Restriction map for
pF1KSDA1350
(Linear) (Six-base) MAPSORT of: KIAA1350.seq Check: 8695 from: 1 to: 2634
KIAA1350.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AccI GT'mk_AC
Cuts at: 0 1809 2634
Size: 1809 825
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1569 1627 2297 2634
Size: 1569 58 670 337
Fragments arranged by size:
1569 670 337 58
AflIII A'CryG_T
Cuts at: 0 1767 2634
Size: 1767 867
AleI CACnn'nnGTG
Cuts at: 0 683 2634
Size: 683 1951
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2083 2115 2634
Size: 2083 32 519
Fragments arranged by size:
2083 519 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2041 2634
Size: 2041 593
ApoI r'AATT_y
Cuts at: 0 113 634 1686 1714 1891 1917 2634
Size: 113 521 1052 28 177 26 717
Fragments arranged by size:
1052 717 521 177 113 28 26
BamHI G'GATC_C
Cuts at: 0 1278 2634
Size: 1278 1356
BanI G'GyrC_C
Cuts at: 0 2449 2634
Size: 2449 185
BbsI GAAGACnn'nnnn_
Cuts at: 0 869 1784 1997 2634
Size: 869 915 213 637
Fragments arranged by size:
915 869 637 213
BciVI GTATCCnnnnn_n'
Cuts at: 0 2547 2634
Size: 2547 87
BclI T'GATC_A
Cuts at: 0 606 720 1488 2634
Size: 606 114 768 1146
Fragments arranged by size:
1146 768 606 114
BfrBI ATG'CAT
Cuts at: 0 422 2461 2634
Size: 422 2039 173
Fragments arranged by size:
2039 422 173
BglII A'GATC_T
Cuts at: 0 2131 2634
Size: 2131 503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 2634
Size: 1022 1612
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 660 690 1621 1651 2634
Size: 660 30 931 30 983
Fragments arranged by size:
983 931 660 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2036 2409 2634
Size: 2036 373 225
Fragments arranged by size:
2036 373 225
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 983 2634
Size: 983 1651
BsiHKAI G_wGCw'C
Cuts at: 0 762 2634
Size: 762 1872
BsmI GAATG_Cn'
Cuts at: 0 177 422 1891 2634
Size: 177 245 1469 743
Fragments arranged by size:
1469 743 245 177
Bsp1286I G_dGCh'C
Cuts at: 0 762 2634
Size: 762 1872
BspHI T'CATG_A
Cuts at: 0 1710 2634
Size: 1710 924
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 495 2634
Size: 495 2139
BsrGI T'GTAC_A
Cuts at: 0 1770 2634
Size: 1770 864
BstAPI GCAn_nnn'nTGC
Cuts at: 0 428 2634
Size: 428 2206
BstYI r'GATC_y
Cuts at: 0 1278 2131 2634
Size: 1278 853 503
Fragments arranged by size:
1278 853 503
BtsI GCAGTG_nn'
Cuts at: 0 1491 2613 2634
Size: 1491 1122 21
Fragments arranged by size:
1491 1122 21
ClaI AT'CG_AT
Cuts at: 0 1532 2634
Size: 1532 1102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 431 466 2165 2200 2634
Size: 431 35 1699 35 434
Fragments arranged by size:
1699 434 431 35 35
DraI TTT'AAA
Cuts at: 0 24 587 912 2271 2634
Size: 24 563 325 1359 363
Fragments arranged by size:
1359 563 363 325 24
DrdI GACnn_nn'nnGTC
Cuts at: 0 1229 2634
Size: 1229 1405
EaeI y'GGCC_r
Cuts at: 0 99 1297 2634
Size: 99 1198 1337
Fragments arranged by size:
1337 1198 99
EarI CTCTTCn'nnn_
Cuts at: 0 1963 2634
Size: 1963 671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1569 1627 2297 2634
Size: 1022 547 58 670 337
Fragments arranged by size:
1022 670 547 337 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1423 2634
Size: 1423 1211
EcoRI G'AATT_C
Cuts at: 0 1714 2634
Size: 1714 920
EcoRV GAT'ATC
Cuts at: 0 1557 2634
Size: 1557 1077
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2327 2634
Size: 2327 307
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 612 644 660 692 1020 1052
Size: 397 32 183 32 16 32 328 32
Cuts at: 1052 1621 1638 1653 1670 1829 1861 2634
Size: 569 17 15 17 159 32 773
Fragments arranged by size:
773 569 397 328 183 159 32 32
32 32 32 17 17 16 15
MfeI C'AATT_G
Cuts at: 0 152 2634
Size: 152 2482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 1144 2634
Size: 664 480 1490
Fragments arranged by size:
1490 664 480
MscI TGG'CCA
Cuts at: 0 101 1299 2634
Size: 101 1198 1335
Fragments arranged by size:
1335 1198 101
MslI CAynn'nnrTG
Cuts at: 0 320 683 1772 2345 2634
Size: 320 363 1089 573 289
Fragments arranged by size:
1089 573 363 320 289
MspA1I CmG'CkG
Cuts at: 0 515 1130 2634
Size: 515 615 1504
Fragments arranged by size:
1504 615 515
NdeI CA'TA_TG
Cuts at: 0 2342 2634
Size: 2342 292
NsiI A_TGCA'T
Cuts at: 0 424 2463 2634
Size: 424 2039 171
Fragments arranged by size:
2039 424 171
NspI r_CATG'y
Cuts at: 0 1771 2634
Size: 1771 863
PciI A'CATG_T
Cuts at: 0 1767 2634
Size: 1767 867
PfoI T'CCnGG_A
Cuts at: 0 1038 2634
Size: 1038 1596
PvuII CAG'CTG
Cuts at: 0 515 1130 2634
Size: 515 615 1504
Fragments arranged by size:
1504 615 515
SfcI C'TryA_G
Cuts at: 0 75 2634
Size: 75 2559
SspI AAT'ATT
Cuts at: 0 651 2634
Size: 651 1983
StuI AGG'CCT
Cuts at: 0 1418 2634
Size: 1418 1216
StyI C'CwwG_G
Cuts at: 0 1820 2634
Size: 1820 814
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 205 986 1736 2108 2129 2634
Size: 205 781 750 372 21 505
Fragments arranged by size:
781 750 505 372 205 21
TatI w'GTAC_w
Cuts at: 0 1770 2305 2335 2634
Size: 1770 535 30 299
Fragments arranged by size:
1770 535 299 30
Tth111I GACn'n_nGTC
Cuts at: 0 1467 2634
Size: 1467 1167
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1997 2634
Size: 1997 637
XmnI GAAnn'nnTTC
Cuts at: 0 2369 2634
Size: 2369 265
Enzymes that do cut:
AccI AcuI AflIII AleI AloI AlwNI ApoI BamHI
BanI BbsI BciVI BclI BfrBI BglII BpmI BsaXI
BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrGI
BstAPI BstYI BtsI ClaI CspCI DraI DrdI EaeI
EarI Eco57MI EcoNI EcoRI EcoRV FalI Hin4I MfeI
MmeI MscI MslI MspA1I NdeI NsiI NspI PciI
PfoI PvuII SfcI SspI StuI StyI TaqII TatI
Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI
BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI DraIII EagI EciI EcoICRI
EcoO109I FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PasI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SwaI TstI XbaI XhoI XmaI
ZraI