(Linear) (Six-base) MAPSORT of: KIAA1343.seq Check: 2742 from: 1 to: 1485
KIAA1343.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AgeI A'CCGG_T
Cuts at: 0 1407 1485
Size: 1407 78
AhdI GACnn_n'nnGTC
Cuts at: 0 1172 1485
Size: 1172 313
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 548 580 1485
Size: 548 32 905
Fragments arranged by size:
905 548 32
ApaLI G'TGCA_C
Cuts at: 0 791 1485
Size: 791 694
ApoI r'AATT_y
Cuts at: 0 37 826 1454 1485
Size: 37 789 628 31
Fragments arranged by size:
789 628 37 31
AseI AT'TA_AT
Cuts at: 0 857 1485
Size: 857 628
BamHI G'GATC_C
Cuts at: 0 29 1485
Size: 29 1456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1297 1331 1485
Size: 1297 34 154
Fragments arranged by size:
1297 154 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 121 1485
Size: 121 1364
BlpI GC'TnA_GC
Cuts at: 0 776 1485
Size: 776 709
Bme1580I G_kGCm'C
Cuts at: 0 795 1485
Size: 795 690
BmrI ACTGGGnnnn_n'
Cuts at: 0 1180 1485
Size: 1180 305
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 765 1485
Size: 765 720
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1288 1485
Size: 1288 197
BsaI GGTCTCn'nnnn_
Cuts at: 0 101 1485
Size: 101 1384
BsaBI GATnn'nnATC
Cuts at: 0 518 1485
Size: 518 967
BsaHI Gr'CG_yC
Cuts at: 0 996 1485
Size: 996 489
BsaWI w'CCGG_w
Cuts at: 0 255 1407 1485
Size: 255 1152 78
Fragments arranged by size:
1152 255 78
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 892 1027 1099 1151 1153 1286 1485
Size: 892 135 72 52 2 133 199
Fragments arranged by size:
892 199 135 133 72 52 2
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1465 1485
Size: 1465 20
BsiHKAI G_wGCw'C
Cuts at: 0 795 1126 1485
Size: 795 331 359
Fragments arranged by size:
795 359 331
BsmI GAATG_Cn'
Cuts at: 0 777 1485
Size: 777 708
Bsp1286I G_dGCh'C
Cuts at: 0 795 1126 1485
Size: 795 331 359
Fragments arranged by size:
795 359 331
BspEI T'CCGG_A
Cuts at: 0 255 1485
Size: 255 1230
BsrDI GCAATG_nn'
Cuts at: 0 137 1485
Size: 137 1348
BsrFI r'CCGG_y
Cuts at: 0 1286 1407 1485
Size: 1286 121 78
Fragments arranged by size:
1286 121 78
BstYI r'GATC_y
Cuts at: 0 29 1485
Size: 29 1456
Bsu36I CC'TnA_GG
Cuts at: 0 653 724 1485
Size: 653 71 761
Fragments arranged by size:
761 653 71
BtgI C'CryG_G
Cuts at: 0 1418 1485
Size: 1418 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 601 1485
Size: 601 884
BtsI GCAGTG_nn'
Cuts at: 0 899 1261 1477 1485
Size: 899 362 216 8
Fragments arranged by size:
899 362 216 8
DraI TTT'AAA
Cuts at: 0 1343 1485
Size: 1343 142
EaeI y'GGCC_r
Cuts at: 0 963 1333 1485
Size: 963 370 152
Fragments arranged by size:
963 370 152
EarI CTCTTCn'nnn_
Cuts at: 0 588 1114 1130 1286 1485
Size: 588 526 16 156 199
Fragments arranged by size:
588 526 199 156 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1288 1485
Size: 1288 197
EcoRI G'AATT_C
Cuts at: 0 826 1454 1485
Size: 826 628 31
Fragments arranged by size:
826 628 31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 996 1485
Size: 996 489
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1158 1190 1456 1485
Size: 1158 32 266 29
Fragments arranged by size:
1158 266 32 29
HincII GTy'rAC
Cuts at: 0 1432 1485
Size: 1432 53
MfeI C'AATT_G
Cuts at: 0 143 356 1485
Size: 143 213 1129
Fragments arranged by size:
1129 213 143
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 1228 1282 1485
Size: 847 381 54 203
Fragments arranged by size:
847 381 203 54
MscI TGG'CCA
Cuts at: 0 965 1485
Size: 965 520
NspI r_CATG'y
Cuts at: 0 192 663 1485
Size: 192 471 822
Fragments arranged by size:
822 471 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 499 1485
Size: 499 986
SapI GCTCTTCn'nnn_
Cuts at: 0 1114 1286 1485
Size: 1114 172 199
Fragments arranged by size:
1114 199 172
SfcI C'TryA_G
Cuts at: 0 696 990 1485
Size: 696 294 495
Fragments arranged by size:
696 495 294
SspI AAT'ATT
Cuts at: 0 376 1485
Size: 376 1109
TatI w'GTAC_w
Cuts at: 0 59 1485
Size: 59 1426
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 237 269 495 527 1485
Size: 237 32 226 32 958
Fragments arranged by size:
958 237 226 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1057 1485
Size: 1057 428
XmnI GAAnn'nnTTC
Cuts at: 0 976 1485
Size: 976 509
Enzymes that do cut:
AgeI AhdI AloI ApaLI ApoI AseI BamHI BcgI
BciVI BlpI Bme1580I BmrI BplI BpmI BsaI BsaBI
BsaHI BsaWI BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI
BsrDI BsrFI BstYI Bsu36I BtgI BtgZI BtsI DraI
EaeI EarI Eco57MI EcoRI FalI Hin4I HincII MfeI
MmeI MscI NspI PflMI SapI SfcI SspI TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AleI AlfI AlwNI ApaI AscI AsiSI AvaI
AvrII BaeI BanI BanII BbeI BbsI BbvCI BclI
BfrBI BglI BglII BmgBI BmtI Bpu10I BpuEI BsaAI
BsaXI BseYI BsiEI BsiWI BsmBI BspHI BspMI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MluI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
StuI StyI SwaI TaqII Tth111I XbaI XhoI XmaI
ZraI