Restriction map for
pF1KA1336
(Linear) (Six-base) MAPSORT of: KIAA1336.seq Check: 1823 from: 1 to: 3543
KIAA1336.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AccI GT'mk_AC
Cuts at: 0 468 1590 3040 3543
Size: 468 1122 1450 503
Fragments arranged by size:
1450 1122 503 468
Acc65I G'GTAC_C
Cuts at: 0 635 3543
Size: 635 2908
AclI AA'CG_TT
Cuts at: 0 1225 3543
Size: 1225 2318
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 2104 2129 2255 3166 3543
Size: 481 1623 25 126 911 377
Fragments arranged by size:
1623 911 481 377 126 25
AfeI AGC'GCT
Cuts at: 0 2197 3543
Size: 2197 1346
AflIII A'CryG_T
Cuts at: 0 2895 3543
Size: 2895 648
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 64 96 3543
Size: 64 32 3447
Fragments arranged by size:
3447 64 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2127 2358 2629 3515 3543
Size: 2127 231 271 886 28
Fragments arranged by size:
2127 886 271 231 28
ApoI r'AATT_y
Cuts at: 0 23 561 626 1433 3543
Size: 23 538 65 807 2110
Fragments arranged by size:
2110 807 538 65 23
AseI AT'TA_AT
Cuts at: 0 1394 3543
Size: 1394 2149
AvaI C'yCGr_G
Cuts at: 0 1721 2284 3543
Size: 1721 563 1259
Fragments arranged by size:
1721 1259 563
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2660 2693 3543
Size: 2660 33 850
Fragments arranged by size:
2660 850 33
BamHI G'GATC_C
Cuts at: 0 1266 1512 1869 1959 3543
Size: 1266 246 357 90 1584
Fragments arranged by size:
1584 1266 357 246 90
BanI G'GyrC_C
Cuts at: 0 635 1570 2096 3543
Size: 635 935 526 1447
Fragments arranged by size:
1447 935 635 526
BbeI G_GCGC'C
Cuts at: 0 2100 3543
Size: 2100 1443
BbsI GAAGACnn'nnnn_
Cuts at: 0 1068 1846 3154 3182 3323 3543
Size: 1068 778 1308 28 141 220
Fragments arranged by size:
1308 1068 778 220 141 28
BciVI GTATCCnnnnn_n'
Cuts at: 0 69 1077 3543
Size: 69 1008 2466
Fragments arranged by size:
2466 1008 69
BclI T'GATC_A
Cuts at: 0 326 3543
Size: 326 3217
BfrBI ATG'CAT
Cuts at: 0 1039 2056 2761 3067 3543
Size: 1039 1017 705 306 476
Fragments arranged by size:
1039 1017 705 476 306
BglII A'GATC_T
Cuts at: 0 386 3197 3543
Size: 386 2811 346
Fragments arranged by size:
2811 386 346
BlpI GC'TnA_GC
Cuts at: 0 2493 3543
Size: 2493 1050
BmrI ACTGGGnnnn_n'
Cuts at: 0 167 3543
Size: 167 3376
BmtI G_CTAG'C
Cuts at: 0 3214 3543
Size: 3214 329
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3219 3543
Size: 3219 324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 2335 3543
Size: 626 1709 1208
Fragments arranged by size:
1709 1208 626
Bpu10I CC'TnA_GC
Cuts at: 0 13 3491 3543
Size: 13 3478 52
Fragments arranged by size:
3478 52 13
BsaI GGTCTCn'nnnn_
Cuts at: 0 1129 1415 2012 3277 3543
Size: 1129 286 597 1265 266
Fragments arranged by size:
1265 1129 597 286 266
BsaAI yAC'GTr
Cuts at: 0 657 765 2896 3543
Size: 657 108 2131 647
Fragments arranged by size:
2131 657 647 108
BsaBI GATnn'nnATC
Cuts at: 0 437 1181 3543
Size: 437 744 2362
Fragments arranged by size:
2362 744 437
BsaHI Gr'CG_yC
Cuts at: 0 2097 3543
Size: 2097 1446
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2339 2369 3543
Size: 2339 30 1174
Fragments arranged by size:
2339 1174 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 334 1210 3543
Size: 334 876 2333
Fragments arranged by size:
2333 876 334
BseYI C'CCAG_C
Cuts at: 0 2963 3543
Size: 2963 580
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 905 3125 3483 3543
Size: 877 28 2220 358 60
Fragments arranged by size:
2220 877 358 60 28
BsiHKAI G_wGCw'C
Cuts at: 0 2028 3543
Size: 2028 1515
BsmI GAATG_Cn'
Cuts at: 0 375 2457 2964 3543
Size: 375 2082 507 579
Fragments arranged by size:
2082 579 507 375
BsmBI CGTCTCn'nnnn_
Cuts at: 0 124 3543
Size: 124 3419
Bsp1286I G_dGCh'C
Cuts at: 0 2028 3543
Size: 2028 1515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2246 2680 3543
Size: 2246 434 863
Fragments arranged by size:
2246 863 434
BsrDI GCAATG_nn'
Cuts at: 0 617 1310 1835 3543
Size: 617 693 525 1708
Fragments arranged by size:
1708 693 617 525
BsrGI T'GTAC_A
Cuts at: 0 2457 2469 3543
Size: 2457 12 1074
Fragments arranged by size:
2457 1074 12
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2629 3470 3543
Size: 2629 841 73
Fragments arranged by size:
2629 841 73
BstYI r'GATC_y
Cuts at: 0 386 1266 1512 1869 1959 2130 2301 2368
Size: 386 880 246 357 90 171 171 67
Cuts at: 2368 2740 3197 3543
Size: 372 457 346
Fragments arranged by size:
880 457 386 372 357 346 246 171
171 90 67
BstZ17I GTA'TAC
Cuts at: 0 469 3543
Size: 469 3074
Bsu36I CC'TnA_GG
Cuts at: 0 1190 1874 3543
Size: 1190 684 1669
Fragments arranged by size:
1669 1190 684
BtgI C'CryG_G
Cuts at: 0 639 3543
Size: 639 2904
BtsI GCAGTG_nn'
Cuts at: 0 58 2138 2630 3137 3471 3543
Size: 58 2080 492 507 334 72
Fragments arranged by size:
2080 507 492 334 72 58
DrdI GACnn_nn'nnGTC
Cuts at: 0 505 1055 3543
Size: 505 550 2488
Fragments arranged by size:
2488 550 505
EarI CTCTTCn'nnn_
Cuts at: 0 1773 2123 2258 2861 3161 3543
Size: 1773 350 135 603 300 382
Fragments arranged by size:
1773 603 382 350 300 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 626 2104 2129 2255 2335 3166 3543
Size: 481 145 1478 25 126 80 831 377
Fragments arranged by size:
1478 831 481 377 145 126 80 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3490 3543
Size: 3490 53
EcoO109I rG'GnC_Cy
Cuts at: 0 159 3543
Size: 159 3384
EcoRI G'AATT_C
Cuts at: 0 626 3543
Size: 626 2917
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 529 2134 2906 3543
Size: 529 1605 772 637
Fragments arranged by size:
1605 772 637 529
FspI TGC'GCA
Cuts at: 0 3222 3543
Size: 3222 321
HaeII r_GCGC'y
Cuts at: 0 2100 2199 3543
Size: 2100 99 1344
Fragments arranged by size:
2100 1344 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 318 350 371 403 1542 1572 1574 1604
Size: 318 32 21 32 1139 30 2 30
Cuts at: 1604 1990 1992 2022 2024 3189 3221 3543
Size: 386 2 30 2 1165 32 322
Fragments arranged by size:
1165 1139 386 322 318 32 32 32
30 30 30 21 2 2 2
HincII GTy'rAC
Cuts at: 0 257 2650 3543
Size: 257 2393 893
Fragments arranged by size:
2393 893 257
HindIII A'AGCT_T
Cuts at: 0 3257 3543
Size: 3257 286
KasI G'GCGC_C
Cuts at: 0 2096 3543
Size: 2096 1447
KpnI G_GTAC'C
Cuts at: 0 639 3543
Size: 639 2904
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1734 2022 2586 3543
Size: 1734 288 564 957
Fragments arranged by size:
1734 957 564 288
MslI CAynn'nnrTG
Cuts at: 0 725 757 1445 3543
Size: 725 32 688 2098
Fragments arranged by size:
2098 725 688 32
NarI GG'CG_CC
Cuts at: 0 2097 3543
Size: 2097 1446
NcoI C'CATG_G
Cuts at: 0 639 3543
Size: 639 2904
NheI G'CTAG_C
Cuts at: 0 3210 3543
Size: 3210 333
NsiI A_TGCA'T
Cuts at: 0 1041 2058 2763 3069 3543
Size: 1041 1017 705 306 474
Fragments arranged by size:
1041 1017 705 474 306
NspI r_CATG'y
Cuts at: 0 92 762 3227 3419 3543
Size: 92 670 2465 192 124
Fragments arranged by size:
2465 670 192 124 92
PfoI T'CCnGG_A
Cuts at: 0 61 903 2393 3543
Size: 61 842 1490 1150
Fragments arranged by size:
1490 1150 842 61
PsiI TTA'TAA
Cuts at: 0 1004 3543
Size: 1004 2539
PspXI vC'TCGA_Gb
Cuts at: 0 1721 3543
Size: 1721 1822
PstI C_TGCA'G
Cuts at: 0 53 2145 3543
Size: 53 2092 1398
Fragments arranged by size:
2092 1398 53
SapI GCTCTTCn'nnn_
Cuts at: 0 2123 3161 3543
Size: 2123 1038 382
Fragments arranged by size:
2123 1038 382
ScaI AGT'ACT
Cuts at: 0 2348 3543
Size: 2348 1195
SfcI C'TryA_G
Cuts at: 0 49 1028 2141 3507 3543
Size: 49 979 1113 1366 36
Fragments arranged by size:
1366 1113 979 49 36
SfoI GGC'GCC
Cuts at: 0 2098 3543
Size: 2098 1445
SmlI C'TyrA_G
Cuts at: 0 1721 2284 3543
Size: 1721 563 1259
Fragments arranged by size:
1721 1259 563
SnaBI TAC'GTA
Cuts at: 0 765 3543
Size: 765 2778
SpeI A'CTAG_T
Cuts at: 0 1233 3543
Size: 1233 2310
SphI G_CATG'C
Cuts at: 0 92 3227 3543
Size: 92 3135 316
Fragments arranged by size:
3135 316 92
SspI AAT'ATT
Cuts at: 0 1066 1618 2464 3543
Size: 1066 552 846 1079
Fragments arranged by size:
1079 1066 846 552
StyI C'CwwG_G
Cuts at: 0 639 1262 2175 3008 3543
Size: 639 623 913 833 535
Fragments arranged by size:
913 833 639 623 535
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1994 3543
Size: 1994 1549
TatI w'GTAC_w
Cuts at: 0 1230 2346 2457 2469 2913 3543
Size: 1230 1116 111 12 444 630
Fragments arranged by size:
1230 1116 630 444 111 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1497 1529 1783 1815 3450 3482 3543
Size: 1497 32 254 32 1635 32 61
Fragments arranged by size:
1635 1497 254 61 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3006 3543
Size: 3006 537
XhoI C'TCGA_G
Cuts at: 0 1721 2284 3543
Size: 1721 563 1259
Fragments arranged by size:
1721 1259 563
XmnI GAAnn'nnTTC
Cuts at: 0 1883 2988 3032 3543
Size: 1883 1105 44 511
Fragments arranged by size:
1883 1105 511 44
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AloI AlwNI
ApoI AseI AvaI BaeI BamHI BanI BbeI BbsI
BciVI BclI BfrBI BglII BlpI BmrI BmtI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI
BsrGI BstAPI BstYI BstZ17I Bsu36I BtgI BtsI DrdI
EarI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MmeI MslI NarI
NcoI NheI NsiI NspI PfoI PsiI PspXI PstI
SapI ScaI SfcI SfoI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI ApaI
ApaLI AscI AsiSI AvrII BanII BbvCI BcgI BglI
Bme1580I BmgBI BpuEI BsaWI BsiEI BsiWI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI BtgZI
ClaI CspCI DraI DraIII EaeI EagI EciI EcoICRI
EcoRV FseI FspAI HpaI MfeI MluI MscI MspA1I
NaeI NdeI NgoMIV NotI NruI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SgfI SgrAI SmaI SrfI StuI SwaI
Tth111I XbaI XmaI ZraI