Restriction map for
pF1KA1342
(Linear) (Six-base) MAPSORT of: KIAA1342.seq Check: 7896 from: 1 to: 1275
KIAA1342.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 422 821 839 1275
Size: 45 377 399 18 436
Fragments arranged by size:
436 399 377 45 18
AflII C'TTAA_G
Cuts at: 0 169 1275
Size: 169 1106
AleI CACnn'nnGTG
Cuts at: 0 49 1275
Size: 49 1226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 123 1275
Size: 123 1152
ApoI r'AATT_y
Cuts at: 0 28 267 1117 1275
Size: 28 239 850 158
Fragments arranged by size:
850 239 158 28
AvaI C'yCGr_G
Cuts at: 0 783 1275
Size: 783 492
BamHI G'GATC_C
Cuts at: 0 645 1275
Size: 645 630
BbsI GAAGACnn'nnnn_
Cuts at: 0 72 845 1030 1275
Size: 72 773 185 245
Fragments arranged by size:
773 245 185 72
BciVI GTATCCnnnnn_n'
Cuts at: 0 674 1275
Size: 674 601
BglII A'GATC_T
Cuts at: 0 297 1220 1275
Size: 297 923 55
Fragments arranged by size:
923 297 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1275
Size: 373 902
BsaI GGTCTCn'nnnn_
Cuts at: 0 655 1275
Size: 655 620
BsaAI yAC'GTr
Cuts at: 0 1260 1275
Size: 1260 15
BsaWI w'CCGG_w
Cuts at: 0 958 1275
Size: 958 317
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 134 1275
Size: 134 1141
BseYI C'CCAG_C
Cuts at: 0 459 1275
Size: 459 816
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1202 1275
Size: 1202 73
BsiHKAI G_wGCw'C
Cuts at: 0 1211 1265 1275
Size: 1211 54 10
Fragments arranged by size:
1211 54 10
BsmI GAATG_Cn'
Cuts at: 0 363 1275
Size: 363 912
Bsp1286I G_dGCh'C
Cuts at: 0 1211 1265 1275
Size: 1211 54 10
Fragments arranged by size:
1211 54 10
BspEI T'CCGG_A
Cuts at: 0 958 1275
Size: 958 317
BsrDI GCAATG_nn'
Cuts at: 0 271 1275
Size: 271 1004
BstXI CCAn_nnnn'nTGG
Cuts at: 0 51 1275
Size: 51 1224
BstYI r'GATC_y
Cuts at: 0 56 104 282 297 645 969 1220 1275
Size: 56 48 178 15 348 324 251 55
Fragments arranged by size:
348 324 251 178 56 55 48 15
BtgI C'CryG_G
Cuts at: 0 531 545 1275
Size: 531 14 730
Fragments arranged by size:
730 531 14
BtsI GCAGTG_nn'
Cuts at: 0 1061 1275
Size: 1061 214
DraI TTT'AAA
Cuts at: 0 416 1275
Size: 416 859
EarI CTCTTCn'nnn_
Cuts at: 0 437 475 1275
Size: 437 38 800
Fragments arranged by size:
800 437 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 373 422 821 839 1275
Size: 45 328 49 399 18 436
Fragments arranged by size:
436 399 328 49 45 18
EcoO109I rG'GnC_Cy
Cuts at: 0 1095 1145 1275
Size: 1095 50 130
Fragments arranged by size:
1095 130 50
EcoRI G'AATT_C
Cuts at: 0 267 1275
Size: 267 1008
EcoRV GAT'ATC
Cuts at: 0 756 1275
Size: 756 519
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 96 128 541 573 740 772 815 847
Size: 96 32 413 32 167 32 43 32
Cuts at: 847 1275
Size: 428
Fragments arranged by size:
428 413 167 96 43 32 32 32
32
MslI CAynn'nnrTG
Cuts at: 0 49 1275
Size: 49 1226
MspA1I CmG'CkG
Cuts at: 0 256 1275
Size: 256 1019
NcoI C'CATG_G
Cuts at: 0 545 1275
Size: 545 730
NspI r_CATG'y
Cuts at: 0 166 1275
Size: 166 1109
PflMI CCAn_nnn'nTGG
Cuts at: 0 705 1275
Size: 705 570
PmlI CAC'GTG
Cuts at: 0 1260 1275
Size: 1260 15
PpuMI rG'GwC_Cy
Cuts at: 0 1145 1275
Size: 1145 130
PvuII CAG'CTG
Cuts at: 0 256 1275
Size: 256 1019
SanDI GG'GwC_CC
Cuts at: 0 1145 1275
Size: 1145 130
SmlI C'TyrA_G
Cuts at: 0 169 1275
Size: 169 1106
StyI C'CwwG_G
Cuts at: 0 545 641 1275
Size: 545 96 634
Fragments arranged by size:
634 545 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 706 1168 1275
Size: 706 462 107
Fragments arranged by size:
706 462 107
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 48 80 1275
Size: 48 32 1195
Fragments arranged by size:
1195 48 32
Enzymes that do cut:
AcuI AflII AleI AlfI ApoI AvaI BamHI BbsI
BciVI BglII BpmI BsaI BsaAI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BsrDI BstXI BstYI
BtgI BtsI DraI EarI Eco57MI EcoO109I EcoRI EcoRV
Hin4I MslI MspA1I NcoI NspI PflMI PmlI PpuMI
PvuII SanDI SmlI StyI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BanII BbeI BbvCI BcgI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaBI BsaHI BsaXI BsiEI BsiWI BsmBI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI