Restriction map for
pF1KSDA1355
(Linear) (Six-base) MAPSORT of: KIAA1355.seq Check: 6323 from: 1 to: 3537
KIAA1355.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 622 3537
Size: 622 2915
AccI GT'mk_AC
Cuts at: 0 608 1502 2159 3537
Size: 608 894 657 1378
Fragments arranged by size:
1378 894 657 608
Acc65I G'GTAC_C
Cuts at: 0 1204 3537
Size: 1204 2333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 1767 2431 3359 3537
Size: 374 1393 664 928 178
Fragments arranged by size:
1393 928 664 374 178
AfeI AGC'GCT
Cuts at: 0 3090 3537
Size: 3090 447
AhdI GACnn_n'nnGTC
Cuts at: 0 679 1671 3505 3537
Size: 679 992 1834 32
Fragments arranged by size:
1834 992 679 32
AleI CACnn'nnGTG
Cuts at: 0 901 1537 3241 3537
Size: 901 636 1704 296
Fragments arranged by size:
1704 901 636 296
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 508 2194 3537
Size: 508 1686 1343
Fragments arranged by size:
1686 1343 508
AlwNI CAG_nnn'CTG
Cuts at: 0 450 940 1622 1687 2451 2637 2758 2778
Size: 450 490 682 65 764 186 121 20
Cuts at: 2778 3170 3537
Size: 392 367
Fragments arranged by size:
764 682 490 450 392 367 186 121
65 20
ApaI G_GGCC'C
Cuts at: 0 1217 2545 2570 3537
Size: 1217 1328 25 967
Fragments arranged by size:
1328 1217 967 25
ApoI r'AATT_y
Cuts at: 0 3271 3442 3537
Size: 3271 171 95
Fragments arranged by size:
3271 171 95
AvaI C'yCGr_G
Cuts at: 0 827 1478 1837 1907 3050 3537
Size: 827 651 359 70 1143 487
Fragments arranged by size:
1143 827 651 487 359 70
BamHI G'GATC_C
Cuts at: 0 575 836 1929 2422 2463 2493 3537
Size: 575 261 1093 493 41 30 1044
Fragments arranged by size:
1093 1044 575 493 261 41 30
BanI G'GyrC_C
Cuts at: 0 8 690 1204 1564 2046 2679 2718 3537
Size: 8 682 514 360 482 633 39 819
Fragments arranged by size:
819 682 633 514 482 360 39 8
BanII G_rGCy'C
Cuts at: 0 1217 2383 2531 2545 2570 2840 2981 3483
Size: 1217 1166 148 14 25 270 141 502
Cuts at: 3483 3537
Size: 54
Fragments arranged by size:
1217 1166 502 270 148 141 54 25
14
BbsI GAAGACnn'nnnn_
Cuts at: 0 308 362 795 2708 3370 3537
Size: 308 54 433 1913 662 167
Fragments arranged by size:
1913 662 433 308 167 54
BbvCI CC'TCA_GC
Cuts at: 0 28 749 933 2195 3142 3537
Size: 28 721 184 1262 947 395
Fragments arranged by size:
1262 947 721 395 184 28
BciVI GTATCCnnnnn_n'
Cuts at: 0 2862 2934 3537
Size: 2862 72 603
Fragments arranged by size:
2862 603 72
BclI T'GATC_A
Cuts at: 0 1139 3537
Size: 1139 2398
BglI GCCn_nnn'nGGC
Cuts at: 0 1483 1985 3537
Size: 1483 502 1552
Fragments arranged by size:
1552 1483 502
BlpI GC'TnA_GC
Cuts at: 0 3344 3537
Size: 3344 193
Bme1580I G_kGCm'C
Cuts at: 0 695 908 1126 1217 1448 1516 2545 2570
Size: 695 213 218 91 231 68 1029 25
Cuts at: 2570 2682 3537
Size: 112 855
Fragments arranged by size:
1029 855 695 231 218 213 112 91
68 25
BmgBI CAC'GTC
Cuts at: 0 506 3537
Size: 506 3031
BmrI ACTGGGnnnn_n'
Cuts at: 0 673 1458 1677 1728 2422 2666 2735 3537
Size: 673 785 219 51 694 244 69 802
Fragments arranged by size:
802 785 694 673 244 219 69 51
BmtI G_CTAG'C
Cuts at: 0 1756 3537
Size: 1756 1781
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3076 3282 3537
Size: 3076 206 255
Fragments arranged by size:
3076 255 206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 472 609 2173 2401 2545 2609 2711
Size: 262 210 137 1564 228 144 64 102
Cuts at: 2711 2755 2891 3137 3316 3353 3537
Size: 44 136 246 179 37 184
Fragments arranged by size:
1564 262 246 228 210 184 179 144
137 136 102 64 44 37
Bpu10I CC'TnA_GC
Cuts at: 0 28 749 933 2195 2382 2779 3142 3537
Size: 28 721 184 1262 187 397 363 395
Fragments arranged by size:
1262 721 397 395 363 187 184 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1225 2851 3537
Size: 1225 1626 686
Fragments arranged by size:
1626 1225 686
BsaI GGTCTCn'nnnn_
Cuts at: 0 295 3537
Size: 295 3242
BsaAI yAC'GTr
Cuts at: 0 243 1506 3537
Size: 243 1263 2031
Fragments arranged by size:
2031 1263 243
BsaWI w'CCGG_w
Cuts at: 0 1806 2146 3537
Size: 1806 340 1391
Fragments arranged by size:
1806 1391 340
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2231 2261 2753 2783 3537
Size: 2231 30 492 30 754
Fragments arranged by size:
2231 754 492 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1328 2848 3243 3388 3537
Size: 1328 1520 395 145 149
Fragments arranged by size:
1520 1328 395 149 145
BseYI C'CCAG_C
Cuts at: 0 97 114 656 872 907 963 1251 1509
Size: 97 17 542 216 35 56 288 258
Cuts at: 1509 1767 1997 2119 2434 2479 2630 2684 2839
Size: 258 230 122 315 45 151 54 155
Cuts at: 2839 2962 3170 3537
Size: 123 208 367
Fragments arranged by size:
542 367 315 288 258 258 230 216
208 155 151 123 122 97 56 54
45 35 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 577 1167 1817 2348 2834 3537
Size: 463 114 590 650 531 486 703
Fragments arranged by size:
703 650 590 531 486 463 114
BsiEI CG_ry'CG
Cuts at: 0 60 140 144 2351 3537
Size: 60 80 4 2207 1186
Fragments arranged by size:
2207 1186 80 60 4
BsiHKAI G_wGCw'C
Cuts at: 0 959 3537
Size: 959 2578
BsmI GAATG_Cn'
Cuts at: 0 1459 1661 3537
Size: 1459 202 1876
Fragments arranged by size:
1876 1459 202
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2142 3537
Size: 2142 1395
Bsp1286I G_dGCh'C
Cuts at: 0 695 908 959 1126 1217 1448 1516 2383
Size: 695 213 51 167 91 231 68 867
Cuts at: 2383 2531 2545 2570 2682 2840 2981 3483 3537
Size: 148 14 25 112 158 141 502 54
Fragments arranged by size:
867 695 502 231 213 167 158 148
141 112 91 68 54 51 25 14
BspEI T'CCGG_A
Cuts at: 0 1806 3537
Size: 1806 1731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 622 3537
Size: 134 488 2915
Fragments arranged by size:
2915 488 134
BsrBI CCG'CTC
Cuts at: 0 1266 3018 3537
Size: 1266 1752 519
Fragments arranged by size:
1752 1266 519
BsrDI GCAATG_nn'
Cuts at: 0 918 1473 3537
Size: 918 555 2064
Fragments arranged by size:
2064 918 555
BsrFI r'CCGG_y
Cuts at: 0 139 143 886 1979 2209 2266 2282 3537
Size: 139 4 743 1093 230 57 16 1255
Fragments arranged by size:
1255 1093 743 230 139 57 16 4
BssSI C'ACGA_G
Cuts at: 0 2086 3537
Size: 2086 1451
BstAPI GCAn_nnn'nTGC
Cuts at: 0 760 2371 3537
Size: 760 1611 1166
Fragments arranged by size:
1611 1166 760
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3076 3406 3537
Size: 3076 330 131
Fragments arranged by size:
3076 330 131
BstYI r'GATC_y
Cuts at: 0 575 836 1929 2325 2422 2463 2493 3537
Size: 575 261 1093 396 97 41 30 1044
Fragments arranged by size:
1093 1044 575 396 261 97 41 30
Bsu36I CC'TnA_GG
Cuts at: 0 71 3413 3537
Size: 71 3342 124
Fragments arranged by size:
3342 124 71
BtgI C'CryG_G
Cuts at: 0 480 1440 1544 1879 3537
Size: 480 960 104 335 1658
Fragments arranged by size:
1658 960 480 335 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1128 3537
Size: 1128 2409
BtsI GCAGTG_nn'
Cuts at: 0 1051 1464 1688 1901 2969 3081 3392 3537
Size: 1051 413 224 213 1068 112 311 145
Fragments arranged by size:
1068 1051 413 311 224 213 145 112
DrdI GACnn_nn'nnGTC
Cuts at: 0 2355 3537
Size: 2355 1182
EaeI y'GGCC_r
Cuts at: 0 20 137 141 531 866 1484 1637 2129
Size: 20 117 4 390 335 618 153 492
Cuts at: 2129 2207 2243 2264 3317 3537
Size: 78 36 21 1053 220
Fragments arranged by size:
1053 618 492 390 335 220 153 117
78 36 21 20 4
EagI C'GGCC_G
Cuts at: 0 137 141 3537
Size: 137 4 3396
Fragments arranged by size:
3396 137 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 568 1311 2071 2237 2308 3445 3537
Size: 568 743 760 166 71 1137 92
Fragments arranged by size:
1137 760 743 568 166 92 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 374 472 609 1767 2173 2401 2431
Size: 262 112 98 137 1158 406 228 30
Cuts at: 2431 2545 2609 2711 2755 2891 3137 3316 3353
Size: 114 64 102 44 136 246 179 37
Cuts at: 3353 3359 3537
Size: 6 178
Fragments arranged by size:
1158 406 262 246 228 179 178 137
136 114 112 102 98 64 44 37
30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 532 2059 3152 3436 3537
Size: 532 1527 1093 284 101
Fragments arranged by size:
1527 1093 532 284 101
EcoO109I rG'GnC_Cy
Cuts at: 0 269 525 673 1086 1214 1332 2512 2541
Size: 269 256 148 413 128 118 1180 29
Cuts at: 2541 2566 2755 3537
Size: 25 189 782
Fragments arranged by size:
1180 782 413 269 256 189 148 128
118 29 25
EcoRI G'AATT_C
Cuts at: 0 3271 3442 3537
Size: 3271 171 95
Fragments arranged by size:
3271 171 95
FseI GG_CCGG'CC
Cuts at: 0 143 147 3537
Size: 143 4 3390
Fragments arranged by size:
3390 143 4
HaeII r_GCGC'y
Cuts at: 0 644 2316 2556 2880 3092 3537
Size: 644 1672 240 324 212 445
Fragments arranged by size:
1672 644 445 324 240 212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 273 305 665 697 1604 1636
Size: 64 32 177 32 360 32 907 32
Cuts at: 1636 1657 1689 1848 1880 2231 2263 2414 2446
Size: 21 32 159 32 351 32 151 32
Cuts at: 2446 2485 2517 2753 2785 3491 3523 3537
Size: 39 32 236 32 706 32 14
Fragments arranged by size:
907 706 360 351 236 177 159 151
64 39 32 32 32 32 32 32
32 32 32 32 32 21 14
KpnI G_GTAC'C
Cuts at: 0 1208 3537
Size: 1208 2329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 2371 3537
Size: 416 1955 1166
Fragments arranged by size:
1955 1166 416
MscI TGG'CCA
Cuts at: 0 533 868 1486 1639 3319 3537
Size: 533 335 618 153 1680 218
Fragments arranged by size:
1680 618 533 335 218 153
MslI CAynn'nnrTG
Cuts at: 0 688 901 1009 1537 3241 3537
Size: 688 213 108 528 1704 296
Fragments arranged by size:
1704 688 528 296 213 108
MspA1I CmG'CkG
Cuts at: 0 660 779 1028 1067 1095 1831 1881 2178
Size: 660 119 249 39 28 736 50 297
Cuts at: 2178 2362 3170 3422 3537
Size: 184 808 252 115
Fragments arranged by size:
808 736 660 297 252 249 184 119
115 50 39 28
NaeI GCC'GGC
Cuts at: 0 141 145 888 1981 2211 2268 3537
Size: 141 4 743 1093 230 57 1269
Fragments arranged by size:
1269 1093 743 230 141 57 4
NgoMIV G'CCGG_C
Cuts at: 0 139 143 886 1979 2209 2266 3537
Size: 139 4 743 1093 230 57 1271
Fragments arranged by size:
1271 1093 743 230 139 57 4
NheI G'CTAG_C
Cuts at: 0 1752 3537
Size: 1752 1785
NspI r_CATG'y
Cuts at: 0 160 1014 3211 3537
Size: 160 854 2197 326
Fragments arranged by size:
2197 854 326 160
PasI CC'CwG_GG
Cuts at: 0 1119 1149 1167 1992 2930 3277 3537
Size: 1119 30 18 825 938 347 260
Fragments arranged by size:
1119 938 825 347 260 30 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 2637 2758 3170 3537
Size: 2637 121 412 367
Fragments arranged by size:
2637 412 367 121
PfoI T'CCnGG_A
Cuts at: 0 335 413 785 2757 3153 3537
Size: 335 78 372 1972 396 384
Fragments arranged by size:
1972 396 384 372 335 78
PpuMI rG'GwC_Cy
Cuts at: 0 525 673 1332 2512 2755 3537
Size: 525 148 659 1180 243 782
Fragments arranged by size:
1180 782 659 525 243 148
PspOMI G'GGCC_C
Cuts at: 0 1213 2541 2566 3537
Size: 1213 1328 25 971
Fragments arranged by size:
1328 1213 971 25
PspXI vC'TCGA_Gb
Cuts at: 0 3050 3537
Size: 3050 487
PstI C_TGCA'G
Cuts at: 0 263 824 1094 1610 1724 2976 3129 3537
Size: 263 561 270 516 114 1252 153 408
Fragments arranged by size:
1252 561 516 408 270 263 153 114
PvuII CAG'CTG
Cuts at: 0 660 779 1067 1095 1831 2178 2362 3170
Size: 660 119 288 28 736 347 184 808
Cuts at: 3170 3537
Size: 367
Fragments arranged by size:
808 736 660 367 347 288 184 119
28
SacII CC_GC'GG
Cuts at: 0 1882 3537
Size: 1882 1655
SanDI GG'GwC_CC
Cuts at: 0 1332 2512 3537
Size: 1332 1180 1025
Fragments arranged by size:
1332 1180 1025
SfcI C'TryA_G
Cuts at: 0 259 774 820 1090 1606 1720 2655 2972
Size: 259 515 46 270 516 114 935 317
Cuts at: 2972 3125 3537
Size: 153 412
Fragments arranged by size:
935 516 515 412 317 270 259 153
114 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1483 3537
Size: 1483 2054
SmaI CCC'GGG
Cuts at: 0 829 1839 1909 3537
Size: 829 1010 70 1628
Fragments arranged by size:
1628 1010 829 70
SmlI C'TyrA_G
Cuts at: 0 1240 2830 3050 3537
Size: 1240 1590 220 487
Fragments arranged by size:
1590 1240 487 220
SphI G_CATG'C
Cuts at: 0 1014 3537
Size: 1014 2523
SspI AAT'ATT
Cuts at: 0 1282 3537
Size: 1282 2255
StuI AGG'CCT
Cuts at: 0 2054 3032 3537
Size: 2054 978 505
Fragments arranged by size:
2054 978 505
StyI C'CwwG_G
Cuts at: 0 528 1073 1271 1326 1358 1430 1559 1676
Size: 528 545 198 55 32 72 129 117
Cuts at: 1676 3537
Size: 1861
Fragments arranged by size:
1861 545 528 198 129 117 72 55
32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 369 661 670 886 1659 1667 1747 2754
Size: 369 292 9 216 773 8 80 1007
Cuts at: 2754 3537
Size: 783
Fragments arranged by size:
1007 783 773 369 292 216 80 9
8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1470 1542 2148 3537
Size: 1470 72 606 1389
Fragments arranged by size:
1470 1389 606 72
XhoI C'TCGA_G
Cuts at: 0 3050 3537
Size: 3050 487
XmaI C'CCGG_G
Cuts at: 0 827 1837 1907 3537
Size: 827 1010 70 1630
Fragments arranged by size:
1630 1010 827 70
XmnI GAAnn'nnTTC
Cuts at: 0 1814 2939 3537
Size: 1814 1125 598
Fragments arranged by size:
1814 1125 598
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AhdI AleI AlfI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI
EagI EciI Eco57MI EcoNI EcoO109I EcoRI FseI HaeII
Hin4I KpnI MmeI MscI MslI MspA1I NaeI NgoMIV
NheI NspI PasI PflMI PfoI PpuMI PspOMI PspXI
PstI PvuII SacII SanDI SfcI SfiI SmaI SmlI
SphI SspI StuI StyI TaqII XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BbeI BcgI BfrBI BglII
BsaBI BsaHI BsiWI BspHI BsrGI BssHII BstBI BstEII
BstZ17I ClaI CspCI DraI DraIII EarI EcoICRI EcoRV
FalI FspI FspAI HincII HindIII HpaI KasI MfeI
MluI NarI NcoI NdeI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PsrI PvuI
RsrII SacI SalI SapI SbfI ScaI SexAI SfoI
SgfI SgrAI SnaBI SpeI SrfI SwaI TatI TstI
Tth111I XbaI ZraI