Restriction map for
pF1KSDA1360
(Linear) (Six-base) MAPSORT of: KIAA1360.seq Check: 8552 from: 1 to: 2382
KIAA1360.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AccI GT'mk_AC
Cuts at: 0 1083 2382
Size: 1083 1299
Acc65I G'GTAC_C
Cuts at: 0 738 2382
Size: 738 1644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 89 607 698 1922 2382
Size: 43 46 518 91 1224 460
Fragments arranged by size:
1224 518 460 91 46 43
AflIII A'CryG_T
Cuts at: 0 489 2382
Size: 489 1893
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1202 1234 2382
Size: 1202 32 1148
Fragments arranged by size:
1202 1148 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1680 2382
Size: 1680 702
ApaLI G'TGCA_C
Cuts at: 0 1711 2382
Size: 1711 671
ApoI r'AATT_y
Cuts at: 0 217 243 695 718 1113 1196 1229 1896
Size: 217 26 452 23 395 83 33 667
Cuts at: 1896 2382
Size: 486
Fragments arranged by size:
667 486 452 395 217 83 33 26
23
AseI AT'TA_AT
Cuts at: 0 805 2382
Size: 805 1577
BanI G'GyrC_C
Cuts at: 0 738 2382
Size: 738 1644
BanII G_rGCy'C
Cuts at: 0 995 2382
Size: 995 1387
BbsI GAAGACnn'nnnn_
Cuts at: 0 253 2382
Size: 253 2129
BbvCI CC'TCA_GC
Cuts at: 0 773 2382
Size: 773 1609
BglII A'GATC_T
Cuts at: 0 2370 2382
Size: 2370 12
Bme1580I G_kGCm'C
Cuts at: 0 1715 2382
Size: 1715 667
BmtI G_CTAG'C
Cuts at: 0 860 2357 2382
Size: 860 1497 25
Fragments arranged by size:
1497 860 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2078 2382
Size: 2078 304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 1444 2382
Size: 26 1418 938
Fragments arranged by size:
1418 938 26
Bpu10I CC'TnA_GC
Cuts at: 0 773 1128 2382
Size: 773 355 1254
Fragments arranged by size:
1254 773 355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2192 2382
Size: 2192 190
BsaBI GATnn'nnATC
Cuts at: 0 536 2382
Size: 536 1846
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1507 2195 2382
Size: 1507 688 187
Fragments arranged by size:
1507 688 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 709 2382
Size: 596 113 1673
Fragments arranged by size:
1673 596 113
BsiHKAI G_wGCw'C
Cuts at: 0 963 995 1715 2382
Size: 963 32 720 667
Fragments arranged by size:
963 720 667 32
BsmI GAATG_Cn'
Cuts at: 0 784 2382
Size: 784 1598
Bsp1286I G_dGCh'C
Cuts at: 0 963 995 1715 2382
Size: 963 32 720 667
Fragments arranged by size:
963 720 667 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1221 2382
Size: 1221 1161
BsrDI GCAATG_nn'
Cuts at: 0 2364 2382
Size: 2364 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2183 2382
Size: 2183 199
BstYI r'GATC_y
Cuts at: 0 448 983 2370 2382
Size: 448 535 1387 12
Fragments arranged by size:
1387 535 448 12
Bsu36I CC'TnA_GG
Cuts at: 0 473 2382
Size: 473 1909
BtgI C'CryG_G
Cuts at: 0 947 1847 2382
Size: 947 900 535
Fragments arranged by size:
947 900 535
BtsI GCAGTG_nn'
Cuts at: 0 99 583 1578 2382
Size: 99 484 995 804
Fragments arranged by size:
995 804 484 99
DraI TTT'AAA
Cuts at: 0 2366 2382
Size: 2366 16
EaeI y'GGCC_r
Cuts at: 0 1292 2382
Size: 1292 1090
EcoICRI GAG'CTC
Cuts at: 0 993 2382
Size: 993 1389
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 43 89 607 698 1444 1922 2382
Size: 26 17 46 518 91 746 478 460
Fragments arranged by size:
746 518 478 460 91 46 26 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2207 2382
Size: 2207 175
EcoO109I rG'GnC_Cy
Cuts at: 0 237 2382
Size: 237 2145
EcoRV GAT'ATC
Cuts at: 0 1176 2382
Size: 1176 1206
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 573 1405 2382
Size: 573 832 977
Fragments arranged by size:
977 832 573
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1024 1056 1759 1791 2382
Size: 1024 32 703 32 591
Fragments arranged by size:
1024 703 591 32 32
HincII GTy'rAC
Cuts at: 0 390 2382
Size: 390 1992
KpnI G_GTAC'C
Cuts at: 0 742 2382
Size: 742 1640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 541 1036 1977 2242 2382
Size: 407 134 495 941 265 140
Fragments arranged by size:
941 495 407 265 140 134
MslI CAynn'nnrTG
Cuts at: 0 92 1122 1762 1858 2101 2382
Size: 92 1030 640 96 243 281
Fragments arranged by size:
1030 640 281 243 96 92
MspA1I CmG'CkG
Cuts at: 0 1290 2382
Size: 1290 1092
NcoI C'CATG_G
Cuts at: 0 947 1847 2382
Size: 947 900 535
Fragments arranged by size:
947 900 535
NheI G'CTAG_C
Cuts at: 0 856 2353 2382
Size: 856 1497 29
Fragments arranged by size:
1497 856 29
NspI r_CATG'y
Cuts at: 0 493 1953 2382
Size: 493 1460 429
Fragments arranged by size:
1460 493 429
PciI A'CATG_T
Cuts at: 0 489 2382
Size: 489 1893
PfoI T'CCnGG_A
Cuts at: 0 986 2382
Size: 986 1396
PpuMI rG'GwC_Cy
Cuts at: 0 237 2382
Size: 237 2145
PsiI TTA'TAA
Cuts at: 0 23 2382
Size: 23 2359
PvuII CAG'CTG
Cuts at: 0 1290 2382
Size: 1290 1092
SacI G_AGCT'C
Cuts at: 0 995 2382
Size: 995 1387
SanDI GG'GwC_CC
Cuts at: 0 237 2382
Size: 237 2145
ScaI AGT'ACT
Cuts at: 0 1827 2382
Size: 1827 555
SfcI C'TryA_G
Cuts at: 0 1977 2382
Size: 1977 405
SmlI C'TyrA_G
Cuts at: 0 2207 2382
Size: 2207 175
SspI AAT'ATT
Cuts at: 0 277 592 1512 2382
Size: 277 315 920 870
Fragments arranged by size:
920 870 315 277
StyI C'CwwG_G
Cuts at: 0 947 1205 1847 2382
Size: 947 258 642 535
Fragments arranged by size:
947 642 535 258
TatI w'GTAC_w
Cuts at: 0 1164 1249 1825 2382
Size: 1164 85 576 557
Fragments arranged by size:
1164 576 557 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1824 1856 2382
Size: 1824 32 526
Fragments arranged by size:
1824 526 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1287 2013 2382
Size: 1287 726 369
Fragments arranged by size:
1287 726 369
Enzymes that do cut:
AccI Acc65I AcuI AflIII AloI AlwNI ApaLI ApoI
AseI BanI BanII BbsI BbvCI BglII Bme1580I BmtI
BplI BpmI Bpu10I BpuEI BsaBI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BstXI BstYI Bsu36I BtgI
BtsI DraI EaeI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI Hin4I HincII KpnI MmeI MslI MspA1I NcoI
NheI NspI PciI PfoI PpuMI PsiI PvuII SacI
SanDI ScaI SfcI SmlI SspI StyI TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI ApaI AscI AsiSI AvaI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BfrBI BglI BlpI BmgBI
BmrI BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraIII DrdI EagI EarI EciI EcoRI FseI FspI
FspAI HaeII HindIII HpaI KasI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TaqII Tth111I XbaI XhoI
XmaI XmnI ZraI