Restriction map for
pF1KSDA1334
(Linear) (Six-base) MAPSORT of: KIAA1334.seq Check: 91 from: 1 to: 2940
KIAA1334.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
Acc65I G'GTAC_C
Cuts at: 0 2349 2940
Size: 2349 591
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 1043 1364 2917 2940
Size: 301 742 321 1553 23
Fragments arranged by size:
1553 742 321 301 23
AflII C'TTAA_G
Cuts at: 0 2260 2940
Size: 2260 680
AflIII A'CryG_T
Cuts at: 0 192 2066 2940
Size: 192 1874 874
Fragments arranged by size:
1874 874 192
AleI CACnn'nnGTG
Cuts at: 0 149 2940
Size: 149 2791
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2686 2718 2940
Size: 2686 32 222
Fragments arranged by size:
2686 222 32
AlwNI CAG_nnn'CTG
Cuts at: 0 997 1299 1614 2751 2940
Size: 997 302 315 1137 189
Fragments arranged by size:
1137 997 315 302 189
ApaI G_GGCC'C
Cuts at: 0 1186 2940
Size: 1186 1754
ApoI r'AATT_y
Cuts at: 0 695 1289 2563 2940
Size: 695 594 1274 377
Fragments arranged by size:
1274 695 594 377
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2341 2374 2940
Size: 2341 33 566
Fragments arranged by size:
2341 566 33
BanI G'GyrC_C
Cuts at: 0 2349 2940
Size: 2349 591
BanII G_rGCy'C
Cuts at: 0 430 1186 1342 2617 2940
Size: 430 756 156 1275 323
Fragments arranged by size:
1275 756 430 323 156
BbsI GAAGACnn'nnnn_
Cuts at: 0 2351 2940
Size: 2351 589
BbvCI CC'TCA_GC
Cuts at: 0 1441 1573 1880 2940
Size: 1441 132 307 1060
Fragments arranged by size:
1441 1060 307 132
BciVI GTATCCnnnnn_n'
Cuts at: 0 646 2940
Size: 646 2294
BfrBI ATG'CAT
Cuts at: 0 302 1624 2844 2940
Size: 302 1322 1220 96
Fragments arranged by size:
1322 1220 302 96
BglII A'GATC_T
Cuts at: 0 497 1038 1317 2940
Size: 497 541 279 1623
Fragments arranged by size:
1623 541 497 279
BlpI GC'TnA_GC
Cuts at: 0 1970 2940
Size: 1970 970
Bme1580I G_kGCm'C
Cuts at: 0 1186 2940
Size: 1186 1754
BmrI ACTGGGnnnn_n'
Cuts at: 0 843 2940
Size: 843 2097
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1449 2015 2940
Size: 1449 566 925
Fragments arranged by size:
1449 925 566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1996 2323 2359 2647 2767 2773 2940
Size: 1996 327 36 288 120 6 167
Fragments arranged by size:
1996 327 288 167 120 36 6
Bpu10I CC'TnA_GC
Cuts at: 0 383 1144 1441 1573 1880 2576 2940
Size: 383 761 297 132 307 696 364
Fragments arranged by size:
761 696 383 364 307 297 132
BsaI GGTCTCn'nnnn_
Cuts at: 0 2487 2940
Size: 2487 453
BsaAI yAC'GTr
Cuts at: 0 195 2940
Size: 195 2745
BsaWI w'CCGG_w
Cuts at: 0 253 2940
Size: 253 2687
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 515 545 873 903 2686 2716 2940
Size: 515 30 328 30 1783 30 224
Fragments arranged by size:
1783 515 328 224 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1492 2940
Size: 1492 1448
BseYI C'CCAG_C
Cuts at: 0 330 2141 2705 2940
Size: 330 1811 564 235
Fragments arranged by size:
1811 564 330 235
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 644 1525 2881 2940
Size: 424 220 881 1356 59
Fragments arranged by size:
1356 881 424 220 59
BsiHKAI G_wGCw'C
Cuts at: 0 1342 2617 2940
Size: 1342 1275 323
Fragments arranged by size:
1342 1275 323
BsmI GAATG_Cn'
Cuts at: 0 205 304 1626 2806 2846 2940
Size: 205 99 1322 1180 40 94
Fragments arranged by size:
1322 1180 205 99 94 40
Bsp1286I G_dGCh'C
Cuts at: 0 430 1186 1342 2617 2940
Size: 430 756 156 1275 323
Fragments arranged by size:
1275 756 430 323 156
BspHI T'CATG_A
Cuts at: 0 212 1823 2940
Size: 212 1611 1117
Fragments arranged by size:
1611 1117 212
BsrDI GCAATG_nn'
Cuts at: 0 2351 2940
Size: 2351 589
BsrFI r'CCGG_y
Cuts at: 0 62 2940
Size: 62 2878
BsrGI T'GTAC_A
Cuts at: 0 477 1952 2940
Size: 477 1475 988
Fragments arranged by size:
1475 988 477
BssSI C'ACGA_G
Cuts at: 0 1567 2940
Size: 1567 1373
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1066 1614 2089 2940
Size: 1066 548 475 851
Fragments arranged by size:
1066 851 548 475
BstYI r'GATC_y
Cuts at: 0 497 1038 1317 1611 2940
Size: 497 541 279 294 1329
Fragments arranged by size:
1329 541 497 294 279
Bsu36I CC'TnA_GG
Cuts at: 0 205 2940
Size: 205 2735
BtgI C'CryG_G
Cuts at: 0 77 2940
Size: 77 2863
BtsI GCAGTG_nn'
Cuts at: 0 109 2940
Size: 109 2831
DraI TTT'AAA
Cuts at: 0 740 2940
Size: 740 2200
DraIII CAC_nnn'GTG
Cuts at: 0 226 2940
Size: 226 2714
DrdI GACnn_nn'nnGTC
Cuts at: 0 1282 2067 2940
Size: 1282 785 873
Fragments arranged by size:
1282 873 785
EarI CTCTTCn'nnn_
Cuts at: 0 22 870 1321 1580 1715 1984 2315 2622
Size: 22 848 451 259 135 269 331 307
Cuts at: 2622 2940
Size: 318
Fragments arranged by size:
848 451 331 318 307 269 259 135
22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1147 2812 2940
Size: 1147 1665 128
Fragments arranged by size:
1665 1147 128
EcoICRI GAG'CTC
Cuts at: 0 1340 2615 2940
Size: 1340 1275 325
Fragments arranged by size:
1340 1275 325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 1043 1364 1996 2323 2359 2647 2767
Size: 301 742 321 632 327 36 288 120
Cuts at: 2767 2773 2917 2940
Size: 6 144 23
Fragments arranged by size:
742 632 327 321 301 288 144 120
36 23 6
EcoRI G'AATT_C
Cuts at: 0 695 1289 2940
Size: 695 594 1651
Fragments arranged by size:
1651 695 594
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1513 2706 2940
Size: 1513 1193 234
Fragments arranged by size:
1513 1193 234
HaeII r_GCGC'y
Cuts at: 0 266 2761 2940
Size: 266 2495 179
Fragments arranged by size:
2495 266 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 515 547 760 792 1030 1062 1213 1245
Size: 515 32 213 32 238 32 151 32
Cuts at: 1245 2080 2112 2812 2844 2940
Size: 835 32 700 32 96
Fragments arranged by size:
835 700 515 238 213 151 96 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 345 2138 2940
Size: 345 1793 802
Fragments arranged by size:
1793 802 345
HindIII A'AGCT_T
Cuts at: 0 1146 2940
Size: 1146 1794
KpnI G_GTAC'C
Cuts at: 0 2353 2940
Size: 2353 587
MfeI C'AATT_G
Cuts at: 0 2056 2940
Size: 2056 884
MslI CAynn'nnrTG
Cuts at: 0 149 2940
Size: 149 2791
MspA1I CmG'CkG
Cuts at: 0 379 465 1950 2017 2751 2793 2940
Size: 379 86 1485 67 734 42 147
Fragments arranged by size:
1485 734 379 147 86 67 42
NsiI A_TGCA'T
Cuts at: 0 304 1626 2846 2940
Size: 304 1322 1220 94
Fragments arranged by size:
1322 1220 304 94
PacI TTA_AT'TAA
Cuts at: 0 614 2940
Size: 614 2326
PasI CC'CwG_GG
Cuts at: 0 1191 2940
Size: 1191 1749
PflMI CCAn_nnn'nTGG
Cuts at: 0 44 2940
Size: 44 2896
PfoI T'CCnGG_A
Cuts at: 0 512 538 2940
Size: 512 26 2402
Fragments arranged by size:
2402 512 26
PmlI CAC'GTG
Cuts at: 0 195 2940
Size: 195 2745
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2167 2199 2686 2718 2940
Size: 2167 32 487 32 222
Fragments arranged by size:
2167 487 222 32 32
PspOMI G'GGCC_C
Cuts at: 0 1182 2940
Size: 1182 1758
PstI C_TGCA'G
Cuts at: 0 74 1619 2229 2765 2940
Size: 74 1545 610 536 175
Fragments arranged by size:
1545 610 536 175 74
PvuII CAG'CTG
Cuts at: 0 1950 2017 2751 2793 2940
Size: 1950 67 734 42 147
Fragments arranged by size:
1950 734 147 67 42
SacI G_AGCT'C
Cuts at: 0 1342 2617 2940
Size: 1342 1275 323
Fragments arranged by size:
1342 1275 323
SapI GCTCTTCn'nnn_
Cuts at: 0 2622 2940
Size: 2622 318
ScaI AGT'ACT
Cuts at: 0 987 2894 2940
Size: 987 1907 46
Fragments arranged by size:
1907 987 46
SexAI A'CCwGG_T
Cuts at: 0 1200 2940
Size: 1200 1740
SfcI C'TryA_G
Cuts at: 0 70 989 1321 1363 1615 2225 2761 2940
Size: 70 919 332 42 252 610 536 179
Fragments arranged by size:
919 610 536 332 252 179 70 42
SmlI C'TyrA_G
Cuts at: 0 2260 2940
Size: 2260 680
SspI AAT'ATT
Cuts at: 0 2511 2940
Size: 2511 429
StuI AGG'CCT
Cuts at: 0 2689 2940
Size: 2689 251
StyI C'CwwG_G
Cuts at: 0 1124 2940
Size: 1124 1816
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1137 1183 1513 2345 2940
Size: 1137 46 330 832 595
Fragments arranged by size:
1137 832 595 330 46
TatI w'GTAC_w
Cuts at: 0 477 985 1952 2892 2940
Size: 477 508 967 940 48
Fragments arranged by size:
967 940 508 477 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 534 566 2940
Size: 534 32 2374
Fragments arranged by size:
2374 534 32
Tth111I GACn'n_nGTC
Cuts at: 0 772 2940
Size: 772 2168
XbaI T'CTAG_A
Cuts at: 0 2001 2940
Size: 2001 939
XmnI GAAnn'nnTTC
Cuts at: 0 2510 2940
Size: 2510 430
Enzymes that do cut:
Acc65I AcuI AflII AflIII AleI AloI AlwNI ApaI
ApoI BaeI BanI BanII BbsI BbvCI BciVI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BsrFI BsrGI BssSI BstAPI BstYI
Bsu36I BtgI BtsI DraI DraIII DrdI EarI EciI
EcoICRI Eco57MI EcoRI FalI HaeII Hin4I HincII HindIII
KpnI MfeI MslI MspA1I NsiI PacI PasI PflMI
PfoI PmlI PpiI PspOMI PstI PvuII SacI SapI
ScaI SexAI SfcI SmlI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AgeI AhdI AlfI
ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbeI
BcgI BclI BglI BmgBI BmtI BpuEI BsaBI BsaHI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BssHII BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI EaeI EagI
EcoNI EcoO109I EcoRV FseI FspI FspAI HpaI KasI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PciI PmeI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI XcmI XhoI XmaI ZraI