Restriction map for
pF1KSDA1369
(Linear) (Six-base) MAPSORT of: KIAA1369.seq Check: 6101 from: 1 to: 1890
KIAA1369.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AccI GT'mk_AC
Cuts at: 0 542 961 1890
Size: 542 419 929
Fragments arranged by size:
929 542 419
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1348 1890
Size: 646 702 542
Fragments arranged by size:
702 646 542
AflIII A'CryG_T
Cuts at: 0 705 1890
Size: 705 1185
AlwNI CAG_nnn'CTG
Cuts at: 0 1026 1731 1890
Size: 1026 705 159
Fragments arranged by size:
1026 705 159
ApaI G_GGCC'C
Cuts at: 0 100 1890
Size: 100 1790
ApaLI G'TGCA_C
Cuts at: 0 1315 1890
Size: 1315 575
ApoI r'AATT_y
Cuts at: 0 496 1262 1752 1890
Size: 496 766 490 138
Fragments arranged by size:
766 496 490 138
BamHI G'GATC_C
Cuts at: 0 411 1890
Size: 411 1479
BanII G_rGCy'C
Cuts at: 0 100 851 974 1166 1568 1890
Size: 100 751 123 192 402 322
Fragments arranged by size:
751 402 322 192 123 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 305 652 1890
Size: 305 347 1238
Fragments arranged by size:
1238 347 305
BciVI GTATCCnnnnn_n'
Cuts at: 0 445 523 1890
Size: 445 78 1367
Fragments arranged by size:
1367 445 78
BclI T'GATC_A
Cuts at: 0 1359 1890
Size: 1359 531
BglI GCCn_nnn'nGGC
Cuts at: 0 1035 1890
Size: 1035 855
BglII A'GATC_T
Cuts at: 0 1323 1890
Size: 1323 567
BlpI GC'TnA_GC
Cuts at: 0 845 1890
Size: 845 1045
Bme1580I G_kGCm'C
Cuts at: 0 100 1319 1890
Size: 100 1219 571
Fragments arranged by size:
1219 571 100
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 357 1890
Size: 357 1533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 958 988 1890
Size: 238 720 30 902
Fragments arranged by size:
902 720 238 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 976 1829 1890
Size: 246 730 853 61
Fragments arranged by size:
853 730 246 61
BsaI GGTCTCn'nnnn_
Cuts at: 0 1704 1890
Size: 1704 186
BsaAI yAC'GTr
Cuts at: 0 234 1890
Size: 234 1656
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 1069 1890
Size: 52 1017 821
Fragments arranged by size:
1017 821 52
BseYI C'CCAG_C
Cuts at: 0 52 1890
Size: 52 1838
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1123 1380 1890
Size: 1123 257 510
Fragments arranged by size:
1123 510 257
BsiHKAI G_wGCw'C
Cuts at: 0 224 770 974 1166 1319 1568 1890
Size: 224 546 204 192 153 249 322
Fragments arranged by size:
546 322 249 224 204 192 153
Bsp1286I G_dGCh'C
Cuts at: 0 100 224 770 851 974 1166 1319 1568
Size: 100 124 546 81 123 192 153 249
Cuts at: 1568 1890
Size: 322
Fragments arranged by size:
546 322 249 192 153 124 123 100
81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 646 1890
Size: 646 1244
BsrDI GCAATG_nn'
Cuts at: 0 802 1890
Size: 802 1088
BsrFI r'CCGG_y
Cuts at: 0 72 1890
Size: 72 1818
BsrGI T'GTAC_A
Cuts at: 0 1538 1890
Size: 1538 352
BssSI C'ACGA_G
Cuts at: 0 725 1890
Size: 725 1165
BstXI CCAn_nnnn'nTGG
Cuts at: 0 982 1756 1890
Size: 982 774 134
Fragments arranged by size:
982 774 134
BstYI r'GATC_y
Cuts at: 0 411 1323 1510 1890
Size: 411 912 187 380
Fragments arranged by size:
912 411 380 187
BstZ17I GTA'TAC
Cuts at: 0 543 1890
Size: 543 1347
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1688 1890
Size: 1688 202
DraIII CAC_nnn'GTG
Cuts at: 0 695 1890
Size: 695 1195
EaeI y'GGCC_r
Cuts at: 0 74 1244 1890
Size: 74 1170 646
Fragments arranged by size:
1170 646 74
EarI CTCTTCn'nnn_
Cuts at: 0 29 531 776 1438 1890
Size: 29 502 245 662 452
Fragments arranged by size:
662 502 452 245 29
EcoICRI GAG'CTC
Cuts at: 0 972 1164 1566 1890
Size: 972 192 402 324
Fragments arranged by size:
972 402 324 192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 646 958 988 1348 1890
Size: 238 408 312 30 360 542
Fragments arranged by size:
542 408 360 312 238 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 598 1890
Size: 598 1292
EcoO109I rG'GnC_Cy
Cuts at: 0 96 630 1890
Size: 96 534 1260
Fragments arranged by size:
1260 534 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 339 371 390 422 739 771 1513 1545
Size: 339 32 19 32 317 32 742 32
Cuts at: 1545 1890
Size: 345
Fragments arranged by size:
742 345 339 317 32 32 32 32
19
HincII GTy'rAC
Cuts at: 0 962 1098 1767 1890
Size: 962 136 669 123
Fragments arranged by size:
962 669 136 123
HpaI GTT'AAC
Cuts at: 0 1767 1890
Size: 1767 123
MfeI C'AATT_G
Cuts at: 0 1010 1890
Size: 1010 880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 797 1484 1890
Size: 797 687 406
Fragments arranged by size:
797 687 406
MscI TGG'CCA
Cuts at: 0 1246 1890
Size: 1246 644
MslI CAynn'nnrTG
Cuts at: 0 236 710 1147 1890
Size: 236 474 437 743
Fragments arranged by size:
743 474 437 236
MspA1I CmG'CkG
Cuts at: 0 1026 1155 1728 1890
Size: 1026 129 573 162
Fragments arranged by size:
1026 573 162 129
NaeI GCC'GGC
Cuts at: 0 74 1890
Size: 74 1816
NgoMIV G'CCGG_C
Cuts at: 0 72 1890
Size: 72 1818
NspI r_CATG'y
Cuts at: 0 709 1890
Size: 709 1181
PciI A'CATG_T
Cuts at: 0 705 1890
Size: 705 1185
PfoI T'CCnGG_A
Cuts at: 0 974 1890
Size: 974 916
PmlI CAC'GTG
Cuts at: 0 234 1890
Size: 234 1656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1589 1621 1890
Size: 1589 32 269
Fragments arranged by size:
1589 269 32
PpuMI rG'GwC_Cy
Cuts at: 0 630 1890
Size: 630 1260
PshAI GACnn'nnGTC
Cuts at: 0 1385 1890
Size: 1385 505
PspOMI G'GGCC_C
Cuts at: 0 96 1890
Size: 96 1794
PstI C_TGCA'G
Cuts at: 0 1736 1890
Size: 1736 154
PvuII CAG'CTG
Cuts at: 0 1026 1155 1728 1890
Size: 1026 129 573 162
Fragments arranged by size:
1026 573 162 129
SacI G_AGCT'C
Cuts at: 0 974 1166 1568 1890
Size: 974 192 402 322
Fragments arranged by size:
974 402 322 192
SalI G'TCGA_C
Cuts at: 0 960 1890
Size: 960 930
SfcI C'TryA_G
Cuts at: 0 1408 1732 1777 1890
Size: 1408 324 45 113
Fragments arranged by size:
1408 324 113 45
SmlI C'TyrA_G
Cuts at: 0 225 955 1844 1890
Size: 225 730 889 46
Fragments arranged by size:
889 730 225 46
SspI AAT'ATT
Cuts at: 0 675 1341 1890
Size: 675 666 549
Fragments arranged by size:
675 666 549
StyI C'CwwG_G
Cuts at: 0 464 1890
Size: 464 1426
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1537 1890
Size: 1537 353
TatI w'GTAC_w
Cuts at: 0 917 1538 1890
Size: 917 621 352
Fragments arranged by size:
917 621 352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1011 1890
Size: 1011 879
XmnI GAAnn'nnTTC
Cuts at: 0 707 1890
Size: 707 1183
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApaLI ApoI BamHI
BanII BbsI BciVI BclI BglI BglII BlpI Bme1580I
BplI BpmI BpuEI BsaI BsaAI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI BssSI BstXI
BstYI BstZ17I BtgZI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I Hin4I HincII HpaI MfeI MmeI MscI
MslI MspA1I NaeI NgoMIV NspI PciI PfoI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuII SacI SalI
SfcI SmlI SspI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BbvCI BcgI BfrBI BmgBI BmrI
BmtI Bpu10I BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BssHII BstAPI BstBI
BstEII Bsu36I BtgI BtsI ClaI CspCI DraI DrdI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII KasI KpnI MluI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PsiI PspXI PsrI PvuI RsrII SacII SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI