Restriction map for
pF1KA1339
(Linear) (Six-base) MAPSORT of: KIAA1339.seq Check: 9775 from: 1 to: 1926
KIAA1339.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 113 1011 1204 1583 1926
Size: 113 898 193 379 343
Fragments arranged by size:
898 379 343 193 113
AatII G_ACGT'C
Cuts at: 0 933 1926
Size: 933 993
AccI GT'mk_AC
Cuts at: 0 907 1926
Size: 907 1019
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 818 971 1374 1599 1892 1926
Size: 818 153 403 225 293 34
Fragments arranged by size:
818 403 293 225 153 34
AfeI AGC'GCT
Cuts at: 0 1303 1926
Size: 1303 623
AflIII A'CryG_T
Cuts at: 0 752 1064 1926
Size: 752 312 862
Fragments arranged by size:
862 752 312
AleI CACnn'nnGTG
Cuts at: 0 1040 1926
Size: 1040 886
AlwNI CAG_nnn'CTG
Cuts at: 0 940 1926
Size: 940 986
ApaI G_GGCC'C
Cuts at: 0 729 1926
Size: 729 1197
BamHI G'GATC_C
Cuts at: 0 377 1926
Size: 377 1549
BanI G'GyrC_C
Cuts at: 0 420 1926
Size: 420 1506
BanII G_rGCy'C
Cuts at: 0 729 1344 1926
Size: 729 615 582
Fragments arranged by size:
729 615 582
BbvCI CC'TCA_GC
Cuts at: 0 5 1153 1926
Size: 5 1148 773
Fragments arranged by size:
1148 773 5
BglI GCCn_nnn'nGGC
Cuts at: 0 151 1926
Size: 151 1775
Bme1580I G_kGCm'C
Cuts at: 0 729 826 1119 1126 1374 1926
Size: 729 97 293 7 248 552
Fragments arranged by size:
729 552 293 248 97 7
BmgBI CAC'GTC
Cuts at: 0 753 1926
Size: 753 1173
BmrI ACTGGGnnnn_n'
Cuts at: 0 647 1113 1368 1847 1926
Size: 647 466 255 479 79
Fragments arranged by size:
647 479 466 255 79
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 358 377 446 992 1926
Size: 295 63 19 69 546 934
Fragments arranged by size:
934 546 295 69 63 19
Bpu10I CC'TnA_GC
Cuts at: 0 5 1127 1153 1926
Size: 5 1122 26 773
Fragments arranged by size:
1122 773 26 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1729 1926
Size: 1729 197
BsaI GGTCTCn'nnnn_
Cuts at: 0 1876 1926
Size: 1876 50
BsaHI Gr'CG_yC
Cuts at: 0 930 1926
Size: 930 996
BsaWI w'CCGG_w
Cuts at: 0 732 1334 1926
Size: 732 602 592
Fragments arranged by size:
732 602 592
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 268 298 1690 1720 1904 1926
Size: 268 30 1392 30 184 22
Fragments arranged by size:
1392 268 184 30 30 22
BseYI C'CCAG_C
Cuts at: 0 84 294 1926
Size: 84 210 1632
Fragments arranged by size:
1632 210 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1521 1579 1926
Size: 169 1352 58 347
Fragments arranged by size:
1352 347 169 58
BsiHKAI G_wGCw'C
Cuts at: 0 1103 1344 1574 1926
Size: 1103 241 230 352
Fragments arranged by size:
1103 352 241 230
Bsp1286I G_dGCh'C
Cuts at: 0 729 826 1103 1119 1126 1344 1374 1574
Size: 729 97 277 16 7 218 30 200
Cuts at: 1574 1926
Size: 352
Fragments arranged by size:
729 352 277 218 200 97 30 16
7
BspHI T'CATG_A
Cuts at: 0 512 1926
Size: 512 1414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 113 362 953 1011 1204 1583 1926
Size: 113 249 591 58 193 379 343
Fragments arranged by size:
591 379 343 249 193 113 58
BsrBI CCG'CTC
Cuts at: 0 1608 1692 1926
Size: 1608 84 234
Fragments arranged by size:
1608 234 84
BsrFI r'CCGG_y
Cuts at: 0 182 1586 1926
Size: 182 1404 340
Fragments arranged by size:
1404 340 182
BsrGI T'GTAC_A
Cuts at: 0 755 1926
Size: 755 1171
BstAPI GCAn_nnn'nTGC
Cuts at: 0 871 1544 1926
Size: 871 673 382
Fragments arranged by size:
871 673 382
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1042 1897 1926
Size: 1042 855 29
Fragments arranged by size:
1042 855 29
BstYI r'GATC_y
Cuts at: 0 173 377 811 1926
Size: 173 204 434 1115
Fragments arranged by size:
1115 434 204 173
Bsu36I CC'TnA_GG
Cuts at: 0 716 827 1926
Size: 716 111 1099
Fragments arranged by size:
1099 716 111
BtgI C'CryG_G
Cuts at: 0 542 1926
Size: 542 1384
DraIII CAC_nnn'GTG
Cuts at: 0 1207 1926
Size: 1207 719
EaeI y'GGCC_r
Cuts at: 0 140 1479 1926
Size: 140 1339 447
Fragments arranged by size:
1339 447 140
EarI CTCTTCn'nnn_
Cuts at: 0 77 653 704 773 852 1926
Size: 77 576 51 69 79 1074
Fragments arranged by size:
1074 576 79 77 69 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1162 1486 1926
Size: 1162 324 440
Fragments arranged by size:
1162 440 324
EcoICRI GAG'CTC
Cuts at: 0 1342 1926
Size: 1342 584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 358 377 446 818 971 992 1374
Size: 295 63 19 69 372 153 21 382
Cuts at: 1374 1599 1892 1926
Size: 225 293 34
Fragments arranged by size:
382 372 295 293 225 153 69 63
34 21 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 307 1926
Size: 307 1619
EcoO109I rG'GnC_Cy
Cuts at: 0 725 726 1358 1825 1926
Size: 725 1 632 467 101
Fragments arranged by size:
725 632 467 101 1
EcoRV GAT'ATC
Cuts at: 0 411 1926
Size: 411 1515
FspI TGC'GCA
Cuts at: 0 361 1564 1926
Size: 361 1203 362
Fragments arranged by size:
1203 362 361
HaeII r_GCGC'y
Cuts at: 0 1305 1443 1926
Size: 1305 138 483
Fragments arranged by size:
1305 483 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 427 459 559 591 673 705 963 995
Size: 427 32 100 32 82 32 258 32
Cuts at: 995 1206 1238 1926
Size: 211 32 688
Fragments arranged by size:
688 427 258 211 100 82 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1866 1926
Size: 1866 60
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1926
Size: 187 1739
MslI CAynn'nnrTG
Cuts at: 0 30 541 1040 1926
Size: 30 511 499 886
Fragments arranged by size:
886 511 499 30
MspA1I CmG'CkG
Cuts at: 0 239 273 1367 1422 1926
Size: 239 34 1094 55 504
Fragments arranged by size:
1094 504 239 55 34
NaeI GCC'GGC
Cuts at: 0 184 1926
Size: 184 1742
NcoI C'CATG_G
Cuts at: 0 542 1926
Size: 542 1384
NgoMIV G'CCGG_C
Cuts at: 0 182 1926
Size: 182 1744
NspI r_CATG'y
Cuts at: 0 1068 1926
Size: 1068 858
PciI A'CATG_T
Cuts at: 0 1064 1926
Size: 1064 862
PflMI CCAn_nnn'nTGG
Cuts at: 0 1892 1926
Size: 1892 34
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1009 1041 1926
Size: 1009 32 885
Fragments arranged by size:
1009 885 32
PpuMI rG'GwC_Cy
Cuts at: 0 1825 1926
Size: 1825 101
PspOMI G'GGCC_C
Cuts at: 0 725 1926
Size: 725 1201
PstI C_TGCA'G
Cuts at: 0 62 110 311 329 1549 1926
Size: 62 48 201 18 1220 377
Fragments arranged by size:
1220 377 201 62 48 18
PvuII CAG'CTG
Cuts at: 0 239 273 1926
Size: 239 34 1653
Fragments arranged by size:
1653 239 34
SacI G_AGCT'C
Cuts at: 0 1344 1926
Size: 1344 582
SapI GCTCTTCn'nnn_
Cuts at: 0 653 704 773 1926
Size: 653 51 69 1153
Fragments arranged by size:
1153 653 69 51
SexAI A'CCwGG_T
Cuts at: 0 1821 1926
Size: 1821 105
SfcI C'TryA_G
Cuts at: 0 58 106 155 307 325 1545 1926
Size: 58 48 49 152 18 1220 381
Fragments arranged by size:
1220 381 152 58 49 48 18
SgrAI Cr'CCGG_yG
Cuts at: 0 1586 1926
Size: 1586 340
SmlI C'TyrA_G
Cuts at: 0 1744 1926
Size: 1744 182
StuI AGG'CCT
Cuts at: 0 803 1926
Size: 803 1123
StyI C'CwwG_G
Cuts at: 0 542 740 1926
Size: 542 198 1186
Fragments arranged by size:
1186 542 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1241 1827 1849 1926
Size: 1241 586 22 77
Fragments arranged by size:
1241 586 77 22
TatI w'GTAC_w
Cuts at: 0 755 1926
Size: 755 1171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 28 60 268 300 1257 1289 1926
Size: 28 32 208 32 957 32 637
Fragments arranged by size:
957 637 208 32 32 32 28
XbaI T'CTAG_A
Cuts at: 0 1878 1926
Size: 1878 48
ZraI GAC'GTC
Cuts at: 0 931 1926
Size: 931 995
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AleI AlwNI
ApaI BamHI BanI BanII BbvCI BglI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI
BsrGI BstAPI BstXI BstYI Bsu36I BtgI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI
HaeII Hin4I HincII MmeI MslI MspA1I NaeI NcoI
NgoMIV NspI PciI PflMI PpiI PpuMI PspOMI PstI
PvuII SacI SapI SexAI SfcI SgrAI SmlI StuI
StyI TaqII TatI TstI XbaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AlfI AloI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI BmtI
BplI BsaAI BsaBI BseRI BsiEI BsiWI BsmI BsmBI
BspEI BsrDI BssHII BssSI BstBI BstEII BstZ17I BtgZI
BtsI ClaI CspCI DraI DrdI EagI EcoRI FalI
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
MscI NarI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XcmI XhoI XmaI XmnI