Restriction map for
pF1KSDA1368
(Linear) (Six-base) MAPSORT of: KIAA1368.seq Check: 3320 from: 1 to: 3141
KIAA1368.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AatII G_ACGT'C
Cuts at: 0 823 3141
Size: 823 2318
AccI GT'mk_AC
Cuts at: 0 311 2060 3141
Size: 311 1749 1081
Fragments arranged by size:
1749 1081 311
AclI AA'CG_TT
Cuts at: 0 518 940 3141
Size: 518 422 2201
Fragments arranged by size:
2201 518 422
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 690 850 3070 3141
Size: 690 160 2220 71
Fragments arranged by size:
2220 690 160 71
AgeI A'CCGG_T
Cuts at: 0 2743 3141
Size: 2743 398
AlwNI CAG_nnn'CTG
Cuts at: 0 562 856 1069 1285 3141
Size: 562 294 213 216 1856
Fragments arranged by size:
1856 562 294 216 213
ApaLI G'TGCA_C
Cuts at: 0 2962 3141
Size: 2962 179
ApoI r'AATT_y
Cuts at: 0 263 472 3141
Size: 263 209 2669
Fragments arranged by size:
2669 263 209
AvaI C'yCGr_G
Cuts at: 0 2046 2721 3000 3141
Size: 2046 675 279 141
Fragments arranged by size:
2046 675 279 141
BanI G'GyrC_C
Cuts at: 0 1618 3141
Size: 1618 1523
BanII G_rGCy'C
Cuts at: 0 2123 2143 2437 3141
Size: 2123 20 294 704
Fragments arranged by size:
2123 704 294 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 1454 1910 2034 3141
Size: 1454 456 124 1107
Fragments arranged by size:
1454 1107 456 124
BciVI GTATCCnnnnn_n'
Cuts at: 0 122 441 3141
Size: 122 319 2700
Fragments arranged by size:
2700 319 122
BclI T'GATC_A
Cuts at: 0 185 2073 3141
Size: 185 1888 1068
Fragments arranged by size:
1888 1068 185
BfrBI ATG'CAT
Cuts at: 0 391 3141
Size: 391 2750
BglI GCCn_nnn'nGGC
Cuts at: 0 2572 3141
Size: 2572 569
BlpI GC'TnA_GC
Cuts at: 0 2164 3141
Size: 2164 977
Bme1580I G_kGCm'C
Cuts at: 0 1208 2966 3141
Size: 1208 1758 175
Fragments arranged by size:
1758 1208 175
BmgBI CAC'GTC
Cuts at: 0 2091 3141
Size: 2091 1050
BmrI ACTGGGnnnn_n'
Cuts at: 0 1028 3141
Size: 1028 2113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 871 878 1417 1566 2599 2620 2877
Size: 826 45 7 539 149 1033 21 257
Cuts at: 2877 3141
Size: 264
Fragments arranged by size:
1033 826 539 264 257 149 45 21
7
Bpu10I CC'TnA_GC
Cuts at: 0 764 1085 3141
Size: 764 321 2056
Fragments arranged by size:
2056 764 321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1814 3141
Size: 1814 1327
BsaI GGTCTCn'nnnn_
Cuts at: 0 1580 3141
Size: 1580 1561
BsaAI yAC'GTr
Cuts at: 0 2538 3141
Size: 2538 603
BsaBI GATnn'nnATC
Cuts at: 0 184 910 3141
Size: 184 726 2231
Fragments arranged by size:
2231 726 184
BsaHI Gr'CG_yC
Cuts at: 0 820 3141
Size: 820 2321
BsaWI w'CCGG_w
Cuts at: 0 114 593 2743 3141
Size: 114 479 2150 398
Fragments arranged by size:
2150 479 398 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 799 829 2854 2884 3141
Size: 799 30 2025 30 257
Fragments arranged by size:
2025 799 257 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1414 1730 2766 2817 2862 3141
Size: 1414 316 1036 51 45 279
Fragments arranged by size:
1414 1036 316 279 51 45
BseYI C'CCAG_C
Cuts at: 0 46 1285 1801 2359 2476 2488 2972 3010
Size: 46 1239 516 558 117 12 484 38
Cuts at: 3010 3141
Size: 131
Fragments arranged by size:
1239 558 516 484 131 117 46 38
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2122 2394 3141
Size: 2122 272 747
Fragments arranged by size:
2122 747 272
BsiHKAI G_wGCw'C
Cuts at: 0 2123 2966 3141
Size: 2123 843 175
Fragments arranged by size:
2123 843 175
BsiWI C'GTAC_G
Cuts at: 0 3055 3141
Size: 3055 86
BsmI GAATG_Cn'
Cuts at: 0 1782 1862 3141
Size: 1782 80 1279
Fragments arranged by size:
1782 1279 80
Bsp1286I G_dGCh'C
Cuts at: 0 1208 2123 2143 2437 2966 3141
Size: 1208 915 20 294 529 175
Fragments arranged by size:
1208 915 529 294 175 20
BspHI T'CATG_A
Cuts at: 0 188 3141
Size: 188 2953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2465 3141
Size: 2465 676
BsrBI CCG'CTC
Cuts at: 0 1188 3141
Size: 1188 1953
BsrDI GCAATG_nn'
Cuts at: 0 206 1001 2006 3141
Size: 206 795 1005 1135
Fragments arranged by size:
1135 1005 795 206
BsrFI r'CCGG_y
Cuts at: 0 1538 2743 3141
Size: 1538 1205 398
Fragments arranged by size:
1538 1205 398
BsrGI T'GTAC_A
Cuts at: 0 3136 3141
Size: 3136 5
BssHII G'CGCG_C
Cuts at: 0 835 3141
Size: 835 2306
BssSI C'ACGA_G
Cuts at: 0 2826 3141
Size: 2826 315
BstAPI GCAn_nnn'nTGC
Cuts at: 0 529 982 3141
Size: 529 453 2159
Fragments arranged by size:
2159 529 453
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2496 2908 2980 3141
Size: 2496 412 72 161
Fragments arranged by size:
2496 412 161 72
BstYI r'GATC_y
Cuts at: 0 790 3141
Size: 790 2351
BtgI C'CryG_G
Cuts at: 0 674 734 1225 2364 2429 2450 2646 3141
Size: 674 60 491 1139 65 21 196 495
Fragments arranged by size:
1139 674 495 491 196 65 60 21
BtsI GCAGTG_nn'
Cuts at: 0 31 725 1208 1277 1919 3141
Size: 31 694 483 69 642 1222
Fragments arranged by size:
1222 694 642 483 69 31
DraIII CAC_nnn'GTG
Cuts at: 0 1308 3141
Size: 1308 1833
EaeI y'GGCC_r
Cuts at: 0 485 1352 1536 2003 2264 2982 3141
Size: 485 867 184 467 261 718 159
Fragments arranged by size:
867 718 485 467 261 184 159
EarI CTCTTCn'nnn_
Cuts at: 0 2784 3141
Size: 2784 357
EcoICRI GAG'CTC
Cuts at: 0 2121 3141
Size: 2121 1020
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 690 826 850 871 878 1417 1566 2599
Size: 690 136 24 21 7 539 149 1033
Cuts at: 2599 2620 2877 3070 3141
Size: 21 257 193 71
Fragments arranged by size:
1033 690 539 257 193 149 136 71
24 21 21 7
EcoO109I rG'GnC_Cy
Cuts at: 0 2716 3141
Size: 2716 425
EcoRI G'AATT_C
Cuts at: 0 472 3141
Size: 472 2669
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 855 3141
Size: 855 2286
HaeII r_GCGC'y
Cuts at: 0 2578 3141
Size: 2578 563
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 677 709 2173 2205 3141
Size: 677 32 1464 32 936
Fragments arranged by size:
1464 936 677 32 32
HincII GTy'rAC
Cuts at: 0 2330 2793 3141
Size: 2330 463 348
Fragments arranged by size:
2330 463 348
MscI TGG'CCA
Cuts at: 0 487 1354 2005 2984 3141
Size: 487 867 651 979 157
Fragments arranged by size:
979 867 651 487 157
MslI CAynn'nnrTG
Cuts at: 0 175 341 1465 2959 3115 3141
Size: 175 166 1124 1494 156 26
Fragments arranged by size:
1494 1124 175 166 156 26
MspA1I CmG'CkG
Cuts at: 0 479 1282 1473 1983 2168 2366 2700 3141
Size: 479 803 191 510 185 198 334 441
Fragments arranged by size:
803 510 479 441 334 198 191 185
NcoI C'CATG_G
Cuts at: 0 734 1225 2429 2646 3141
Size: 734 491 1204 217 495
Fragments arranged by size:
1204 734 495 491 217
NdeI CA'TA_TG
Cuts at: 0 498 3141
Size: 498 2643
NsiI A_TGCA'T
Cuts at: 0 393 3141
Size: 393 2748
NspI r_CATG'y
Cuts at: 0 320 514 996 1470 2424 3141
Size: 320 194 482 474 954 717
Fragments arranged by size:
954 717 482 474 320 194
PasI CC'CwG_GG
Cuts at: 0 967 2712 3141
Size: 967 1745 429
Fragments arranged by size:
1745 967 429
PflMI CCAn_nnn'nTGG
Cuts at: 0 1597 3141
Size: 1597 1544
PfoI T'CCnGG_A
Cuts at: 0 803 855 1394 3141
Size: 803 52 539 1747
Fragments arranged by size:
1747 803 539 52
PpuMI rG'GwC_Cy
Cuts at: 0 2716 3141
Size: 2716 425
PsiI TTA'TAA
Cuts at: 0 956 3141
Size: 956 2185
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1489 1521 3141
Size: 1489 32 1620
Fragments arranged by size:
1620 1489 32
PstI C_TGCA'G
Cuts at: 0 433 978 1627 2345 3141
Size: 433 545 649 718 796
Fragments arranged by size:
796 718 649 545 433
PvuII CAG'CTG
Cuts at: 0 1282 1473 1983 3141
Size: 1282 191 510 1158
Fragments arranged by size:
1282 1158 510 191
RsrII CG'GwC_CG
Cuts at: 0 623 3141
Size: 623 2518
SacI G_AGCT'C
Cuts at: 0 2123 3141
Size: 2123 1018
SacII CC_GC'GG
Cuts at: 0 2367 3141
Size: 2367 774
SanDI GG'GwC_CC
Cuts at: 0 2716 3141
Size: 2716 425
SfcI C'TryA_G
Cuts at: 0 429 974 1623 2341 3141
Size: 429 545 649 718 800
Fragments arranged by size:
800 718 649 545 429
SmaI CCC'GGG
Cuts at: 0 2723 3141
Size: 2723 418
SmlI C'TyrA_G
Cuts at: 0 1829 3000 3141
Size: 1829 1171 141
Fragments arranged by size:
1829 1171 141
SphI G_CATG'C
Cuts at: 0 1470 3141
Size: 1470 1671
StuI AGG'CCT
Cuts at: 0 2707 3141
Size: 2707 434
StyI C'CwwG_G
Cuts at: 0 734 1225 1533 2429 2646 3141
Size: 734 491 308 896 217 495
Fragments arranged by size:
896 734 495 491 308 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1653 2140 3141
Size: 1653 487 1001
Fragments arranged by size:
1653 1001 487
TatI w'GTAC_w
Cuts at: 0 3136 3141
Size: 3136 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 799 831 2764 2796 3141
Size: 799 32 1933 32 345
Fragments arranged by size:
1933 799 345 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 583 3039 3141
Size: 583 2456 102
Fragments arranged by size:
2456 583 102
XbaI T'CTAG_A
Cuts at: 0 295 3141
Size: 295 2846
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1269 1598 3141
Size: 1269 329 1543
Fragments arranged by size:
1543 1269 329
XhoI C'TCGA_G
Cuts at: 0 3000 3141
Size: 3000 141
XmaI C'CCGG_G
Cuts at: 0 2721 3141
Size: 2721 420
XmnI GAAnn'nnTTC
Cuts at: 0 1079 3141
Size: 1079 2062
ZraI GAC'GTC
Cuts at: 0 821 3141
Size: 821 2320
Enzymes that do cut:
AatII AccI AclI AcuI AgeI AlwNI ApaLI ApoI
AvaI BanI BanII BbsI BciVI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI BtgI
BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I HincII MscI MslI MspA1I NcoI
NdeI NsiI NspI PasI PflMI PfoI PpuMI PsiI
PsrI PstI PvuII RsrII SacI SacII SanDI SfcI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AflIII AhdI AleI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BglII BmtI BplI BsiEI BsmBI
BspEI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EagI EciI EcoNI EcoRV FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NgoMIV NheI NotI NruI PacI PciI
PmeI PmlI PpiI PshAI PspOMI PspXI PvuI SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI