Restriction map for
pF1KSDA1345
(Linear) (Six-base) MAPSORT of: KIAA1345.seq Check: 1605 from: 1 to: 4536
KIAA1345.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 552 4536
Size: 552 3984
AccI GT'mk_AC
Cuts at: 0 3623 4466 4536
Size: 3623 843 70
Fragments arranged by size:
3623 843 70
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2014 2150 3224 4136 4403 4536
Size: 2014 136 1074 912 267 133
Fragments arranged by size:
2014 1074 912 267 136 133
AfeI AGC'GCT
Cuts at: 0 1891 4135 4536
Size: 1891 2244 401
Fragments arranged by size:
2244 1891 401
AflII C'TTAA_G
Cuts at: 0 1369 4536
Size: 1369 3167
AflIII A'CryG_T
Cuts at: 0 3577 4536
Size: 3577 959
AhdI GACnn_n'nnGTC
Cuts at: 0 1943 4536
Size: 1943 2593
ApoI r'AATT_y
Cuts at: 0 142 277 1059 1195 1313 1330 1505 2620
Size: 142 135 782 136 118 17 175 1115
Cuts at: 2620 3037 4032 4450 4536
Size: 417 995 418 86
Fragments arranged by size:
1115 995 782 418 417 175 142 136
135 118 86 17
AvaI C'yCGr_G
Cuts at: 0 1687 1866 4536
Size: 1687 179 2670
Fragments arranged by size:
2670 1687 179
BamHI G'GATC_C
Cuts at: 0 899 4536
Size: 899 3637
BanI G'GyrC_C
Cuts at: 0 2613 4536
Size: 2613 1923
BanII G_rGCy'C
Cuts at: 0 56 1724 1796 2124 3255 3614 4536
Size: 56 1668 72 328 1131 359 922
Fragments arranged by size:
1668 1131 922 359 328 72 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 3050 4536
Size: 3050 1486
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3167 3201 4536
Size: 3167 34 1335
Fragments arranged by size:
3167 1335 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1694 2285 4536
Size: 1694 591 2251
Fragments arranged by size:
2251 1694 591
BclI T'GATC_A
Cuts at: 0 1034 3738 4536
Size: 1034 2704 798
Fragments arranged by size:
2704 1034 798
BglI GCCn_nnn'nGGC
Cuts at: 0 1144 2413 4536
Size: 1144 1269 2123
Fragments arranged by size:
2123 1269 1144
BglII A'GATC_T
Cuts at: 0 1039 4536
Size: 1039 3497
BlpI GC'TnA_GC
Cuts at: 0 1813 4157 4536
Size: 1813 2344 379
Fragments arranged by size:
2344 1813 379
Bme1580I G_kGCm'C
Cuts at: 0 1852 2198 4536
Size: 1852 346 2338
Fragments arranged by size:
2338 1852 346
BmrI ACTGGGnnnn_n'
Cuts at: 0 248 713 1143 4536
Size: 248 465 430 3393
Fragments arranged by size:
3393 465 430 248
BmtI G_CTAG'C
Cuts at: 0 2074 4536
Size: 2074 2462
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 122 724 4536
Size: 122 602 3812
Fragments arranged by size:
3812 602 122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 1623 1700 2542 2752 3747 4227 4439
Size: 1064 559 77 842 210 995 480 212
Cuts at: 4439 4536
Size: 97
Fragments arranged by size:
1064 995 842 559 480 212 210 97
77
Bpu10I CC'TnA_GC
Cuts at: 0 2878 4536
Size: 2878 1658
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 4536
Size: 327 4209
BsaI GGTCTCn'nnnn_
Cuts at: 0 1040 1869 4536
Size: 1040 829 2667
Fragments arranged by size:
2667 1040 829
BsaAI yAC'GTr
Cuts at: 0 630 3101 4536
Size: 630 2471 1435
Fragments arranged by size:
2471 1435 630
BsaBI GATnn'nnATC
Cuts at: 0 4014 4536
Size: 4014 522
BsaWI w'CCGG_w
Cuts at: 0 83 4536
Size: 83 4453
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1419 1449 2916 2946 4283 4313 4536
Size: 1419 30 1467 30 1337 30 223
Fragments arranged by size:
1467 1419 1337 223 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 218 529 532 569 1561 1705 1794
Size: 64 154 311 3 37 992 144 89
Cuts at: 1794 3593 4536
Size: 1799 943
Fragments arranged by size:
1799 992 943 311 154 144 89 64
37 3
BseYI C'CCAG_C
Cuts at: 0 1708 3399 4335 4536
Size: 1708 1691 936 201
Fragments arranged by size:
1708 1691 936 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 350 530 4536
Size: 64 286 180 4006
Fragments arranged by size:
4006 286 180 64
BsiHKAI G_wGCw'C
Cuts at: 0 1541 3614 3733 4536
Size: 1541 2073 119 803
Fragments arranged by size:
2073 1541 803 119
Bsp1286I G_dGCh'C
Cuts at: 0 56 1541 1724 1796 1852 2124 2198 3255
Size: 56 1485 183 72 56 272 74 1057
Cuts at: 3255 3614 3733 4536
Size: 359 119 803
Fragments arranged by size:
1485 1057 803 359 272 183 119 74
72 56 56
BspEI T'CCGG_A
Cuts at: 0 83 4536
Size: 83 4453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 552 666 2763 4536
Size: 552 114 2097 1773
Fragments arranged by size:
2097 1773 552 114
BsrBI CCG'CTC
Cuts at: 0 499 1858 4536
Size: 499 1359 2678
Fragments arranged by size:
2678 1359 499
BsrDI GCAATG_nn'
Cuts at: 0 205 1363 3846 4536
Size: 205 1158 2483 690
Fragments arranged by size:
2483 1158 690 205
BsrFI r'CCGG_y
Cuts at: 0 3024 4536
Size: 3024 1512
BsrGI T'GTAC_A
Cuts at: 0 762 4536
Size: 762 3774
BssSI C'ACGA_G
Cuts at: 0 2173 4536
Size: 2173 2363
BstAPI GCAn_nnn'nTGC
Cuts at: 0 202 4536
Size: 202 4334
BstXI CCAn_nnnn'nTGG
Cuts at: 0 777 4536
Size: 777 3759
BstYI r'GATC_y
Cuts at: 0 899 1039 2572 4505 4536
Size: 899 140 1533 1933 31
Fragments arranged by size:
1933 1533 899 140 31
BstZ17I GTA'TAC
Cuts at: 0 4467 4536
Size: 4467 69
Bsu36I CC'TnA_GG
Cuts at: 0 2244 3605 3851 4536
Size: 2244 1361 246 685
Fragments arranged by size:
2244 1361 685 246
BtgI C'CryG_G
Cuts at: 0 94 4536
Size: 94 4442
BtsI GCAGTG_nn'
Cuts at: 0 1742 2911 3035 3921 4536
Size: 1742 1169 124 886 615
Fragments arranged by size:
1742 1169 886 615 124
DraI TTT'AAA
Cuts at: 0 3596 4536
Size: 3596 940
DrdI GACnn_nn'nnGTC
Cuts at: 0 2057 4536
Size: 2057 2479
EaeI y'GGCC_r
Cuts at: 0 1948 2011 2405 2917 3135 4536
Size: 1948 63 394 512 218 1401
Fragments arranged by size:
1948 1401 512 394 218 63
EarI CTCTTCn'nnn_
Cuts at: 0 413 715 1529 4112 4370 4536
Size: 413 302 814 2583 258 166
Fragments arranged by size:
2583 814 413 302 258 166
EcoICRI GAG'CTC
Cuts at: 0 3612 4536
Size: 3612 924
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 1623 1700 2014 2150 2542 2752 3224
Size: 1064 559 77 314 136 392 210 472
Cuts at: 3224 3747 4136 4227 4403 4439 4536
Size: 523 389 91 176 36 97
Fragments arranged by size:
1064 559 523 472 392 389 314 210
176 136 97 91 77 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 548 2770 3136 4536
Size: 548 2222 366 1400
Fragments arranged by size:
2222 1400 548 366
EcoO109I rG'GnC_Cy
Cuts at: 0 864 1695 3979 4536
Size: 864 831 2284 557
Fragments arranged by size:
2284 864 831 557
EcoRI G'AATT_C
Cuts at: 0 2620 4536
Size: 2620 1916
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 601 4536
Size: 601 3935
HaeII r_GCGC'y
Cuts at: 0 1893 4137 4536
Size: 1893 2244 399
Fragments arranged by size:
2244 1893 399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 891 923 1291 1323 1387 1419 1847 1879
Size: 891 32 368 32 64 32 428 32
Cuts at: 1879 1929 1961 2347 2379 2916 2948 3685 3717
Size: 50 32 386 32 537 32 737 32
Cuts at: 3717 4536
Size: 819
Fragments arranged by size:
891 819 737 537 428 386 368 64
50 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 327 2007 4536
Size: 327 1680 2529
Fragments arranged by size:
2529 1680 327
HindIII A'AGCT_T
Cuts at: 0 748 1217 4536
Size: 748 469 3319
Fragments arranged by size:
3319 748 469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1089 2028 2163 2914 3883 4536
Size: 1089 939 135 751 969 653
Fragments arranged by size:
1089 969 939 751 653 135
MscI TGG'CCA
Cuts at: 0 2407 4536
Size: 2407 2129
MslI CAynn'nnrTG
Cuts at: 0 1661 3013 3926 4536
Size: 1661 1352 913 610
Fragments arranged by size:
1661 1352 913 610
MspA1I CmG'CkG
Cuts at: 0 2229 2529 4150 4156 4335 4536
Size: 2229 300 1621 6 179 201
Fragments arranged by size:
2229 1621 300 201 179 6
NaeI GCC'GGC
Cuts at: 0 3026 4536
Size: 3026 1510
NdeI CA'TA_TG
Cuts at: 0 1494 4536
Size: 1494 3042
NgoMIV G'CCGG_C
Cuts at: 0 3024 4536
Size: 3024 1512
NheI G'CTAG_C
Cuts at: 0 2070 4536
Size: 2070 2466
NspI r_CATG'y
Cuts at: 0 1033 1129 2161 3085 3781 4374 4536
Size: 1033 96 1032 924 696 593 162
Fragments arranged by size:
1033 1032 924 696 593 162 96
PflMI CCAn_nnn'nTGG
Cuts at: 0 4058 4093 4536
Size: 4058 35 443
Fragments arranged by size:
4058 443 35
PfoI T'CCnGG_A
Cuts at: 0 776 1053 1805 1933 2678 4536
Size: 776 277 752 128 745 1858
Fragments arranged by size:
1858 776 752 745 277 128
PmlI CAC'GTG
Cuts at: 0 630 4536
Size: 630 3906
PpuMI rG'GwC_Cy
Cuts at: 0 864 1695 3979 4536
Size: 864 831 2284 557
Fragments arranged by size:
2284 864 831 557
PsiI TTA'TAA
Cuts at: 0 2633 4536
Size: 2633 1903
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 998 1030 3767 3799 4536
Size: 998 32 2737 32 737
Fragments arranged by size:
2737 998 737 32 32
PstI C_TGCA'G
Cuts at: 0 215 417 663 692 3287 3651 3916 4155
Size: 215 202 246 29 2595 364 265 239
Cuts at: 4155 4536
Size: 381
Fragments arranged by size:
2595 381 364 265 246 239 215 202
29
PvuII CAG'CTG
Cuts at: 0 2229 2529 4150 4156 4335 4536
Size: 2229 300 1621 6 179 201
Fragments arranged by size:
2229 1621 300 201 179 6
SacI G_AGCT'C
Cuts at: 0 3614 4536
Size: 3614 922
SapI GCTCTTCn'nnn_
Cuts at: 0 715 1529 4112 4536
Size: 715 814 2583 424
Fragments arranged by size:
2583 814 715 424
ScaI AGT'ACT
Cuts at: 0 950 3329 3785 4416 4536
Size: 950 2379 456 631 120
Fragments arranged by size:
2379 950 631 456 120
SfcI C'TryA_G
Cuts at: 0 211 413 659 688 3283 3637 3647 3912
Size: 211 202 246 29 2595 354 10 265
Cuts at: 3912 4151 4344 4536
Size: 239 193 192
Fragments arranged by size:
2595 354 265 246 239 211 202 193
192 29 10
SmlI C'TyrA_G
Cuts at: 0 342 1369 1866 4536
Size: 342 1027 497 2670
Fragments arranged by size:
2670 1027 497 342
SphI G_CATG'C
Cuts at: 0 3085 4536
Size: 3085 1451
SspI AAT'ATT
Cuts at: 0 2818 3990 4002 4431 4536
Size: 2818 1172 12 429 105
Fragments arranged by size:
2818 1172 429 105 12
StuI AGG'CCT
Cuts at: 0 424 791 1600 1785 3823 4536
Size: 424 367 809 185 2038 713
Fragments arranged by size:
2038 809 713 424 367 185
StyI C'CwwG_G
Cuts at: 0 21 1786 2723 2920 4536
Size: 21 1765 937 197 1616
Fragments arranged by size:
1765 1616 937 197 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1851 2669 2755 4490 4536
Size: 1851 818 86 1735 46
Fragments arranged by size:
1851 1735 818 86 46
TatI w'GTAC_w
Cuts at: 0 762 948 1898 3327 3783 4414 4422 4536
Size: 762 186 950 1429 456 631 8 114
Fragments arranged by size:
1429 950 762 631 456 186 114 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 389 421 1089 1121 2177 2209 3057 3089
Size: 389 32 668 32 1056 32 848 32
Cuts at: 3089 4536
Size: 1447
Fragments arranged by size:
1447 1056 848 668 389 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3697 4536
Size: 3697 839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 397 2179 3218 4536
Size: 397 1782 1039 1318
Fragments arranged by size:
1782 1318 1039 397
XhoI C'TCGA_G
Cuts at: 0 1866 4536
Size: 1866 2670
XmnI GAAnn'nnTTC
Cuts at: 0 2081 2852 4066 4536
Size: 2081 771 1214 470
Fragments arranged by size:
2081 1214 771 470
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AhdI ApoI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstXI BstYI BstZ17I Bsu36I
BtgI BtsI DraI DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
MmeI MscI MslI MspA1I NaeI NdeI NgoMIV NheI
NspI PflMI PfoI PmlI PpuMI PsiI PsrI PstI
PvuII SacI SapI ScaI SfcI SmlI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AleI AlfI AloI AlwNI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BfrBI BmgBI BsaHI BsiEI BsiWI BsmI BsmBI
BspHI BssHII BstBI BstEII BtgZI ClaI CspCI DraIII
EagI EciI EcoRV FseI FspI FspAI HpaI KasI
KpnI MfeI MluI NarI NcoI NotI NruI NsiI
PacI PasI PciI PmeI PpiI PshAI PspOMI PspXI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI
XbaI XmaI ZraI