Restriction map for
pF1KSDA1363
(Linear) (Six-base) MAPSORT of: KIAA1363.seq Check: 6244 from: 1 to: 1224
KIAA1363.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 182 1224
Size: 182 1042
AccI GT'mk_AC
Cuts at: 0 56 1224
Size: 56 1168
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1224
Size: 529 695
AflIII A'CryG_T
Cuts at: 0 1030 1224
Size: 1030 194
AlwNI CAG_nnn'CTG
Cuts at: 0 957 1224
Size: 957 267
ApaI G_GGCC'C
Cuts at: 0 852 1224
Size: 852 372
ApaLI G'TGCA_C
Cuts at: 0 125 1224
Size: 125 1099
ApoI r'AATT_y
Cuts at: 0 186 473 551 1224
Size: 186 287 78 673
Fragments arranged by size:
673 287 186 78
BanII G_rGCy'C
Cuts at: 0 852 1224
Size: 852 372
BbsI GAAGACnn'nnnn_
Cuts at: 0 218 1224
Size: 218 1006
BbvCI CC'TCA_GC
Cuts at: 0 404 1224
Size: 404 820
BglI GCCn_nnn'nGGC
Cuts at: 0 34 1224
Size: 34 1190
Bme1580I G_kGCm'C
Cuts at: 0 129 852 1224
Size: 129 723 372
Fragments arranged by size:
723 372 129
BmgBI CAC'GTC
Cuts at: 0 1031 1224
Size: 1031 193
Bpu10I CC'TnA_GC
Cuts at: 0 404 467 1224
Size: 404 63 757
Fragments arranged by size:
757 404 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 1224
Size: 599 625
BsaHI Gr'CG_yC
Cuts at: 0 110 1224
Size: 110 1114
BsaWI w'CCGG_w
Cuts at: 0 1181 1224
Size: 1181 43
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 356 997 1224
Size: 356 641 227
Fragments arranged by size:
641 356 227
BseYI C'CCAG_C
Cuts at: 0 347 1224
Size: 347 877
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1224
Size: 808 416
BsiHKAI G_wGCw'C
Cuts at: 0 129 1224
Size: 129 1095
BsmI GAATG_Cn'
Cuts at: 0 420 1224
Size: 420 804
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1046 1224
Size: 1046 178
Bsp1286I G_dGCh'C
Cuts at: 0 129 852 1224
Size: 129 723 372
Fragments arranged by size:
723 372 129
BspEI T'CCGG_A
Cuts at: 0 1181 1224
Size: 1181 43
BspHI T'CATG_A
Cuts at: 0 477 1224
Size: 477 747
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 182 1224
Size: 182 1042
BsrBI CCG'CTC
Cuts at: 0 968 1224
Size: 968 256
BsrDI GCAATG_nn'
Cuts at: 0 404 800 930 980 1224
Size: 404 396 130 50 244
Fragments arranged by size:
404 396 244 130 50
BsrFI r'CCGG_y
Cuts at: 0 1090 1224
Size: 1090 134
BsrGI T'GTAC_A
Cuts at: 0 391 909 1224
Size: 391 518 315
Fragments arranged by size:
518 391 315
BstAPI GCAn_nnn'nTGC
Cuts at: 0 554 1224
Size: 554 670
BstEII G'GTnAC_C
Cuts at: 0 243 1101 1224
Size: 243 858 123
Fragments arranged by size:
858 243 123
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1080 1224
Size: 1080 144
BtgI C'CryG_G
Cuts at: 0 336 1224
Size: 336 888
BtsI GCAGTG_nn'
Cuts at: 0 1001 1224
Size: 1001 223
EaeI y'GGCC_r
Cuts at: 0 41 1224
Size: 41 1183
EarI CTCTTCn'nnn_
Cuts at: 0 299 824 1224
Size: 299 525 400
Fragments arranged by size:
525 400 299
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 960 1224
Size: 960 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1224
Size: 529 695
EcoO109I rG'GnC_Cy
Cuts at: 0 289 848 1224
Size: 289 559 376
Fragments arranged by size:
559 376 289
HaeII r_GCGC'y
Cuts at: 0 40 1224
Size: 40 1184
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 952 984 1095 1127 1224
Size: 952 32 111 32 97
Fragments arranged by size:
952 111 97 32 32
HincII GTy'rAC
Cuts at: 0 804 1224
Size: 804 420
HindIII A'AGCT_T
Cuts at: 0 650 674 1224
Size: 650 24 550
Fragments arranged by size:
650 550 24
HpaI GTT'AAC
Cuts at: 0 804 1224
Size: 804 420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 1224
Size: 107 1117
MscI TGG'CCA
Cuts at: 0 43 1224
Size: 43 1181
MspA1I CmG'CkG
Cuts at: 0 66 1224
Size: 66 1158
PflMI CCAn_nnn'nTGG
Cuts at: 0 156 1224
Size: 156 1068
PfoI T'CCnGG_A
Cuts at: 0 938 1224
Size: 938 286
PspOMI G'GGCC_C
Cuts at: 0 848 1224
Size: 848 376
PstI C_TGCA'G
Cuts at: 0 1004 1224
Size: 1004 220
SapI GCTCTTCn'nnn_
Cuts at: 0 299 1224
Size: 299 925
SfcI C'TryA_G
Cuts at: 0 1000 1224
Size: 1000 224
SmlI C'TyrA_G
Cuts at: 0 614 1224
Size: 614 610
StyI C'CwwG_G
Cuts at: 0 353 597 1224
Size: 353 244 627
Fragments arranged by size:
627 353 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 262 744 1224
Size: 262 482 480
Fragments arranged by size:
482 480 262
TatI w'GTAC_w
Cuts at: 0 391 909 1224
Size: 391 518 315
Fragments arranged by size:
518 391 315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1170 1224
Size: 1170 54
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApaI ApaLI ApoI
BanII BbsI BbvCI BglI Bme1580I BmgBI Bpu10I BpuEI
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstEII BstXI BtgI BtsI EaeI EarI EciI
Eco57MI EcoO109I HaeII Hin4I HincII HindIII HpaI MmeI
MscI MspA1I PflMI PfoI PspOMI PstI SapI SfcI
SmlI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BcgI BciVI BclI BfrBI BglII
BlpI BmrI BmtI BplI BpmI BsaI BsaAI BsaBI
BsaXI BsiEI BsiWI BssHII BssSI BstBI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
KasI KpnI MfeI MluI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI