Restriction map for
pF1KA1361
(Linear) (Six-base) MAPSORT of: KIAA1361.seq Check: 7894 from: 1 to: 3003
KIAA1361.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 540 2237 3003
Size: 540 1697 766
Fragments arranged by size:
1697 766 540
AatII G_ACGT'C
Cuts at: 0 1818 3003
Size: 1818 1185
AccI GT'mk_AC
Cuts at: 0 196 3003
Size: 196 2807
Acc65I G'GTAC_C
Cuts at: 0 2859 3003
Size: 2859 144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 391 2825 3003
Size: 49 342 2434 178
Fragments arranged by size:
2434 342 178 49
AflIII A'CryG_T
Cuts at: 0 640 2990 3003
Size: 640 2350 13
Fragments arranged by size:
2350 640 13
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1973 2005 3003
Size: 1973 32 998
Fragments arranged by size:
1973 998 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2424 2904 2920 3003
Size: 2424 480 16 83
Fragments arranged by size:
2424 480 83 16
ApoI r'AATT_y
Cuts at: 0 1603 3003
Size: 1603 1400
AseI AT'TA_AT
Cuts at: 0 2336 3003
Size: 2336 667
AvaI C'yCGr_G
Cuts at: 0 1654 2712 2864 3003
Size: 1654 1058 152 139
Fragments arranged by size:
1654 1058 152 139
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1707 1740 3003
Size: 1707 33 1263
Fragments arranged by size:
1707 1263 33
BanI G'GyrC_C
Cuts at: 0 2859 3003
Size: 2859 144
BanII G_rGCy'C
Cuts at: 0 53 2057 3003
Size: 53 2004 946
Fragments arranged by size:
2004 946 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 1102 3003
Size: 1102 1901
BbvCI CC'TCA_GC
Cuts at: 0 2468 3003
Size: 2468 535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1332 1366 3003
Size: 1332 34 1637
Fragments arranged by size:
1637 1332 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2175 3003
Size: 2175 828
BclI T'GATC_A
Cuts at: 0 2030 2325 3003
Size: 2030 295 678
Fragments arranged by size:
2030 678 295
BglII A'GATC_T
Cuts at: 0 87 873 1494 3003
Size: 87 786 621 1509
Fragments arranged by size:
1509 786 621 87
BlpI GC'TnA_GC
Cuts at: 0 2315 3003
Size: 2315 688
Bme1580I G_kGCm'C
Cuts at: 0 2529 3003
Size: 2529 474
BmrI ACTGGGnnnn_n'
Cuts at: 0 2753 2772 3003
Size: 2753 19 231
Fragments arranged by size:
2753 231 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1386 3003
Size: 1386 1617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 2005 2254 2446 3003
Size: 769 1236 249 192 557
Fragments arranged by size:
1236 769 557 249 192
Bpu10I CC'TnA_GC
Cuts at: 0 2468 3003
Size: 2468 535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 2263 3003
Size: 782 1481 740
Fragments arranged by size:
1481 782 740
BsaI GGTCTCn'nnnn_
Cuts at: 0 632 1151 2517 3003
Size: 632 519 1366 486
Fragments arranged by size:
1366 632 519 486
BsaAI yAC'GTr
Cuts at: 0 289 3003
Size: 289 2714
BsaBI GATnn'nnATC
Cuts at: 0 1472 3003
Size: 1472 1531
BsaHI Gr'CG_yC
Cuts at: 0 1815 1875 3003
Size: 1815 60 1128
Fragments arranged by size:
1815 1128 60
BsaWI w'CCGG_w
Cuts at: 0 2519 3003
Size: 2519 484
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1717 1921 3003
Size: 1717 204 1082
Fragments arranged by size:
1717 1082 204
BseYI C'CCAG_C
Cuts at: 0 1063 2822 3003
Size: 1063 1759 181
Fragments arranged by size:
1759 1063 181
BsiHKAI G_wGCw'C
Cuts at: 0 53 405 2057 2258 3003
Size: 53 352 1652 201 745
Fragments arranged by size:
1652 745 352 201 53
BsmI GAATG_Cn'
Cuts at: 0 690 1846 2442 2695 3003
Size: 690 1156 596 253 308
Fragments arranged by size:
1156 690 596 308 253
Bsp1286I G_dGCh'C
Cuts at: 0 53 405 2057 2258 2529 3003
Size: 53 352 1652 201 271 474
Fragments arranged by size:
1652 474 352 271 201 53
BspEI T'CCGG_A
Cuts at: 0 2519 3003
Size: 2519 484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 540 2194 2237 3003
Size: 540 1654 43 766
Fragments arranged by size:
1654 766 540 43
BsrDI GCAATG_nn'
Cuts at: 0 695 3003
Size: 695 2308
BssSI C'ACGA_G
Cuts at: 0 134 2955 3003
Size: 134 2821 48
Fragments arranged by size:
2821 134 48
BstAPI GCAn_nnn'nTGC
Cuts at: 0 532 694 1950 3003
Size: 532 162 1256 1053
Fragments arranged by size:
1256 1053 532 162
BstBI TT'CG_AA
Cuts at: 0 1683 3003
Size: 1683 1320
BstEII G'GTnAC_C
Cuts at: 0 2833 3003
Size: 2833 170
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 2795 3003
Size: 156 2639 208
Fragments arranged by size:
2639 208 156
BstYI r'GATC_y
Cuts at: 0 66 87 328 873 1230 1251 1494 3003
Size: 66 21 241 545 357 21 243 1509
Fragments arranged by size:
1509 545 357 243 241 66 21 21
Bsu36I CC'TnA_GG
Cuts at: 0 2690 3003
Size: 2690 313
BtgI C'CryG_G
Cuts at: 0 105 521 587 2774 2827 3003
Size: 105 416 66 2187 53 176
Fragments arranged by size:
2187 416 176 105 66 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1459 3003
Size: 1459 1544
BtsI GCAGTG_nn'
Cuts at: 0 128 3003
Size: 128 2875
DraI TTT'AAA
Cuts at: 0 827 1196 3003
Size: 827 369 1807
Fragments arranged by size:
1807 827 369
DraIII CAC_nnn'GTG
Cuts at: 0 400 1167 3003
Size: 400 767 1836
Fragments arranged by size:
1836 767 400
EaeI y'GGCC_r
Cuts at: 0 102 161 1017 3003
Size: 102 59 856 1986
Fragments arranged by size:
1986 856 102 59
EarI CTCTTCn'nnn_
Cuts at: 0 58 85 410 989 1217 1589 2079 2546
Size: 58 27 325 579 228 372 490 467
Cuts at: 2546 3003
Size: 457
Fragments arranged by size:
579 490 467 457 372 325 228 58
27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1981 2925 3003
Size: 1981 944 78
Fragments arranged by size:
1981 944 78
EcoICRI GAG'CTC
Cuts at: 0 51 2055 3003
Size: 51 2004 948
Fragments arranged by size:
2004 948 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 391 769 2005 2254 2446 2825 3003
Size: 49 342 378 1236 249 192 379 178
Fragments arranged by size:
1236 379 378 342 249 192 178 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 949 3003
Size: 949 2054
EcoO109I rG'GnC_Cy
Cuts at: 0 33 1893 2755 3003
Size: 33 1860 862 248
Fragments arranged by size:
1860 862 248 33
EcoRV GAT'ATC
Cuts at: 0 453 3003
Size: 453 2550
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1711 1822 3003
Size: 1711 111 1181
Fragments arranged by size:
1711 1181 111
HaeII r_GCGC'y
Cuts at: 0 855 3003
Size: 855 2148
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 496 528 610 642 1243 1275 3003
Size: 496 32 82 32 601 32 1728
Fragments arranged by size:
1728 601 496 82 32 32 32
HincII GTy'rAC
Cuts at: 0 1104 3003
Size: 1104 1899
HindIII A'AGCT_T
Cuts at: 0 111 2627 2792 3003
Size: 111 2516 165 211
Fragments arranged by size:
2516 211 165 111
HpaI GTT'AAC
Cuts at: 0 1104 3003
Size: 1104 1899
KpnI G_GTAC'C
Cuts at: 0 2863 3003
Size: 2863 140
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1023 1983 2582 3003
Size: 1023 960 599 421
Fragments arranged by size:
1023 960 599 421
MscI TGG'CCA
Cuts at: 0 104 163 3003
Size: 104 59 2840
Fragments arranged by size:
2840 104 59
MslI CAynn'nnrTG
Cuts at: 0 1168 3003
Size: 1168 1835
MspA1I CmG'CkG
Cuts at: 0 2067 2412 3003
Size: 2067 345 591
Fragments arranged by size:
2067 591 345
NcoI C'CATG_G
Cuts at: 0 105 521 587 2774 2827 3003
Size: 105 416 66 2187 53 176
Fragments arranged by size:
2187 416 176 105 66 53
NruI TCG'CGA
Cuts at: 0 2495 3003
Size: 2495 508
NspI r_CATG'y
Cuts at: 0 644 1071 1948 2113 2994 3003
Size: 644 427 877 165 881 9
Fragments arranged by size:
881 877 644 427 165 9
PciI A'CATG_T
Cuts at: 0 640 2990 3003
Size: 640 2350 13
Fragments arranged by size:
2350 640 13
PfoI T'CCnGG_A
Cuts at: 0 950 2751 3003
Size: 950 1801 252
Fragments arranged by size:
1801 950 252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1463 1495 3003
Size: 1463 32 1508
Fragments arranged by size:
1508 1463 32
PpuMI rG'GwC_Cy
Cuts at: 0 33 1893 2755 3003
Size: 33 1860 862 248
Fragments arranged by size:
1860 862 248 33
PspXI vC'TCGA_Gb
Cuts at: 0 2864 3003
Size: 2864 139
PstI C_TGCA'G
Cuts at: 0 923 1527 2417 3003
Size: 923 604 890 586
Fragments arranged by size:
923 890 604 586
PvuII CAG'CTG
Cuts at: 0 2067 2412 3003
Size: 2067 345 591
Fragments arranged by size:
2067 591 345
SacI G_AGCT'C
Cuts at: 0 53 2057 3003
Size: 53 2004 946
Fragments arranged by size:
2004 946 53
SapI GCTCTTCn'nnn_
Cuts at: 0 58 85 410 3003
Size: 58 27 325 2593
Fragments arranged by size:
2593 325 58 27
SfcI C'TryA_G
Cuts at: 0 733 919 1523 2413 3003
Size: 733 186 604 890 590
Fragments arranged by size:
890 733 604 590 186
SmaI CCC'GGG
Cuts at: 0 2714 3003
Size: 2714 289
SmlI C'TyrA_G
Cuts at: 0 797 1654 2278 2864 3003
Size: 797 857 624 586 139
Fragments arranged by size:
857 797 624 586 139
SphI G_CATG'C
Cuts at: 0 1948 3003
Size: 1948 1055
SspI AAT'ATT
Cuts at: 0 319 3003
Size: 319 2684
StyI C'CwwG_G
Cuts at: 0 105 521 587 2774 2827 3003
Size: 105 416 66 2187 53 176
Fragments arranged by size:
2187 416 176 105 66 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2767 3003
Size: 2767 236
TatI w'GTAC_w
Cuts at: 0 2207 3003
Size: 2207 796
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2374 2406 3003
Size: 2374 32 597
Fragments arranged by size:
2374 597 32
Tth111I GACn'n_nGTC
Cuts at: 0 1897 3003
Size: 1897 1106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 112 156 543 2759 2792 3003
Size: 112 44 387 2216 33 211
Fragments arranged by size:
2216 387 211 112 44 33
XhoI C'TCGA_G
Cuts at: 0 1654 2864 3003
Size: 1654 1210 139
Fragments arranged by size:
1654 1210 139
XmaI C'CCGG_G
Cuts at: 0 2712 3003
Size: 2712 291
XmnI GAAnn'nnTTC
Cuts at: 0 79 3003
Size: 79 2924
ZraI GAC'GTC
Cuts at: 0 1816 3003
Size: 1816 1187
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AloI AlwNI
ApoI AseI AvaI BaeI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII
HindIII HpaI KpnI MmeI MscI MslI MspA1I NcoI
NruI NspI PciI PfoI PpiI PpuMI PspXI PstI
PvuII SacI SapI SfcI SmaI SmlI SphI SspI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AhdI AleI AlfI ApaI
ApaLI AscI AsiSI AvrII BamHI BbeI BfrBI BglI
BmgBI BmtI BsaXI BsgI BsiEI BsiWI BsmBI BspHI
BsrBI BsrFI BsrGI BssHII BstZ17I ClaI CspCI DrdI
EagI EcoRI FseI FspI FspAI KasI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NsiI PacI
PasI PflMI PmeI PmlI PshAI PsiI PspOMI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI StuI
SwaI XbaI