts at: 0 1398 2107 2151
Size: 1398 709 44
Fragments arranged by size:
1398 709 44
SfcI C'TryA_G
Cuts at: 0 1721 2151
Size: 1721 430
SmaI CCC'GGG
Cuts at: 0 1270 2151
Size: 1270 881
SmlI C'TyrA_G
Cuts at: 0 793 845 979 2151
Size: 793 52 134 1172
Fragments arranged by size:
1172 793 134 52
SphI G_CATG'C
Cuts at: 0 374 2151
Size: 374 1777
StyI C'CwwG_G
Cuts at: 0 678 692 961 1541 1821 1961 2151
Size: 678 14 269 580 280 140 190
Fragments arranged by size:
678 580 280 269 190 140 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 113 1342 2151
Size: 113 1229 809
Fragments arranged by size:
1229 809 113
TatI w'GTAC_w
Cuts at: 0 542 1377 1396 2105 2129 2151
Size: 542 835 19 709 24 22
Fragments arranged by size:
835 709 542 24 22 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1034 1066 1778 1810 2151
Size: 1034 32 712 32 341
Fragments arranged by size:
1034 712 341 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1072 1548 2151
Size: 1072 476 603
Fragments arranged by size:
1072 603 476
XhoI C'TCGA_G
Cuts at: 0 845 2151
Size: 845 1306
XmaI C'CCGG_G
Cuts at: 0 1268 2151
Size: 1268 883
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApaI AvaI BaeI BamHI
BanI BanII BbsI BbvCI BciVI BfrBI BglI BglII
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaXI BseRI BseYI BsiEI BsmI BsmBI Bsp1286I
BspHI BsrBI BsrDI BsrGI BstXI BstYI BtgI BtgZI
BtsI EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII HpaI MmeI MscI MslI MspA1I
NcoI NsiI NspI PasI PfoI PmlI PpuMI PspOMI
PspXI PstI PvuII SapI ScaI SfcI SmaI SmlI
SphI StyI TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BbeI BcgI BclI BlpI BmgBI BmtI BsaHI
BsaWI BsgI BsiHKAI BsiWI BspEI BspMI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI HindIII KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PciI
PflMI PmeI PpiI PshAI PsiI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XmnI ZraI